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Protein

ATP-dependent RNA helicase MRH4, mitochondrial

Gene

MRH4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ATP-binding RNA helicase involved in mitochondrial RNA metabolism. Required for maintenance of mitochondrial DNA.2 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi164 – 1718ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATP-dependent RNA helicase activity Source: SGD
  3. mitochondrial ribosomal large subunit rRNA binding Source: SGD

GO - Biological processi

  1. mitochondrial large ribosomal subunit assembly Source: SGD
  2. RNA metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30570-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase MRH4, mitochondrial (EC:3.6.4.13)
Alternative name(s):
Mitochondrial RNA helicase 4
Gene namesi
Name:MRH4
Ordered Locus Names:YGL064C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGL064c.
SGDiS000003032. MRH4.

Subcellular locationi

Mitochondrion 3 Publications

GO - Cellular componenti

  1. mitochondrial matrix Source: SGD
  2. mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2626MitochondrionSequence AnalysisAdd
BLAST
Chaini27 – 561535ATP-dependent RNA helicase MRH4, mitochondrialPRO_0000041929Add
BLAST

Proteomic databases

MaxQBiP53166.
PaxDbiP53166.

Expressioni

Gene expression databases

GenevestigatoriP53166.

Interactioni

Protein-protein interaction databases

BioGridi33183. 39 interactions.
DIPiDIP-6672N.
IntActiP53166. 4 interactions.
MINTiMINT-622031.
STRINGi4932.YGL064C.

Structurei

3D structure databases

ProteinModelPortaliP53166.
SMRiP53166. Positions 97-556.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini151 – 339189Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini370 – 561192Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi117 – 14125Q motifAdd
BLAST
Motifi287 – 2904DEAD box

Domaini

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0513.
GeneTreeiENSGT00780000122753.
HOGENOMiHOG000065970.
InParanoidiP53166.
KOiK17678.
OMAiKLMEDSP.
OrthoDBiEOG79W9DT.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53166-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLFFKPVIS PQWSFPVLLK IGVRSYAGGP RTKHKGNSPL ASVPTGSSNK
60 70 80 90 100
NRKQKAKGKK GNKKNDPDQA FNFGEYGGLK KDVEMNMDST NKLIQKISNF
110 120 130 140 150
DQLLILPPVR DAVKEIISKE SLKLQDSRKK TSENIIPSPI QTVAIKRISK
160 170 180 190 200
NLMDPKLQIH AIAAETGSGK TMAYLIPLID YLKRQELETP ELWETLRKNV
210 220 230 240 250
LIRSIILVPT HELVDQVYET VSKTKTLLGL NSFKWDKATS YRDLLENIKN
260 270 280 290 300
RIDILVTTPG KLLNLFSIRM ITRPDKVLSK VGFVVLDEAD TLLDRSWLEE
310 320 330 340 350
THSAIKRIPN INHLIFCSAT IPQEFNKTMQ RLFPTVVPIM TPRLHKLPFA
360 370 380 390 400
LDFKVINSAL SPFKGSKIKA LAQTLYAISN DDTEPGFEKR CIIFVNEKKN
410 420 430 440 450
VPEIVNLLNK KFGHNAIGLT GEDTFEERSE KIMPFLSPPR PLSEVVAQST
460 470 480 490 500
SPPTSLKKFE IPDSNIVIGK LKNTNSNGTA PSNKSLHVLV TTDLMARGLN
510 520 530 540 550
FKGVRNVVLY DVPKTSIDLI HRVGRTARMK QGGRVFMLTD SKTKSWAKAL
560
PKIIKKHQRL S
Length:561
Mass (Da):63,058
Last modified:October 1, 1996 - v1
Checksum:iA8CEE92D461DB6EC
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti67 – 671P → S in strain: DBY947. 1 Publication
Natural varianti438 – 4381P → S in strain: DBY947. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72586 Genomic DNA. Translation: CAA96767.1.
BK006941 Genomic DNA. Translation: DAA08038.1.
PIRiS64068.
RefSeqiNP_011451.1. NM_001180929.1.

Genome annotation databases

EnsemblFungiiYGL064C; YGL064C; YGL064C.
GeneIDi852816.
KEGGisce:YGL064C.

Cross-referencesi

Web resourcesi

RNA helicases from the baker's yeast Saccharomyces cerevisiae

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72586 Genomic DNA. Translation: CAA96767.1.
BK006941 Genomic DNA. Translation: DAA08038.1.
PIRiS64068.
RefSeqiNP_011451.1. NM_001180929.1.

3D structure databases

ProteinModelPortaliP53166.
SMRiP53166. Positions 97-556.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33183. 39 interactions.
DIPiDIP-6672N.
IntActiP53166. 4 interactions.
MINTiMINT-622031.
STRINGi4932.YGL064C.

Proteomic databases

MaxQBiP53166.
PaxDbiP53166.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL064C; YGL064C; YGL064C.
GeneIDi852816.
KEGGisce:YGL064C.

Organism-specific databases

CYGDiYGL064c.
SGDiS000003032. MRH4.

Phylogenomic databases

eggNOGiCOG0513.
GeneTreeiENSGT00780000122753.
HOGENOMiHOG000065970.
InParanoidiP53166.
KOiK17678.
OMAiKLMEDSP.
OrthoDBiEOG79W9DT.

Enzyme and pathway databases

BioCyciYEAST:G3O-30570-MONOMER.

Miscellaneous databases

NextBioi972354.

Gene expression databases

GenevestigatoriP53166.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The characterization of two new clusters of duplicated genes suggests a 'Lego' organization of the yeast Saccharomyces cerevisiae chromosomes."
    Feuermann M., de Montigny J., Potier S., Souciet J.-L.
    Yeast 13:861-869(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Systematic identification, classification, and characterization of the open reading frames which encode novel helicase-related proteins in Saccharomyces cerevisiae by gene disruption and Northern analysis."
    Shiratori A., Shibata T., Arisawa M., Hanaoka F., Murakami Y., Eki T.
    Yeast 15:219-253(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "A novel mitochondrial DEAD box protein (Mrh4) required for maintenance of mtDNA in Saccharomyces cerevisiae."
    Schmidt U., Lehmann K., Stahl U.
    FEMS Yeast Res. 2:267-276(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, VARIANTS SER-67 AND SER-438.
    Strain: DBY947.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.

Entry informationi

Entry nameiMRH4_YEAST
AccessioniPrimary (citable) accession number: P53166
Secondary accession number(s): D6VU77
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 4, 2015
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 736 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.