Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Large subunit GTPase 1

Gene

LSG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTPase required for the nuclear export of the 60S ribosomal subunit. Acts by mediating the release of NMD3 from the 60S ribosomal subunit after export into the cytoplasm.4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi236 – 2394GTPSequence analysis
Nucleotide bindingi343 – 3508GTPCurated
Nucleotide bindingi387 – 3904GTPSequence analysis

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • ascospore formation Source: SGD
  • conjugation with cellular fusion Source: SGD
  • protein transport Source: UniProtKB-KW
  • ribosomal large subunit assembly Source: SGD
  • ribosomal subunit export from nucleus Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30599-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Large subunit GTPase 1 (EC:3.6.1.-)
Gene namesi
Name:LSG1
Ordered Locus Names:YGL099W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL099W.
SGDiS000003067. LSG1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • cytosolic large ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi204 – 2041I → T: Impairs the nuclear export of 60S ribosomal subunit in cytoplasm. 1 Publication
Mutagenesisi349 – 3491K → T: Dominant negative mutant; prevents the nuclear export of 60S ribosomal subunit in cytoplasm. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 640640Large subunit GTPase 1PRO_0000122454Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei103 – 1031PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53145.
PeptideAtlasiP53145.

PTM databases

iPTMnetiP53145.

Interactioni

Subunit structurei

Associates with the 60S ribosomal subunit. Interacts with ARB1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
REI1P383447EBI-23885,EBI-21136
TIF6Q125225EBI-23885,EBI-9046
YVH1Q022563EBI-23885,EBI-14322

Protein-protein interaction databases

BioGridi33150. 70 interactions.
DIPiDIP-6659N.
IntActiP53145. 35 interactions.
MINTiMINT-614839.

Structurei

3D structure databases

ProteinModelPortaliP53145.
SMRiP53145. Positions 193-274, 337-393.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini188 – 394207CP-type GPROSITE-ProRule annotationAdd
BLAST

Domaini

In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.

Sequence similaritiesi

Belongs to the TRAFAC class YlqF/YawG GTPase family. LSG1 subfamily.PROSITE-ProRule annotation
Contains 1 CP-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00530000063453.
HOGENOMiHOG000172552.
InParanoidiP53145.
KOiK14539.
OMAiYTINHSS.
OrthoDBiEOG7SN8N7.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR030378. G_CP_dom.
IPR006073. GTP_binding_domain.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01926. MMR_HSR1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS51721. G_CP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53145-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPKEAPKKW KAPKGPKPTH RKNKNKLELG RAIKYARQKE NAIEYLPDGE
60 70 80 90 100
MRFTTDKHEA NWVKLRSVTQ ESALDEFLST AALADKDFTA DRHSNVKIIR
110 120 130 140 150
MDSGNDSATS QGFSMTNEQR GNLNAKQRAL AKDLIVPRRP EWNEGMSKFQ
160 170 180 190 200
LDRQEKEAFL EWRRKLAHLQ ESNEDLLLTP FERNIEVWKQ LWRVVERSDL
210 220 230 240 250
VVQIVDARNP LLFRSVDLER YVKESDDRKA NLLLVNKADL LTKKQRIAWA
260 270 280 290 300
KYFISKNISF TFYSALRANQ LLEKQKEMGE DYREQDFEEA DKEGFDADEK
310 320 330 340 350
VMEKVKILSI DQLEELFLSK APNEPLLPPL PGQPPLINIG LVGYPNVGKS
360 370 380 390 400
STINSLVGAK KVSVSSTPGK TKHFQTIKLS DSVMLCDCPG LVFPNFAYNK
410 420 430 440 450
GELVCNGVLP IDQLRDYIGP AGLVAERIPK YYIEAIYGIH IQTKSRDEGG
460 470 480 490 500
NGDIPTAQEL LVAYARARGY MTQGYGSADE PRASRYILKD YVNGKLLYVN
510 520 530 540 550
PPPHLEDDTP YTREECEEFN KDLYVFDRLP DTRKEQVQNA AKAKGIDIVD
560 570 580 590 600
LARDLNQLTF SAHTGGDTQK EAKSVTHGGK QAALYNAAED LDRDFFKMNN
610 620 630 640
VEGRLSTPFH KVQNSSAGKR HNKKNKSKNA KSKVFSIENN
Length:640
Mass (Da):72,728
Last modified:October 1, 1996 - v1
Checksum:i15FD76541A2E8410
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72621 Genomic DNA. Translation: CAA96805.1.
BK006941 Genomic DNA. Translation: DAA08007.1.
PIRiS64106.
RefSeqiNP_011416.3. NM_001180964.3.

