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P53150 (LIF1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ligase-interacting factor 1
Gene names
Name:LIF1
Ordered Locus Names:YGL090W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length421 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Stabilizes DNL4. Involved in non-homologous repair of DNA double-strand breaks. Ref.1

Subunit structure

Interacts with the BRCT domain of DNL4 and with NEJ1. The DNL4-LIF1 complex interacts with POL4. Ref.1 Ref.6 Ref.7

Subcellular location

Cytoplasm. Nucleus Ref.8.

Miscellaneous

Present with 876 molecules/cell in log phase SD medium.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 421421Ligase-interacting factor 1
PRO_0000084419

Experimental info

Sequence conflict441S → G in AAU09724. Ref.5

Secondary structure

............ 421
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P53150 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 5BEDC72059E9996F

FASTA42148,259
        10         20         30         40         50         60 
MSQLTEFISC IPVVNEEQNE EDERGLCKIQ IEDGAMLETL DENSLSGLRI EKMLVSEGTG 

        70         80         90        100        110        120 
IFSKSSFGIN DLRIFTGENI DEESKKYVWY ELLKMLTGHK VYIASLDEKV VFTKWTCRMQ 

       130        140        150        160        170        180 
DDEVWKVVME LESSAIIRKI AELTLHPVKK GEIDLFEMAD KLYKDICCVN DSYRNIKESD 

       190        200        210        220        230        240 
SSNRNRVEQL ARERELLDKL LETRDERTRA MMVTLLNEKK KKIRELHEIL RQNNIKLSDD 

       250        260        270        280        290        300 
DVLDSALINT EVQKPISELN SPGKRMKRRK TVVEPQNLQK KLKDTSRRRA NRKISNQSVI 

       310        320        330        340        350        360 
KMEDDDFDDF QFFGLSKRPI ITAKDKLSEK YDDITSFGDD TQSISFESDS SSDVQKHLVS 

       370        380        390        400        410        420 
LEDNGIQISA GRSDEDYGDI SGSESETDAS AGEKKSSNHS EQSGNDREPC LQTESETDIE 


T 

« Hide

References

« Hide 'large scale' references
[1]"Saccharomyces cerevisiae LIF1: a function involved in DNA double-strand break repair related to mammalian XRCC4."
Herrmann G., Lindahl T., Schar P.
EMBO J. 17:4188-4198(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH DNL4.
[2]"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"NHEJ regulation by mating type is exercised through a novel protein, Lif2p, essential to the ligase IV pathway."
Frank-Vaillant M., Marcand S.
Genes Dev. 15:3005-3012(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NEJ1.
[7]"A physical and functional interaction between yeast Pol4 and Dnl4-Lif1 links DNA synthesis and ligation in nonhomologous end joining."
Tseng H.-M., Tomkinson A.E.
J. Biol. Chem. 277:45630-45637(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION OF THE DNL4-LIF1 COMPLEX WITH POL4.
[8]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Structure of an Xrcc4-DNA ligase IV yeast ortholog complex reveals a novel BRCT interaction mode."
Dore A.S., Furnham N., Davies O.R., Sibanda B.L., Chirgadze D.Y., Jackson S.P., Pellegrini L., Blundell T.L.
DNA Repair 5:362-368(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.92 ANGSTROMS) OF 1-246 IN COMPLEX WITH THE TANDEM BRCT DOMAINS OF DNL4.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z72612 Genomic DNA. Translation: CAA96796.1.
AY723807 Genomic DNA. Translation: AAU09724.1.
BK006941 Genomic DNA. Translation: DAA08016.1.
PIRS64097.
RefSeqNP_011425.1. NM_001180955.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1Z56X-ray3.92A/B1-246[»]
ProteinModelPortalP53150.
SMRP53150. Positions 159-235.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33161. 28 interactions.
DIPDIP-5477N.
IntActP53150. 6 interactions.
MINTMINT-492607.
STRING4932.YGL090W.

Proteomic databases

PaxDbP53150.
PeptideAtlasP53150.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGL090W; YGL090W; YGL090W.
GeneID852790.
KEGGsce:YGL090W.

Organism-specific databases

CYGDYGL090w.
SGDS000003058. LIF1.

Phylogenomic databases

eggNOGNOG72265.
KOK10982.
OrthoDBEOG7CNZR9.

Enzyme and pathway databases

BioCycYEAST:G3O-30590-MONOMER.

Gene expression databases

GenevestigatorP53150.

Family and domain databases

Gene3D1.20.5.370. 1 hit.
InterProIPR015381. XLF/Cernunnos.
IPR014751. XRCC4_C.
[Graphical view]
PfamPF09302. XLF. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP53150.
NextBio972288.

Entry information

Entry nameLIF1_YEAST
AccessionPrimary (citable) accession number: P53150
Secondary accession number(s): D6VU55, E9P953
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

PDB cross-references

Index of Protein Data Bank (PDB) cross-references