Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Spindle pole body component SPC105

Gene

SPC105

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Forms a kinetochore complex with SPC105 which is required for kinetochore binding by a discrete subset of kMAPs (BIM1, BIK1 and SLK19) and motors (CIN8, KAR3). Involved in kinetochore-microtubule binding and the spindle assembly checkpoint.1 Publication

GO - Molecular functioni

  • microtubule binding Source: SGD
  • microtubule plus-end binding Source: GO_Central
  • structural constituent of cytoskeleton Source: SGD

GO - Biological processi

  • microtubule nucleation Source: SGD
  • mitotic sister chromatid biorientation Source: GO_Central
  • mitotic spindle assembly checkpoint Source: SGD
  • protein localization to kinetochore Source: SGD
  • sister chromatid biorientation Source: SGD

Enzyme and pathway databases

BioCyciYEAST:G3O-30593-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Spindle pole body component SPC105
Alternative name(s):
105 kDa spindle pole component protein
Gene namesi
Name:SPC105
Ordered Locus Names:YGL093W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL093W.
SGDiS000003061. SPC105.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002027551 – 917Spindle pole body component SPC105Add BLAST917

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei77PhosphoserineCombined sources1
Modified residuei356PhosphothreonineCombined sources1
Modified residuei380PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53148.
PRIDEiP53148.

PTM databases

iPTMnetiP53148.

Interactioni

Subunit structurei

Component of the SPC105 complex composed of at least SPC105 and KRE28. The SPC105 complex interacts with the MIND and NDC80 complexes at the centromere.4 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • microtubule binding Source: SGD
  • microtubule plus-end binding Source: GO_Central

Protein-protein interaction databases

BioGridi33158. 354 interactors.
DIPiDIP-3019N.
IntActiP53148. 10 interactors.
MINTiMINT-434703.
STRINGi4932.YGL093W.

Structurei

Secondary structure

1917
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi170 – 172Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4BL0X-ray1.95C/F165-183[»]
ProteinModelPortaliP53148.
SMRiP53148.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

InParanoidiP53148.
KOiK11563.
OMAiMQITQEI.
OrthoDBiEOG092C001Z.

Family and domain databases

InterProiView protein in InterPro
IPR033338. Spc105/Spc7.
IPR013253. Spc7_domain.
PANTHERiPTHR28260. PTHR28260. 2 hits.
PfamiView protein in Pfam
PF08317. Spc7. 1 hit.
SMARTiView protein in SMART
SM00787. Spc7. 1 hit.

Sequencei

Sequence statusi: Complete.

P53148-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVDERSRIG GREKDAGPGK GILKQNQSSQ MTSSFLENPG VRIPTRIITK
60 70 80 90 100
KEVLDGSNTT SRINTSNLQS MVKRRVSFAP DVTLHSFTFV PEQNNEIKEP
110 120 130 140 150
RRRKTSTNSP TKISSQEEPL VTSTQIDDAR TEEKTAAEED PDTSGMELTE
160 170 180 190 200
PIVATPDSNK ASQHDPTSME MTEVFPRSIR QKNPDVEGES IESSQQIDDV
210 220 230 240 250
EAVREETMEL TAIHNVHDYD SISKDTVEGE PIDLTEYESK PYVPNSVSRS
260 270 280 290 300
TGKSSDYSVE RSNDKSDLSK SENKTNSSQP MEITDIFHAD PQNPMSLHSD
310 320 330 340 350
NNINNDGNEM ELTQIQTNFD RDNHHIDESP SEKHAFSSNK RRKLDTVSDY
360 370 380 390 400
AASVTTPVKE AKDTSGEDND GDLEMMEKMS PITFSDVDNK IGTRSNDVFT
410 420 430 440 450
IEPGTEDTGM QTATDDEEDG ENVDDNGNKI VEKTRLPEID KEGQSGIALP
460 470 480 490 500
TQDYTLREFI NEVGVGFLDT KLIDDLDKKV NFPLNSFNFV ENQRIDNVFS
510 520 530 540 550
AFYIDIPILE VEAFRCKELW RSINESKDKF KDFEAQIDKS HPPLLLQEYF
560 570 580 590 600
SSDEKMKQLM RDQLQLVKGY SKLEAAMEWY EWRKKQLNGL ELILAENLNT
610 620 630 640 650
LKREYEKLNE EVEKVNSIRG KIRKLNEAIK EEIRSLKNLP SDSYKPTLMN
660 670 680 690 700
RIKIEAFKQE LMEHSISLSS SNDFTQEMRS LKLAIAKKSN DILTLRSEVA
710 720 730 740 750
SIDKKIEKRK LFTRFDLPKL RDTLKILESL TGVRFLKFSK ATLSIAFLQL
760 770 780 790 800
DDLRVDINLA NFKNNPLSSM KVMNDSNNDD MSYHLFTMLL KNVEAEHQDS
810 820 830 840 850
MLSNLFFAMK KWRPLLKYIK LLKLLFPVKI TQTEEEEALL QFKDYDRRNK
860 870 880 890 900
TAFFYVISLV SFAQGVFSEN GQIPMKVHIS TQQDYSPSRE VLSDRITHKI
910
SGVLPSFTKS RIHLEFT
Length:917
Mass (Da):104,826
Last modified:October 1, 1996 - v1
Checksum:i8B7ED1522916A319
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72615 Genomic DNA. Translation: CAA96799.1.
BK006941 Genomic DNA. Translation: DAA08013.1.
PIRiS64100.
RefSeqiNP_011422.1. NM_001180958.1.

Genome annotation databases

EnsemblFungiiYGL093W; YGL093W; YGL093W.
GeneIDi852787.
KEGGisce:YGL093W.

Similar proteinsi

Entry informationi

Entry nameiSP105_YEAST
AccessioniPrimary (citable) accession number: P53148
Secondary accession number(s): D6VU52
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 22, 2017
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names