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Protein

Large subunit GTPase 1

Gene

LSG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GTPase required for the nuclear export of the 60S ribosomal subunit. Acts by mediating the release of NMD3 from the 60S ribosomal subunit after export into the cytoplasm.4 Publications

Miscellaneous

Present with 19400 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi236 – 239GTPSequence analysis4
Nucleotide bindingi343 – 350GTPCurated8
Nucleotide bindingi387 – 390GTPSequence analysis4

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • ascospore formation Source: SGD
  • conjugation with cellular fusion Source: SGD
  • ribosomal large subunit assembly Source: SGD
  • ribosomal subunit export from nucleus Source: SGD

Keywordsi

Molecular functionHydrolase
Biological processProtein transport, Transport
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30599-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Large subunit GTPase 1 (EC:3.6.1.-)
Gene namesi
Name:LSG1
Ordered Locus Names:YGL099W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL099W
SGDiS000003067 LSG1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi204I → T: Impairs the nuclear export of 60S ribosomal subunit in cytoplasm. 1 Publication1
Mutagenesisi349K → T: Dominant negative mutant; prevents the nuclear export of 60S ribosomal subunit in cytoplasm. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001224541 – 640Large subunit GTPase 1Add BLAST640

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei103PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53145
PaxDbiP53145
PRIDEiP53145

PTM databases

iPTMnetiP53145

Interactioni

Subunit structurei

Associates with the 60S ribosomal subunit. Interacts with ARB1.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi33150, 111 interactors
DIPiDIP-6659N
IntActiP53145, 36 interactors
MINTiP53145
STRINGi4932.YGL099W

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5T62electron microscopy3.30W1-640[»]
ProteinModelPortaliP53145
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini188 – 394CP-type GPROSITE-ProRule annotationAdd BLAST207

Domaini

In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.

Sequence similaritiesi

Belongs to the TRAFAC class YlqF/YawG GTPase family. LSG1 subfamily.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00530000063453
HOGENOMiHOG000172552
InParanoidiP53145
KOiK14539
OMAiYNRGFMT
OrthoDBiEOG092C35RK

Family and domain databases

InterProiView protein in InterPro
IPR030378 G_CP_dom
IPR006073 GTP_binding_domain
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF01926 MMR_HSR1, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS51721 G_CP, 1 hit

Sequencei

Sequence statusi: Complete.

P53145-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPKEAPKKW KAPKGPKPTH RKNKNKLELG RAIKYARQKE NAIEYLPDGE
60 70 80 90 100
MRFTTDKHEA NWVKLRSVTQ ESALDEFLST AALADKDFTA DRHSNVKIIR
110 120 130 140 150
MDSGNDSATS QGFSMTNEQR GNLNAKQRAL AKDLIVPRRP EWNEGMSKFQ
160 170 180 190 200
LDRQEKEAFL EWRRKLAHLQ ESNEDLLLTP FERNIEVWKQ LWRVVERSDL
210 220 230 240 250
VVQIVDARNP LLFRSVDLER YVKESDDRKA NLLLVNKADL LTKKQRIAWA
260 270 280 290 300
KYFISKNISF TFYSALRANQ LLEKQKEMGE DYREQDFEEA DKEGFDADEK
310 320 330 340 350
VMEKVKILSI DQLEELFLSK APNEPLLPPL PGQPPLINIG LVGYPNVGKS
360 370 380 390 400
STINSLVGAK KVSVSSTPGK TKHFQTIKLS DSVMLCDCPG LVFPNFAYNK
410 420 430 440 450
GELVCNGVLP IDQLRDYIGP AGLVAERIPK YYIEAIYGIH IQTKSRDEGG
460 470 480 490 500
NGDIPTAQEL LVAYARARGY MTQGYGSADE PRASRYILKD YVNGKLLYVN
510 520 530 540 550
PPPHLEDDTP YTREECEEFN KDLYVFDRLP DTRKEQVQNA AKAKGIDIVD
560 570 580 590 600
LARDLNQLTF SAHTGGDTQK EAKSVTHGGK QAALYNAAED LDRDFFKMNN
610 620 630 640
VEGRLSTPFH KVQNSSAGKR HNKKNKSKNA KSKVFSIENN
Length:640
Mass (Da):72,728
Last modified:October 1, 1996 - v1
Checksum:i15FD76541A2E8410
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72621 Genomic DNA Translation: CAA96805.1
BK006941 Genomic DNA Translation: DAA08007.1
PIRiS64106
RefSeqiNP_011416.3, NM_001180964.3

Genome annotation databases

EnsemblFungiiYGL099W; YGL099W; YGL099W
GeneIDi852779
KEGGisce:YGL099W

Similar proteinsi

Entry informationi

Entry nameiLSG1_YEAST
AccessioniPrimary (citable) accession number: P53145
Secondary accession number(s): D6VU46
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 158 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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