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Reviewed, UniProtKB/Swiss-Prot P53150 (LIF1_YEAST)

Last modified November 24, 2009. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Ligase-interacting factor 1
Gene names
Name: LIF1
Ordered Locus Names: YGL090W
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length421 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Stabilizes DNL4. Involved in non-homologous repair of DNA double-strand breaks. Ref.1

Subunit structure

Interacts with the BRCT domain of DNL4 and with NEJ1. The DNL4-LIF1 complex interacts with POL4. Ref.1 Ref.4 Ref.5

Subcellular location

Cytoplasm. Nucleus.

Miscellaneous

Present with 876 molecules/cell in log phase SD medium. Ref.7

Ontologies

Keywords
   Biological processDNA damage
DNA repair
   Cellular componentCytoplasm
Nucleus
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological processdouble-strand break repair via nonhomologous end joining Ref.1

Inferred from direct assay. Source: SGD

   Cellular componentDNA ligase IV complex

Inferred from physical interaction. Source: SGD

cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionchromatin binding

Inferred from direct assay. Source: SGD

protein binding Ref.4

Inferred from physical interaction. Source: IntAct

Complete GO annotation...

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 421421Ligase-interacting factor 1
PRO_0000084419

Amino acid modifications

Modified residue2611Phosphoserine Ref.8

Secondary structure

............ 421
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P53150-1 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 5BEDC72059E9996F

FASTA42148,259
        10         20         30         40         50         60 
MSQLTEFISC IPVVNEEQNE EDERGLCKIQ IEDGAMLETL DENSLSGLRI EKMLVSEGTG 

        70         80         90        100        110        120 
IFSKSSFGIN DLRIFTGENI DEESKKYVWY ELLKMLTGHK VYIASLDEKV VFTKWTCRMQ 

       130        140        150        160        170        180 
DDEVWKVVME LESSAIIRKI AELTLHPVKK GEIDLFEMAD KLYKDICCVN DSYRNIKESD 

       190        200        210        220        230        240 
SSNRNRVEQL ARERELLDKL LETRDERTRA MMVTLLNEKK KKIRELHEIL RQNNIKLSDD 

       250        260        270        280        290        300 
DVLDSALINT EVQKPISELN SPGKRMKRRK TVVEPQNLQK KLKDTSRRRA NRKISNQSVI 

       310        320        330        340        350        360 
KMEDDDFDDF QFFGLSKRPI ITAKDKLSEK YDDITSFGDD TQSISFESDS SSDVQKHLVS 

       370        380        390        400        410        420 
LEDNGIQISA GRSDEDYGDI SGSESETDAS AGEKKSSNHS EQSGNDREPC LQTESETDIE 


T 

« Hide

References

« Hide 'large scale' references
[1]"Saccharomyces cerevisiae LIF1: a function involved in DNA double-strand break repair related to mammalian XRCC4."
Herrmann G., Lindahl T., Schar P.
EMBO J. 17:4188-4198(1998) [PubMed: 9670033] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH DNL4.
[2]"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
Yeast 13:1077-1090(1997) [PubMed: 9290212] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed: 9169869] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[4]"NHEJ regulation by mating type is exercised through a novel protein, Lif2p, essential to the ligase IV pathway."
Frank-Vaillant M., Marcand S.
Genes Dev. 15:3005-3012(2001) [PubMed: 11711435] [Abstract]
Cited for: INTERACTION WITH NEJ1.
[5]"A physical and functional interaction between yeast Pol4 and Dnl4-Lif1 links DNA synthesis and ligation in nonhomologous end joining."
Tseng H.-M., Tomkinson A.E.
J. Biol. Chem. 277:45630-45637(2002) [PubMed: 12235149] [Abstract]
Cited for: INTERACTION OF THE DNL4-LIF1 COMPLEX WITH POL4.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed: 14562095] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases."
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-261, MASS SPECTROMETRY.
[9]"Structure of an Xrcc4-DNA ligase IV yeast ortholog complex reveals a novel BRCT interaction mode."
Dore A.S., Furnham N., Davies O.R., Sibanda B.L., Chirgadze D.Y., Jackson S.P., Pellegrini L., Blundell T.L.
DNA Repair 5:362-368(2006) [PubMed: 16388993] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.92 ANGSTROMS) OF 1-246 IN COMPLEX WITH THE TANDEM BRCT DOMAINS OF DNL4.
+Additional computationally mapped references.

Cross-references

Sequence databases

Z72612 Genomic DNA. Translation: CAA96796.1.
PIRS64097.
RefSeqNP_011425.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1Z56X-ray3.92A/B1-246[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:5477N.
IntActP53150. 11 interactions.
STRINGP53150.

Proteomic databases

PeptideAtlasP53150.
PRIDEP53150.

Genome annotation databases

EnsemblYGL090W; YGL090W; YGL090W; Saccharomyces cerevisiae. [Genome view]
GeneID852790.
KEGGsce:YGL090W.
NMPDRfig|4932.3.peg.2530.

Organism-specific databases

CYGDYGL090w.
SGDS000003058. LIF1.

Phylogenomic databases

OrthoDBEOG9S1VQQ

Gene expression databases

ArrayExpressP53150.
GenevestigatorP53150.
GermOnlineYGL090W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR015381. XLF/Cernunnos.
[Graphical view]
PfamPF09302. XLF. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio972288.

Entry information

Entry nameLIF1_YEAST
AccessionPrimary (citable) accession number: P53150
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 24, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents