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Protein

Glycerol uptake protein 1

Gene

GUP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in active uptake of glycerol driven by electrogenic proton symport.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei447 – 4471Sequence Analysis

GO - Molecular functioni

  1. O-acyltransferase activity Source: SGD
  2. symporter activity Source: UniProtKB-KW

GO - Biological processi

  1. glycerol catabolic process Source: SGD
  2. glycerol transport Source: SGD
  3. GPI anchor biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Biological processi

Symport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30585-MONOMER.

Protein family/group databases

TCDBi2.A.50.1.1. the glycerol uptake (gup) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol uptake protein 1
Gene namesi
Name:GUP1
Ordered Locus Names:YGL084C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGL084c.
SGDiS000003052. GUP1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4343CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei44 – 6421HelicalSequence AnalysisAdd
BLAST
Topological domaini65 – 10137ExtracellularSequence AnalysisAdd
BLAST
Transmembranei102 – 12221HelicalSequence AnalysisAdd
BLAST
Topological domaini123 – 1319CytoplasmicSequence Analysis
Transmembranei132 – 15221HelicalSequence AnalysisAdd
BLAST
Topological domaini153 – 276124ExtracellularSequence AnalysisAdd
BLAST
Transmembranei277 – 29721HelicalSequence AnalysisAdd
BLAST
Topological domaini298 – 32225CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei323 – 34321HelicalSequence AnalysisAdd
BLAST
Topological domaini344 – 3529ExtracellularSequence Analysis
Transmembranei353 – 37321HelicalSequence AnalysisAdd
BLAST
Topological domaini374 – 43259CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei433 – 44917HelicalSequence AnalysisAdd
BLAST
Topological domaini450 – 4545ExtracellularSequence Analysis
Transmembranei455 – 47420HelicalSequence AnalysisAdd
BLAST
Topological domaini475 – 48511CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei486 – 50621HelicalSequence AnalysisAdd
BLAST
Topological domaini507 – 52620ExtracellularSequence AnalysisAdd
BLAST
Transmembranei527 – 54721HelicalSequence AnalysisAdd
BLAST
Topological domaini548 – 56013CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: SGD
  2. integral component of membrane Source: UniProtKB-KW
  3. membrane Source: SGD
  4. plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 560560Glycerol uptake protein 1PRO_0000213132Add
BLAST

Proteomic databases

MaxQBiP53154.
PaxDbiP53154.
PeptideAtlasiP53154.

Expressioni

Gene expression databases

GenevestigatoriP53154.

Interactioni

Protein-protein interaction databases

BioGridi33166. 282 interactions.
DIPiDIP-4367N.
IntActiP53154. 1 interaction.
MINTiMINT-561950.
STRINGi4932.YGL084C.

Structurei

3D structure databases

ProteinModelPortaliP53154.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1696.
GeneTreeiENSGT00530000063629.
HOGENOMiHOG000182736.
InParanoidiP53154.
OMAiSFNITML.
OrthoDBiEOG7B5X4V.

Family and domain databases

InterProiIPR004299. MBOAT_fam.
[Graphical view]
PfamiPF03062. MBOAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53154-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLISILSPL ITSEGLDSRI KPSPKKDAST TTKPSLWKTT EFKFYYIAFL
60 70 80 90 100
VVVPLMFYAG LQASSPENPN YARYERLLSQ GWLFGRKVDN SDSQYRFFRD
110 120 130 140 150
NFALLSVLML VHTSIKRIVL YSTNITKLRF DLIFGLIFLV AAHGVNSIRI
160 170 180 190 200
LAHMLILYAI AHVLKNFRRI ATISIWIYGI STLFINDNFR AYPFGNICSF
210 220 230 240 250
LSPLDHWYRG IIPRWDVFFN FTLLRVLSYN LDFLERWENL QKKKSPSYES
260 270 280 290 300
KEAKSAILLN ERARLTAAHP IQDYSLMNYI AYVTYTPLFI AGPIITFNDY
310 320 330 340 350
VYQSKHTLPS INFKFIFYYA VRFVIALLSM EFILHFLHVV AISKTKAWEN
360 370 380 390 400
DTPFQISMIG LFNLNIIWLK LLIPWRLFRL WALLDGIDTP ENMIRCVDNN
410 420 430 440 450
YSSLAFWRAW HRSYNKWVVR YIYIPLGGSK NRVLTSLAVF SFVAIWHDIE
460 470 480 490 500
LKLLLWGWLI VLFLLPEIFA TQIFSHYTDA VWYRHVCAVG AVFNIWVMMI
510 520 530 540 550
ANLFGFCLGS DGTKKLLSDM FCTVSGFKFV ILASVSLFIA VQIMFEIREE
560
EKRHGIYLKC
Length:560
Mass (Da):65,340
Last modified:October 1, 1996 - v1
Checksum:iF8E9017D3E856A35
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72606 Genomic DNA. Translation: CAA96789.1.
BK006941 Genomic DNA. Translation: DAA08021.1.
PIRiS64091.
RefSeqiNP_011431.1. NM_001180949.1.

Genome annotation databases

EnsemblFungiiYGL084C; YGL084C; YGL084C.
GeneIDi852796.
KEGGisce:YGL084C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72606 Genomic DNA. Translation: CAA96789.1.
BK006941 Genomic DNA. Translation: DAA08021.1.
PIRiS64091.
RefSeqiNP_011431.1. NM_001180949.1.

3D structure databases

ProteinModelPortaliP53154.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33166. 282 interactions.
DIPiDIP-4367N.
IntActiP53154. 1 interaction.
MINTiMINT-561950.
STRINGi4932.YGL084C.

Protein family/group databases

TCDBi2.A.50.1.1. the glycerol uptake (gup) family.

Proteomic databases

MaxQBiP53154.
PaxDbiP53154.
PeptideAtlasiP53154.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL084C; YGL084C; YGL084C.
GeneIDi852796.
KEGGisce:YGL084C.

Organism-specific databases

CYGDiYGL084c.
SGDiS000003052. GUP1.

Phylogenomic databases

eggNOGiCOG1696.
GeneTreeiENSGT00530000063629.
HOGENOMiHOG000182736.
InParanoidiP53154.
OMAiSFNITML.
OrthoDBiEOG7B5X4V.

Enzyme and pathway databases

BioCyciYEAST:G3O-30585-MONOMER.

Miscellaneous databases

NextBioi972303.
PROiP53154.

Gene expression databases

GenevestigatoriP53154.

Family and domain databases

InterProiIPR004299. MBOAT_fam.
[Graphical view]
PfamiPF03062. MBOAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
    Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
    Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "GUP1 and its close homologue GUP2, encoding multimembrane-spanning proteins involved in active glycerol uptake in Saccharomyces cerevisiae."
    Holst B., Lunde C., Lages F., Oliveira R., Lucas C., Kielland-Brandt M.C.
    Mol. Microbiol. 37:108-124(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGUP1_YEAST
AccessioniPrimary (citable) accession number: P53154
Secondary accession number(s): D6VU60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 7, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 672 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.