Genome annotation databases

EnsemblFungiiYGL099W; YGL099W; YGL099W.
GeneIDi852779.
KEGGisce:YGL099W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72621 Genomic DNA. Translation: CAA96805.1.
BK006941 Genomic DNA. Translation: DAA08007.1.
PIRiS64106.
RefSeqiNP_011416.3. NM_001180964.3.

3D structure databases

ProteinModelPortaliP53145.
SMRiP53145. Positions 193-274, 337-393.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33150. 70 interactions.
DIPiDIP-6659N.
IntActiP53145. 35 interactions.
MINTiMINT-614839.

PTM databases

iPTMnetiP53145.

Proteomic databases

MaxQBiP53145.
PeptideAtlasiP53145.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL099W; YGL099W; YGL099W.
GeneIDi852779.
KEGGisce:YGL099W.

Organism-specific databases

EuPathDBiFungiDB:YGL099W.
SGDiS000003067. LSG1.

Phylogenomic databases

GeneTreeiENSGT00530000063453.
HOGENOMiHOG000172552.
InParanoidiP53145.
KOiK14539.
OMAiYTINHSS.
OrthoDBiEOG7SN8N7.

Enzyme and pathway databases

BioCyciYEAST:G3O-30599-MONOMER.

Miscellaneous databases

NextBioi972257.
PROiP53145.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR030378. G_CP_dom.
IPR006073. GTP_binding_domain.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01926. MMR_HSR1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS51721. G_CP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
    Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
    Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The putative GTPases Nog1p and Lsg1p are required for 60S ribosomal subunit biogenesis and are localized to the nucleus and cytoplasm, respectively."
    Kallstrom G., Hedges J., Johnson A.
    Mol. Cell. Biol. 23:4344-4355(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH 60S RIBOSOMAL SUBUNIT.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Release of the export adapter, Nmd3p, from the 60S ribosomal subunit requires Rpl10p and the cytoplasmic GTPase Lsg1p."
    Hedges J., West M., Johnson A.W.
    EMBO J. 24:567-579(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF ILE-204 AND LYS-349.
  8. "Defining the order in which Nmd3p and Rpl10p load onto nascent 60S ribosomal subunits."
    West M., Hedges J.B., Chen A., Johnson A.W.
    Mol. Cell. Biol. 25:3802-3813(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "The novel ATP-binding cassette protein ARB1 is a shuttling factor that stimulates 40S and 60S ribosome biogenesis."
    Dong J., Lai R., Jennings J.L., Link A.J., Hinnebusch A.G.
    Mol. Cell. Biol. 25:9859-9873(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ARB1.
  10. "Mapping the functional domains of yeast NMD3, the nuclear export adapter for the 60 S ribosomal subunit."
    Hedges J., Chen Y.I., West M., Bussiere C., Johnson A.W.
    J. Biol. Chem. 281:36579-36587(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Mutational analysis of the ribosomal protein Rpl10 from yeast."
    Hofer A., Bussiere C., Johnson A.W.
    J. Biol. Chem. 282:32630-32639(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-103, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiLSG1_YEAST
AccessioniPrimary (citable) accession number: P53145
Secondary accession number(s): D6VU46
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 11, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 19400 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.