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P53166

- MRH4_YEAST

UniProt

P53166 - MRH4_YEAST

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Protein
ATP-dependent RNA helicase MRH4, mitochondrial
Gene
MRH4, YGL064C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

ATP-binding RNA helicase involved in mitochondrial RNA metabolism. Required for maintenance of mitochondrial DNA.2 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi164 – 1718ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATP-dependent RNA helicase activity Source: SGD
  3. rRNA binding Source: SGD

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. RNA metabolic process Source: SGD
  3. mitochondrial large ribosomal subunit assembly Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30570-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase MRH4, mitochondrial (EC:3.6.4.13)
Alternative name(s):
Mitochondrial RNA helicase 4
Gene namesi
Name:MRH4
Ordered Locus Names:YGL064C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGL064c.
SGDiS000003032. MRH4.

Subcellular locationi

Mitochondrion 3 Publications

GO - Cellular componenti

  1. mitochondrial matrix Source: SGD
  2. mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2626Mitochondrion Reviewed prediction
Add
BLAST
Chaini27 – 561535ATP-dependent RNA helicase MRH4, mitochondrial
PRO_0000041929Add
BLAST

Proteomic databases

MaxQBiP53166.
PaxDbiP53166.

Expressioni

Gene expression databases

GenevestigatoriP53166.

Interactioni

Protein-protein interaction databases

BioGridi33183. 39 interactions.
DIPiDIP-6672N.
IntActiP53166. 4 interactions.
MINTiMINT-622031.
STRINGi4932.YGL064C.

Structurei

3D structure databases

ProteinModelPortaliP53166.
SMRiP53166. Positions 97-556.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini151 – 339189Helicase ATP-binding
Add
BLAST
Domaini370 – 561192Helicase C-terminal
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi117 – 14125Q motif
Add
BLAST
Motifi287 – 2904DEAD box

Domaini

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0513.
GeneTreeiENSGT00740000116363.
HOGENOMiHOG000065970.
KOiK17678.
OMAiSSARKQE.
OrthoDBiEOG79W9DT.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53166-1 [UniParc]FASTAAdd to Basket

« Hide

MSLFFKPVIS PQWSFPVLLK IGVRSYAGGP RTKHKGNSPL ASVPTGSSNK    50
NRKQKAKGKK GNKKNDPDQA FNFGEYGGLK KDVEMNMDST NKLIQKISNF 100
DQLLILPPVR DAVKEIISKE SLKLQDSRKK TSENIIPSPI QTVAIKRISK 150
NLMDPKLQIH AIAAETGSGK TMAYLIPLID YLKRQELETP ELWETLRKNV 200
LIRSIILVPT HELVDQVYET VSKTKTLLGL NSFKWDKATS YRDLLENIKN 250
RIDILVTTPG KLLNLFSIRM ITRPDKVLSK VGFVVLDEAD TLLDRSWLEE 300
THSAIKRIPN INHLIFCSAT IPQEFNKTMQ RLFPTVVPIM TPRLHKLPFA 350
LDFKVINSAL SPFKGSKIKA LAQTLYAISN DDTEPGFEKR CIIFVNEKKN 400
VPEIVNLLNK KFGHNAIGLT GEDTFEERSE KIMPFLSPPR PLSEVVAQST 450
SPPTSLKKFE IPDSNIVIGK LKNTNSNGTA PSNKSLHVLV TTDLMARGLN 500
FKGVRNVVLY DVPKTSIDLI HRVGRTARMK QGGRVFMLTD SKTKSWAKAL 550
PKIIKKHQRL S 561
Length:561
Mass (Da):63,058
Last modified:October 1, 1996 - v1
Checksum:iA8CEE92D461DB6EC
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti67 – 671P → S in strain: DBY947. 1 Publication
Natural varianti438 – 4381P → S in strain: DBY947. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z72586 Genomic DNA. Translation: CAA96767.1.
BK006941 Genomic DNA. Translation: DAA08038.1.
PIRiS64068.
RefSeqiNP_011451.1. NM_001180929.1.

Genome annotation databases

EnsemblFungiiYGL064C; YGL064C; YGL064C.
GeneIDi852816.
KEGGisce:YGL064C.

Cross-referencesi

Web resourcesi

RNA helicases from the baker's yeast Saccharomyces cerevisiae

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z72586 Genomic DNA. Translation: CAA96767.1 .
BK006941 Genomic DNA. Translation: DAA08038.1 .
PIRi S64068.
RefSeqi NP_011451.1. NM_001180929.1.

3D structure databases

ProteinModelPortali P53166.
SMRi P53166. Positions 97-556.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33183. 39 interactions.
DIPi DIP-6672N.
IntActi P53166. 4 interactions.
MINTi MINT-622031.
STRINGi 4932.YGL064C.

Proteomic databases

MaxQBi P53166.
PaxDbi P53166.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGL064C ; YGL064C ; YGL064C .
GeneIDi 852816.
KEGGi sce:YGL064C.

Organism-specific databases

CYGDi YGL064c.
SGDi S000003032. MRH4.

Phylogenomic databases

eggNOGi COG0513.
GeneTreei ENSGT00740000116363.
HOGENOMi HOG000065970.
KOi K17678.
OMAi SSARKQE.
OrthoDBi EOG79W9DT.

Enzyme and pathway databases

BioCyci YEAST:G3O-30570-MONOMER.

Miscellaneous databases

NextBioi 972354.

Gene expression databases

Genevestigatori P53166.

Family and domain databases

Gene3Di 3.40.50.300. 3 hits.
InterProi IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view ]
SMARTi SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
PROSITEi PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The characterization of two new clusters of duplicated genes suggests a 'Lego' organization of the yeast Saccharomyces cerevisiae chromosomes."
    Feuermann M., de Montigny J., Potier S., Souciet J.-L.
    Yeast 13:861-869(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Systematic identification, classification, and characterization of the open reading frames which encode novel helicase-related proteins in Saccharomyces cerevisiae by gene disruption and Northern analysis."
    Shiratori A., Shibata T., Arisawa M., Hanaoka F., Murakami Y., Eki T.
    Yeast 15:219-253(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "A novel mitochondrial DEAD box protein (Mrh4) required for maintenance of mtDNA in Saccharomyces cerevisiae."
    Schmidt U., Lehmann K., Stahl U.
    FEMS Yeast Res. 2:267-276(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, VARIANTS SER-67 AND SER-438.
    Strain: DBY947.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.

Entry informationi

Entry nameiMRH4_YEAST
AccessioniPrimary (citable) accession number: P53166
Secondary accession number(s): D6VU77
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 736 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

Similar proteinsi