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P53153 (LCL3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable endonuclease LCL3

EC=3.1.-.-
Alternative name(s):
Long chronological lifespan protein 3
Gene names
Name:LCL3
Ordered Locus Names:YGL085W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length274 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Subcellular location

Mitochondrion. Membrane; Single-pass membrane protein Potential Ref.4.

Induction

By DNA-damaging agent methyl methanesulphonate (MMS) (at protein level). Ref.7

Disruption phenotype

Leads to long chronological lifespan. Ref.6

Miscellaneous

Present with 704 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the LCL3 family.

Contains 1 TNase-like domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

SHO1P400732EBI-23857,EBI-18140

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 274274Probable endonuclease LCL3
PRO_0000215285

Regions

Transmembrane15 – 3218Helical; Potential
Domain53 – 261209TNase-like

Sites

Active site1511 By similarity
Active site1591 By similarity
Active site1991 By similarity
Metal binding1561Calcium By similarity

Sequences

Sequence LengthMass (Da)Tools
P53153 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 38C1B01FEF4838A0

FASTA27432,112
        10         20         30         40         50         60 
MREGDSNSKK SADVAVLSII LTGSTLTLIY TYKRYLTQFK RTNDIPRRIF RKHWLYGKVT 

        70         80         90        100        110        120 
SVGDGDNFHF FHMPGGIRGG WGWLRPVPQM IKNDSTAEKL VGDSRNMRFF NFNWITHGRS 

       130        140        150        160        170        180 
TKSKIQKAKS QFLKLNVPYK NRKNLPTIPI RLCGIDAPER AHFGNPAQPF GNEALIWLQN 

       190        200        210        220        230        240 
RILGKKVWVK PLSIDQYNRC VARVSYWDWF GGWKDLSLEM LKDGLAVVYE GKVNTEFDDR 

       250        260        270 
EDKYRYYEFL ARSRKKGLWI QNKFETPGEY KKRI 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"A microarray-based genetic screen for yeast chronological aging factors."
Matecic M., Smith D.L., Pan X., Maqani N., Bekiranov S., Boeke J.D., Smith J.S.
PLoS Genet. 6:E1000921-E1000921(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
[7]"Global protein expression profiling of budding yeast in response to DNA damage."
Lee M.-W., Kim B.-J., Choi H.-K., Ryu M.-J., Kim S.-B., Kang K.-M., Cho E.-J., Youn H.-D., Huh W.-K., Kim S.-T.
Yeast 24:145-154(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z72607 Genomic DNA. Translation: CAA96790.1.
BK006941 Genomic DNA. Translation: DAA08020.1.
PIRS64092.
RefSeqNP_011430.1. NM_001180950.1.

3D structure databases

ProteinModelPortalP53153.
SMRP53153. Positions 147-260.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33165. 7 interactions.
IntActP53153. 3 interactions.
MINTMINT-1549028.
STRING4932.YGL085W.

Proteomic databases

PaxDbP53153.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGL085W; YGL085W; YGL085W.
GeneID852795.
KEGGsce:YGL085W.

Organism-specific databases

CYGDYGL085w.
SGDS000003053. LCL3.

Phylogenomic databases

eggNOGCOG1525.
OMANFHLFHT.
OrthoDBEOG76MKMM.

Enzyme and pathway databases

BioCycYEAST:G3O-30586-MONOMER.

Gene expression databases

GenevestigatorP53153.

Family and domain databases

Gene3D2.40.50.90. 2 hits.
InterProIPR016071. Staphylococal_nuclease_OB-fold.
[Graphical view]
PfamPF00565. SNase. 1 hit.
[Graphical view]
SMARTSM00318. SNc. 1 hit.
[Graphical view]
SUPFAMSSF50199. SSF50199. 2 hits.
PROSITEPS50830. TNASE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio972300.

Entry information

Entry nameLCL3_YEAST
AccessionPrimary (citable) accession number: P53153
Secondary accession number(s): D6VU59
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families