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P53167 (PUS2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA pseudouridine(27/28) synthase

EC=5.4.99.44
Alternative name(s):
tRNA pseudouridine synthase 2
tRNA pseudouridylate synthase 2
tRNA-uridine isomerase 2
Gene names
Name:PUS2
Ordered Locus Names:YGL063W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length370 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mitochondrial-specific pseudouridine synthase catalyzing the formation of pseudouridine at positions 27 and 28 in the anticodon stem and loop of mitochondrial transfer RNAs. Ref.5

Catalytic activity

Mitochondrial tRNA uridine(27/28) = mitochondrial tRNA pseudouridine(27/28). Ref.5

Subcellular location

Mitochondrion Ref.5.

Sequence similarities

Belongs to the tRNA pseudouridine synthase TruA family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentMitochondrion
   Molecular functionIsomerase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtRNA pseudouridine synthesis

Inferred from mutant phenotype Ref.5. Source: SGD

   Cellular_componentmitochondrion

Inferred from direct assay Ref.5. Source: SGD

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: InterPro

pseudouridine synthase activity

Inferred from mutant phenotype Ref.5. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 370370tRNA pseudouridine(27/28) synthase
PRO_0000057532

Sites

Active site561Nucleophile
Binding site1111Substrate By similarity

Experimental info

Mutagenesis561D → A: Abolishes catalytic activity. Ref.5
Sequence conflict1361C → S Ref.2
Sequence conflict1361C → S in CAA96766. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P53167 [UniParc].

Last modified September 21, 2011. Version 4.
Checksum: 776D3A5D8C83EBC3

FASTA37041,891
        10         20         30         40         50         60 
MLLGYCGSGY YGMQYNPPHK TIEGEILTKL FDVGAISEEN SLAPKKNSFM AAARTDKGVH 

        70         80         90        100        110        120 
AMLNLLSLKI TLREDTVAKL NAALPPEIRV WGIQPVNKKF NARSACDSRW YQYLIPEFIL 

       130        140        150        160        170        180 
IGPPRSSLLH RNVGGCYRED GSQEVWDTFL EQTRGRFSGD ELCRLQDTAQ KLSESDPLVQ 

       190        200        210        220        230        240 
DYVGLLSGTL SGYCLSPSKL DAFEAAMQEY VGTHNFHNFT TGKLWGDPSA QRHIKKVVVS 

       250        260        270        280        290        300 
QASPGWICVR IHGQSFMLHQ IRRMVALAVL AARCQLPPNI VRNYFNAGPR KYIPRAPAQG 

       310        320        330        340        350        360 
LLLEGPVFDG YNTKLRNLLY CEIRPDDITL ERMCRFRERQ ICTAIAHEET QRHVFCHFVR 

       370 
QMNRLATPLI 

« Hide

References

« Hide 'large scale' references
[1]"Nuclear pore proteins are involved in the biogenesis of functional tRNA."
Simos G., Tekotte H., Grosjean H., Segref A., Sharma K., Tollervey D., Hurt E.C.
EMBO J. 15:2270-2284(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The characterization of two new clusters of duplicated genes suggests a 'Lego' organization of the yeast Saccharomyces cerevisiae chromosomes."
Feuermann M., de Montigny J., Potier S., Souciet J.-L.
Yeast 13:861-869(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 136.
Strain: ATCC 204508 / S288c.
[5]"The Saccharomyces cerevisiae Pus2 protein encoded by YGL063w ORF is a mitochondrial tRNA:Psi27/28-synthase."
Behm-Ansmant I., Branlant C., Motorin Y.
RNA 13:1641-1647(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, MUTAGENESIS OF ASP-56.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X80674 Genomic DNA. Translation: CAA56699.1.
Z72585 Genomic DNA. Translation: CAA96766.1.
BK006941 Genomic DNA. Translation: DAA08039.2.
PIRS64067.
RefSeqNP_011452.4. NM_001180928.4.

3D structure databases

ProteinModelPortalP53167.
SMRP53167. Positions 1-304.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33184. 32 interactions.
DIPDIP-5022N.
MINTMINT-499274.
STRING4932.YGL063W.

Proteomic databases

MaxQBP53167.
PaxDbP53167.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGL063W; YGL063W; YGL063W.
GeneID852817.
KEGGsce:YGL063W.

Organism-specific databases

CYGDYGL063w.
SGDS000003031. PUS2.

Phylogenomic databases

eggNOGCOG0101.
GeneTreeENSGT00510000047706.
HOGENOMHOG000213984.
KOK15452.
OMACFISSES.
OrthoDBEOG7DVDMQ.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-16706.
YEAST:YGL063W-MONOMER.

Gene expression databases

GenevestigatorP53167.

Family and domain databases

Gene3D3.30.70.580. 1 hit.
3.30.70.660. 1 hit.
InterProIPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view]
PANTHERPTHR11142. PTHR11142. 1 hit.
PfamPF01416. PseudoU_synth_1. 2 hits.
[Graphical view]
SUPFAMSSF55120. SSF55120. 2 hits.
TIGRFAMsTIGR00071. hisT_truA. 1 hit.
ProtoNetSearch...

Other

NextBio972357.
PROP53167.

Entry information

Entry namePUS2_YEAST
AccessionPrimary (citable) accession number: P53167
Secondary accession number(s): D6VU78, Q06713
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 21, 2011
Last modified: July 9, 2014
This is version 109 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families