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P53168

- DUO1_YEAST

UniProt

P53168 - DUO1_YEAST

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Protein

DASH complex subunit DUO1

Gene
DUO1, YGL061C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the DASH complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The DASH complex mediates the formation and maintenance of bipolar kinetochore-microtubule attachments by forming closed rings around spindle microtubules and establishing interactions with proteins from the central kinetochore. The DASH ring complex may both stabilize microtubules during chromosome attachment in anaphase A, and allow the chromosome to remain attached to the depolymerizing microtubule in anaphase B. Microtubule depolymerization proceeds by protofilament splaying and induces the kinetochore-attached ring to slide longitudinally, thereby helping to transduce depolymerization energy into pulling forces to disjoin chromatids.4 Publications

GO - Molecular functioni

  1. protein binding Source: IntAct

GO - Biological processi

  1. attachment of spindle microtubules to kinetochore Source: SGD
  2. mitotic nuclear division Source: UniProtKB-KW
  3. positive regulation of attachment of spindle microtubules to kinetochore Source: SGD
  4. positive regulation of microtubule polymerization Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Chromosome partition, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-30569-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DASH complex subunit DUO1
Alternative name(s):
Death upon overproduction protein 1
Outer kinetochore protein DUO1
Gene namesi
Name:DUO1
Ordered Locus Names:YGL061C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGL061c.
SGDiS000003029. DUO1.

Subcellular locationi

Nucleus. Cytoplasmcytoskeletonspindle. Chromosomecentromerekinetochore
Note: Associates with the mitotic spindle and the kinetochore.2 Publications

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. DASH complex Source: SGD
  3. microtubule Source: UniProtKB-KW
  4. mitotic spindle Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Microtubule, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi67 – 671E → K in DUO1-1; produces abnormal spindles resulting in growth arrest at 37 degrees Celsius; when associated with V-157. 1 Publication
Mutagenesisi117 – 1171A → T in DUO1-2; produces abnormal spindles resulting in growth arrest at 37 degrees Celsius; when associated with I-124. 1 Publication
Mutagenesisi124 – 1241M → I in DUO1-2; produces abnormal spindles resulting in growth arrest at 37 degrees Celsius; when associated with T-117. 1 Publication
Mutagenesisi157 – 1571A → V in DUO1-1; produces abnormal spindles resulting in growth arrest at 37 degrees Celsius; when associated with K-67. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 247246DASH complex subunit DUO1PRO_0000215595Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP53168.
PaxDbiP53168.
PeptideAtlasiP53168.

Expressioni

Gene expression databases

GenevestigatoriP53168.

Interactioni

Subunit structurei

The DASH complex is an approximately 210 kDa heterodecamer, which consists of ASK1, DAD1, DAD2, DAD3, DAD4, DAM1, DUO1, HSK3, SPC19 and SPC34, with an apparent stoichiometry of one copy of each subunit. DASH oligomerizes into a 50 nm ring composed of about 16 molecules that encircles the microtubule. Integrity of the complex and interactions with central kinetochore proteins are regulated by the spindle assembly checkpoint kinase IPL1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
DAD1Q122484EBI-23800,EBI-35662
DAM1P532676EBI-23800,EBI-23268
SPC34P361313EBI-23800,EBI-26401

Protein-protein interaction databases

BioGridi33186. 162 interactions.
DIPiDIP-1287N.
IntActiP53168. 7 interactions.
MINTiMINT-405719.
STRINGi4932.YGL061C.

Structurei

3D structure databases

ProteinModelPortaliP53168.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili152 – 18029 Reviewed predictionAdd
BLAST

Sequence similaritiesi

Belongs to the DASH complex DUO1 family.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG42829.
HOGENOMiHOG000112294.
KOiK11570.
OMAiHEVCKST.
OrthoDBiEOG74FFDB.

