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Protein

Spindle pole body component SPC105

Gene

SPC105

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Forms a kinetochore complex with SPC105 which is required for kinetochore binding by a discrete subset of kMAPs (BIM1, BIK1 and SLK19) and motors (CIN8, KAR3). Involved in kinetochore-microtubule binding and the spindle assembly checkpoint.1 Publication

GO - Molecular functioni

  • microtubule binding Source: SGD
  • structural constituent of cytoskeleton Source: SGD

GO - Biological processi

  • microtubule nucleation Source: SGD
  • mitotic spindle assembly checkpoint Source: SGD
  • protein localization to kinetochore Source: SGD
  • sister chromatid biorientation Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-30593-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Spindle pole body component SPC105
Alternative name(s):
105 kDa spindle pole component protein
Gene namesi
Name:SPC105
Ordered Locus Names:YGL093W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL093W.
SGDiS000003061. SPC105.

Subcellular locationi

GO - Cellular componenti

  • condensed nuclear chromosome kinetochore Source: SGD
  • cytoplasm Source: UniProtKB-KW
  • nuclear membrane Source: UniProtKB-SubCell
  • spindle pole body Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 917917Spindle pole body component SPC105PRO_0000202755Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei77 – 771PhosphoserineCombined sources
Modified residuei356 – 3561PhosphothreonineCombined sources
Modified residuei380 – 3801PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53148.
PRIDEiP53148.

PTM databases

iPTMnetiP53148.

Interactioni

Subunit structurei

Component of the SPC105 complex composed of at least SPC105 and KRE28. The SPC105 complex interacts with the MIND and NDC80 complexes at the centromere.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
KRE28Q044313EBI-23870,EBI-32446
NDC80P404602EBI-23870,EBI-25247

GO - Molecular functioni

  • microtubule binding Source: SGD

Protein-protein interaction databases

BioGridi33158. 75 interactions.
DIPiDIP-3019N.
IntActiP53148. 10 interactions.
MINTiMINT-434703.

Structurei

Secondary structure

1
917
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi170 – 1723Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4BL0X-ray1.95C/F165-183[»]
ProteinModelPortaliP53148.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

InParanoidiP53148.
KOiK11563.
OMAiKVWFEWR.
OrthoDBiEOG741Z9G.

Family and domain databases

InterProiIPR033338. Spc105/Spc7.
IPR013253. Spc7_domain.
[Graphical view]
PANTHERiPTHR28260. PTHR28260. 1 hit.
PfamiPF08317. Spc7. 1 hit.
[Graphical view]
SMARTiSM00787. Spc7. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53148-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVDERSRIG GREKDAGPGK GILKQNQSSQ MTSSFLENPG VRIPTRIITK
60 70 80 90 100
KEVLDGSNTT SRINTSNLQS MVKRRVSFAP DVTLHSFTFV PEQNNEIKEP
110 120 130 140 150
RRRKTSTNSP TKISSQEEPL VTSTQIDDAR TEEKTAAEED PDTSGMELTE
160 170 180 190 200
PIVATPDSNK ASQHDPTSME MTEVFPRSIR QKNPDVEGES IESSQQIDDV
210 220 230 240 250
EAVREETMEL TAIHNVHDYD SISKDTVEGE PIDLTEYESK PYVPNSVSRS
260 270 280 290 300
TGKSSDYSVE RSNDKSDLSK SENKTNSSQP MEITDIFHAD PQNPMSLHSD
310 320 330 340 350
NNINNDGNEM ELTQIQTNFD RDNHHIDESP SEKHAFSSNK RRKLDTVSDY
360 370 380 390 400
AASVTTPVKE AKDTSGEDND GDLEMMEKMS PITFSDVDNK IGTRSNDVFT
410 420 430 440 450
IEPGTEDTGM QTATDDEEDG ENVDDNGNKI VEKTRLPEID KEGQSGIALP
460 470 480 490 500
TQDYTLREFI NEVGVGFLDT KLIDDLDKKV NFPLNSFNFV ENQRIDNVFS
510 520 530 540 550
AFYIDIPILE VEAFRCKELW RSINESKDKF KDFEAQIDKS HPPLLLQEYF
560 570 580 590 600
SSDEKMKQLM RDQLQLVKGY SKLEAAMEWY EWRKKQLNGL ELILAENLNT
610 620 630 640 650
LKREYEKLNE EVEKVNSIRG KIRKLNEAIK EEIRSLKNLP SDSYKPTLMN
660 670 680 690 700
RIKIEAFKQE LMEHSISLSS SNDFTQEMRS LKLAIAKKSN DILTLRSEVA
710 720 730 740 750
SIDKKIEKRK LFTRFDLPKL RDTLKILESL TGVRFLKFSK ATLSIAFLQL
760 770 780 790 800
DDLRVDINLA NFKNNPLSSM KVMNDSNNDD MSYHLFTMLL KNVEAEHQDS
810 820 830 840 850
MLSNLFFAMK KWRPLLKYIK LLKLLFPVKI TQTEEEEALL QFKDYDRRNK
860 870 880 890 900
TAFFYVISLV SFAQGVFSEN GQIPMKVHIS TQQDYSPSRE VLSDRITHKI
910
SGVLPSFTKS RIHLEFT
Length:917
Mass (Da):104,826
Last modified:October 1, 1996 - v1
Checksum:i8B7ED1522916A319
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72615 Genomic DNA. Translation: CAA96799.1.
BK006941 Genomic DNA. Translation: DAA08013.1.
PIRiS64100.
RefSeqiNP_011422.1. NM_001180958.1.

