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Protein

Ubiquitin-conjugating enzyme variant MMS2

Gene

MMS2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a role in the DNA error-free postreplication repair (PRR) pathway. Lacks catalytic activity by itself. The UBC13/MMS2 heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'.

GO - Molecular functioni

GO - Biological processi

  • free ubiquitin chain polymerization Source: SGD
  • postreplication repair Source: SGD
  • protein K63-linked ubiquitination Source: GO_Central
  • protein polyubiquitination Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-30588-MONOMER.
ReactomeiR-SCE-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme variant MMS2
Short name:
UEV MMS2
Gene namesi
Name:MMS2
Ordered Locus Names:YGL087C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL087C.
SGDiS000003055. MMS2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nucleus Source: SGD
  • ubiquitin conjugating enzyme complex Source: SGD
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi8F → A: Strongly reduces UBC13 binding and interferes with error-free DNA repair. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825981 – 137Ubiquitin-conjugating enzyme variant MMS2Add BLAST137

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei71PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53152.
PRIDEiP53152.

PTM databases

iPTMnetiP53152.

Interactioni

Subunit structurei

Heterodimer with UBC13.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
UBC13P524904EBI-11035,EBI-19777

GO - Molecular functioni

Protein-protein interaction databases

BioGridi33163. 132 interactors.
DIPiDIP-5829N.
IntActiP53152. 6 interactors.
MINTiMINT-580773.

Structurei

Secondary structure

1137
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 18Combined sources13
Beta strandi26 – 32Combined sources7
Beta strandi40 – 46Combined sources7
Beta strandi49 – 51Combined sources3
Turni52 – 55Combined sources4
Beta strandi57 – 63Combined sources7
Turni66 – 70Combined sources5
Beta strandi74 – 79Combined sources6
Beta strandi84 – 86Combined sources3
Turni88 – 90Combined sources3
Helixi98 – 101Combined sources4
Helixi109 – 120Combined sources12
Helixi123 – 126Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JATX-ray1.60B1-137[»]
2GMIX-ray2.50B1-137[»]
ProteinModelPortaliP53152.
SMRiP53152.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53152.

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00740000115534.
HOGENOMiHOG000036561.
InParanoidiP53152.
KOiK10704.
OMAiCFRTRIN.
OrthoDBiEOG092C4YVR.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53152-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKVPRNFRL LEELEKGEKG FGPESCSYGL ADSDDITMTK WNGTILGPPH
60 70 80 90 100
SNHENRIYSL SIDCGPNYPD SPPKVTFISK INLPCVNPTT GEVQTDFHTL
110 120 130
RDWKRAYTME TLLLDLRKEM ATPANKKLRQ PKEGETF
Length:137
Mass (Da):15,545
Last modified:October 1, 1996 - v1
Checksum:iE1DA713C94378795
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66724 Genomic DNA. Translation: AAC24241.1.
Z72609 Genomic DNA. Translation: CAA96792.1.
BK006941 Genomic DNA. Translation: DAA08018.1.
PIRiS64094.
RefSeqiNP_011428.1. NM_001180952.1.

Genome annotation databases

EnsemblFungiiYGL087C; YGL087C; YGL087C.
GeneIDi852793.
KEGGisce:YGL087C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66724 Genomic DNA. Translation: AAC24241.1.
Z72609 Genomic DNA. Translation: CAA96792.1.
BK006941 Genomic DNA. Translation: DAA08018.1.
PIRiS64094.
RefSeqiNP_011428.1. NM_001180952.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JATX-ray1.60B1-137[»]
2GMIX-ray2.50B1-137[»]
ProteinModelPortaliP53152.
SMRiP53152.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33163. 132 interactors.
DIPiDIP-5829N.
IntActiP53152. 6 interactors.
MINTiMINT-580773.

PTM databases

iPTMnetiP53152.

Proteomic databases

MaxQBiP53152.
PRIDEiP53152.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL087C; YGL087C; YGL087C.
GeneIDi852793.
KEGGisce:YGL087C.

Organism-specific databases

EuPathDBiFungiDB:YGL087C.
SGDiS000003055. MMS2.

Phylogenomic databases

GeneTreeiENSGT00740000115534.
HOGENOMiHOG000036561.
InParanoidiP53152.
KOiK10704.
OMAiCFRTRIN.
OrthoDBiEOG092C4YVR.

Enzyme and pathway databases

BioCyciYEAST:G3O-30588-MONOMER.
ReactomeiR-SCE-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.

Miscellaneous databases

EvolutionaryTraceiP53152.
PROiP53152.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMMS2_YEAST
AccessioniPrimary (citable) accession number: P53152
Secondary accession number(s): D6VU57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2760 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.