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Protein

DASH complex subunit DUO1

Gene

DUO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the DASH complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The DASH complex mediates the formation and maintenance of bipolar kinetochore-microtubule attachments by forming closed rings around spindle microtubules and establishing interactions with proteins from the central kinetochore. The DASH ring complex may both stabilize microtubules during chromosome attachment in anaphase A, and allow the chromosome to remain attached to the depolymerizing microtubule in anaphase B. Microtubule depolymerization proceeds by protofilament splaying and induces the kinetochore-attached ring to slide longitudinally, thereby helping to transduce depolymerization energy into pulling forces to disjoin chromatids.4 Publications

Miscellaneous

Present with 996 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Chromosome partition, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-30569-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DASH complex subunit DUO1
Alternative name(s):
Death upon overproduction protein 1
Outer kinetochore protein DUO1
Gene namesi
Name:DUO1
Ordered Locus Names:YGL061C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL061C
SGDiS000003029 DUO1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Microtubule, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi67E → K in DUO1-1; produces abnormal spindles resulting in growth arrest at 37 degrees Celsius; when associated with V-157. 1 Publication1
Mutagenesisi117A → T in DUO1-2; produces abnormal spindles resulting in growth arrest at 37 degrees Celsius; when associated with I-124. 1 Publication1
Mutagenesisi124M → I in DUO1-2; produces abnormal spindles resulting in growth arrest at 37 degrees Celsius; when associated with T-117. 1 Publication1
Mutagenesisi157A → V in DUO1-1; produces abnormal spindles resulting in growth arrest at 37 degrees Celsius; when associated with K-67. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002155952 – 247DASH complex subunit DUO1Add BLAST246

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP53168
PaxDbiP53168
PRIDEiP53168

PTM databases

CarbonylDBiP53168
iPTMnetiP53168

Interactioni

Subunit structurei

The DASH complex is an approximately 210 kDa heterodecamer, which consists of ASK1, DAD1, DAD2, DAD3, DAD4, DAM1, DUO1, HSK3, SPC19 and SPC34, with an apparent stoichiometry of one copy of each subunit. DASH oligomerizes into a 50 nm ring composed of about 16 molecules that encircles the microtubule. Integrity of the complex and interactions with central kinetochore proteins are regulated by the spindle assembly checkpoint kinase IPL1.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi33186, 431 interactors
DIPiDIP-1287N
IntActiP53168, 23 interactors
MINTiP53168
STRINGi4932.YGL061C

Structurei

3D structure databases

ProteinModelPortaliP53168
SMRiP53168
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili152 – 180Sequence analysisAdd BLAST29

Sequence similaritiesi

Belongs to the DASH complex DUO1 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

HOGENOMiHOG000112294
InParanoidiP53168
KOiK11570
OMAiIPQIFDQ
OrthoDBiEOG092C5CMI

Family and domain databases

InterProiView protein in InterPro
IPR013960 DASH_Duo1
PfamiView protein in Pfam
PF08651 DASH_Duo1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53168-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEQSQLDDS TIDKLIPQIF NEMRSNLNNT TNKFPKSTGG GASDNISANS
60 70 80 90 100
NSIRSFNSIT TQSLLKESES LDKITAMIKN VTAALKNNLP VYVNQVHEVC
110 120 130 140 150
KSTNSILDSW INIHSQAGYI HKLMSDQTYL KLINDRLHNE NVNTNDEDGS
160 170 180 190 200
TLHNVIALKK KEILDLRQKL ENRKGEKDAA PAKPPNQGLN PRYGVQSGRR
210 220 230 240
PVPSAGISNN GRVRKTHVPA SKRPSGIPRV TNRWTKPTAS SSRKMFR
Length:247
Mass (Da):27,473
Last modified:October 1, 1996 - v1
Checksum:i787F8AF869E3C978
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72583 Genomic DNA Translation: CAA96764.1
AY558484 Genomic DNA Translation: AAS56810.1
BK006941 Genomic DNA Translation: DAA08041.1
PIRiS64065
RefSeqiNP_011454.1, NM_001180926.1

Genome annotation databases

EnsemblFungiiYGL061C; YGL061C; YGL061C
GeneIDi852819
KEGGisce:YGL061C

Similar proteinsi

Entry informationi

Entry nameiDUO1_YEAST
AccessioniPrimary (citable) accession number: P53168
Secondary accession number(s): D6VU80
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health