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Protein

54S ribosomal protein L12, mitochondrial

Gene

MNP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.2 Publications

Miscellaneous

Present with 6900 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • mitochondrial translation Source: SGD

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-30572-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
54S ribosomal protein L12, mitochondrial
Alternative name(s):
Mitochondrial large ribosomal subunit protein bL12m1 Publication
Mitochondrial-nucleoid protein 1
Gene namesi
Name:MNP1
Synonyms:MRPL12
Ordered Locus Names:YGL068W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL068W
SGDiS000003036 MNP1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 33Mitochondrion1 PublicationAdd BLAST33
ChainiPRO_000003046034 – 19454S ribosomal protein L12, mitochondrialAdd BLAST161

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi34N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP53163
PaxDbiP53163
PRIDEiP53163

Interactioni

Subunit structurei

Component of the mitochondrial large ribosomal subunit (mt-LSU) (PubMed:15221522, PubMed:16451194). Mature yeast 74S mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S small subunit contains a 15S ribosomal RNA (15S mt-rRNA) and 34 different proteins. The 54S large subunit contains a 21S rRNA (21S mt-rRNA) and 46 different proteins (PubMed:24675956).1 Publication2 Publications

Protein-protein interaction databases

BioGridi33179, 38 interactors
DIPiDIP-6428N
IntActiP53163, 11 interactors
MINTiP53163
STRINGi4932.YGL068W

Structurei

3D structure databases

ProteinModelPortaliP53163
SMRiP53163
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000000190
HOGENOMiHOG000248814
InParanoidiP53163
KOiK02935
OMAiVDNAPKP
OrthoDBiEOG092C5NJO

Family and domain databases

CDDicd00387 Ribosomal_L7_L12, 1 hit
Gene3Di3.30.1390.10, 1 hit
HAMAPiMF_00368 Ribosomal_L7_L12, 1 hit
InterProiView protein in InterPro
IPR000206 Ribosomal_L7/12
IPR014719 Ribosomal_L7/12_C/ClpS-like
IPR013823 Ribosomal_L7/L12_C
IPR008932 Ribosomal_L7/L12_oligo
IPR036235 Ribosomal_L7/L12_oligo_N_sf
PfamiView protein in Pfam
PF00542 Ribosomal_L12, 1 hit
PF16320 Ribosomal_L12_N, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD001326 Ribosomal_L7/L12_C, 1 hit
SUPFAMiSSF48300 SSF48300, 1 hit
SSF54736 SSF54736, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53163-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLRILAKRS SSIWMKTRVT PALISPITIT TRFNSTTTTA PSHKDDVRPV
60 70 80 90 100
DPKISKIVQD ISQLTLLETS SLINELKTVL NIPEISMPMG GFMAGAAGAG
110 120 130 140 150
AGNVPSSTGE AGSGAEEEAK PEAKTVFTVK LDSFDTKTKA KVIKEVKGLL
160 170 180 190
GLSLVEAKKF VEAAPKVLKE NVAKDDAEKI KKTLEDLGAK VSLE
Length:194
Mass (Da):20,650
Last modified:October 1, 1996 - v1
Checksum:iD7892681B778B8A9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti34N → D AA sequence (PubMed:15221522).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72591 Genomic DNA Translation: CAA96773.1
BK006941 Genomic DNA Translation: DAA08034.1
PIRiS64075
RefSeqiNP_011447.1, NM_001180933.1

Genome annotation databases

EnsemblFungiiYGL068W; YGL068W; YGL068W
GeneIDi852811
KEGGisce:YGL068W

Similar proteinsi

Entry informationi

Entry nameiMNP1_YEAST
AccessioniPrimary (citable) accession number: P53163
Secondary accession number(s): D6VU73
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health