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Protein

Ligase-interacting factor 1

Gene

LIF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Stabilizes DNL4. Involved in non-homologous repair of DNA double-strand breaks.1 Publication

GO - Molecular functioni

  • chromatin binding Source: SGD
  • DNA binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair

Enzyme and pathway databases

BioCyciYEAST:G3O-30590-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ligase-interacting factor 1
Gene namesi
Name:LIF1
Ordered Locus Names:YGL090W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL090W.
SGDiS000003058. LIF1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • DNA ligase IV complex Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 421421Ligase-interacting factor 1PRO_0000084419Add
BLAST

Proteomic databases

MaxQBiP53150.

PTM databases

iPTMnetiP53150.

Interactioni

Subunit structurei

Interacts with the BRCT domain of DNL4 and with NEJ1. The DNL4-LIF1 complex interacts with POL4.4 Publications

Protein-protein interaction databases

BioGridi33161. 31 interactions.
DIPiDIP-5477N.
IntActiP53150. 6 interactions.
MINTiMINT-492607.

Structurei

Secondary structure

1
421
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi163 – 1675
Helixi168 – 1758
Turni176 – 1816
Helixi182 – 1898
Turni190 – 1934
Helixi194 – 20310
Turni204 – 2063
Helixi207 – 22519
Helixi226 – 2283
Turni229 – 2335

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Z56X-ray3.92A/B1-246[»]
ProteinModelPortaliP53150.
SMRiP53150. Positions 159-235.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53150.

Family & Domainsi

Phylogenomic databases

InParanoidiP53150.
KOiK10982.
OMAiASHCELN.
OrthoDBiEOG092C2JP3.

Family and domain databases

Gene3Di1.20.5.370. 1 hit.
InterProiIPR015381. XLF_fam.
IPR014751. XRCC4_C.
[Graphical view]
PfamiPF09302. XLF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53150-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQLTEFISC IPVVNEEQNE EDERGLCKIQ IEDGAMLETL DENSLSGLRI
60 70 80 90 100
EKMLVSEGTG IFSKSSFGIN DLRIFTGENI DEESKKYVWY ELLKMLTGHK
110 120 130 140 150
VYIASLDEKV VFTKWTCRMQ DDEVWKVVME LESSAIIRKI AELTLHPVKK
160 170 180 190 200
GEIDLFEMAD KLYKDICCVN DSYRNIKESD SSNRNRVEQL ARERELLDKL
210 220 230 240 250
LETRDERTRA MMVTLLNEKK KKIRELHEIL RQNNIKLSDD DVLDSALINT
260 270 280 290 300
EVQKPISELN SPGKRMKRRK TVVEPQNLQK KLKDTSRRRA NRKISNQSVI
310 320 330 340 350
KMEDDDFDDF QFFGLSKRPI ITAKDKLSEK YDDITSFGDD TQSISFESDS
360 370 380 390 400
SSDVQKHLVS LEDNGIQISA GRSDEDYGDI SGSESETDAS AGEKKSSNHS
410 420
EQSGNDREPC LQTESETDIE T
Length:421
Mass (Da):48,259
Last modified:October 1, 1996 - v1
Checksum:i5BEDC72059E9996F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti44 – 441S → G in AAU09724 (PubMed:17322287).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72612 Genomic DNA. Translation: CAA96796.1.
AY723807 Genomic DNA. Translation: AAU09724.1.
BK006941 Genomic DNA. Translation: DAA08016.1.
PIRiS64097.
RefSeqiNP_011425.1. NM_001180955.1.

Genome annotation databases

EnsemblFungiiYGL090W; YGL090W; YGL090W.
GeneIDi852790.
KEGGisce:YGL090W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72612 Genomic DNA. Translation: CAA96796.1.
AY723807 Genomic DNA. Translation: AAU09724.1.
BK006941 Genomic DNA. Translation: DAA08016.1.
PIRiS64097.
RefSeqiNP_011425.1. NM_001180955.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Z56X-ray3.92A/B1-246[»]
ProteinModelPortaliP53150.
SMRiP53150. Positions 159-235.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33161. 31 interactions.
DIPiDIP-5477N.
IntActiP53150. 6 interactions.
MINTiMINT-492607.

PTM databases

iPTMnetiP53150.

Proteomic databases

MaxQBiP53150.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL090W; YGL090W; YGL090W.
GeneIDi852790.
KEGGisce:YGL090W.

Organism-specific databases

EuPathDBiFungiDB:YGL090W.
SGDiS000003058. LIF1.

Phylogenomic databases

InParanoidiP53150.
KOiK10982.
OMAiASHCELN.
OrthoDBiEOG092C2JP3.

Enzyme and pathway databases

BioCyciYEAST:G3O-30590-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP53150.
PROiP53150.

Family and domain databases

Gene3Di1.20.5.370. 1 hit.
InterProiIPR015381. XLF_fam.
IPR014751. XRCC4_C.
[Graphical view]
PfamiPF09302. XLF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLIF1_YEAST
AccessioniPrimary (citable) accession number: P53150
Secondary accession number(s): D6VU55, E9P953
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 876 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.