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Protein

[Pyruvate dehydrogenase (acetyl-transferring)] kinase 2, mitochondrial

Gene

PKP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit (PDA1), thus contributing to the regulation of glucose metabolism.3 Publications

Catalytic activityi

ATP + [pyruvate dehydrogenase (acetyl-transferring)] = ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei341ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi300 – 307ATPBy similarity8
Nucleotide bindingi359 – 360ATPBy similarity2
Nucleotide bindingi383 – 446ATPBy similarityAdd BLAST64

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: SGD
  • pyruvate dehydrogenase (acetyl-transferring) kinase activity Source: SGD

GO - Biological processi

  • carbon utilization Source: SGD
  • glucose metabolic process Source: UniProtKB-KW
  • negative regulation of catalytic activity Source: SGD
  • peptidyl-serine phosphorylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30567-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
[Pyruvate dehydrogenase (acetyl-transferring)] kinase 2, mitochondrial (EC:2.7.11.2)
Short name:
PDK 2
Short name:
Pyruvate dehydrogenase kinase 2
Alternative name(s):
Protein kinase of PDH protein 2
Pyruvate dehydrogenase complex kinase 2
Short name:
PDC kinase 2
[Pyruvate dehydrogenase [lipoamide]] kinase 2
Gene namesi
Name:PKP2
Ordered Locus Names:YGL059W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL059W.
SGDiS000003027. PKP2.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000202765? – 491[Pyruvate dehydrogenase (acetyl-transferring)] kinase 2, mitochondrial
Transit peptidei1 – ?MitochondrionSequence analysis

Proteomic databases

MaxQBiP53170.
PRIDEiP53170.

PTM databases

iPTMnetiP53170.

Interactioni

Subunit structurei

Interacts with PKP1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PKP1P405303EBI-23792,EBI-2610722

Protein-protein interaction databases

BioGridi33188. 120 interactors.
IntActiP53170. 2 interactors.
MINTiMINT-4485596.

Structurei

3D structure databases

ProteinModelPortaliP53170.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini153 – 480Histidine kinaseAdd BLAST328

Sequence similaritiesi

Belongs to the PDK/BCKDK protein kinase family.Curated
Contains 1 histidine kinase domain.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

InParanoidiP53170.
OMAiIMGALEC.
OrthoDBiEOG092C25PX.

Family and domain databases

Gene3Di1.20.140.20. 1 hit.
3.30.565.10. 2 hits.
InterProiIPR018955. BCDHK/PDK_N.
IPR003594. HATPase_C.
[Graphical view]
PfamiPF10436. BCDHK_Adom3. 1 hit.
[Graphical view]
SUPFAMiSSF55874. SSF55874. 2 hits.
SSF69012. SSF69012. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53170-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKYQINCIR YRHFLRTSNI SQIPDFTKYC IGPVNEELAP YIMETMKAYP
60 70 80 90 100
SNSEYINPQH YYHNRTVLVE NYLKRSPNPV SLTQLAQYYD DSTKLTRTKI
110 120 130 140 150
INSGKFVKEE LVIRIAHKLN QLQQLPFNVV NNFHFVQVYE SYYNIFESFR
160 170 180 190 200
KYPTIRTLED ASQFADFIKN MLEGFNTLNL PHLIMGALEC TILDLYPREK
210 220 230 240 250
MDQLLSDLLR ARISRRLIVE EHVSITANYT SGKEENTLVL GDIFQECSAK
260 270 280 290 300
KYLLEASEES QKFIQDMYFK DIPMPEFIIE GDTQLSFYFL PTHLKYLLGE
310 320 330 340 350
ILRNTYEATM KHYIRKGLEK PEPIIVTVVS NDESYLFRIS DKAGGVLHDD
360 370 380 390 400
ENLWSFGKSK ERAQESLNNF HKLPGLQTVS IYDEVHSHTK YNSKLKSLQS
410 420 430 440 450
ITLKPYMHTS LEPMSYPSII NGHIKYETPL IELLKRSFRY KLGIGLAMCK
460 470 480 490
VYAEYWNGDL SLHSMPGYGT DVVLKLGNLM KHTKKLQLDK V
Length:491
Mass (Da):57,294
Last modified:September 21, 2011 - v3
Checksum:iB3218D0207C47ADD
GO

Sequence cautioni

The sequence CAA96762 differs from that shown. Reason: Frameshift at position 435.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti224 – 225SI → VY (PubMed:9234674).Curated2
Sequence conflicti224 – 225SI → VY in CAA96762 (PubMed:9169869).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72581 Genomic DNA. Translation: CAA96762.1. Frameshift.
BK006941 Genomic DNA. Translation: DAA08043.2.
PIRiS64063.
RefSeqiNP_011456.5. NM_001180924.4.

Genome annotation databases

EnsemblFungiiYGL059W; YGL059W; YGL059W.
GeneIDi852821.
KEGGisce:YGL059W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72581 Genomic DNA. Translation: CAA96762.1. Frameshift.
BK006941 Genomic DNA. Translation: DAA08043.2.
PIRiS64063.
RefSeqiNP_011456.5. NM_001180924.4.

3D structure databases

ProteinModelPortaliP53170.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33188. 120 interactors.
IntActiP53170. 2 interactors.
MINTiMINT-4485596.

PTM databases

iPTMnetiP53170.

Proteomic databases

MaxQBiP53170.
PRIDEiP53170.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL059W; YGL059W; YGL059W.
GeneIDi852821.
KEGGisce:YGL059W.

Organism-specific databases

EuPathDBiFungiDB:YGL059W.
SGDiS000003027. PKP2.

Phylogenomic databases

InParanoidiP53170.
OMAiIMGALEC.
OrthoDBiEOG092C25PX.

Enzyme and pathway databases

BioCyciYEAST:G3O-30567-MONOMER.

Miscellaneous databases

PROiP53170.

Family and domain databases

Gene3Di1.20.140.20. 1 hit.
3.30.565.10. 2 hits.
InterProiIPR018955. BCDHK/PDK_N.
IPR003594. HATPase_C.
[Graphical view]
PfamiPF10436. BCDHK_Adom3. 1 hit.
[Graphical view]
SUPFAMiSSF55874. SSF55874. 2 hits.
SSF69012. SSF69012. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPDK2_YEAST
AccessioniPrimary (citable) accession number: P53170
Secondary accession number(s): D6VU82
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 21, 2011
Last modified: November 2, 2016
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 450 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.