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P53165

- SGF73_YEAST

UniProt

P53165 - SGF73_YEAST

Protein

SAGA-associated factor 73

Gene

SGF73

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Functions as component of the transcription regulatory histone acetylation (HAT) complex SAGA. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs).

    GO - Molecular functioni

    1. enzyme activator activity Source: SGD
    2. protein binding Source: IntAct
    3. structural molecule activity Source: SGD

    GO - Biological processi

    1. chromatin modification Source: SGD
    2. histone acetylation Source: SGD
    3. histone deubiquitination Source: SGD
    4. mRNA export from nucleus Source: SGD
    5. positive regulation of catalytic activity Source: GOC
    6. positive regulation of RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
    7. protein complex assembly Source: SGD
    8. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30571-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    SAGA-associated factor 73
    Alternative name(s):
    73 kDa SAGA-associated factor
    SAGA histone acetyltransferase complex 73 kDa subunit
    Gene namesi
    Name:SGF73
    Ordered Locus Names:YGL066W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGL066w.
    SGDiS000003034. SGF73.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. cytosol Source: SGD
    2. DUBm complex Source: SGD
    3. nucleus Source: SGD
    4. SAGA complex Source: SGD
    5. SLIK (SAGA-like) complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 657657SAGA-associated factor 73PRO_0000202764Add
    BLAST

    Proteomic databases

    MaxQBiP53165.
    PaxDbiP53165.
    PeptideAtlasiP53165.

    Expressioni

    Gene expression databases

    GenevestigatoriP53165.

    Interactioni

    Subunit structurei

    Component of the 1.8 MDa SAGA complex, which consists of at least of TRA1, CHD1, SPT7, TAF5, ADA3, SGF73, SPT20/ADA5, SPT8, TAF12, TAF6, HFI1/ADA1, UBP8, GCN5, ADA2, SPT3, SGF29, TAF10, TAF9, SGF11 and SUS1. TAF5, TAF6, TAF9, TAF19, TAF12 and ADA1 seem to be present in 2 copies. SAGA is built of 5 distinct domains with specialized functions. Domain I (containing TRA1) probably represents the activator interaction surface. Domain II (containing TAF5 and TAF6, and probably TAF9 and TAF10), domain III (containing GCN5, TAF10, SPT7, TAF5 and ADA1, and probably ADA2, ADA3 and TAF12), and domain IV (containing HFI1/ADA1 and TAF6, and probably TAF9) are believed to play primarily an architectural role. Domain III also harbors the HAT activity. Domain V (containing SPT3 and SPT20, and probably SPT8) represents the TBP-interacting module, which may be associated transiently with SAGA.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SPT20P508755EBI-23812,EBI-17751

    Protein-protein interaction databases

    BioGridi33181. 355 interactions.
    DIPiDIP-5211N.
    IntActiP53165. 92 interactions.
    MINTiMINT-548904.
    STRINGi4932.YGL066W.

    Structurei

    Secondary structure

    1
    657
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi7 – 115
    Helixi13 – 186
    Turni19 – 224
    Beta strandi28 – 303
    Helixi32 – 354
    Helixi36 – 416
    Beta strandi47 – 493
    Helixi51 – 577
    Beta strandi70 – 723
    Beta strandi76 – 783
    Turni79 – 813
    Beta strandi84 – 863
    Helixi87 – 893
    Turni90 – 923

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2LO3NMR-A59-102[»]
    3M99X-ray2.70D1-104[»]
    3MHHX-ray2.45E1-96[»]
    3MHSX-ray1.89E1-96[»]
    4FIPX-ray2.69D/H1-96[»]
    4FJCX-ray2.83D/H1-96[»]
    4FK5X-ray2.03E1-96[»]
    ProteinModelPortaliP53165.
    SMRiP53165. Positions 5-96.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP53165.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini220 – 28667SCA7PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi141 – 15010Poly-Asp
    Compositional biasi153 – 16210Poly-Asp
    Compositional biasi466 – 47611Poly-GlnAdd
    BLAST
    Compositional biasi517 – 52610Poly-Gln

    Sequence similaritiesi

    Belongs to the ataxin-7 family.Curated
    Contains 1 SCA7 domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG285209.
    HOGENOMiHOG000142039.
    KOiK11365.
    OMAiNINEIGH.
    OrthoDBiEOG7NSBB2.

