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P53150

- LIF1_YEAST

UniProt

P53150 - LIF1_YEAST

Protein

Ligase-interacting factor 1

Gene

LIF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Stabilizes DNL4. Involved in non-homologous repair of DNA double-strand breaks.1 Publication

    GO - Molecular functioni

    1. chromatin binding Source: SGD
    2. DNA binding Source: InterPro

    GO - Biological processi

    1. DNA recombination Source: InterPro
    2. double-strand break repair via nonhomologous end joining Source: SGD

    Keywords - Biological processi

    DNA damage, DNA repair

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30590-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ligase-interacting factor 1
    Gene namesi
    Name:LIF1
    Ordered Locus Names:YGL090W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGL090w.
    SGDiS000003058. LIF1.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. DNA ligase IV complex Source: SGD
    3. nucleus Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 421421Ligase-interacting factor 1PRO_0000084419Add
    BLAST

    Proteomic databases

    MaxQBiP53150.
    PaxDbiP53150.
    PeptideAtlasiP53150.

    Expressioni

    Gene expression databases

    GenevestigatoriP53150.

    Interactioni

    Subunit structurei

    Interacts with the BRCT domain of DNL4 and with NEJ1. The DNL4-LIF1 complex interacts with POL4.4 Publications

    Protein-protein interaction databases

    BioGridi33161. 28 interactions.
    DIPiDIP-5477N.
    IntActiP53150. 6 interactions.
    MINTiMINT-492607.
    STRINGi4932.YGL090W.

    Structurei

    Secondary structure

    1
    421
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi163 – 1675
    Helixi168 – 1758
    Turni176 – 1816
    Helixi182 – 1898
    Turni190 – 1934
    Helixi194 – 20310
    Turni204 – 2063
    Helixi207 – 22519
    Helixi226 – 2283
    Turni229 – 2335

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1Z56X-ray3.92A/B1-246[»]
    ProteinModelPortaliP53150.
    SMRiP53150. Positions 159-235.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP53150.

    Family & Domainsi

    Phylogenomic databases

    eggNOGiNOG72265.
    KOiK10982.
    OrthoDBiEOG7CNZR9.

    Family and domain databases

    Gene3Di1.20.5.370. 1 hit.
    InterProiIPR015381. XLF/Cernunnos.
    IPR014751. XRCC4_C.
    [Graphical view]
    PfamiPF09302. XLF. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53150-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQLTEFISC IPVVNEEQNE EDERGLCKIQ IEDGAMLETL DENSLSGLRI    50
    EKMLVSEGTG IFSKSSFGIN DLRIFTGENI DEESKKYVWY ELLKMLTGHK 100
    VYIASLDEKV VFTKWTCRMQ DDEVWKVVME LESSAIIRKI AELTLHPVKK 150
    GEIDLFEMAD KLYKDICCVN DSYRNIKESD SSNRNRVEQL ARERELLDKL 200
    LETRDERTRA MMVTLLNEKK KKIRELHEIL RQNNIKLSDD DVLDSALINT 250
    EVQKPISELN SPGKRMKRRK TVVEPQNLQK KLKDTSRRRA NRKISNQSVI 300
    KMEDDDFDDF QFFGLSKRPI ITAKDKLSEK YDDITSFGDD TQSISFESDS 350
    SSDVQKHLVS LEDNGIQISA GRSDEDYGDI SGSESETDAS AGEKKSSNHS 400
    EQSGNDREPC LQTESETDIE T 421
    Length:421
    Mass (Da):48,259
    Last modified:October 1, 1996 - v1
    Checksum:i5BEDC72059E9996F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti44 – 441S → G in AAU09724. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72612 Genomic DNA. Translation: CAA96796.1.
    AY723807 Genomic DNA. Translation: AAU09724.1.
    BK006941 Genomic DNA. Translation: DAA08016.1.
    PIRiS64097.
    RefSeqiNP_011425.1. NM_001180955.1.

    Genome annotation databases

    EnsemblFungiiYGL090W; YGL090W; YGL090W.
    GeneIDi852790.
    KEGGisce:YGL090W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72612 Genomic DNA. Translation: CAA96796.1 .
    AY723807 Genomic DNA. Translation: AAU09724.1 .
    BK006941 Genomic DNA. Translation: DAA08016.1 .
    PIRi S64097.
    RefSeqi NP_011425.1. NM_001180955.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1Z56 X-ray 3.92 A/B 1-246 [» ]
    ProteinModelPortali P53150.
    SMRi P53150. Positions 159-235.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33161. 28 interactions.
    DIPi DIP-5477N.
    IntActi P53150. 6 interactions.
    MINTi MINT-492607.
    STRINGi 4932.YGL090W.

    Proteomic databases

    MaxQBi P53150.
    PaxDbi P53150.
    PeptideAtlasi P53150.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL090W ; YGL090W ; YGL090W .
    GeneIDi 852790.
    KEGGi sce:YGL090W.

    Organism-specific databases

    CYGDi YGL090w.
    SGDi S000003058. LIF1.

    Phylogenomic databases

    eggNOGi NOG72265.
    KOi K10982.
    OrthoDBi EOG7CNZR9.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30590-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P53150.
    NextBioi 972288.

    Gene expression databases

    Genevestigatori P53150.

    Family and domain databases

    Gene3Di 1.20.5.370. 1 hit.
    InterProi IPR015381. XLF/Cernunnos.
    IPR014751. XRCC4_C.
    [Graphical view ]
    Pfami PF09302. XLF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Saccharomyces cerevisiae LIF1: a function involved in DNA double-strand break repair related to mammalian XRCC4."
      Herrmann G., Lindahl T., Schar P.
      EMBO J. 17:4188-4198(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH DNL4.
    2. "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
      Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
      Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "NHEJ regulation by mating type is exercised through a novel protein, Lif2p, essential to the ligase IV pathway."
      Frank-Vaillant M., Marcand S.
      Genes Dev. 15:3005-3012(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NEJ1.
    7. "A physical and functional interaction between yeast Pol4 and Dnl4-Lif1 links DNA synthesis and ligation in nonhomologous end joining."
      Tseng H.-M., Tomkinson A.E.
      J. Biol. Chem. 277:45630-45637(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION OF THE DNL4-LIF1 COMPLEX WITH POL4.
    8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Structure of an Xrcc4-DNA ligase IV yeast ortholog complex reveals a novel BRCT interaction mode."
      Dore A.S., Furnham N., Davies O.R., Sibanda B.L., Chirgadze D.Y., Jackson S.P., Pellegrini L., Blundell T.L.
      DNA Repair 5:362-368(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.92 ANGSTROMS) OF 1-246 IN COMPLEX WITH THE TANDEM BRCT DOMAINS OF DNL4.

    Entry informationi

    Entry nameiLIF1_YEAST
    AccessioniPrimary (citable) accession number: P53150
    Secondary accession number(s): D6VU55, E9P953
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 876 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3