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P53163 (MNP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
54S ribosomal protein L12, mitochondrial
Alternative name(s):
Mitochondrial-nucleoid protein 1
Gene names
Name:MNP1
Synonyms:MRPL12
Ordered Locus Names:YGL068W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length194 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Component of the mitochondrial large ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S). Ref.4 Ref.8

Subcellular location

Mitochondrion Ref.4 Ref.5 Ref.7.

Post-translational modification

N-glycosylated. HAMAP-Rule MF_00368

Miscellaneous

Present with 6900 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the ribosomal protein L7/L12P family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3333Mitochondrion Ref.4
Chain34 – 19416154S ribosomal protein L12, mitochondrial HAMAP-Rule MF_00368
PRO_0000030460

Amino acid modifications

Glycosylation341N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict341N → D AA sequence Ref.4

Sequences

Sequence LengthMass (Da)Tools
P53163 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: D7892681B778B8A9

FASTA19420,650
        10         20         30         40         50         60 
MSLRILAKRS SSIWMKTRVT PALISPITIT TRFNSTTTTA PSHKDDVRPV DPKISKIVQD 

        70         80         90        100        110        120 
ISQLTLLETS SLINELKTVL NIPEISMPMG GFMAGAAGAG AGNVPSSTGE AGSGAEEEAK 

       130        140        150        160        170        180 
PEAKTVFTVK LDSFDTKTKA KVIKEVKGLL GLSLVEAKKF VEAAPKVLKE NVAKDDAEKI 

       190 
KKTLEDLGAK VSLE 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"A 22 kDa protein specific for yeast mitochondrial nucleoids is an unidentified putative ribosomal protein encoded in open reading frame YGL068W."
Sato H., Miyakawa I.
Protoplasma 223:175-182(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 34-48, SUBUNIT, SUBCELLULAR LOCATION.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"The proteome of Saccharomyces cerevisiae mitochondria."
Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C.
Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: ATCC 76625 / YPH499.
[8]"Identification and comparative analysis of the large subunit mitochondrial ribosomal proteins of Neurospora crassa."
Gan X., Arita K., Isono S., Kitakawa M., Yoshino K., Yonezawa K., Kato A., Inoue H., Isono K.
FEMS Microbiol. Lett. 254:157-164(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT.
[9]"Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals new roles for protein glycosylation in eukaryotes."
Kung L.A., Tao S.-C., Qian J., Smith M.G., Snyder M., Zhu H.
Mol. Syst. Biol. 5:308-308(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z72591 Genomic DNA. Translation: CAA96773.1.
BK006941 Genomic DNA. Translation: DAA08034.1.
PIRS64075.
RefSeqNP_011447.1. NM_001180933.1.

3D structure databases

ProteinModelPortalP53163.
SMRP53163. Positions 139-194.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33179. 27 interactions.
DIPDIP-6428N.
IntActP53163. 4 interactions.
MINTMINT-705819.
STRING4932.YGL068W.

Proteomic databases

MaxQBP53163.
PaxDbP53163.
PeptideAtlasP53163.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGL068W; YGL068W; YGL068W.
GeneID852811.
KEGGsce:YGL068W.

Organism-specific databases

CYGDYGL068w.
SGDS000003036. MNP1.

Phylogenomic databases

eggNOGCOG0222.
GeneTreeENSGT00390000000190.
HOGENOMHOG000248814.
KOK02935.
OMAMYCTRYC.
OrthoDBEOG70GMV4.

Enzyme and pathway databases

BioCycYEAST:G3O-30572-MONOMER.

Gene expression databases

GenevestigatorP53163.

Family and domain databases

Gene3D3.30.1390.10. 1 hit.
HAMAPMF_00368. Ribosomal_L7_L12.
InterProIPR000206. Ribosomal_L7/12.
IPR014719. Ribosomal_L7/12_C/ClpS-like.
IPR013823. Ribosomal_L7/L12_C.
IPR008932. Ribosomal_L7/L12_oligo.
[Graphical view]
PANTHERPTHR11809. PTHR11809. 1 hit.
PfamPF00542. Ribosomal_L12. 1 hit.
[Graphical view]
ProDomPD001326. Ribosomal_L7/L12_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF48300. SSF48300. 1 hit.
SSF54736. SSF54736. 1 hit.
ProtoNetSearch...

Other

NextBio972342.
PROP53163.

Entry information

Entry nameMNP1_YEAST
AccessionPrimary (citable) accession number: P53163
Secondary accession number(s): D6VU73
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries