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P53166 (MRH4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent RNA helicase MRH4, mitochondrial

EC=3.6.4.13
Alternative name(s):
Mitochondrial RNA helicase 4
Gene names
Name:MRH4
Ordered Locus Names:YGL064C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length561 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

ATP-binding RNA helicase involved in mitochondrial RNA metabolism. Required for maintenance of mitochondrial DNA. Ref.4 Ref.5

Catalytic activity

ATP + H2O = ADP + phosphate.

Subcellular location

Mitochondrion Ref.5 Ref.6 Ref.8.

Domain

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Miscellaneous

Present with 736 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the DEAD box helicase family. MRH4 subfamily.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2626Mitochondrion Potential
Chain27 – 561535ATP-dependent RNA helicase MRH4, mitochondrial
PRO_0000041929

Regions

Domain151 – 339189Helicase ATP-binding
Domain370 – 561192Helicase C-terminal
Nucleotide binding164 – 1718ATP By similarity
Motif117 – 14125Q motif
Motif287 – 2904DEAD box

Natural variations

Natural variant671P → S in strain: DBY947. Ref.5
Natural variant4381P → S in strain: DBY947. Ref.5

Sequences

Sequence LengthMass (Da)Tools
P53166 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: A8CEE92D461DB6EC

FASTA56163,058
        10         20         30         40         50         60 
MSLFFKPVIS PQWSFPVLLK IGVRSYAGGP RTKHKGNSPL ASVPTGSSNK NRKQKAKGKK 

        70         80         90        100        110        120 
GNKKNDPDQA FNFGEYGGLK KDVEMNMDST NKLIQKISNF DQLLILPPVR DAVKEIISKE 

       130        140        150        160        170        180 
SLKLQDSRKK TSENIIPSPI QTVAIKRISK NLMDPKLQIH AIAAETGSGK TMAYLIPLID 

       190        200        210        220        230        240 
YLKRQELETP ELWETLRKNV LIRSIILVPT HELVDQVYET VSKTKTLLGL NSFKWDKATS 

       250        260        270        280        290        300 
YRDLLENIKN RIDILVTTPG KLLNLFSIRM ITRPDKVLSK VGFVVLDEAD TLLDRSWLEE 

       310        320        330        340        350        360 
THSAIKRIPN INHLIFCSAT IPQEFNKTMQ RLFPTVVPIM TPRLHKLPFA LDFKVINSAL 

       370        380        390        400        410        420 
SPFKGSKIKA LAQTLYAISN DDTEPGFEKR CIIFVNEKKN VPEIVNLLNK KFGHNAIGLT 

       430        440        450        460        470        480 
GEDTFEERSE KIMPFLSPPR PLSEVVAQST SPPTSLKKFE IPDSNIVIGK LKNTNSNGTA 

       490        500        510        520        530        540 
PSNKSLHVLV TTDLMARGLN FKGVRNVVLY DVPKTSIDLI HRVGRTARMK QGGRVFMLTD 

       550        560 
SKTKSWAKAL PKIIKKHQRL S 

« Hide

References

« Hide 'large scale' references
[1]"The characterization of two new clusters of duplicated genes suggests a 'Lego' organization of the yeast Saccharomyces cerevisiae chromosomes."
Feuermann M., de Montigny J., Potier S., Souciet J.-L.
Yeast 13:861-869(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Systematic identification, classification, and characterization of the open reading frames which encode novel helicase-related proteins in Saccharomyces cerevisiae by gene disruption and Northern analysis."
Shiratori A., Shibata T., Arisawa M., Hanaoka F., Murakami Y., Eki T.
Yeast 15:219-253(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"A novel mitochondrial DEAD box protein (Mrh4) required for maintenance of mtDNA in Saccharomyces cerevisiae."
Schmidt U., Lehmann K., Stahl U.
FEMS Yeast Res. 2:267-276(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, VARIANTS SER-67 AND SER-438.
Strain: DBY947.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"The proteome of Saccharomyces cerevisiae mitochondria."
Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C.
Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: ATCC 76625 / YPH499.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z72586 Genomic DNA. Translation: CAA96767.1.
BK006941 Genomic DNA. Translation: DAA08038.1.
PIRS64068.
RefSeqNP_011451.1. NM_001180929.1.

3D structure databases

ProteinModelPortalP53166.
SMRP53166. Positions 97-556.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33183. 39 interactions.
DIPDIP-6672N.
IntActP53166. 4 interactions.
MINTMINT-622031.
STRING4932.YGL064C.

Proteomic databases

PaxDbP53166.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGL064C; YGL064C; YGL064C.
GeneID852816.
KEGGsce:YGL064C.

Organism-specific databases

CYGDYGL064c.
SGDS000003032. MRH4.

Phylogenomic databases

eggNOGCOG0513.
GeneTreeENSGT00740000116363.
HOGENOMHOG000065970.
KOK17678.
OMASSARKQE.
OrthoDBEOG79W9DT.

Enzyme and pathway databases

BioCycYEAST:G3O-30570-MONOMER.

Gene expression databases

GenevestigatorP53166.

Family and domain databases

InterProIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
PROSITEPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio972354.

Entry information

Entry nameMRH4_YEAST
AccessionPrimary (citable) accession number: P53166
Secondary accession number(s): D6VU77
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 19, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families