ID   PUS2_YEAST              Reviewed;         370 AA.
AC   P53167; D6VU78; Q06713;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   21-SEP-2011, sequence version 4.
DT   27-MAR-2024, entry version 166.
DE   RecName: Full=tRNA pseudouridine(27/28) synthase;
DE            EC=5.4.99.44;
DE   AltName: Full=tRNA pseudouridine synthase 2;
DE   AltName: Full=tRNA pseudouridylate synthase 2;
DE   AltName: Full=tRNA-uridine isomerase 2;
GN   Name=PUS2; OrderedLocusNames=YGL063W;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8641292; DOI=10.1002/j.1460-2075.1996.tb00580.x;
RA   Simos G., Tekotte H., Grosjean H., Segref A., Sharma K., Tollervey D.,
RA   Hurt E.C.;
RT   "Nuclear pore proteins are involved in the biogenesis of functional tRNA.";
RL   EMBO J. 15:2270-2284(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9234674;
RX   DOI=10.1002/(sici)1097-0061(199707)13:9<861::aid-yea125>3.0.co;2-9;
RA   Feuermann M., de Montigny J., Potier S., Souciet J.-L.;
RT   "The characterization of two new clusters of duplicated genes suggests a
RT   'Lego' organization of the yeast Saccharomyces cerevisiae chromosomes.";
RL   Yeast 13:861-869(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169869;
RA   Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA   Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA   Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA   Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA   Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA   Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA   Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA   Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA   Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA   Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA   Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA   Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA   Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA   Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA   Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA   Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA   Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA   Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA   Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA   Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA   Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA   Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL   Nature 387:81-84(1997).
RN   [4]
RP   GENOME REANNOTATION, AND SEQUENCE REVISION TO 136.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [5]
RP   FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, AND MUTAGENESIS OF
RP   ASP-56.
RX   PubMed=17684231; DOI=10.1261/rna.605607;
RA   Behm-Ansmant I., Branlant C., Motorin Y.;
RT   "The Saccharomyces cerevisiae Pus2 protein encoded by YGL063w ORF is a
RT   mitochondrial tRNA:Psi27/28-synthase.";
RL   RNA 13:1641-1647(2007).
CC   -!- FUNCTION: Mitochondrial-specific pseudouridine synthase catalyzing the
CC       formation of pseudouridine at positions 27 and 28 in the anticodon stem
CC       and loop of mitochondrial transfer RNAs. {ECO:0000269|PubMed:17684231}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(27/28) in mitochondrial tRNA = pseudouridine(27/28) in
CC         mitochondrial tRNA; Xref=Rhea:RHEA:42560, Rhea:RHEA-COMP:10115,
CC         Rhea:RHEA-COMP:10116, ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC         EC=5.4.99.44; Evidence={ECO:0000269|PubMed:17684231};
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:17684231}.
CC   -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X80674; CAA56699.1; -; Genomic_DNA.
DR   EMBL; Z72585; CAA96766.1; -; Genomic_DNA.
DR   EMBL; BK006941; DAA08039.2; -; Genomic_DNA.
DR   PIR; S64067; S64067.
DR   RefSeq; NP_011452.4; NM_001180928.4.
DR   AlphaFoldDB; P53167; -.
DR   SMR; P53167; -.
DR   BioGRID; 33184; 75.
DR   DIP; DIP-5022N; -.
DR   IntAct; P53167; 1.
DR   MINT; P53167; -.
DR   STRING; 4932.YGL063W; -.
DR   MaxQB; P53167; -.
DR   PaxDb; 4932-YGL063W; -.
DR   PeptideAtlas; P53167; -.
DR   EnsemblFungi; YGL063W_mRNA; YGL063W; YGL063W.
DR   GeneID; 852817; -.
DR   KEGG; sce:YGL063W; -.
DR   AGR; SGD:S000003031; -.
DR   SGD; S000003031; PUS2.
DR   VEuPathDB; FungiDB:YGL063W; -.
DR   eggNOG; KOG2553; Eukaryota.
DR   GeneTree; ENSGT00950000183160; -.
DR   HOGENOM; CLU_021971_4_0_1; -.
DR   InParanoid; P53167; -.
DR   OMA; CDARTYT; -.
DR   OrthoDB; 12038at2759; -.
DR   BioCyc; MetaCyc:YGL063W-MONOMER; -.
DR   BioCyc; YEAST:YGL063W-MONOMER; -.
DR   BRENDA; 5.4.99.44; 984.
DR   BioGRID-ORCS; 852817; 1 hit in 10 CRISPR screens.
DR   PRO; PR:P53167; -.
DR   Proteomes; UP000002311; Chromosome VII.
DR   RNAct; P53167; Protein.
DR   GO; GO:0005739; C:mitochondrion; IDA:SGD.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IMP:SGD.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:1990481; P:mRNA pseudouridine synthesis; IMP:SGD.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IMP:SGD.
DR   CDD; cd02568; PseudoU_synth_PUS1_PUS2; 1.
DR   Gene3D; 3.30.70.660; Pseudouridine synthase I, catalytic domain, C-terminal subdomain; 1.
DR   Gene3D; 3.30.70.580; Pseudouridine synthase I, catalytic domain, N-terminal subdomain; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR001406; PsdUridine_synth_TruA.
DR   InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR   InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR   InterPro; IPR041708; PUS1/PUS2-like.
DR   InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR   NCBIfam; TIGR00071; hisT_truA; 1.
DR   PANTHER; PTHR11142; PSEUDOURIDYLATE SYNTHASE; 1.
DR   PANTHER; PTHR11142:SF4; PSEUDOURIDYLATE SYNTHASE 1 HOMOLOG; 1.
DR   Pfam; PF01416; PseudoU_synth_1; 1.
DR   SUPFAM; SSF55120; Pseudouridine synthase; 1.
PE   1: Evidence at protein level;
KW   Isomerase; Mitochondrion; Reference proteome; tRNA processing.
FT   CHAIN           1..370
FT                   /note="tRNA pseudouridine(27/28) synthase"
FT                   /id="PRO_0000057532"
FT   ACT_SITE        56
FT                   /note="Nucleophile"
FT   BINDING         111
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   MUTAGEN         56
FT                   /note="D->A: Abolishes catalytic activity."
FT                   /evidence="ECO:0000269|PubMed:17684231"
FT   CONFLICT        136
FT                   /note="C -> S (in Ref. 2 and 3; CAA96766)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   370 AA;  41891 MW;  776D3A5D8C83EBC3 CRC64;
     MLLGYCGSGY YGMQYNPPHK TIEGEILTKL FDVGAISEEN SLAPKKNSFM AAARTDKGVH
     AMLNLLSLKI TLREDTVAKL NAALPPEIRV WGIQPVNKKF NARSACDSRW YQYLIPEFIL
     IGPPRSSLLH RNVGGCYRED GSQEVWDTFL EQTRGRFSGD ELCRLQDTAQ KLSESDPLVQ
     DYVGLLSGTL SGYCLSPSKL DAFEAAMQEY VGTHNFHNFT TGKLWGDPSA QRHIKKVVVS
     QASPGWICVR IHGQSFMLHQ IRRMVALAVL AARCQLPPNI VRNYFNAGPR KYIPRAPAQG
     LLLEGPVFDG YNTKLRNLLY CEIRPDDITL ERMCRFRERQ ICTAIAHEET QRHVFCHFVR
     QMNRLATPLI
//