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Protein

ATP-dependent RNA helicase MRH4, mitochondrial

Gene

MRH4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP-binding RNA helicase involved in mitochondrial RNA metabolism. Required for maintenance of mitochondrial DNA.2 Publications

Miscellaneous

Present with 736 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi164 – 171ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: SGD
  • mitochondrial ribosomal large subunit rRNA binding Source: SGD

GO - Biological processi

  • mitochondrial large ribosomal subunit assembly Source: SGD
  • RNA metabolic process Source: SGD
  • RNA secondary structure unwinding Source: GO_Central

Keywordsi

Molecular functionHelicase, Hydrolase, RNA-binding
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30570-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase MRH4, mitochondrial (EC:3.6.4.13)
Alternative name(s):
Mitochondrial RNA helicase 4
Gene namesi
Name:MRH4
Ordered Locus Names:YGL064C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL064C
SGDiS000003032 MRH4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 26MitochondrionSequence analysisAdd BLAST26
ChainiPRO_000004192927 – 561ATP-dependent RNA helicase MRH4, mitochondrialAdd BLAST535

Proteomic databases

MaxQBiP53166
PaxDbiP53166
PRIDEiP53166

PTM databases

iPTMnetiP53166

Interactioni

Protein-protein interaction databases

BioGridi33183, 91 interactors
DIPiDIP-6672N
IntActiP53166, 4 interactors
MINTiP53166
STRINGi4932.YGL064C

Structurei

3D structure databases

ProteinModelPortaliP53166
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini151 – 339Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST189
Domaini370 – 561Helicase C-terminalPROSITE-ProRule annotationAdd BLAST192

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi117 – 141Q motifAdd BLAST25
Motifi287 – 290DEAD box4

Domaini

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000065970
InParanoidiP53166
KOiK17678
OMAiWSIGKAG
OrthoDBiEOG092C2Z1Z

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53166-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLFFKPVIS PQWSFPVLLK IGVRSYAGGP RTKHKGNSPL ASVPTGSSNK
60 70 80 90 100
NRKQKAKGKK GNKKNDPDQA FNFGEYGGLK KDVEMNMDST NKLIQKISNF
110 120 130 140 150
DQLLILPPVR DAVKEIISKE SLKLQDSRKK TSENIIPSPI QTVAIKRISK
160 170 180 190 200
NLMDPKLQIH AIAAETGSGK TMAYLIPLID YLKRQELETP ELWETLRKNV
210 220 230 240 250
LIRSIILVPT HELVDQVYET VSKTKTLLGL NSFKWDKATS YRDLLENIKN
260 270 280 290 300
RIDILVTTPG KLLNLFSIRM ITRPDKVLSK VGFVVLDEAD TLLDRSWLEE
310 320 330 340 350
THSAIKRIPN INHLIFCSAT IPQEFNKTMQ RLFPTVVPIM TPRLHKLPFA
360 370 380 390 400
LDFKVINSAL SPFKGSKIKA LAQTLYAISN DDTEPGFEKR CIIFVNEKKN
410 420 430 440 450
VPEIVNLLNK KFGHNAIGLT GEDTFEERSE KIMPFLSPPR PLSEVVAQST
460 470 480 490 500
SPPTSLKKFE IPDSNIVIGK LKNTNSNGTA PSNKSLHVLV TTDLMARGLN
510 520 530 540 550
FKGVRNVVLY DVPKTSIDLI HRVGRTARMK QGGRVFMLTD SKTKSWAKAL
560
PKIIKKHQRL S
Length:561
Mass (Da):63,058
Last modified:October 1, 1996 - v1
Checksum:iA8CEE92D461DB6EC
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti67P → S in strain: DBY947. 1 Publication1
Natural varianti438P → S in strain: DBY947. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72586 Genomic DNA Translation: CAA96767.1
BK006941 Genomic DNA Translation: DAA08038.1
PIRiS64068
RefSeqiNP_011451.1, NM_001180929.1

Genome annotation databases

EnsemblFungiiYGL064C; YGL064C; YGL064C
GeneIDi852816
KEGGisce:YGL064C

Similar proteinsi

Entry informationi

Entry nameiMRH4_YEAST
AccessioniPrimary (citable) accession number: P53166
Secondary accession number(s): D6VU77
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 20, 2018
This is version 162 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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