P53167 (PUS2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA pseudouridine(27/28) synthase
EC=5.4.99.44

Alternative name(s):
tRNA pseudouridine synthase 2
tRNA pseudouridylate synthase 2
tRNA-uridine isomerase 2

Gene names

Name:	PUS2
Ordered Locus Names:	YGL063W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mitochondrial-specific pseudouridine synthase catalyzing the formation of pseudouridine at positions 27 and 28 in the anticodon stem and loop of mitochondrial transfer RNAs. Ref.5
Catalytic activity	Mitochondrial tRNA uridine(27/28) = mitochondrial tRNA pseudouridine(27/28). Ref.5
Subcellular location	Mitochondrion Ref.5.
Sequence similarities	Belongs to the tRNA pseudouridine synthase TruA family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Mitochondrion
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tRNA pseudouridine synthesis Inferred from mutant phenotype Ref.5. Source: SGD
Cellular_component	mitochondrion Inferred from direct assay Ref.5. Source: SGD
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro pseudouridine synthase activity Inferred from mutant phenotype Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 370	370	tRNA pseudouridine(27/28) synthase		PRO_0000057532
Sites
	Active site	56	1	Nucleophile
	Binding site	111	1	Substrate By similarity
Experimental info
	Mutagenesis	56	1	D → A: Abolishes catalytic activity. Ref.5
	Sequence conflict	136	1	C → S Ref.2
	Sequence conflict	136	1	C → S in CAA96766. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P53167 [UniParc]. Last modified September 21, 2011. Version 4. Checksum: 776D3A5D8C83EBC3	FASTA	370	41,891
	10 20 30 40 50 60 MLLGYCGSGY YGMQYNPPHK TIEGEILTKL FDVGAISEEN SLAPKKNSFM AAARTDKGVH 70 80 90 100 110 120 AMLNLLSLKI TLREDTVAKL NAALPPEIRV WGIQPVNKKF NARSACDSRW YQYLIPEFIL 130 140 150 160 170 180 IGPPRSSLLH RNVGGCYRED GSQEVWDTFL EQTRGRFSGD ELCRLQDTAQ KLSESDPLVQ 190 200 210 220 230 240 DYVGLLSGTL SGYCLSPSKL DAFEAAMQEY VGTHNFHNFT TGKLWGDPSA QRHIKKVVVS 250 260 270 280 290 300 QASPGWICVR IHGQSFMLHQ IRRMVALAVL AARCQLPPNI VRNYFNAGPR KYIPRAPAQG 310 320 330 340 350 360 LLLEGPVFDG YNTKLRNLLY CEIRPDDITL ERMCRFRERQ ICTAIAHEET QRHVFCHFVR 370 QMNRLATPLI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nuclear pore proteins are involved in the biogenesis of functional tRNA." Simos G., Tekotte H., Grosjean H., Segref A., Sharma K., Tollervey D., Hurt E.C. EMBO J. 15:2270-2284(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The characterization of two new clusters of duplicated genes suggests a 'Lego' organization of the yeast Saccharomyces cerevisiae chromosomes." Feuermann M., de Montigny J., Potier S., Souciet J.-L. Yeast 13:861-869(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 136. Strain: ATCC 204508 / S288c.
[5]	"The Saccharomyces cerevisiae Pus2 protein encoded by YGL063w ORF is a mitochondrial tRNA:Psi27/28-synthase." Behm-Ansmant I., Branlant C., Motorin Y. RNA 13:1641-1647(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, MUTAGENESIS OF ASP-56.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X80674 Genomic DNA. Translation: CAA56699.1. Z72585 Genomic DNA. Translation: CAA96766.1. BK006941 Genomic DNA. Translation: DAA08039.2.
PIR	S64067.
RefSeq	NP_011452.4. NM_001180928.4.
3D structure databases
ProteinModelPortal	P53167.
SMR	P53167. Positions 5-115.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5022N.
MINT	MINT-499274.
STRING	4932.YGL063W.
Proteomic databases
PaxDb	P53167.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL063W; YGL063W; YGL063W.
GeneID	852817.
KEGG	sce:YGL059W. sce:YGL063W.
Organism-specific databases
CYGD	YGL063w.
SGD	S000003031. PUS2.
Phylogenomic databases
eggNOG	COG0101.
GeneTree	ENSGT00510000047706.
HOGENOM	HOG000213984.
KO	K15452.
OMA	CFISSES.
OrthoDB	EOG48KVMJ.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-16706.
Gene expression databases
Genevestigator	P53167.
GermOnline	YGL063W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.30.70.580. 1 hit. 3.30.70.660. 1 hit.
InterPro	IPR020103. PsdUridine_synth_cat_dom. IPR001406. PsdUridine_synth_TruA. IPR020097. PsdUridine_synth_TruA_a/b_dom. IPR020095. PsdUridine_synth_TruA_C. IPR020094. PsdUridine_synth_TruA_N. [Graphical view]
PANTHER	PTHR11142. PTHR11142. 1 hit.
Pfam	PF01416. PseudoU_synth_1. 2 hits. [Graphical view]
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit.
TIGRFAMs	TIGR00071. hisT_truA. 1 hit.
ProtoNet	Search...
Other
NextBio	972357.

Entry information

Entry name

PUS2_YEAST

Accession

Primary (citable) accession number: P53167
Secondary accession number(s): D6VU78, Q06713

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	September 21, 2011
Last modified:	May 1, 2013
This is version 100 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program