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P53167

- PUS2_YEAST

UniProt

P53167 - PUS2_YEAST

Protein

tRNA pseudouridine(27/28) synthase

Gene

PUS2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 4 (21 Sep 2011)
      Previous versions | rss
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    Functioni

    Mitochondrial-specific pseudouridine synthase catalyzing the formation of pseudouridine at positions 27 and 28 in the anticodon stem and loop of mitochondrial transfer RNAs.1 Publication

    Catalytic activityi

    Mitochondrial tRNA uridine(27/28) = mitochondrial tRNA pseudouridine(27/28).1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei56 – 561Nucleophile
    Binding sitei111 – 1111SubstrateBy similarity

    GO - Molecular functioni

    1. pseudouridine synthase activity Source: SGD
    2. RNA binding Source: InterPro

    GO - Biological processi

    1. tRNA pseudouridine synthesis Source: SGD

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    tRNA processing

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-16706.
    YEAST:YGL063W-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA pseudouridine(27/28) synthase (EC:5.4.99.44)
    Alternative name(s):
    tRNA pseudouridine synthase 2
    tRNA pseudouridylate synthase 2
    tRNA-uridine isomerase 2
    Gene namesi
    Name:PUS2
    Ordered Locus Names:YGL063W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGL063w.
    SGDiS000003031. PUS2.

    Subcellular locationi

    Mitochondrion 1 Publication

    GO - Cellular componenti

    1. mitochondrion Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi56 – 561D → A: Abolishes catalytic activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 370370tRNA pseudouridine(27/28) synthasePRO_0000057532Add
    BLAST

    Proteomic databases

    MaxQBiP53167.
    PaxDbiP53167.

    Expressioni

    Gene expression databases

    GenevestigatoriP53167.

    Interactioni

    Protein-protein interaction databases

    BioGridi33184. 32 interactions.
    DIPiDIP-5022N.
    MINTiMINT-499274.
    STRINGi4932.YGL063W.

    Structurei

    3D structure databases

    ProteinModelPortaliP53167.
    SMRiP53167. Positions 1-304.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0101.
    GeneTreeiENSGT00510000047706.
    HOGENOMiHOG000213984.
    KOiK15452.
    OMAiCFISSES.
    OrthoDBiEOG7DVDMQ.

    Family and domain databases

    Gene3Di3.30.70.580. 1 hit.
    3.30.70.660. 1 hit.
    InterProiIPR020103. PsdUridine_synth_cat_dom.
    IPR001406. PsdUridine_synth_TruA.
    IPR020097. PsdUridine_synth_TruA_a/b_dom.
    IPR020095. PsdUridine_synth_TruA_C.
    IPR020094. PsdUridine_synth_TruA_N.
    [Graphical view]
    PANTHERiPTHR11142. PTHR11142. 1 hit.
    PfamiPF01416. PseudoU_synth_1. 2 hits.
    [Graphical view]
    SUPFAMiSSF55120. SSF55120. 2 hits.
    TIGRFAMsiTIGR00071. hisT_truA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P53167-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLLGYCGSGY YGMQYNPPHK TIEGEILTKL FDVGAISEEN SLAPKKNSFM    50
    AAARTDKGVH AMLNLLSLKI TLREDTVAKL NAALPPEIRV WGIQPVNKKF 100
    NARSACDSRW YQYLIPEFIL IGPPRSSLLH RNVGGCYRED GSQEVWDTFL 150
    EQTRGRFSGD ELCRLQDTAQ KLSESDPLVQ DYVGLLSGTL SGYCLSPSKL 200
    DAFEAAMQEY VGTHNFHNFT TGKLWGDPSA QRHIKKVVVS QASPGWICVR 250
    IHGQSFMLHQ IRRMVALAVL AARCQLPPNI VRNYFNAGPR KYIPRAPAQG 300
    LLLEGPVFDG YNTKLRNLLY CEIRPDDITL ERMCRFRERQ ICTAIAHEET 350
    QRHVFCHFVR QMNRLATPLI 370
    Length:370
    Mass (Da):41,891
    Last modified:September 21, 2011 - v4
    Checksum:i776D3A5D8C83EBC3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti136 – 1361C → S(PubMed:9234674)Curated
    Sequence conflicti136 – 1361C → S in CAA96766. (PubMed:9169869)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X80674 Genomic DNA. Translation: CAA56699.1.
    Z72585 Genomic DNA. Translation: CAA96766.1.
    BK006941 Genomic DNA. Translation: DAA08039.2.
    PIRiS64067.
    RefSeqiNP_011452.4. NM_001180928.4.