Family and domain databases

InterProiIPR013960. DASH_Duo1.
[Graphical view]
PfamiPF08651. DASH_Duo1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53168-1 [UniParc]FASTAAdd to Basket

« Hide

MSEQSQLDDS TIDKLIPQIF NEMRSNLNNT TNKFPKSTGG GASDNISANS    50
NSIRSFNSIT TQSLLKESES LDKITAMIKN VTAALKNNLP VYVNQVHEVC 100
KSTNSILDSW INIHSQAGYI HKLMSDQTYL KLINDRLHNE NVNTNDEDGS 150
TLHNVIALKK KEILDLRQKL ENRKGEKDAA PAKPPNQGLN PRYGVQSGRR 200
PVPSAGISNN GRVRKTHVPA SKRPSGIPRV TNRWTKPTAS SSRKMFR 247
Length:247
Mass (Da):27,473
Last modified:October 1, 1996 - v1
Checksum:i787F8AF869E3C978
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z72583 Genomic DNA. Translation: CAA96764.1.
AY558484 Genomic DNA. Translation: AAS56810.1.
BK006941 Genomic DNA. Translation: DAA08041.1.
PIRiS64065.
RefSeqiNP_011454.1. NM_001180926.1.

Genome annotation databases

EnsemblFungiiYGL061C; YGL061C; YGL061C.
GeneIDi852819.
KEGGisce:YGL061C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z72583 Genomic DNA. Translation: CAA96764.1 .
AY558484 Genomic DNA. Translation: AAS56810.1 .
BK006941 Genomic DNA. Translation: DAA08041.1 .
PIRi S64065.
RefSeqi NP_011454.1. NM_001180926.1.

3D structure databases

ProteinModelPortali P53168.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33186. 162 interactions.
DIPi DIP-1287N.
IntActi P53168. 7 interactions.
MINTi MINT-405719.
STRINGi 4932.YGL061C.

Proteomic databases

MaxQBi P53168.
PaxDbi P53168.
PeptideAtlasi P53168.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGL061C ; YGL061C ; YGL061C .
GeneIDi 852819.
KEGGi sce:YGL061C.

Organism-specific databases

CYGDi YGL061c.
SGDi S000003029. DUO1.

Phylogenomic databases

eggNOGi NOG42829.
HOGENOMi HOG000112294.
KOi K11570.
OMAi HEVCKST.
OrthoDBi EOG74FFDB.

Enzyme and pathway databases

BioCyci YEAST:G3O-30569-MONOMER.

Miscellaneous databases

NextBioi 972363.

Gene expression databases

Genevestigatori P53168.

Family and domain databases

InterProi IPR013960. DASH_Duo1.
[Graphical view ]
Pfami PF08651. DASH_Duo1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The characterization of two new clusters of duplicated genes suggests a 'Lego' organization of the yeast Saccharomyces cerevisiae chromosomes."
    Feuermann M., de Montigny J., Potier S., Souciet J.-L.
    Yeast 13:861-869(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Saccharomyces cerevisiae Duo1p and Dam1p, novel proteins involved in mitotic spindle function."
    Hofmann C., Cheeseman I.M., Goode B.L., McDonald K.L., Barnes G., Drubin D.G.
    J. Cell Biol. 143:1029-1040(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF GLU-67; ALA-117; MET-124 AND ALA-157.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Formation of a dynamic kinetochore-microtubule interface through assembly of the Dam1 ring complex."
    Westermann S., Avila-Sakar A., Wang H.-W., Niederstrasser H., Wong J., Drubin D.G., Nogales E., Barnes G.
    Mol. Cell 17:277-290(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends."
    Westermann S., Wang H.-W., Avila-Sakar A., Drubin D.G., Nogales E., Barnes G.
    Nature 440:565-569(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Molecular architecture of a kinetochore-microtubule attachment site."
    Joglekar A.P., Bouck D.C., Molk J.N., Bloom K.S., Salmon E.D.
    Nat. Cell Biol. 8:581-585(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  11. "The Dam1 kinetochore complex harnesses microtubule dynamics to produce force and movement."
    Asbury C.L., Gestaut D.R., Powers A.F., Franck A.D., Davis T.N.
    Proc. Natl. Acad. Sci. U.S.A. 103:9873-9878(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  13. "The yeast DASH complex forms closed rings on microtubules."
    Miranda J.L., Wulf P.D., Sorger P.K., Harrison S.C.
    Nat. Struct. Mol. Biol. 12:138-143(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: ELECTRON MICROSCOPY OF DASH COMPLEX ALONE AND BOUND TO MICROTUBULES.

Entry informationi

Entry nameiDUO1_YEAST
AccessioniPrimary (citable) accession number: P53168
Secondary accession number(s): D6VU80
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 3, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 996 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

Similar proteinsi