P53166 (MRH4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent RNA helicase MRH4, mitochondrial
EC=3.6.4.13

Alternative name(s):
Mitochondrial RNA helicase 4

Gene names

Name:	MRH4
Ordered Locus Names:	YGL064C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	561 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	ATP-binding RNA helicase involved in mitochondrial RNA metabolism. Required for maintenance of mitochondrial DNA. Ref.4 Ref.5
Catalytic activity	ATP + H2O = ADP + phosphate.
Subcellular location	Mitochondrion Ref.5 Ref.6 Ref.8.
Domain	The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.
Miscellaneous	Present with 736 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the DEAD box helicase family. MRH4 subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA metabolic process Inferred from mutant phenotype Ref.5. Source: SGD
Cellular_component	mitochondrion Inferred from direct assay Ref.5Ref.8PubMed 16823961. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 26	26	Mitochondrion Potential
	Chain	27 – 561	535	ATP-dependent RNA helicase MRH4, mitochondrial		PRO_0000041929
Regions
	Domain	151 – 339	189	Helicase ATP-binding
	Domain	370 – 561	192	Helicase C-terminal
	Nucleotide binding	164 – 171	8	ATP By similarity
	Motif	117 – 141	25	Q motif
	Motif	287 – 290	4	DEAD box
Natural variations
	Natural variant	67	1	P → S in strain: DBY947. Ref.5
	Natural variant	438	1	P → S in strain: DBY947. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P53166 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: A8CEE92D461DB6EC	FASTA	561	63,058
	10 20 30 40 50 60 MSLFFKPVIS PQWSFPVLLK IGVRSYAGGP RTKHKGNSPL ASVPTGSSNK NRKQKAKGKK 70 80 90 100 110 120 GNKKNDPDQA FNFGEYGGLK KDVEMNMDST NKLIQKISNF DQLLILPPVR DAVKEIISKE 130 140 150 160 170 180 SLKLQDSRKK TSENIIPSPI QTVAIKRISK NLMDPKLQIH AIAAETGSGK TMAYLIPLID 190 200 210 220 230 240 YLKRQELETP ELWETLRKNV LIRSIILVPT HELVDQVYET VSKTKTLLGL NSFKWDKATS 250 260 270 280 290 300 YRDLLENIKN RIDILVTTPG KLLNLFSIRM ITRPDKVLSK VGFVVLDEAD TLLDRSWLEE 310 320 330 340 350 360 THSAIKRIPN INHLIFCSAT IPQEFNKTMQ RLFPTVVPIM TPRLHKLPFA LDFKVINSAL 370 380 390 400 410 420 SPFKGSKIKA LAQTLYAISN DDTEPGFEKR CIIFVNEKKN VPEIVNLLNK KFGHNAIGLT 430 440 450 460 470 480 GEDTFEERSE KIMPFLSPPR PLSEVVAQST SPPTSLKKFE IPDSNIVIGK LKNTNSNGTA 490 500 510 520 530 540 PSNKSLHVLV TTDLMARGLN FKGVRNVVLY DVPKTSIDLI HRVGRTARMK QGGRVFMLTD 550 560 SKTKSWAKAL PKIIKKHQRL S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The characterization of two new clusters of duplicated genes suggests a 'Lego' organization of the yeast Saccharomyces cerevisiae chromosomes." Feuermann M., de Montigny J., Potier S., Souciet J.-L. Yeast 13:861-869(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Systematic identification, classification, and characterization of the open reading frames which encode novel helicase-related proteins in Saccharomyces cerevisiae by gene disruption and Northern analysis." Shiratori A., Shibata T., Arisawa M., Hanaoka F., Murakami Y., Eki T. Yeast 15:219-253(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"A novel mitochondrial DEAD box protein (Mrh4) required for maintenance of mtDNA in Saccharomyces cerevisiae." Schmidt U., Lehmann K., Stahl U. FEMS Yeast Res. 2:267-276(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, VARIANTS SER-67 AND SER-438. Strain: DBY947.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
+	Additional computationally mapped references.

Web resources

RNA helicases from the baker's yeast Saccharomyces cerevisiae

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z72586 Genomic DNA. Translation: CAA96767.1. BK006941 Genomic DNA. Translation: DAA08038.1.
PIR	S64068.
RefSeq	NP_011451.1. NM_001180929.1.
3D structure databases
ProteinModelPortal	P53166.
SMR	P53166. Positions 97-556.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6672N.
IntAct	P53166. 4 interactions.
MINT	MINT-622031.
STRING	4932.YGL064C.
Proteomic databases
PaxDb	P53166.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL064C; YGL064C; YGL064C.
GeneID	852816.
KEGG	sce:YGL064C.
Organism-specific databases
CYGD	YGL064c.
SGD	S000003032. MRH4.
Phylogenomic databases
eggNOG	COG0513.
GeneTree	ENSGT00700000105515.
HOGENOM	HOG000065970.
OMA	SGKTWAY.
OrthoDB	EOG47M56K.
Gene expression databases
Genevestigator	P53166.
GermOnline	YGL064C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. [Graphical view]
Pfam	PF00270. DEAD. 1 hit. PF00271. Helicase_C. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS00039. DEAD_ATP_HELICASE. False negative. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	972354.

Entry information

Entry name

MRH4_YEAST

Accession

Primary (citable) accession number: P53166
Secondary accession number(s): D6VU77

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 115 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program