P53163 (MNP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
54S ribosomal protein L12, mitochondrial

Alternative name(s):
Mitochondrial-nucleoid protein 1

Gene names

Name:	MNP1
Synonyms:	MRPL12
Ordered Locus Names:	YGL068W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	194 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the mitochondrial large ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S). Ref.4 Ref.8
Subcellular location	Mitochondrion Ref.4 Ref.5 Ref.7.
Post-translational modification	N-glycosylated. HAMAP-Rule MF_00368
Miscellaneous	Present with 6900 molecules/cell in log phase SD medium. HAMAP-Rule MF_00368
Sequence similarities	Belongs to the ribosomal protein L7/L12P family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	aerobic respiration Inferred from mutant phenotype Ref.4. Source: SGD translation Inferred from electronic annotation. Source: InterPro
Cellular_component	mitochondrial nucleoid Inferred from direct assay Ref.4. Source: SGD ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	structural constituent of ribosome Inferred from sequence or structural similarity PubMed 9445368. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 33	33	Mitochondrion Ref.4
	Chain	34 – 194	161	54S ribosomal protein L12, mitochondrial HAMAP-Rule MF_00368		PRO_0000030460
Amino acid modifications
	Glycosylation	34	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	34	1	N → D AA sequence Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P53163 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: D7892681B778B8A9	FASTA	194	20,650
	10 20 30 40 50 60 MSLRILAKRS SSIWMKTRVT PALISPITIT TRFNSTTTTA PSHKDDVRPV DPKISKIVQD 70 80 90 100 110 120 ISQLTLLETS SLINELKTVL NIPEISMPMG GFMAGAAGAG AGNVPSSTGE AGSGAEEEAK 130 140 150 160 170 180 PEAKTVFTVK LDSFDTKTKA KVIKEVKGLL GLSLVEAKKF VEAAPKVLKE NVAKDDAEKI 190 KKTLEDLGAK VSLE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII." Rieger M., Brueckner M., Schaefer M., Mueller-Auer S. Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"A 22 kDa protein specific for yeast mitochondrial nucleoids is an unidentified putative ribosomal protein encoded in open reading frame YGL068W." Sato H., Miyakawa I. Protoplasma 223:175-182(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 34-48, SUBUNIT, SUBCELLULAR LOCATION.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
[8]	"Identification and comparative analysis of the large subunit mitochondrial ribosomal proteins of Neurospora crassa." Gan X., Arita K., Isono S., Kitakawa M., Yoshino K., Yonezawa K., Kato A., Inoue H., Isono K. FEMS Microbiol. Lett. 254:157-164(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT.
[9]	"Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals new roles for protein glycosylation in eukaryotes." Kung L.A., Tao S.-C., Qian J., Smith M.G., Snyder M., Zhu H. Mol. Syst. Biol. 5:308-308(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z72591 Genomic DNA. Translation: CAA96773.1. BK006941 Genomic DNA. Translation: DAA08034.1.
PIR	S64075.
RefSeq	NP_011447.1. NM_001180933.1.
3D structure databases
ProteinModelPortal	P53163.
SMR	P53163. Positions 139-194.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6428N.
IntAct	P53163. 6 interactions.
MINT	MINT-705819.
STRING	4932.YGL068W.
Proteomic databases
PaxDb	P53163.
PeptideAtlas	P53163.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL068W; YGL068W; YGL068W.
GeneID	852811.
KEGG	sce:YGL068W.
Organism-specific databases
CYGD	YGL068w.
SGD	S000003036. MNP1.
Phylogenomic databases
eggNOG	COG0222.
GeneTree	ENSGT00390000000190.
HOGENOM	HOG000248814.
KO	K02935.
OMA	GVAKPPN.
OrthoDB	EOG49CTJ7.
Gene expression databases
Genevestigator	P53163.
GermOnline	YGL068W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.30.1390.10. 1 hit.
HAMAP	MF_00368. Ribosomal_L7_L12.
InterPro	IPR015607. Ribosomal_L12_mit. IPR014719. Ribosomal_L7/12_C/ClpS-like. IPR013823. Ribosomal_L7/L12_C. IPR008932. Ribosomal_L7/L12_oligo. [Graphical view]
PANTHER	PTHR11809:SF2. PTHR11809:SF2. 1 hit.
Pfam	PF00542. Ribosomal_L12. 1 hit. [Graphical view]
ProDom	PD001326. Ribosomal_L7/L12_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF48300. Ribosomal_L12/7. 1 hit. SSF54736. Ribosomal_L7/12_C/ClpS-like. 1 hit.
ProtoNet	Search...
Other
NextBio	972342.

Entry information

Entry name

MNP1_YEAST

Accession

Primary (citable) accession number: P53163
Secondary accession number(s): D6VU73

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program