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P53166

- MRH4_YEAST

UniProt

P53166 - MRH4_YEAST

Protein

ATP-dependent RNA helicase MRH4, mitochondrial

Gene

MRH4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    ATP-binding RNA helicase involved in mitochondrial RNA metabolism. Required for maintenance of mitochondrial DNA.2 Publications

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi164 – 1718ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ATP-dependent RNA helicase activity Source: SGD
    3. mitochondrial ribosomal large subunit rRNA binding Source: SGD

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. mitochondrial large ribosomal subunit assembly Source: SGD
    3. RNA metabolic process Source: SGD

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30570-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent RNA helicase MRH4, mitochondrial (EC:3.6.4.13)
    Alternative name(s):
    Mitochondrial RNA helicase 4
    Gene namesi
    Name:MRH4
    Ordered Locus Names:YGL064C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGL064c.
    SGDiS000003032. MRH4.

    Subcellular locationi

    Mitochondrion 3 Publications

    GO - Cellular componenti

    1. mitochondrial matrix Source: SGD
    2. mitochondrion Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2626MitochondrionSequence AnalysisAdd
    BLAST
    Chaini27 – 561535ATP-dependent RNA helicase MRH4, mitochondrialPRO_0000041929Add
    BLAST

    Proteomic databases

    MaxQBiP53166.
    PaxDbiP53166.

    Expressioni

    Gene expression databases

    GenevestigatoriP53166.

    Interactioni

    Protein-protein interaction databases

    BioGridi33183. 39 interactions.
    DIPiDIP-6672N.
    IntActiP53166. 4 interactions.
    MINTiMINT-622031.
    STRINGi4932.YGL064C.

    Structurei

    3D structure databases

    ProteinModelPortaliP53166.
    SMRiP53166. Positions 97-556.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini151 – 339189Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini370 – 561192Helicase C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi117 – 14125Q motifAdd
    BLAST
    Motifi287 – 2904DEAD box

    Domaini

    The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

    Sequence similaritiesi

    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0513.
    GeneTreeiENSGT00740000116363.
    HOGENOMiHOG000065970.
    KOiK17678.
    OMAiSSARKQE.
    OrthoDBiEOG79W9DT.

    Family and domain databases

    Gene3Di3.40.50.300. 3 hits.
    InterProiIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00270. DEAD. 1 hit.
    PF00271. Helicase_C. 1 hit.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P53166-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLFFKPVIS PQWSFPVLLK IGVRSYAGGP RTKHKGNSPL ASVPTGSSNK    50
    NRKQKAKGKK GNKKNDPDQA FNFGEYGGLK KDVEMNMDST NKLIQKISNF 100
    DQLLILPPVR DAVKEIISKE SLKLQDSRKK TSENIIPSPI QTVAIKRISK 150
    NLMDPKLQIH AIAAETGSGK TMAYLIPLID YLKRQELETP ELWETLRKNV 200
    LIRSIILVPT HELVDQVYET VSKTKTLLGL NSFKWDKATS YRDLLENIKN 250
    RIDILVTTPG KLLNLFSIRM ITRPDKVLSK VGFVVLDEAD TLLDRSWLEE 300
    THSAIKRIPN INHLIFCSAT IPQEFNKTMQ RLFPTVVPIM TPRLHKLPFA 350
    LDFKVINSAL SPFKGSKIKA LAQTLYAISN DDTEPGFEKR CIIFVNEKKN 400
    VPEIVNLLNK KFGHNAIGLT GEDTFEERSE KIMPFLSPPR PLSEVVAQST 450
    SPPTSLKKFE IPDSNIVIGK LKNTNSNGTA PSNKSLHVLV TTDLMARGLN 500
    FKGVRNVVLY DVPKTSIDLI HRVGRTARMK QGGRVFMLTD SKTKSWAKAL 550
    PKIIKKHQRL S 561
    Length:561
    Mass (Da):63,058
    Last modified:October 1, 1996 - v1
    Checksum:iA8CEE92D461DB6EC
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti67 – 671P → S in strain: DBY947. 1 Publication
    Natural varianti438 – 4381P → S in strain: DBY947. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72586 Genomic DNA. Translation: CAA96767.1.
    BK006941 Genomic DNA. Translation: DAA08038.1.
    PIRiS64068.
    RefSeqiNP_011451.1. NM_001180929.1.

    Genome annotation databases

    EnsemblFungiiYGL064C; YGL064C; YGL064C.
    GeneIDi852816.
    KEGGisce:YGL064C.

    Cross-referencesi

    Web resourcesi

    RNA helicases from the baker's yeast Saccharomyces cerevisiae

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72586 Genomic DNA. Translation: CAA96767.1 .
    BK006941 Genomic DNA. Translation: DAA08038.1 .
    PIRi S64068.
    RefSeqi NP_011451.1. NM_001180929.1.

    3D structure databases

    ProteinModelPortali P53166.
    SMRi P53166. Positions 97-556.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33183. 39 interactions.
    DIPi DIP-6672N.
    IntActi P53166. 4 interactions.
    MINTi MINT-622031.
    STRINGi 4932.YGL064C.

    Proteomic databases

    MaxQBi P53166.
    PaxDbi P53166.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL064C ; YGL064C ; YGL064C .
    GeneIDi 852816.
    KEGGi sce:YGL064C.

    Organism-specific databases

    CYGDi YGL064c.
    SGDi S000003032. MRH4.

    Phylogenomic databases

    eggNOGi COG0513.
    GeneTreei ENSGT00740000116363.
    HOGENOMi HOG000065970.
    KOi K17678.
    OMAi SSARKQE.
    OrthoDBi EOG79W9DT.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30570-MONOMER.

    Miscellaneous databases

    NextBioi 972354.

    Gene expression databases

    Genevestigatori P53166.

    Family and domain databases

    Gene3Di 3.40.50.300. 3 hits.
    InterProi IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00270. DEAD. 1 hit.
    PF00271. Helicase_C. 1 hit.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    PROSITEi PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The characterization of two new clusters of duplicated genes suggests a 'Lego' organization of the yeast Saccharomyces cerevisiae chromosomes."
      Feuermann M., de Montigny J., Potier S., Souciet J.-L.
      Yeast 13:861-869(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Systematic identification, classification, and characterization of the open reading frames which encode novel helicase-related proteins in Saccharomyces cerevisiae by gene disruption and Northern analysis."
      Shiratori A., Shibata T., Arisawa M., Hanaoka F., Murakami Y., Eki T.
      Yeast 15:219-253(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "A novel mitochondrial DEAD box protein (Mrh4) required for maintenance of mtDNA in Saccharomyces cerevisiae."
      Schmidt U., Lehmann K., Stahl U.
      FEMS Yeast Res. 2:267-276(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, VARIANTS SER-67 AND SER-438.
      Strain: DBY947.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: ATCC 76625 / YPH499.

    Entry informationi

    Entry nameiMRH4_YEAST
    AccessioniPrimary (citable) accession number: P53166
    Secondary accession number(s): D6VU77
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 128 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 736 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3