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Protein

54S ribosomal protein L12, mitochondrial

Gene

MNP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. structural constituent of ribosome Source: SGD

GO - Biological processi

  1. aerobic respiration Source: SGD
  2. translation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-30572-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
54S ribosomal protein L12, mitochondrial
Alternative name(s):
Mitochondrial-nucleoid protein 1
Gene namesi
Name:MNP1
Synonyms:MRPL12
Ordered Locus Names:YGL068W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGL068w.
SGDiS000003036. MNP1.

Subcellular locationi

Mitochondrion 4 Publications

GO - Cellular componenti

  1. mitochondrial nucleoid Source: SGD
  2. ribosome Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3333Mitochondrion1 PublicationAdd
BLAST
Chaini34 – 19416154S ribosomal protein L12, mitochondrialPRO_0000030460Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi34 – 341N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP53163.
PaxDbiP53163.
PeptideAtlasiP53163.

Expressioni

Gene expression databases

GenevestigatoriP53163.

Interactioni

Subunit structurei

Component of the mitochondrial large ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S).2 Publications

Protein-protein interaction databases

BioGridi33179. 29 interactions.
DIPiDIP-6428N.
IntActiP53163. 4 interactions.
MINTiMINT-705819.
STRINGi4932.YGL068W.

Structurei

3D structure databases

ProteinModelPortaliP53163.
SMRiP53163. Positions 139-194.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L7/L12P family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0222.
GeneTreeiENSGT00390000000190.
HOGENOMiHOG000248814.
InParanoidiP53163.
KOiK02935.
OMAiSSIIMKR.
OrthoDBiEOG70GMV4.

Family and domain databases

Gene3Di3.30.1390.10. 1 hit.
HAMAPiMF_00368. Ribosomal_L7_L12.
InterProiIPR000206. Ribosomal_L7/12.
IPR014719. Ribosomal_L7/12_C/ClpS-like.
IPR013823. Ribosomal_L7/L12_C.
IPR008932. Ribosomal_L7/L12_oligo.
[Graphical view]
PANTHERiPTHR11809. PTHR11809. 1 hit.
PfamiPF00542. Ribosomal_L12. 1 hit.
[Graphical view]
ProDomiPD001326. Ribosomal_L7/L12_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF48300. SSF48300. 1 hit.
SSF54736. SSF54736. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53163-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLRILAKRS SSIWMKTRVT PALISPITIT TRFNSTTTTA PSHKDDVRPV
60 70 80 90 100
DPKISKIVQD ISQLTLLETS SLINELKTVL NIPEISMPMG GFMAGAAGAG
110 120 130 140 150
AGNVPSSTGE AGSGAEEEAK PEAKTVFTVK LDSFDTKTKA KVIKEVKGLL
160 170 180 190
GLSLVEAKKF VEAAPKVLKE NVAKDDAEKI KKTLEDLGAK VSLE
Length:194
Mass (Da):20,650
Last modified:October 1, 1996 - v1
Checksum:iD7892681B778B8A9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti34 – 341N → D AA sequence (PubMed:15221522).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72591 Genomic DNA. Translation: CAA96773.1.
BK006941 Genomic DNA. Translation: DAA08034.1.
PIRiS64075.
RefSeqiNP_011447.1. NM_001180933.1.

Genome annotation databases

EnsemblFungiiYGL068W; YGL068W; YGL068W.
GeneIDi852811.
KEGGisce:YGL068W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72591 Genomic DNA. Translation: CAA96773.1.
BK006941 Genomic DNA. Translation: DAA08034.1.
PIRiS64075.
RefSeqiNP_011447.1. NM_001180933.1.

3D structure databases

ProteinModelPortaliP53163.
SMRiP53163. Positions 139-194.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33179. 29 interactions.
DIPiDIP-6428N.
IntActiP53163. 4 interactions.
MINTiMINT-705819.
STRINGi4932.YGL068W.

Proteomic databases

MaxQBiP53163.
PaxDbiP53163.
PeptideAtlasiP53163.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL068W; YGL068W; YGL068W.
GeneIDi852811.
KEGGisce:YGL068W.

Organism-specific databases

CYGDiYGL068w.
SGDiS000003036. MNP1.

Phylogenomic databases

eggNOGiCOG0222.
GeneTreeiENSGT00390000000190.
HOGENOMiHOG000248814.
InParanoidiP53163.
KOiK02935.
OMAiSSIIMKR.
OrthoDBiEOG70GMV4.

Enzyme and pathway databases

BioCyciYEAST:G3O-30572-MONOMER.

Miscellaneous databases

NextBioi972342.
PROiP53163.

Gene expression databases

GenevestigatoriP53163.

Family and domain databases

Gene3Di3.30.1390.10. 1 hit.
HAMAPiMF_00368. Ribosomal_L7_L12.
InterProiIPR000206. Ribosomal_L7/12.
IPR014719. Ribosomal_L7/12_C/ClpS-like.
IPR013823. Ribosomal_L7/L12_C.
IPR008932. Ribosomal_L7/L12_oligo.
[Graphical view]
PANTHERiPTHR11809. PTHR11809. 1 hit.
PfamiPF00542. Ribosomal_L12. 1 hit.
[Graphical view]
ProDomiPD001326. Ribosomal_L7/L12_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF48300. SSF48300. 1 hit.
SSF54736. SSF54736. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
    Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
    Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "A 22 kDa protein specific for yeast mitochondrial nucleoids is an unidentified putative ribosomal protein encoded in open reading frame YGL068W."
    Sato H., Miyakawa I.
    Protoplasma 223:175-182(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 34-48, SUBUNIT, SUBCELLULAR LOCATION.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  8. "Identification and comparative analysis of the large subunit mitochondrial ribosomal proteins of Neurospora crassa."
    Gan X., Arita K., Isono S., Kitakawa M., Yoshino K., Yonezawa K., Kato A., Inoue H., Isono K.
    FEMS Microbiol. Lett. 254:157-164(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  9. "Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals new roles for protein glycosylation in eukaryotes."
    Kung L.A., Tao S.-C., Qian J., Smith M.G., Snyder M., Zhu H.
    Mol. Syst. Biol. 5:308-308(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION.

Entry informationi

Entry nameiMNP1_YEAST
AccessioniPrimary (citable) accession number: P53163
Secondary accession number(s): D6VU73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: February 4, 2015
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6900 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Ribosomal proteins
    Ribosomal proteins families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.