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Protein

Protein transport protein USE1

Gene

USE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

SNARE required for targeting and fusion of Golgi-derived retrograde transport vesicles with the ER.4 Publications

GO - Molecular functioni

  • SNAP receptor activity Source: SGD

GO - Biological processi

  • protein transport Source: UniProtKB-KW
  • retrograde vesicle-mediated transport, Golgi to ER Source: SGD
  • vesicle fusion with endoplasmic reticulum Source: SGD
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30598-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein transport protein USE1
Alternative name(s):
SNARE-like tail-anchored protein 1
Unconventional SNARE in the endoplasmic reticulum protein 1
Gene namesi
Name:USE1
Synonyms:SLT1
Ordered Locus Names:YGL098W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL098W.
SGDiS000003066. USE1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 218218CytoplasmicSequence analysisAdd
BLAST
Transmembranei219 – 23921Helical; Anchor for type IV membrane proteinSequence analysisAdd
BLAST
Topological domaini240 – 2456LumenalSequence analysis

GO - Cellular componenti

  • integral component of cytoplasmic side of endoplasmic reticulum membrane Source: SGD
  • SNARE complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi183 – 1831D → G in USE1-0; slows down protein transport from the endoplasmic reticulum to the Golgi at 37 degrees Celsius. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 245245Protein transport protein USE1PRO_0000215584Add
BLAST

Proteomic databases

MaxQBiP53146.

Interactioni

Subunit structurei

Component of a SNARE complex consisting of UFE1, USE1, SEC20 and SEC22 or YKT6.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SEC20P287914EBI-23881,EBI-16572
SEC39Q127453EBI-23881,EBI-31898
UFE1P418342EBI-23881,EBI-20016

GO - Molecular functioni

  • SNAP receptor activity Source: SGD

Protein-protein interaction databases

BioGridi33151. 71 interactions.
DIPiDIP-5478N.
IntActiP53146. 9 interactions.
MINTiMINT-475168.

Structurei

3D structure databases

ProteinModelPortaliP53146.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the USE1 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000000925.
InParanoidiP53146.
KOiK08507.
OMAiDRQIEDQ.
OrthoDBiEOG092C5D1S.

Family and domain databases

InterProiIPR019150. Vesicle_transport_protein_Use1.
[Graphical view]
PfamiPF09753. Use1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53146-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAETSNDPFL SYVLSSKQLT NLNRLRRKAV TKQLGSSDDN KVSEEFLRYQ
60 70 80 90 100
HTYQREAFEY LQTKHDAHKI MESQYEQYQS SSKTRRYSID LDSVDAVDTE
110 120 130 140 150
SQTEYPNEEF IDRNEDSEAV MELRKRLLGK GQNKGLGYET TKSVDRQIED
160 170 180 190 200
QDTLQQDLIQ DMSKLVGSLK QGAVAFQSAL DEDKQVLGAA EIGIQVASQG
210 220 230 240
LMDVSGKLRK YDKSKLSYLF YITVFIFMIL GLVFTFIIIQ LFPAL
Length:245
Mass (Da):28,107
Last modified:October 1, 1996 - v1
Checksum:i213ABBF0492E80EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72620 Genomic DNA. Translation: CAA96804.1.
AY557814 Genomic DNA. Translation: AAS56140.1.
BK006941 Genomic DNA. Translation: DAA08008.1.
PIRiS64105.
RefSeqiNP_011417.1. NM_001180963.1.

Genome annotation databases

EnsemblFungiiYGL098W; YGL098W; YGL098W.
GeneIDi852780.
KEGGisce:YGL098W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72620 Genomic DNA. Translation: CAA96804.1.
AY557814 Genomic DNA. Translation: AAS56140.1.
BK006941 Genomic DNA. Translation: DAA08008.1.
PIRiS64105.
RefSeqiNP_011417.1. NM_001180963.1.

3D structure databases

ProteinModelPortaliP53146.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33151. 71 interactions.
DIPiDIP-5478N.
IntActiP53146. 9 interactions.
MINTiMINT-475168.

Proteomic databases

MaxQBiP53146.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL098W; YGL098W; YGL098W.
GeneIDi852780.
KEGGisce:YGL098W.

Organism-specific databases

EuPathDBiFungiDB:YGL098W.
SGDiS000003066. USE1.

Phylogenomic databases

HOGENOMiHOG000000925.
InParanoidiP53146.
KOiK08507.
OMAiDRQIEDQ.
OrthoDBiEOG092C5D1S.

Enzyme and pathway databases

BioCyciYEAST:G3O-30598-MONOMER.

Miscellaneous databases

PROiP53146.

Family and domain databases

InterProiIPR019150. Vesicle_transport_protein_Use1.
[Graphical view]
PfamiPF09753. Use1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUSE1_YEAST
AccessioniPrimary (citable) accession number: P53146
Secondary accession number(s): D6VU47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 937 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.