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P53153

- LCL3_YEAST

UniProt

P53153 - LCL3_YEAST

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Protein
Probable endonuclease LCL3
Gene
LCL3, YGL085W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei151 – 1511 By similarity
Metal bindingi156 – 1561Calcium By similarity
Active sitei159 – 1591 By similarity
Active sitei199 – 1991 By similarity

GO - Molecular functioni

  1. endonuclease activity Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. nucleic acid binding Source: InterPro
  4. protein binding Source: IntAct
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30586-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable endonuclease LCL3 (EC:3.1.-.-)
Alternative name(s):
Long chronological lifespan protein 3
Gene namesi
Name:LCL3
Ordered Locus Names:YGL085W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGL085w.
SGDiS000003053. LCL3.

Subcellular locationi

Mitochondrion. Membrane; Single-pass membrane protein Reviewed prediction 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei15 – 3218Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Leads to long chronological lifespan.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 274274Probable endonuclease LCL3
PRO_0000215285Add
BLAST

Proteomic databases

MaxQBiP53153.
PaxDbiP53153.

Expressioni

Inductioni

By DNA-damaging agent methyl methanesulphonate (MMS) (at protein level).1 Publication

Gene expression databases

GenevestigatoriP53153.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
SHO1P400732EBI-23857,EBI-18140

Protein-protein interaction databases

BioGridi33165. 7 interactions.
IntActiP53153. 3 interactions.
MINTiMINT-1549028.
STRINGi4932.YGL085W.

Structurei

3D structure databases

ProteinModelPortaliP53153.
SMRiP53153. Positions 147-260.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini53 – 261209TNase-like
Add
BLAST

Sequence similaritiesi

Belongs to the LCL3 family.
Contains 1 TNase-like domain.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1525.
OMAiNFHLFHT.
OrthoDBiEOG76MKMM.

Family and domain databases

Gene3Di2.40.50.90. 2 hits.
InterProiIPR016071. Staphylococal_nuclease_OB-fold.
[Graphical view]
PfamiPF00565. SNase. 1 hit.
[Graphical view]
SMARTiSM00318. SNc. 1 hit.
[Graphical view]
SUPFAMiSSF50199. SSF50199. 2 hits.
PROSITEiPS50830. TNASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53153-1 [UniParc]FASTAAdd to Basket

« Hide

MREGDSNSKK SADVAVLSII LTGSTLTLIY TYKRYLTQFK RTNDIPRRIF    50
RKHWLYGKVT SVGDGDNFHF FHMPGGIRGG WGWLRPVPQM IKNDSTAEKL 100
VGDSRNMRFF NFNWITHGRS TKSKIQKAKS QFLKLNVPYK NRKNLPTIPI 150
RLCGIDAPER AHFGNPAQPF GNEALIWLQN RILGKKVWVK PLSIDQYNRC 200
VARVSYWDWF GGWKDLSLEM LKDGLAVVYE GKVNTEFDDR EDKYRYYEFL 250
ARSRKKGLWI QNKFETPGEY KKRI 274
Length:274
Mass (Da):32,112
Last modified:October 1, 1996 - v1
Checksum:i38C1B01FEF4838A0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z72607 Genomic DNA. Translation: CAA96790.1.
BK006941 Genomic DNA. Translation: DAA08020.1.
PIRiS64092.
RefSeqiNP_011430.1. NM_001180950.1.

Genome annotation databases

EnsemblFungiiYGL085W; YGL085W; YGL085W.
GeneIDi852795.
KEGGisce:YGL085W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z72607 Genomic DNA. Translation: CAA96790.1 .
BK006941 Genomic DNA. Translation: DAA08020.1 .
PIRi S64092.
RefSeqi NP_011430.1. NM_001180950.1.

3D structure databases

ProteinModelPortali P53153.
SMRi P53153. Positions 147-260.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33165. 7 interactions.
IntActi P53153. 3 interactions.
MINTi MINT-1549028.
STRINGi 4932.YGL085W.

Proteomic databases

MaxQBi P53153.
PaxDbi P53153.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGL085W ; YGL085W ; YGL085W .
GeneIDi 852795.
KEGGi sce:YGL085W.

Organism-specific databases

CYGDi YGL085w.
SGDi S000003053. LCL3.

Phylogenomic databases

eggNOGi COG1525.
OMAi NFHLFHT.
OrthoDBi EOG76MKMM.

Enzyme and pathway databases

BioCyci YEAST:G3O-30586-MONOMER.

Miscellaneous databases

NextBioi 972300.

Gene expression databases

Genevestigatori P53153.

Family and domain databases

Gene3Di 2.40.50.90. 2 hits.
InterProi IPR016071. Staphylococal_nuclease_OB-fold.
[Graphical view ]
Pfami PF00565. SNase. 1 hit.
[Graphical view ]
SMARTi SM00318. SNc. 1 hit.
[Graphical view ]
SUPFAMi SSF50199. SSF50199. 2 hits.
PROSITEi PS50830. TNASE_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
    Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
    Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "A microarray-based genetic screen for yeast chronological aging factors."
    Matecic M., Smith D.L., Pan X., Maqani N., Bekiranov S., Boeke J.D., Smith J.S.
    PLoS Genet. 6:E1000921-E1000921(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  7. "Global protein expression profiling of budding yeast in response to DNA damage."
    Lee M.-W., Kim B.-J., Choi H.-K., Ryu M.-J., Kim S.-B., Kang K.-M., Cho E.-J., Youn H.-D., Huh W.-K., Kim S.-T.
    Yeast 24:145-154(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiLCL3_YEAST
AccessioniPrimary (citable) accession number: P53153
Secondary accession number(s): D6VU59
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 704 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

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