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Protein

NADH pyrophosphatase

Gene

NPY1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 846 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

NAD(H) + H2O = AMP + NMN(H).

Cofactori

Mg2+, Mn2+Note: Divalent metal cations. Mg2+ or Mn2+.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi272Divalent metal cationBy similarity1
Metal bindingi276Divalent metal cationBy similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • NAD+ diphosphatase activity Source: SGD

GO - Biological processi

  • NADH metabolic process Source: SGD

Keywordsi

Molecular functionHydrolase
LigandMagnesium, Manganese, Metal-binding, NAD

Enzyme and pathway databases

BioCyciYEAST:YGL067W-MONOMER.
BRENDAi3.6.1.22. 984.
ReactomeiR-SCE-197264. Nicotinamide salvaging.
R-SCE-499943. Interconversion of nucleotide di- and triphosphates.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH pyrophosphatase (EC:3.6.1.22)
Gene namesi
Name:NPY1
Ordered Locus Names:YGL067W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL067W.
SGDiS000003035. NPY1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000569611 – 384NADH pyrophosphataseAdd BLAST384

Proteomic databases

MaxQBiP53164.
PRIDEiP53164.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi33180. 93 interactors.
IntActiP53164. 1 interactor.
MINTiMINT-2781196.
STRINGi4932.YGL067W.

Structurei

3D structure databases

ProteinModelPortaliP53164.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini219 – 351Nudix hydrolasePROSITE-ProRule annotationAdd BLAST133

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi257 – 278Nudix boxAdd BLAST22

Sequence similaritiesi

Belongs to the Nudix hydrolase family. NudC subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00530000063600.
HOGENOMiHOG000247937.
InParanoidiP53164.
KOiK03426.
OMAiTIAGFME.
OrthoDBiEOG092C43U1.

Family and domain databases

InterProiView protein in InterPro
IPR015797. NUDIX_hydrolase-like_dom_sf.
IPR020084. NUDIX_hydrolase_CS.
IPR000086. NUDIX_hydrolase_dom.
IPR015376. Znr_NADH_PPase.
PfamiView protein in Pfam
PF00293. NUDIX. 1 hit.
PF09297. zf-NADH-PPase. 1 hit.
SUPFAMiSSF55811. SSF55811. 1 hit.
PROSITEiView protein in PROSITE
PS51462. NUDIX. 1 hit.
PS00893. NUDIX_BOX. 1 hit.

Sequencei

Sequence statusi: Complete.

P53164-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTAVTFFGQ HVLNRVSFLR CSKEFIKKSL NHDSTVFIPF IEGEALISPE
60 70 80 90 100
NGDLVQLSNS VKSYKNILSA IVPLYTTLLN TTRSRSDESG INVTFLGLLE
110 120 130 140 150
GTDSAFNFEW SNISYKGTPY FGLDIRVTES TLFKKVDFEP IFSYPKVTRD
160 170 180 190 200
HIFKQTNEDA SLYSQGKMYL DWLAKYKFCP GCGSPLFPVE AGTKLQCSNE
210 220 230 240 250
NRNVYCNVRD ARINNVCFPR TDPTVIIALT NSDYSKCCLA RSKKRYGDFV
260 270 280 290 300
LYSTIAGFME PSETIEEACI REIWEETGIS CKNIDIVRSQ PWPYPCSLMI
310 320 330 340 350
GCLGIVQFNS KNEVINLNHD DELLDAQWFD TTEIIQALDK YAGGYRVPFK
360 370 380
NDINLPGSTT IAFQLINHVC ENYKNLRKTS SSHL
Length:384
Mass (Da):43,516
Last modified:October 1, 1996 - v1
Checksum:i96D6768CE2BC8F0B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti44E → D in AAT93207 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72589 Genomic DNA. Translation: CAA96771.1.
AY693188 Genomic DNA. Translation: AAT93207.1.
BK006941 Genomic DNA. Translation: DAA08035.1.
PIRiS64074.
RefSeqiNP_011448.3. NM_001180932.3.

Genome annotation databases

EnsemblFungiiYGL067W; YGL067W; YGL067W.
GeneIDi852813.
KEGGisce:YGL067W.

Similar proteinsi

Entry informationi

Entry nameiNPY1_YEAST
AccessioniPrimary (citable) accession number: P53164
Secondary accession number(s): D6VU74, E9P928
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 22, 2017
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names