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P53167

- PUS2_YEAST

UniProt

P53167 - PUS2_YEAST

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Protein
tRNA pseudouridine(27/28) synthase
Gene
PUS2, YGL063W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Mitochondrial-specific pseudouridine synthase catalyzing the formation of pseudouridine at positions 27 and 28 in the anticodon stem and loop of mitochondrial transfer RNAs.1 Publication

Catalytic activityi

Mitochondrial tRNA uridine(27/28) = mitochondrial tRNA pseudouridine(27/28).1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei56 – 561Nucleophile
Binding sitei111 – 1111Substrate By similarity

GO - Molecular functioni

  1. RNA binding Source: InterPro
  2. pseudouridine synthase activity Source: SGD

GO - Biological processi

  1. tRNA pseudouridine synthesis Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16706.
YEAST:YGL063W-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA pseudouridine(27/28) synthase (EC:5.4.99.44)
Alternative name(s):
tRNA pseudouridine synthase 2
tRNA pseudouridylate synthase 2
tRNA-uridine isomerase 2
Gene namesi
Name:PUS2
Ordered Locus Names:YGL063W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGL063w.
SGDiS000003031. PUS2.

Subcellular locationi

Mitochondrion 1 Publication

GO - Cellular componenti

  1. mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi56 – 561D → A: Abolishes catalytic activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 370370tRNA pseudouridine(27/28) synthase
PRO_0000057532Add
BLAST

Proteomic databases

MaxQBiP53167.
PaxDbiP53167.

Expressioni

Gene expression databases

GenevestigatoriP53167.

Interactioni

Protein-protein interaction databases

BioGridi33184. 32 interactions.
DIPiDIP-5022N.
MINTiMINT-499274.
STRINGi4932.YGL063W.

Structurei

3D structure databases

ProteinModelPortaliP53167.
SMRiP53167. Positions 1-304.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0101.
GeneTreeiENSGT00510000047706.
HOGENOMiHOG000213984.
KOiK15452.
OMAiCFISSES.
OrthoDBiEOG7DVDMQ.

Family and domain databases

Gene3Di3.30.70.580. 1 hit.
3.30.70.660. 1 hit.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view]
PANTHERiPTHR11142. PTHR11142. 1 hit.
PfamiPF01416. PseudoU_synth_1. 2 hits.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 2 hits.
TIGRFAMsiTIGR00071. hisT_truA. 1 hit.

Sequencei

Sequence statusi: Complete.

P53167-1 [UniParc]FASTAAdd to Basket

« Hide

MLLGYCGSGY YGMQYNPPHK TIEGEILTKL FDVGAISEEN SLAPKKNSFM    50
AAARTDKGVH AMLNLLSLKI TLREDTVAKL NAALPPEIRV WGIQPVNKKF 100
NARSACDSRW YQYLIPEFIL IGPPRSSLLH RNVGGCYRED GSQEVWDTFL 150
EQTRGRFSGD ELCRLQDTAQ KLSESDPLVQ DYVGLLSGTL SGYCLSPSKL 200
DAFEAAMQEY VGTHNFHNFT TGKLWGDPSA QRHIKKVVVS QASPGWICVR 250
IHGQSFMLHQ IRRMVALAVL AARCQLPPNI VRNYFNAGPR KYIPRAPAQG 300
LLLEGPVFDG YNTKLRNLLY CEIRPDDITL ERMCRFRERQ ICTAIAHEET 350
QRHVFCHFVR QMNRLATPLI 370
Length:370
Mass (Da):41,891
Last modified:September 21, 2011 - v4
Checksum:i776D3A5D8C83EBC3
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti136 – 1361C → S1 Publication
Sequence conflicti136 – 1361C → S in CAA96766. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X80674 Genomic DNA. Translation: CAA56699.1.
Z72585 Genomic DNA. Translation: CAA96766.1.
BK006941 Genomic DNA. Translation: DAA08039.2.
PIRiS64067.
RefSeqiNP_011452.4. NM_001180928.4.

Genome annotation databases

EnsemblFungiiYGL063W; YGL063W; YGL063W.
GeneIDi852817.
KEGGisce:YGL063W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X80674 Genomic DNA. Translation: CAA56699.1 .
Z72585 Genomic DNA. Translation: CAA96766.1 .
BK006941 Genomic DNA. Translation: DAA08039.2 .
PIRi S64067.
RefSeqi NP_011452.4. NM_001180928.4.

3D structure databases

ProteinModelPortali P53167.
SMRi P53167. Positions 1-304.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33184. 32 interactions.
DIPi DIP-5022N.
MINTi MINT-499274.
STRINGi 4932.YGL063W.

Proteomic databases

MaxQBi P53167.
PaxDbi P53167.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGL063W ; YGL063W ; YGL063W .
GeneIDi 852817.
KEGGi sce:YGL063W.

Organism-specific databases

CYGDi YGL063w.
SGDi S000003031. PUS2.

Phylogenomic databases

eggNOGi COG0101.
GeneTreei ENSGT00510000047706.
HOGENOMi HOG000213984.
KOi K15452.
OMAi CFISSES.
OrthoDBi EOG7DVDMQ.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-16706.
YEAST:YGL063W-MONOMER.

Miscellaneous databases

NextBioi 972357.
PROi P53167.

Gene expression databases

Genevestigatori P53167.

Family and domain databases

Gene3Di 3.30.70.580. 1 hit.
3.30.70.660. 1 hit.
InterProi IPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view ]
PANTHERi PTHR11142. PTHR11142. 1 hit.
Pfami PF01416. PseudoU_synth_1. 2 hits.
[Graphical view ]
SUPFAMi SSF55120. SSF55120. 2 hits.
TIGRFAMsi TIGR00071. hisT_truA. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nuclear pore proteins are involved in the biogenesis of functional tRNA."
    Simos G., Tekotte H., Grosjean H., Segref A., Sharma K., Tollervey D., Hurt E.C.
    EMBO J. 15:2270-2284(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The characterization of two new clusters of duplicated genes suggests a 'Lego' organization of the yeast Saccharomyces cerevisiae chromosomes."
    Feuermann M., de Montigny J., Potier S., Souciet J.-L.
    Yeast 13:861-869(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 136.
    Strain: ATCC 204508 / S288c.
  5. "The Saccharomyces cerevisiae Pus2 protein encoded by YGL063w ORF is a mitochondrial tRNA:Psi27/28-synthase."
    Behm-Ansmant I., Branlant C., Motorin Y.
    RNA 13:1641-1647(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, MUTAGENESIS OF ASP-56.

Entry informationi

Entry nameiPUS2_YEAST
AccessioniPrimary (citable) accession number: P53167
Secondary accession number(s): D6VU78, Q06713
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 21, 2011
Last modified: July 9, 2014
This is version 109 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

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