    Genome annotation databases

    EnsemblFungiiYGL063W; YGL063W; YGL063W.
    GeneIDi852817.
    KEGGisce:YGL063W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X80674 Genomic DNA. Translation: CAA56699.1 .
    Z72585 Genomic DNA. Translation: CAA96766.1 .
    BK006941 Genomic DNA. Translation: DAA08039.2 .
    PIRi S64067.
    RefSeqi NP_011452.4. NM_001180928.4.

    3D structure databases

    ProteinModelPortali P53167.
    SMRi P53167. Positions 1-304.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33184. 32 interactions.
    DIPi DIP-5022N.
    MINTi MINT-499274.
    STRINGi 4932.YGL063W.

    Proteomic databases

    MaxQBi P53167.
    PaxDbi P53167.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL063W ; YGL063W ; YGL063W .
    GeneIDi 852817.
    KEGGi sce:YGL063W.

    Organism-specific databases

    CYGDi YGL063w.
    SGDi S000003031. PUS2.

    Phylogenomic databases

    eggNOGi COG0101.
    GeneTreei ENSGT00510000047706.
    HOGENOMi HOG000213984.
    KOi K15452.
    OMAi CFISSES.
    OrthoDBi EOG7DVDMQ.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-16706.
    YEAST:YGL063W-MONOMER.

    Miscellaneous databases

    NextBioi 972357.
    PROi P53167.

    Gene expression databases

    Genevestigatori P53167.

    Family and domain databases

    Gene3Di 3.30.70.580. 1 hit.
    3.30.70.660. 1 hit.
    InterProi IPR020103. PsdUridine_synth_cat_dom.
    IPR001406. PsdUridine_synth_TruA.
    IPR020097. PsdUridine_synth_TruA_a/b_dom.
    IPR020095. PsdUridine_synth_TruA_C.
    IPR020094. PsdUridine_synth_TruA_N.
    [Graphical view ]
    PANTHERi PTHR11142. PTHR11142. 1 hit.
    Pfami PF01416. PseudoU_synth_1. 2 hits.
    [Graphical view ]
    SUPFAMi SSF55120. SSF55120. 2 hits.
    TIGRFAMsi TIGR00071. hisT_truA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nuclear pore proteins are involved in the biogenesis of functional tRNA."
      Simos G., Tekotte H., Grosjean H., Segref A., Sharma K., Tollervey D., Hurt E.C.
      EMBO J. 15:2270-2284(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The characterization of two new clusters of duplicated genes suggests a 'Lego' organization of the yeast Saccharomyces cerevisiae chromosomes."
      Feuermann M., de Montigny J., Potier S., Souciet J.-L.
      Yeast 13:861-869(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 136.
      Strain: ATCC 204508 / S288c.
    5. "The Saccharomyces cerevisiae Pus2 protein encoded by YGL063w ORF is a mitochondrial tRNA:Psi27/28-synthase."
      Behm-Ansmant I., Branlant C., Motorin Y.
      RNA 13:1641-1647(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, MUTAGENESIS OF ASP-56.

    Entry informationi

    Entry nameiPUS2_YEAST
    AccessioniPrimary (citable) accession number: P53167
    Secondary accession number(s): D6VU78, Q06713
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: September 21, 2011
    Last modified: October 1, 2014
    This is version 110 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3