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P53153

- LCL3_YEAST

UniProt

P53153 - LCL3_YEAST

Protein

Probable endonuclease LCL3

Gene

LCL3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei151 – 1511PROSITE-ProRule annotation
    Metal bindingi156 – 1561CalciumPROSITE-ProRule annotation
    Active sitei159 – 1591PROSITE-ProRule annotation
    Active sitei199 – 1991PROSITE-ProRule annotation

    GO - Molecular functioni

    1. endonuclease activity Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. nucleic acid binding Source: InterPro
    4. protein binding Source: IntAct

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30586-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable endonuclease LCL3 (EC:3.1.-.-)
    Alternative name(s):
    Long chronological lifespan protein 3
    Gene namesi
    Name:LCL3
    Ordered Locus Names:YGL085W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGL085w.
    SGDiS000003053. LCL3.

    Subcellular locationi

    Mitochondrion 1 Publication. Membrane Curated; Single-pass membrane protein Curated

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane, Mitochondrion

    Pathology & Biotechi

    Disruption phenotypei

    Leads to long chronological lifespan.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 274274Probable endonuclease LCL3PRO_0000215285Add
    BLAST

    Proteomic databases

    MaxQBiP53153.
    PaxDbiP53153.

    Expressioni

    Inductioni

    By DNA-damaging agent methyl methanesulphonate (MMS) (at protein level).1 Publication

    Gene expression databases

    GenevestigatoriP53153.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SHO1P400732EBI-23857,EBI-18140

    Protein-protein interaction databases

    BioGridi33165. 7 interactions.
    IntActiP53153. 3 interactions.
    MINTiMINT-1549028.
    STRINGi4932.YGL085W.

    Structurei

    3D structure databases

    ProteinModelPortaliP53153.
    SMRiP53153. Positions 147-260.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei15 – 3218HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini53 – 261209TNase-likePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the LCL3 family.Curated
    Contains 1 TNase-like domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1525.
    OMAiNFHLFHT.
    OrthoDBiEOG76MKMM.

    Family and domain databases

    Gene3Di2.40.50.90. 2 hits.
    InterProiIPR016071. Staphylococal_nuclease_OB-fold.
    [Graphical view]
    PfamiPF00565. SNase. 1 hit.
    [Graphical view]
    SMARTiSM00318. SNc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50199. SSF50199. 2 hits.
    PROSITEiPS50830. TNASE_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53153-1 [UniParc]FASTAAdd to Basket

    « Hide

    MREGDSNSKK SADVAVLSII LTGSTLTLIY TYKRYLTQFK RTNDIPRRIF    50
    RKHWLYGKVT SVGDGDNFHF FHMPGGIRGG WGWLRPVPQM IKNDSTAEKL 100
    VGDSRNMRFF NFNWITHGRS TKSKIQKAKS QFLKLNVPYK NRKNLPTIPI 150
    RLCGIDAPER AHFGNPAQPF GNEALIWLQN RILGKKVWVK PLSIDQYNRC 200
    VARVSYWDWF GGWKDLSLEM LKDGLAVVYE GKVNTEFDDR EDKYRYYEFL 250
    ARSRKKGLWI QNKFETPGEY KKRI 274
    Length:274
    Mass (Da):32,112
    Last modified:October 1, 1996 - v1
    Checksum:i38C1B01FEF4838A0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72607 Genomic DNA. Translation: CAA96790.1.
    BK006941 Genomic DNA. Translation: DAA08020.1.
    PIRiS64092.
    RefSeqiNP_011430.1. NM_001180950.1.

    Genome annotation databases

    EnsemblFungiiYGL085W; YGL085W; YGL085W.
    GeneIDi852795.
    KEGGisce:YGL085W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72607 Genomic DNA. Translation: CAA96790.1 .
    BK006941 Genomic DNA. Translation: DAA08020.1 .
    PIRi S64092.
    RefSeqi NP_011430.1. NM_001180950.1.

    3D structure databases

    ProteinModelPortali P53153.
    SMRi P53153. Positions 147-260.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33165. 7 interactions.
    IntActi P53153. 3 interactions.
    MINTi MINT-1549028.
    STRINGi 4932.YGL085W.

    Proteomic databases

    MaxQBi P53153.
    PaxDbi P53153.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL085W ; YGL085W ; YGL085W .
    GeneIDi 852795.
    KEGGi sce:YGL085W.

    Organism-specific databases

    CYGDi YGL085w.
    SGDi S000003053. LCL3.

    Phylogenomic databases

    eggNOGi COG1525.
    OMAi NFHLFHT.
    OrthoDBi EOG76MKMM.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30586-MONOMER.

    Miscellaneous databases

    NextBioi 972300.

    Gene expression databases

    Genevestigatori P53153.

    Family and domain databases

    Gene3Di 2.40.50.90. 2 hits.
    InterProi IPR016071. Staphylococal_nuclease_OB-fold.
    [Graphical view ]
    Pfami PF00565. SNase. 1 hit.
    [Graphical view ]
    SMARTi SM00318. SNc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50199. SSF50199. 2 hits.
    PROSITEi PS50830. TNASE_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
      Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
      Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "A microarray-based genetic screen for yeast chronological aging factors."
      Matecic M., Smith D.L., Pan X., Maqani N., Bekiranov S., Boeke J.D., Smith J.S.
      PLoS Genet. 6:E1000921-E1000921(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    7. "Global protein expression profiling of budding yeast in response to DNA damage."
      Lee M.-W., Kim B.-J., Choi H.-K., Ryu M.-J., Kim S.-B., Kang K.-M., Cho E.-J., Youn H.-D., Huh W.-K., Kim S.-T.
      Yeast 24:145-154(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.

    Entry informationi

    Entry nameiLCL3_YEAST
    AccessioniPrimary (citable) accession number: P53153
    Secondary accession number(s): D6VU59
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 704 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3