P53153 (LCL3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable endonuclease LCL3
EC=3.1.-.-

Alternative name(s):
Long chronological lifespan protein 3

Gene names

Name:	LCL3
Ordered Locus Names:	YGL085W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Mitochondrion. Membrane; Single-pass membrane protein Potential Ref.4.
Induction	By DNA-damaging agent methyl methanesulphonate (MMS) (at protein level). Ref.7
Disruption phenotype	Leads to long chronological lifespan. Ref.6
Miscellaneous	Present with 704 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the LCL3 family. Contains 1 TNase-like domain.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion
Domain	Transmembrane Transmembrane helix
Ligand	Calcium Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrion Inferred from direct assay Ref.4. Source: SGD
Molecular_function	endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RVS167	P39743	1	EBI-23857,EBI-14500
SSU81	P40073	1	EBI-23857,EBI-18140

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 274	274	Probable endonuclease LCL3		PRO_0000215285
Regions
	Transmembrane	15 – 32	18	Helical; Potential
	Domain	53 – 261	209	TNase-like
Sites
	Active site	151	1	By similarity
	Active site	159	1	By similarity
	Active site	199	1	By similarity
	Metal binding	156	1	Calcium By similarity

Sequences

	Sequence		Length	Mass (Da)
	P53153 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 38C1B01FEF4838A0	FASTA	274	32,112
	10 20 30 40 50 60 MREGDSNSKK SADVAVLSII LTGSTLTLIY TYKRYLTQFK RTNDIPRRIF RKHWLYGKVT 70 80 90 100 110 120 SVGDGDNFHF FHMPGGIRGG WGWLRPVPQM IKNDSTAEKL VGDSRNMRFF NFNWITHGRS 130 140 150 160 170 180 TKSKIQKAKS QFLKLNVPYK NRKNLPTIPI RLCGIDAPER AHFGNPAQPF GNEALIWLQN 190 200 210 220 230 240 RILGKKVWVK PLSIDQYNRC VARVSYWDWF GGWKDLSLEM LKDGLAVVYE GKVNTEFDDR 250 260 270 EDKYRYYEFL ARSRKKGLWI QNKFETPGEY KKRI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII." Rieger M., Brueckner M., Schaefer M., Mueller-Auer S. Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A microarray-based genetic screen for yeast chronological aging factors." Matecic M., Smith D.L., Pan X., Maqani N., Bekiranov S., Boeke J.D., Smith J.S. PLoS Genet. 6:E1000921-E1000921(2010) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE.
[7]	"Global protein expression profiling of budding yeast in response to DNA damage." Lee M.-W., Kim B.-J., Choi H.-K., Ryu M.-J., Kim S.-B., Kang K.-M., Cho E.-J., Youn H.-D., Huh W.-K., Kim S.-T. Yeast 24:145-154(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z72607 Genomic DNA. Translation: CAA96790.1. BK006941 Genomic DNA. Translation: DAA08020.1.
PIR	S64092.
RefSeq	NP_011430.1. NM_001180950.1.
3D structure databases
ProteinModelPortal	P53153.
SMR	P53153. Positions 147-260.
ModBase	Search...
Protein-protein interaction databases
IntAct	P53153. 2 interactions.
MINT	MINT-1549028.
STRING	4932.YGL085W.
Proteomic databases
PaxDb	P53153.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL085W; YGL085W; YGL085W.
GeneID	852795.
KEGG	sce:YGL085W.
Organism-specific databases
CYGD	YGL085w.
SGD	S000003053. LCL3.
Phylogenomic databases
eggNOG	COG1525.
OMA	CGVDAPE.
OrthoDB	EOG4QG0PC.
Gene expression databases
Genevestigator	P53153.
GermOnline	YGL085W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	2.40.50.90. 2 hits.
InterPro	IPR016071. Staphylococal_nuclease_OB-fold. IPR006021. Staphylococcal_nuclease. [Graphical view]
Pfam	PF00565. SNase. 1 hit. [Graphical view]
SMART	SM00318. SNc. 1 hit. [Graphical view]
SUPFAM	SSF50199. Staphylococal_nuclease_OB-fold. 1 hit.
PROSITE	PS01123. TNASE_1. False negative. PS01284. TNASE_2. False negative. PS50830. TNASE_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	972300.

Entry information

Entry name

LCL3_YEAST

Accession

Primary (citable) accession number: P53153
Secondary accession number(s): D6VU59

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program