    Family and domain databases

    InterProiIPR013243. SCA7_dom.
    [Graphical view]
    PfamiPF08313. SCA7. 1 hit.
    [Graphical view]
    PROSITEiPS51505. SCA7. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53165-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRSGDAEIKG IKPKVIEEYS LSQGSGPSND SWKSLMSSAK DTPLQYDHMN    50
    RESLKKYFNP NAQLIEDPLD KPIQYRVCEK CGKPLALTAI VDHLENHCAG 100
    ASGKSSTDPR DESTRETIRN GVESTGRNNN DDDNSNDNNN DDDDDDDNDD 150
    NEDDDDADDD DDNSNGANYK KNDSSFNPLK RSTSMESANT PNMDTKRSKT 200
    GTPQTFSSSI KKQKKVKQRN PTEKHLIDFN KQCGVELPEG GYCARSLTCK 250
    SHSMGAKRAV SGRSKPYDVL LADYHREHQT KIGAAAEKRA KQQELQKLQK 300
    QIQKEQKKHT QQQKQGQRSK QRNVNGGKSA KNGGKSTVHN GNNINEIGHV 350
    NLTPEEETTQ VLNGVSRSFP LPLESTVLSS VRYRTKYFRM REMFASSFSV 400
    KPGYTSPGYG AIHSRVGCLD LDRTTDYKFR VRTPQPINHL TNQNLNPKQI 450
    QRLQQQRALQ AQLLSQQQQQ QQQQQQHHSP QAQAQASTQQ PTQGMVPNHF 500
    PGGATNSSFN ANVSSKQIQQ QQQQQQHKSQ DTGLTPLEIQ SQQQKLRQQQ 550
    LQQQKFEAAA SYLANATKLM QESNQDSHLS GTHNNNSSKN GNNNLMTMKA 600
    SISSPNTSVN SIQSPPSVNS VNGSGQGVST GINVSGNNGR IEVGIGNSVN 650
    PYNGRIN 657
    Length:657
    Mass (Da):72,878
    Last modified:October 1, 1996 - v1
    Checksum:i4618E2E47824321D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72588 Genomic DNA. Translation: CAA96770.1.
    Z72587 Genomic DNA. Translation: CAA96769.1.
    BK006941 Genomic DNA. Translation: DAA08036.1.
    PIRiS64073.
    RefSeqiNP_011449.1. NM_001180931.1.

    Genome annotation databases

    EnsemblFungiiYGL066W; YGL066W; YGL066W.
    GeneIDi852814.
    KEGGisce:YGL066W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72588 Genomic DNA. Translation: CAA96770.1 .
    Z72587 Genomic DNA. Translation: CAA96769.1 .
    BK006941 Genomic DNA. Translation: DAA08036.1 .
    PIRi S64073.
    RefSeqi NP_011449.1. NM_001180931.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2LO3 NMR - A 59-102 [» ]
    3M99 X-ray 2.70 D 1-104 [» ]
    3MHH X-ray 2.45 E 1-96 [» ]
    3MHS X-ray 1.89 E 1-96 [» ]
    4FIP X-ray 2.69 D/H 1-96 [» ]
    4FJC X-ray 2.83 D/H 1-96 [» ]
    4FK5 X-ray 2.03 E 1-96 [» ]
    ProteinModelPortali P53165.
    SMRi P53165. Positions 5-96.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33181. 355 interactions.
    DIPi DIP-5211N.
    IntActi P53165. 92 interactions.
    MINTi MINT-548904.
    STRINGi 4932.YGL066W.

    Proteomic databases

    MaxQBi P53165.
    PaxDbi P53165.
    PeptideAtlasi P53165.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL066W ; YGL066W ; YGL066W .
    GeneIDi 852814.
    KEGGi sce:YGL066W.

    Organism-specific databases

    CYGDi YGL066w.
    SGDi S000003034. SGF73.

    Phylogenomic databases

    eggNOGi NOG285209.
    HOGENOMi HOG000142039.
    KOi K11365.
    OMAi NINEIGH.
    OrthoDBi EOG7NSBB2.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30571-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P53165.
    NextBioi 972348.

    Gene expression databases

    Genevestigatori P53165.

    Family and domain databases

    InterProi IPR013243. SCA7_dom.
    [Graphical view ]
    Pfami PF08313. SCA7. 1 hit.
    [Graphical view ]
    PROSITEi PS51505. SCA7. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
      Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
      Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "The characterization of two new clusters of duplicated genes suggests a 'Lego' organization of the yeast Saccharomyces cerevisiae chromosomes."
      Feuermann M., de Montigny J., Potier S., Souciet J.-L.
      Yeast 13:861-869(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 115-657.
      Strain: ATCC 204508 / S288c.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Proteomics of the eukaryotic transcription machinery: identification of proteins associated with components of yeast TFIID by multidimensional mass spectrometry."
      Sanders S.L., Jennings J., Canutescu A., Link A.J., Weil P.A.
      Mol. Cell. Biol. 22:4723-4738(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE SAGA COMPLEX.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Molecular architecture of the S. cerevisiae SAGA complex."
      Wu P.Y., Ruhlmann C., Winston F., Schultz P.
      Mol. Cell 15:199-208(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING OF THE SAGA COMPLEX.

    Entry informationi

    Entry nameiSGF73_YEAST
    AccessioniPrimary (citable) accession number: P53165
    Secondary accession number(s): D6VU75
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 486 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3