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P53142 (VPS73_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vacuolar protein sorting-associated protein 73
Gene names
Name:VPS73
Ordered Locus Names:YGL104C
ORF Names:G3090
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length486 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in vacuolar protein sorting. Ref.5

Subcellular location

Mitochondrion membrane; Multi-pass membrane protein Ref.6.

Sequence similarities

Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 486486Vacuolar protein sorting-associated protein 73
PRO_0000050462

Regions

Topological domain1 – 2626Cytoplasmic Potential
Transmembrane27 – 4721Helical; Name=1; Potential
Topological domain48 – 9043Mitochondrial intermembrane Potential
Transmembrane91 – 11121Helical; Name=2; Potential
Topological domain112 – 1198Cytoplasmic Potential
Transmembrane120 – 14021Helical; Name=3; Potential
Topological domain141 – 1466Mitochondrial intermembrane Potential
Transmembrane147 – 16721Helical; Name=4; Potential
Topological domain168 – 17811Cytoplasmic Potential
Transmembrane179 – 19921Helical; Name=5; Potential
Topological domain200 – 2089Mitochondrial intermembrane Potential
Transmembrane209 – 22921Helical; Name=6; Potential
Topological domain230 – 30576Cytoplasmic Potential
Transmembrane306 – 32621Helical; Name=7; Potential
Topological domain327 – 34216Mitochondrial intermembrane Potential
Transmembrane343 – 36321Helical; Name=8; Potential
Topological domain364 – 3663Cytoplasmic Potential
Transmembrane367 – 38721Helical; Name=9; Potential
Topological domain388 – 3969Mitochondrial intermembrane Potential
Transmembrane397 – 41721Helical; Name=10; Potential
Topological domain418 – 43215Cytoplasmic Potential
Transmembrane433 – 45321Helical; Name=11; Potential
Topological domain4541Mitochondrial intermembrane Potential
Transmembrane455 – 47521Helical; Name=12; Potential
Topological domain476 – 48611Cytoplasmic Potential

Amino acid modifications

Modified residue61Phosphoserine Ref.8

Sequences

Sequence LengthMass (Da)Tools
P53142 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: D924D9112516450D

FASTA48654,331
        10         20         30         40         50         60 
MNRILSSASL LSNVSMPRQN KHKITKALCY AIIVASIGSI QFGYHLSELN APQQVLSCSE 

        70         80         90        100        110        120 
FDIPMEGYPY DRTWLGKRGY KQCIPLNDEQ IGIVTSVFCI GGILGSYFAT SLANIYGRKF 

       130        140        150        160        170        180 
SSLINCTLNI VGSLIIFNSN SYRGLIIGRI LVGISCGSLI VIIPLFIKEV APSGWEGLLG 

       190        200        210        220        230        240 
SMTQICIRLG VLLTQGIALP LTDSYRWRWI LFGSFLIAVL NFFMWFIVDE SPKWLLAHGR 

       250        260        270        280        290        300 
VTDAKLSLCK LRGVTFDEAA QEIQDWQLQI ESGDPLIEPT TTNSISGSNS LWKYLRDRTN 

       310        320        330        340        350        360 
VKSRHVITVL LFGQQFCGIN SIVLYGTKII SQLYPQHAIR INFFISMVNV LVTILVSLLI 

       370        380        390        400        410        420 
HSLPRKPLLM TSTVLVSVTA FIMGIAMNHN KMNLLIVFSF IYMGVFTMGL NPLPFIIMRE 

       430        440        450        460        470        480 
VSKPQDMVLA QRYGTICNWV GTFIIAYTFP IIHDVLSGYV FIIFAIIACS ISAFIWKKVP 


ETKRSG

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-151.
Strain: ATCC 204508 / S288c.
[4]"The genes encoding the transcription factor yTAFII60, the G4p1 protein and a putative glucose transporter are contained in a 12.3 kb DNA fragment on the left arm of Saccharomyces cerevisiae chromosome VII."
Paoluzi S., Minenkova O., Castagnoli L.
Yeast 13:85-91(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 150-486.
[5]"Genomic screen for vacuolar protein sorting genes in Saccharomyces cerevisiae."
Bonangelino C.J., Chavez E.M., Bonifacino J.S.
Mol. Biol. Cell 13:2486-2501(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"The proteome of Saccharomyces cerevisiae mitochondria."
Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C.
Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
Strain: ATCC 76625 / YPH499.
[7]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-6, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Z72626 Genomic DNA. Translation: CAA96811.1.
Z72625 Genomic DNA. Translation: CAA96810.1.
X97644 Genomic DNA. Translation: CAA66249.1.
BK006941 Genomic DNA. Translation: DAA08003.1.
PIRS64111.
RefSeqNP_011411.3. NM_001180969.3.

3D structure databases

ProteinModelPortalP53142.
SMRP53142. Positions 91-483.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-1727N.
IntActP53142. 8 interactions.
MINTMINT-401590.
STRING4932.YGL104C.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGL104C; YGL104C; YGL104C.
GeneID852774.
KEGGsce:YGL099W.
sce:YGL104C.

Organism-specific databases

CYGDYGL104c.
SGDS000003072. VPS73.

Phylogenomic databases

eggNOGCOG0477.
GeneTreeENSGT00630000089621.
HOGENOMHOG000199277.
KOK14539.
OMAHAIRINF.
OrthoDBEOG408RHR.

Gene expression databases

GenevestigatorP53142.
GermOnlineYGL104C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR005828. Sub_transporter.
IPR003663. Sugar/inositol_transpt.
[Graphical view]
PfamPF00083. Sugar_tr. 1 hit.
[Graphical view]
PRINTSPR00171. SUGRTRNSPORT.
SUPFAMSSF103473. MFS_gen_substrate_transporter. 1 hit.
PROSITEPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. False negative.
PS00217. SUGAR_TRANSPORT_2. False negative.
[Graphical view]
ProtoNetSearch...

Other

NextBio972245.

Entry information

Entry nameVPS73_YEAST
AccessionPrimary (citable) accession number: P53142
Secondary accession number(s): D6VU42
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 1, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents