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P53163

- MNP1_YEAST

UniProt

P53163 - MNP1_YEAST

Protein

54S ribosomal protein L12, mitochondrial

Gene

MNP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    GO - Molecular functioni

    1. structural constituent of ribosome Source: SGD

    GO - Biological processi

    1. aerobic respiration Source: SGD
    2. translation Source: InterPro

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30572-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    54S ribosomal protein L12, mitochondrial
    Alternative name(s):
    Mitochondrial-nucleoid protein 1
    Gene namesi
    Name:MNP1
    Synonyms:MRPL12
    Ordered Locus Names:YGL068W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGL068w.
    SGDiS000003036. MNP1.

    Subcellular locationi

    Mitochondrion 4 Publications

    GO - Cellular componenti

    1. mitochondrial nucleoid Source: SGD
    2. ribosome Source: UniProtKB-KW

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3333Mitochondrion1 PublicationAdd
    BLAST
    Chaini34 – 19416154S ribosomal protein L12, mitochondrialPRO_0000030460Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi34 – 341N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    N-glycosylated.1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiP53163.
    PaxDbiP53163.
    PeptideAtlasiP53163.

    Expressioni

    Gene expression databases

    GenevestigatoriP53163.

    Interactioni

    Subunit structurei

    Component of the mitochondrial large ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S).2 Publications

    Protein-protein interaction databases

    BioGridi33179. 27 interactions.
    DIPiDIP-6428N.
    IntActiP53163. 4 interactions.
    MINTiMINT-705819.
    STRINGi4932.YGL068W.

    Structurei

    3D structure databases

    ProteinModelPortaliP53163.
    SMRiP53163. Positions 139-194.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein L7/L12P family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0222.
    GeneTreeiENSGT00390000000190.
    HOGENOMiHOG000248814.
    KOiK02935.
    OMAiMYCTRYC.
    OrthoDBiEOG70GMV4.

    Family and domain databases

    Gene3Di3.30.1390.10. 1 hit.
    HAMAPiMF_00368. Ribosomal_L7_L12.
    InterProiIPR000206. Ribosomal_L7/12.
    IPR014719. Ribosomal_L7/12_C/ClpS-like.
    IPR013823. Ribosomal_L7/L12_C.
    IPR008932. Ribosomal_L7/L12_oligo.
    [Graphical view]
    PANTHERiPTHR11809. PTHR11809. 1 hit.
    PfamiPF00542. Ribosomal_L12. 1 hit.
    [Graphical view]
    ProDomiPD001326. Ribosomal_L7/L12_C. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF48300. SSF48300. 1 hit.
    SSF54736. SSF54736. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P53163-1 [UniParc]FASTAAdd to Basket

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    MSLRILAKRS SSIWMKTRVT PALISPITIT TRFNSTTTTA PSHKDDVRPV    50
    DPKISKIVQD ISQLTLLETS SLINELKTVL NIPEISMPMG GFMAGAAGAG 100
    AGNVPSSTGE AGSGAEEEAK PEAKTVFTVK LDSFDTKTKA KVIKEVKGLL 150
    GLSLVEAKKF VEAAPKVLKE NVAKDDAEKI KKTLEDLGAK VSLE 194
    Length:194
    Mass (Da):20,650
    Last modified:October 1, 1996 - v1
    Checksum:iD7892681B778B8A9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti34 – 341N → D AA sequence (PubMed:15221522)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72591 Genomic DNA. Translation: CAA96773.1.
    BK006941 Genomic DNA. Translation: DAA08034.1.
    PIRiS64075.
    RefSeqiNP_011447.1. NM_001180933.1.

    Genome annotation databases

    EnsemblFungiiYGL068W; YGL068W; YGL068W.
    GeneIDi852811.
    KEGGisce:YGL068W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72591 Genomic DNA. Translation: CAA96773.1 .
    BK006941 Genomic DNA. Translation: DAA08034.1 .
    PIRi S64075.
    RefSeqi NP_011447.1. NM_001180933.1.

    3D structure databases

    ProteinModelPortali P53163.
    SMRi P53163. Positions 139-194.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33179. 27 interactions.
    DIPi DIP-6428N.
    IntActi P53163. 4 interactions.
    MINTi MINT-705819.
    STRINGi 4932.YGL068W.

    Proteomic databases

    MaxQBi P53163.
    PaxDbi P53163.
    PeptideAtlasi P53163.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL068W ; YGL068W ; YGL068W .
    GeneIDi 852811.
    KEGGi sce:YGL068W.

    Organism-specific databases

    CYGDi YGL068w.
    SGDi S000003036. MNP1.

    Phylogenomic databases

    eggNOGi COG0222.
    GeneTreei ENSGT00390000000190.
    HOGENOMi HOG000248814.
    KOi K02935.
    OMAi MYCTRYC.
    OrthoDBi EOG70GMV4.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30572-MONOMER.

    Miscellaneous databases

    NextBioi 972342.
    PROi P53163.

    Gene expression databases

    Genevestigatori P53163.

    Family and domain databases

    Gene3Di 3.30.1390.10. 1 hit.
    HAMAPi MF_00368. Ribosomal_L7_L12.
    InterProi IPR000206. Ribosomal_L7/12.
    IPR014719. Ribosomal_L7/12_C/ClpS-like.
    IPR013823. Ribosomal_L7/L12_C.
    IPR008932. Ribosomal_L7/L12_oligo.
    [Graphical view ]
    PANTHERi PTHR11809. PTHR11809. 1 hit.
    Pfami PF00542. Ribosomal_L12. 1 hit.
    [Graphical view ]
    ProDomi PD001326. Ribosomal_L7/L12_C. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF48300. SSF48300. 1 hit.
    SSF54736. SSF54736. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
      Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
      Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "A 22 kDa protein specific for yeast mitochondrial nucleoids is an unidentified putative ribosomal protein encoded in open reading frame YGL068W."
      Sato H., Miyakawa I.
      Protoplasma 223:175-182(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 34-48, SUBUNIT, SUBCELLULAR LOCATION.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: ATCC 76625 / YPH499.
    8. "Identification and comparative analysis of the large subunit mitochondrial ribosomal proteins of Neurospora crassa."
      Gan X., Arita K., Isono S., Kitakawa M., Yoshino K., Yonezawa K., Kato A., Inoue H., Isono K.
      FEMS Microbiol. Lett. 254:157-164(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT.
    9. "Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals new roles for protein glycosylation in eukaryotes."
      Kung L.A., Tao S.-C., Qian J., Smith M.G., Snyder M., Zhu H.
      Mol. Syst. Biol. 5:308-308(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiMNP1_YEAST
    AccessioniPrimary (citable) accession number: P53163
    Secondary accession number(s): D6VU73
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 6900 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Ribosomal proteins
      Ribosomal proteins families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3