ID   YGI2_YEAST              Reviewed;         381 AA.
AC   P53155; D6VU62; Q6Q536;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   27-MAR-2024, entry version 149.
DE   RecName: Full=Uncharacterized protein YGL082W;
GN   OrderedLocusNames=YGL082W;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9290212;
RX   DOI=10.1002/(sici)1097-0061(19970915)13:11<1077::aid-yea152>3.0.co;2-y;
RA   Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.;
RT   "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae
RT   chromosome VII.";
RL   Yeast 13:1077-1090(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169869;
RA   Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA   Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA   Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA   Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA   Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA   Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA   Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA   Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA   Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA   Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA   Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA   Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA   Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA   Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA   Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA   Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA   Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA   Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA   Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA   Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA   Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA   Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL   Nature 387:81-84(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [5]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [6]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [7]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth phosphoproteome
RT   analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}. Nucleus
CC       {ECO:0000269|PubMed:14562095}.
CC   -!- MISCELLANEOUS: Present with 7620 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
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DR   EMBL; Z72604; CAA96787.1; -; Genomic_DNA.
DR   EMBL; AY558581; AAS56907.1; -; Genomic_DNA.
DR   EMBL; BK006941; DAA08023.1; -; Genomic_DNA.
DR   PIR; S64089; S64089.
DR   RefSeq; NP_011433.3; NM_001180947.3.
DR   PDB; 6K6L; X-ray; 1.77 A; A/B=1-279.
DR   PDBsum; 6K6L; -.
DR   AlphaFoldDB; P53155; -.
DR   SMR; P53155; -.
DR   BioGRID; 33168; 32.
DR   IntAct; P53155; 4.
DR   MINT; P53155; -.
DR   STRING; 4932.YGL082W; -.
DR   iPTMnet; P53155; -.
DR   MaxQB; P53155; -.
DR   PaxDb; 4932-YGL082W; -.
DR   PeptideAtlas; P53155; -.
DR   TopDownProteomics; P53155; -.
DR   EnsemblFungi; YGL082W_mRNA; YGL082W; YGL082W.
DR   GeneID; 852798; -.
DR   KEGG; sce:YGL082W; -.
DR   AGR; SGD:S000003050; -.
DR   SGD; S000003050; YGL082W.
DR   VEuPathDB; FungiDB:YGL082W; -.
DR   eggNOG; KOG2427; Eukaryota.
DR   GeneTree; ENSGT00390000016607; -.
DR   HOGENOM; CLU_022566_0_0_1; -.
DR   InParanoid; P53155; -.
DR   OMA; GVEMSIF; -.
DR   OrthoDB; 9158at2759; -.
DR   BioCyc; YEAST:G3O-30583-MONOMER; -.
DR   BioGRID-ORCS; 852798; 0 hits in 10 CRISPR screens.
DR   PRO; PR:P53155; -.
DR   Proteomes; UP000002311; Chromosome VII.
DR   RNAct; P53155; Protein.
DR   GO; GO:0071944; C:cell periphery; HDA:SGD.
DR   GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR   GO; GO:0005634; C:nucleus; HDA:SGD.
DR   GO; GO:0005886; C:plasma membrane; HDA:SGD.
DR   GO; GO:0016807; F:cysteine-type carboxypeptidase activity; IBA:GO_Central.
DR   GO; GO:0004843; F:cysteine-type deubiquitinase activity; IEA:InterPro.
DR   GO; GO:1990380; F:K48-linked deubiquitinase activity; IBA:GO_Central.
DR   InterPro; IPR007518; MINDY.
DR   InterPro; IPR033979; MINDY_domain.
DR   PANTHER; PTHR18063; NF-E2 INDUCIBLE PROTEIN; 1.
DR   PANTHER; PTHR18063:SF6; UBIQUITIN CARBOXYL-TERMINAL HYDROLASE; 1.
DR   Pfam; PF04424; MINDY_DUB; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; Nucleus; Reference proteome.
FT   CHAIN           1..381
FT                   /note="Uncharacterized protein YGL082W"
FT                   /id="PRO_0000202757"
FT   REGION          331..381
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        341..381
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        263
FT                   /note="I -> T (in Ref. 4; AAS56907)"
FT                   /evidence="ECO:0000305"
FT   STRAND          7..12
FT                   /evidence="ECO:0007829|PDB:6K6L"
FT   STRAND          15..20
FT                   /evidence="ECO:0007829|PDB:6K6L"
FT   HELIX           29..39
FT                   /evidence="ECO:0007829|PDB:6K6L"
FT   HELIX           41..43
FT                   /evidence="ECO:0007829|PDB:6K6L"
FT   TURN            44..46
FT                   /evidence="ECO:0007829|PDB:6K6L"
FT   HELIX           48..55
FT                   /evidence="ECO:0007829|PDB:6K6L"
FT   HELIX           63..74
FT                   /evidence="ECO:0007829|PDB:6K6L"
FT   HELIX           87..90
FT                   /evidence="ECO:0007829|PDB:6K6L"
FT   HELIX           93..96
FT                   /evidence="ECO:0007829|PDB:6K6L"
FT   HELIX           112..119
FT                   /evidence="ECO:0007829|PDB:6K6L"
FT   STRAND          123..126
FT                   /evidence="ECO:0007829|PDB:6K6L"
FT   TURN            132..134
FT                   /evidence="ECO:0007829|PDB:6K6L"
FT   HELIX           136..142
FT                   /evidence="ECO:0007829|PDB:6K6L"
FT   HELIX           147..163
FT                   /evidence="ECO:0007829|PDB:6K6L"
FT   HELIX           170..186
FT                   /evidence="ECO:0007829|PDB:6K6L"
FT   HELIX           193..202
FT                   /evidence="ECO:0007829|PDB:6K6L"
FT   STRAND          208..213
FT                   /evidence="ECO:0007829|PDB:6K6L"
FT   STRAND          216..223
FT                   /evidence="ECO:0007829|PDB:6K6L"
FT   STRAND          226..230
FT                   /evidence="ECO:0007829|PDB:6K6L"
FT   HELIX           234..236
FT                   /evidence="ECO:0007829|PDB:6K6L"
FT   STRAND          244..246
FT                   /evidence="ECO:0007829|PDB:6K6L"
FT   STRAND          250..252
FT                   /evidence="ECO:0007829|PDB:6K6L"
SQ   SEQUENCE   381 AA;  43321 MW;  7F1047CF01FC40D5 CRC64;
     MDVTFLTKNV QINGTQFKIL LQNGQGECAL IALANVLLIS PAHARYAQEI SRLVRGKETV
     TLNELVQTLA DMGVQNPNGT DVDKQQLLQI LPQLYSGLNI NPEFNGSFED GVEMSIFRLY
     NVGIVHGWII DGDNDPNSYE HVSKYSYMGA QKVLVQSYEI QKNNAQFENS EQIQSDAPYL
     KSFLARSATQ LTEYGLTHLR EILVERSYAV LFRNDHFCTL YKNNGELFTL VTDPTYRNRK
     DINWQSLKSV NGSQDSYYTG NFIPTSLERT ETTATGQNES YISNPFSDQN TGHVTSNQVN
     SGASGVQQIE DDEELARRLQ EQEDMRAANN MQNGYANNGR NHQRERFERP EKNSKKNKFL
     PFNGSNKEKK RDKLKKNCVI M
//