P53152 (MMS2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme variant MMS2
Short name=UEV MMS2

Gene names

Name:	MMS2
Ordered Locus Names:	YGL087C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	137 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has a role in the DNA error-free postreplication repair (PRR) pathway. Lacks catalytic activity by itself. The UBC13/MMS2 heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'.
Subunit structure	Heterodimer with UBC13.
Miscellaneous	Present with 2760 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Molecular function	Ligase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	free ubiquitin chain polymerization Inferred from direct assay. Source: SGD postreplication repair Inferred from mutant phenotype Ref.6Ref.1. Source: SGD protein polyubiquitination Inferred from physical interaction. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD ubiquitin conjugating enzyme complex Inferred from physical interaction Ref.6. Source: SGD
Molecular function	acid-amino acid ligase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
UBC13	P52490	6	EBI-11035,EBI-19777

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 137

137

Ubiquitin-conjugating enzyme variant MMS2

PRO_0000082598

Amino acid modifications

Modified residue

71

1

Phosphoserine Ref.8

Experimental info

Mutagenesis

8

1

F → A: Strongly reduces UBC13 binding and interferes with error-free DNA repair. Ref.9

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

137

Helix Strand Turn

Details...

Helix

6 – 18

13

Beta strand

26 – 32

7

Beta strand

40 – 46

7

Beta strand

49 – 51

3

Turn

52 – 55

4

Beta strand

57 – 63

7

Turn

66 – 70

5

Beta strand

74 – 79

6

Turn

88 – 90

3

Helix

98 – 101

4

Helix

109 – 120

12

Helix

123 – 126

4

Sequences

	Sequence		Length	Mass (Da)
	P53152 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: E1DA713C94378795	FASTA	137	15,545
	10 20 30 40 50 60 MSKVPRNFRL LEELEKGEKG FGPESCSYGL ADSDDITMTK WNGTILGPPH SNHENRIYSL 70 80 90 100 110 120 SIDCGPNYPD SPPKVTFISK INLPCVNPTT GEVQTDFHTL RDWKRAYTME TLLLDLRKEM 130 ATPANKKLRQ PKEGETF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"MMS2, encoding a ubiquitin-conjugating-enzyme-like protein, is a member of the yeast error-free postreplication repair pathway." Broomfield S., Chow B.L., Xiao W. Proc. Natl. Acad. Sci. U.S.A. 95:5678-5683(1998) [PubMed: 9576943] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
[2]	"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII." Rieger M., Brueckner M., Schaefer M., Mueller-Auer S. Yeast 13:1077-1090(1997) [PubMed: 9290212] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"The products of the yeast MMS2 and two human homologs (hMMS2 and CROC-1) define a structurally and functionally conserved Ubc-like protein family." Xiao W., Lin S.L., Broomfield S., Chow B.L., Wei Y.-F. Nucleic Acids Res. 26:3908-3914(1998) [PubMed: 9705497] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Noncanonical MMS2-encoded ubiquitin-conjugating enzyme functions in assembly of novel polyubiquitin chains for DNA repair." Hofmann R.M., Pickart C.M. Cell 96:645-653(1999) [PubMed: 10089880] [Abstract] Cited for: CHARACTERIZATION.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-71, MASS SPECTROMETRY.
[9]	"Molecular insights into polyubiquitin chain assembly: crystal structure of the Mms2/Ubc13 heterodimer." VanDemark A.P., Hofmann R.M., Tsui C., Pickart C.M., Wolberger C. Cell 105:711-720(2001) [PubMed: 11440714] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) IN COMPLEX WITH UBC13, MUTAGENESIS OF PHE-8.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U66724 Genomic DNA. Translation: AAC24241.1.
Z72609 Genomic DNA. Translation: CAA96792.1.
BK006941 Genomic DNA. Translation: DAA08018.1.

PIR

S64094.

RefSeq

NP_011428.1. NM_001180952.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1JAT	X-ray	1.60	B	1-137	[»]
2GMI	X-ray	2.50	B	1-137	[»]

ProteinModelPortal

P53152.

SMR

P53152. Positions 3-137.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-5829N.

IntAct

P53152. 9 interactions.

MINT

MINT-580773.

STRING

P53152.

Proteomic databases

PeptideAtlas

P53152.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YGL087C; YGL087C; YGL087C.

GeneID

852793.

KEGG

sce:YGL087C.

NMPDR

fig|4932.3.peg.2533.

Organism-specific databases

CYGD

YGL087c.

SGD

S000003055. MMS2.

Phylogenomic databases

eggNOG

fuNOG09153.

GeneTree

EFGT00050000001511.

HOGENOM

HBG756483.

OMA

NINCINQ.

OrthoDB

EOG4WM84B.

Gene expression databases

ArrayExpress

P53152.

Genevestigator

P53152.

GermOnline

YGL087C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]

Gene3D

G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.

KO

K10704.

Pfam

PF00179. UQ_con. 1 hit.
[Graphical view]

SUPFAM

SSF54495. UBQ-conjugat/RWD-like. 1 hit.

PROSITE

PS00183. UBIQUITIN_CONJUGAT_1. False negative.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

972294.

Entry information

Entry name

MMS2_YEAST

Accession

Primary (citable) accession number: P53152
Secondary accession number(s): D6VU57

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program