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Protein

Probable endonuclease LCL3

Gene

LCL3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei151 – 1511PROSITE-ProRule annotation
Metal bindingi156 – 1561CalciumPROSITE-ProRule annotation
Active sitei159 – 1591PROSITE-ProRule annotation
Active sitei199 – 1991PROSITE-ProRule annotation

GO - Molecular functioni

  1. endonuclease activity Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30586-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable endonuclease LCL3 (EC:3.1.-.-)
Alternative name(s):
Long chronological lifespan protein 3
Gene namesi
Name:LCL3
Ordered Locus Names:YGL085W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome VII

Organism-specific databases

CYGDiYGL085w.
SGDiS000003053. LCL3.

Subcellular locationi

Mitochondrion 1 Publication. Membrane Curated; Single-pass membrane protein Curated

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei15 – 3218HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Leads to long chronological lifespan.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 274274Probable endonuclease LCL3PRO_0000215285Add
BLAST

Proteomic databases

MaxQBiP53153.
PaxDbiP53153.

Expressioni

Inductioni

By DNA-damaging agent methyl methanesulphonate (MMS) (at protein level).1 Publication

Gene expression databases

GenevestigatoriP53153.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
SHO1P400732EBI-23857,EBI-18140

Protein-protein interaction databases

BioGridi33165. 8 interactions.
IntActiP53153. 3 interactions.
MINTiMINT-1549028.
STRINGi4932.YGL085W.

Structurei

3D structure databases

ProteinModelPortaliP53153.
SMRiP53153. Positions 147-260.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini53 – 261209TNase-likePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the LCL3 family.Curated
Contains 1 TNase-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1525.
InParanoidiP53153.
OMAiNFHLFHT.
OrthoDBiEOG76MKMM.

Family and domain databases

Gene3Di2.40.50.90. 2 hits.
InterProiIPR016071. Staphylococal_nuclease_OB-fold.
[Graphical view]
PfamiPF00565. SNase. 1 hit.
[Graphical view]
SMARTiSM00318. SNc. 1 hit.
[Graphical view]
SUPFAMiSSF50199. SSF50199. 2 hits.
PROSITEiPS50830. TNASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53153-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MREGDSNSKK SADVAVLSII LTGSTLTLIY TYKRYLTQFK RTNDIPRRIF
60 70 80 90 100
RKHWLYGKVT SVGDGDNFHF FHMPGGIRGG WGWLRPVPQM IKNDSTAEKL
110 120 130 140 150
VGDSRNMRFF NFNWITHGRS TKSKIQKAKS QFLKLNVPYK NRKNLPTIPI
160 170 180 190 200
RLCGIDAPER AHFGNPAQPF GNEALIWLQN RILGKKVWVK PLSIDQYNRC
210 220 230 240 250
VARVSYWDWF GGWKDLSLEM LKDGLAVVYE GKVNTEFDDR EDKYRYYEFL
260 270
ARSRKKGLWI QNKFETPGEY KKRI
Length:274
Mass (Da):32,112
Last modified:October 1, 1996 - v1
Checksum:i38C1B01FEF4838A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72607 Genomic DNA. Translation: CAA96790.1.
BK006941 Genomic DNA. Translation: DAA08020.1.
PIRiS64092.
RefSeqiNP_011430.1. NM_001180950.1.

Genome annotation databases

EnsemblFungiiYGL085W; YGL085W; YGL085W.
GeneIDi852795.
KEGGisce:YGL085W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72607 Genomic DNA. Translation: CAA96790.1.
BK006941 Genomic DNA. Translation: DAA08020.1.
PIRiS64092.
RefSeqiNP_011430.1. NM_001180950.1.

3D structure databases

ProteinModelPortaliP53153.
SMRiP53153. Positions 147-260.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33165. 8 interactions.
IntActiP53153. 3 interactions.
MINTiMINT-1549028.
STRINGi4932.YGL085W.

Proteomic databases

MaxQBiP53153.
PaxDbiP53153.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL085W; YGL085W; YGL085W.
GeneIDi852795.
KEGGisce:YGL085W.

Organism-specific databases

CYGDiYGL085w.
SGDiS000003053. LCL3.

Phylogenomic databases

eggNOGiCOG1525.
InParanoidiP53153.
OMAiNFHLFHT.
OrthoDBiEOG76MKMM.

Enzyme and pathway databases

BioCyciYEAST:G3O-30586-MONOMER.

Miscellaneous databases

NextBioi972300.

Gene expression databases

GenevestigatoriP53153.

Family and domain databases

Gene3Di2.40.50.90. 2 hits.
InterProiIPR016071. Staphylococal_nuclease_OB-fold.
[Graphical view]
PfamiPF00565. SNase. 1 hit.
[Graphical view]
SMARTiSM00318. SNc. 1 hit.
[Graphical view]
SUPFAMiSSF50199. SSF50199. 2 hits.
PROSITEiPS50830. TNASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
    Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
    Yeast 13:1077-1090(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "A microarray-based genetic screen for yeast chronological aging factors."
    Matecic M., Smith D.L., Pan X., Maqani N., Bekiranov S., Boeke J.D., Smith J.S.
    PLoS Genet. 6:E1000921-E1000921(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  7. "Global protein expression profiling of budding yeast in response to DNA damage."
    Lee M.-W., Kim B.-J., Choi H.-K., Ryu M.-J., Kim S.-B., Kang K.-M., Cho E.-J., Youn H.-D., Huh W.-K., Kim S.-T.
    Yeast 24:145-154(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiLCL3_YEAST
AccessioniPrimary (citable) accession number: P53153
Secondary accession number(s): D6VU59
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 4, 2015
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 704 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.