Genome annotation databases

EnsemblFungiiYGL093W; YGL093W; YGL093W.
GeneIDi852787.
KEGGisce:YGL093W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72615 Genomic DNA. Translation: CAA96799.1.
BK006941 Genomic DNA. Translation: DAA08013.1.
PIRiS64100.
RefSeqiNP_011422.1. NM_001180958.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4BL0X-ray1.95C/F165-183[»]
ProteinModelPortaliP53148.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33158. 75 interactions.
DIPiDIP-3019N.
IntActiP53148. 10 interactions.
MINTiMINT-434703.

PTM databases

iPTMnetiP53148.

Proteomic databases

MaxQBiP53148.
PRIDEiP53148.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL093W; YGL093W; YGL093W.
GeneIDi852787.
KEGGisce:YGL093W.

Organism-specific databases

EuPathDBiFungiDB:YGL093W.
SGDiS000003061. SPC105.

Phylogenomic databases

InParanoidiP53148.
KOiK11563.
OMAiKVWFEWR.
OrthoDBiEOG741Z9G.

Enzyme and pathway databases

BioCyciYEAST:G3O-30593-MONOMER.

Miscellaneous databases

NextBioi972279.
PROiP53148.

Family and domain databases

InterProiIPR033338. Spc105/Spc7.
IPR013253. Spc7_domain.
[Graphical view]
PANTHERiPTHR28260. PTHR28260. 1 hit.
PfamiPF08317. Spc7. 1 hit.
[Graphical view]
SMARTiSM00787. Spc7. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
    Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
    Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Analysis of the Saccharomyces spindle pole by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry."
    Wigge P.A., Jensen O.N., Holmes S., Soues S., Mann M., Kilmartin J.V.
    J. Cell Biol. 141:967-977(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT, SUBCELLULAR LOCATION.
  5. "Interactions between centromere complexes in Saccharomyces cerevisiae."
    Nekrasov V.S., Smith M.A., Peak-Chew S., Kilmartin J.V.
    Mol. Biol. Cell 14:4931-4946(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, INTERACTION WITH KRE28, SUBCELLULAR LOCATION.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. "Molecular architecture of a kinetochore-microtubule attachment site."
    Joglekar A.P., Bouck D.C., Molk J.N., Bloom K.S., Salmon E.D.
    Nat. Cell Biol. 8:581-585(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, SUBCELLULAR LOCATION.
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-356 AND SER-380, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-380, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Roles for the conserved spc105p/kre28p complex in kinetochore-microtubule binding and the spindle assembly checkpoint."
    Pagliuca C., Draviam V.M., Marco E., Sorger P.K., De Wulf P.
    PLoS ONE 4:E7640-E7640(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH KRE28, FUNCTION.
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77; THR-356 AND SER-380, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSP105_YEAST
AccessioniPrimary (citable) accession number: P53148
Secondary accession number(s): D6VU52
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 11, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.