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Protein

tRNA pseudouridine(27/28) synthase

Gene

PUS2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mitochondrial-specific pseudouridine synthase catalyzing the formation of pseudouridine at positions 27 and 28 in the anticodon stem and loop of mitochondrial transfer RNAs.1 Publication

Catalytic activityi

Mitochondrial tRNA uridine(27/28) = mitochondrial tRNA pseudouridine(27/28).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei56Nucleophile1
Binding sitei111SubstrateBy similarity1

GO - Molecular functioni

  • pseudouridine synthase activity Source: SGD
  • RNA binding Source: InterPro

GO - Biological processi

  • mRNA pseudouridine synthesis Source: SGD
  • tRNA pseudouridine synthesis Source: SGD

Keywordsi

Molecular functionIsomerase
Biological processtRNA processing

Enzyme and pathway databases

BioCyciMetaCyc:YGL063W-MONOMER
YEAST:YGL063W-MONOMER
BRENDAi5.4.99.44 984

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA pseudouridine(27/28) synthase (EC:5.4.99.44)
Alternative name(s):
tRNA pseudouridine synthase 2
tRNA pseudouridylate synthase 2
tRNA-uridine isomerase 2
Gene namesi
Name:PUS2
Ordered Locus Names:YGL063W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL063W
SGDiS000003031 PUS2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi56D → A: Abolishes catalytic activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000575321 – 370tRNA pseudouridine(27/28) synthaseAdd BLAST370

Proteomic databases

MaxQBiP53167
PaxDbiP53167
PRIDEiP53167

Interactioni

Protein-protein interaction databases

BioGridi33184, 70 interactors
DIPiDIP-5022N
IntActiP53167, 3 interactors
MINTiP53167
STRINGi4932.YGL063W

Structurei

3D structure databases

ProteinModelPortaliP53167
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00510000047706
HOGENOMiHOG000213984
InParanoidiP53167
KOiK15452
OMAiKNKCDAR
OrthoDBiEOG092C2LVK

Family and domain databases

Gene3Di3.30.70.660, 1 hit
InterProiView protein in InterPro
IPR020103 PsdUridine_synth_cat_dom_sf
IPR001406 PsdUridine_synth_TruA
IPR020097 PsdUridine_synth_TruA_a/b_dom
IPR020095 PsdUridine_synth_TruA_C
PANTHERiPTHR11142 PTHR11142, 1 hit
PfamiView protein in Pfam
PF01416 PseudoU_synth_1, 1 hit
SUPFAMiSSF55120 SSF55120, 2 hits
TIGRFAMsiTIGR00071 hisT_truA, 1 hit

Sequencei

Sequence statusi: Complete.

P53167-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLGYCGSGY YGMQYNPPHK TIEGEILTKL FDVGAISEEN SLAPKKNSFM
60 70 80 90 100
AAARTDKGVH AMLNLLSLKI TLREDTVAKL NAALPPEIRV WGIQPVNKKF
110 120 130 140 150
NARSACDSRW YQYLIPEFIL IGPPRSSLLH RNVGGCYRED GSQEVWDTFL
160 170 180 190 200
EQTRGRFSGD ELCRLQDTAQ KLSESDPLVQ DYVGLLSGTL SGYCLSPSKL
210 220 230 240 250
DAFEAAMQEY VGTHNFHNFT TGKLWGDPSA QRHIKKVVVS QASPGWICVR
260 270 280 290 300
IHGQSFMLHQ IRRMVALAVL AARCQLPPNI VRNYFNAGPR KYIPRAPAQG
310 320 330 340 350
LLLEGPVFDG YNTKLRNLLY CEIRPDDITL ERMCRFRERQ ICTAIAHEET
360 370
QRHVFCHFVR QMNRLATPLI
Length:370
Mass (Da):41,891
Last modified:September 21, 2011 - v4
Checksum:i776D3A5D8C83EBC3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti136C → S (PubMed:9234674).Curated1
Sequence conflicti136C → S in CAA96766 (PubMed:9169869).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80674 Genomic DNA Translation: CAA56699.1
Z72585 Genomic DNA Translation: CAA96766.1
BK006941 Genomic DNA Translation: DAA08039.2
PIRiS64067
RefSeqiNP_011452.4, NM_001180928.4

Genome annotation databases

EnsemblFungiiYGL063W; YGL063W; YGL063W
GeneIDi852817
KEGGisce:YGL063W

Similar proteinsi

Entry informationi

Entry nameiPUS2_YEAST
AccessioniPrimary (citable) accession number: P53167
Secondary accession number(s): D6VU78, Q06713
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 21, 2011
Last modified: March 28, 2018
This is version 136 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health