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P53154 (GUP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycerol uptake protein 1
Gene names
Name:GUP1
Ordered Locus Names:YGL084C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length560 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in active uptake of glycerol driven by electrogenic proton symport. Ref.4

Subcellular location

Membrane; Multi-pass membrane protein Potential.

Miscellaneous

Present with 672 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the membrane-bound acyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 560560Glycerol uptake protein 1
PRO_0000213132

Regions

Topological domain1 – 4343Cytoplasmic Potential
Transmembrane44 – 6421Helical; Potential
Topological domain65 – 10137Extracellular Potential
Transmembrane102 – 12221Helical; Potential
Topological domain123 – 1319Cytoplasmic Potential
Transmembrane132 – 15221Helical; Potential
Topological domain153 – 276124Extracellular Potential
Transmembrane277 – 29721Helical; Potential
Topological domain298 – 32225Cytoplasmic Potential
Transmembrane323 – 34321Helical; Potential
Topological domain344 – 3529Extracellular Potential
Transmembrane353 – 37321Helical; Potential
Topological domain374 – 43259Cytoplasmic Potential
Transmembrane433 – 44917Helical; Potential
Topological domain450 – 4545Extracellular Potential
Transmembrane455 – 47420Helical; Potential
Topological domain475 – 48511Cytoplasmic Potential
Transmembrane486 – 50621Helical; Potential
Topological domain507 – 52620Extracellular Potential
Transmembrane527 – 54721Helical; Potential
Topological domain548 – 56013Cytoplasmic Potential

Sites

Active site4471 Potential

Sequences

Sequence LengthMass (Da)Tools
P53154 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: F8E9017D3E856A35

FASTA56065,340
        10         20         30         40         50         60 
MSLISILSPL ITSEGLDSRI KPSPKKDAST TTKPSLWKTT EFKFYYIAFL VVVPLMFYAG 

        70         80         90        100        110        120 
LQASSPENPN YARYERLLSQ GWLFGRKVDN SDSQYRFFRD NFALLSVLML VHTSIKRIVL 

       130        140        150        160        170        180 
YSTNITKLRF DLIFGLIFLV AAHGVNSIRI LAHMLILYAI AHVLKNFRRI ATISIWIYGI 

       190        200        210        220        230        240 
STLFINDNFR AYPFGNICSF LSPLDHWYRG IIPRWDVFFN FTLLRVLSYN LDFLERWENL 

       250        260        270        280        290        300 
QKKKSPSYES KEAKSAILLN ERARLTAAHP IQDYSLMNYI AYVTYTPLFI AGPIITFNDY 

       310        320        330        340        350        360 
VYQSKHTLPS INFKFIFYYA VRFVIALLSM EFILHFLHVV AISKTKAWEN DTPFQISMIG 

       370        380        390        400        410        420 
LFNLNIIWLK LLIPWRLFRL WALLDGIDTP ENMIRCVDNN YSSLAFWRAW HRSYNKWVVR 

       430        440        450        460        470        480 
YIYIPLGGSK NRVLTSLAVF SFVAIWHDIE LKLLLWGWLI VLFLLPEIFA TQIFSHYTDA 

       490        500        510        520        530        540 
VWYRHVCAVG AVFNIWVMMI ANLFGFCLGS DGTKKLLSDM FCTVSGFKFV ILASVSLFIA 

       550        560 
VQIMFEIREE EKRHGIYLKC 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"GUP1 and its close homologue GUP2, encoding multimembrane-spanning proteins involved in active glycerol uptake in Saccharomyces cerevisiae."
Holst B., Lunde C., Lages F., Oliveira R., Lucas C., Kielland-Brandt M.C.
Mol. Microbiol. 37:108-124(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
[7]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z72606 Genomic DNA. Translation: CAA96789.1.
BK006941 Genomic DNA. Translation: DAA08021.1.
PIRS64091.
RefSeqNP_011431.1. NM_001180949.1.

3D structure databases

ProteinModelPortalP53154.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33166. 282 interactions.
DIPDIP-4367N.
IntActP53154. 1 interaction.
MINTMINT-561950.
STRING4932.YGL084C.

Protein family/group databases

TCDB2.A.50.1.1. the glycerol uptake (gup) family.

Proteomic databases

MaxQBP53154.
PaxDbP53154.
PeptideAtlasP53154.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGL084C; YGL084C; YGL084C.
GeneID852796.
KEGGsce:YGL084C.

Organism-specific databases

CYGDYGL084c.
SGDS000003052. GUP1.

Phylogenomic databases

eggNOGCOG1696.
GeneTreeENSGT00530000063629.
HOGENOMHOG000182736.
OMAFEYREEE.
OrthoDBEOG7B5X4V.

Enzyme and pathway databases

BioCycYEAST:G3O-30585-MONOMER.

Gene expression databases

GenevestigatorP53154.

Family and domain databases

InterProIPR004299. MBOAT_fam.
[Graphical view]
PfamPF03062. MBOAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio972303.
PROP53154.

Entry information

Entry nameGUP1_YEAST
AccessionPrimary (citable) accession number: P53154
Secondary accession number(s): D6VU60
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 14, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families