Myosin light chain 1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

P53141 (MLC1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 115. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Myosin light chain 1

Alternative name(s):
Calmodulin-like myosin light chain MLC1
Myosin-2 light chain

Gene names

Name:	MLC1
Ordered Locus Names:	YGL106W
ORF Names:	G3080

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	149 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential light chain for the class II conventional myosin MYO1. Acts also as light chain for the class V unconventional myosin MYO2 and for IQG1. Involved in the assembly of the contractile actomyosin ring at the bud neck during cytokinesis by recruiting IQG1 to the bud neck. Also required for chitin and MYO2-dependent secretory vesicle deposition to the center of the bud neck for septum formation. May stabilize MYO2 by binding to its IQ domains. Its major function is probably not to regulate MYO1 activity, but rather to coordinate actin ring formation and targeted membrane deposition during cytokinesis via its interactions with MYO1, IQG1 and MYO2. Ref.4 Ref.7 Ref.8
Subunit structure	Interacts with MYO1, MYO2 and IQG1 by binding to their IQ domains. Interacts with SEC4. Ref.4 Ref.5 Ref.6 Ref.7 Ref.8
Subcellular location	Bud neck. Bud tip. Note: Concentrates to sites of polarized growth, namely to the incipient bud site in G1, to the bud tip during S and G2 phases of the cell cycle and to the bud neck during cytokinesis. Ref.5 Ref.6
Sequence similarities	Contains 3 EF-hand domains.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Domain	Repeat
Ligand	Calcium
Molecular function	Motor protein Myosin
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cytokinesis, actomyosin contractile ring assembly Inferred from mutant phenotype Ref.8. Source: SGD protein localization Inferred from mutant phenotype Ref.6. Source: SGD vesicle targeting Inferred from mutant phenotype Ref.7. Source: SGD
Cellular component	cellular bud neck Inferred from direct assay Ref.8. Source: SGD cellular bud tip Inferred from direct assay Ref.8. Source: SGD myosin complex Inferred from electronic annotation. Source: UniProtKB-KW vesicle Inferred from direct assay Ref.7. Source: SGD
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro myosin II heavy chain binding Inferred from physical interaction Ref.8. Source: SGD myosin V binding Inferred from direct assay Ref.4. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
IQG1	Q12280	7	EBI-10988,EBI-35351
MYO1	P08964	3	EBI-10988,EBI-11650

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 149

149

Myosin light chain 1

PRO_0000198765

Regions

Domain

2 – 37

EF-hand 1

Domain

81 – 116

EF-hand 2

Domain

117 – 149

EF-hand 3

Calcium binding

15 – 26

1 Potential

Calcium binding

94 – 105

2 Potential

Calcium binding

121 – 132

3 Potential

Experimental info

Mutagenesis

F → A in MLC1-1; causes a defect in septum formation. Ref.7

Mutagenesis

114

G → D in MLC1-11; abolishes interaction with MYO2 and IQG1 and reduces interaction with MYO1. Leads to mislocalization of IQG1 and a severe defect in cytokinesis. Ref.8

Mutagenesis

135

G → E in MLC1-93; reduces interaction with MYO1, but does not cause any defect in cytokinesis. Ref.8

Mutagenesis

142

F → A in MLC1-5; causes a defect in septum formation. Ref.7

Secondary structure

149

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P53141 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: D7508123C101E4A6
Show »

FASTA

149

16,444

        10         20         30         40         50         60 
MSATRANKDI FTLFDKKGQG AIAKDSLGDY LRAIGYNPTN QLVQDIINAD SSLRDASSLT 

        70         80         90        100        110        120 
LDQITGLIEV NEKELDATTK AKTEDFVKAF QVFDKESTGK VSVGDLRYML TGLGEKLTDA 

       130        140 
EVDELLKGVE VDSNGEIDYK KFIEDVLRQ

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genes encoding the transcription factor yTAFII60, the G4p1 protein and a putative glucose transporter are contained in a 12.3 kb DNA fragment on the left arm of Saccharomyces cerevisiae chromosome VII." Paoluzi S., Minenkova O., Castagnoli L. Yeast 13:85-91(1997) [PubMed: 9046090] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Mlc1p is a light chain for the unconventional myosin Myo2p in Saccharomyces cerevisiae." Stevens R.C., Davis T.N. J. Cell Biol. 142:711-722(1998) [PubMed: 9700160] [Abstract] Cited for: FUNCTION, INTERACTION WITH MYO2.
[5]	"A myosin light chain mediates the localization of the budding yeast IQGAP-like protein during contractile ring formation." Shannon K.B., Li R. Curr. Biol. 10:727-730(2000) [PubMed: 10873803] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH IQG1.
[6]	"Yeast myosin light chain, Mlc1p, interacts with both IQGAP and class II myosin to effect cytokinesis." Boyne J.R., Yosuf H.M., Bieganowski P., Brenner C., Price C. J. Cell Sci. 113:4533-4543(2000) [PubMed: 11082046] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH IQG1 AND MYO1.
[7]	"Mlc1p promotes septum closure during cytokinesis via the IQ motifs of the vesicle motor Myo2p." Wagner W., Bielli P., Wacha S., Ragnini-Wilson A. EMBO J. 21:6397-6408(2002) [PubMed: 12456647] [Abstract] Cited for: FUNCTION, INTERACTION WITH MYO2 AND SEC4, MUTAGENESIS OF PHE-93 AND PHE-142.
[8]	"Identification and functional analysis of the essential and regulatory light chains of the only type II myosin Myo1p in Saccharomyces cerevisiae." Luo J., Vallen E.A., Dravis C., Tcheperegine S.E., Drees B., Bi E. J. Cell Biol. 165:843-855(2004) [PubMed: 15210731] [Abstract] Cited for: FUNCTION, INTERACTION WITH MYO1, MUTAGENESIS OF GLY-114 AND GLY-135.
[9]	"Crystallization, X-ray characterization and selenomethionine phasing of Mlc1p bound to IQ motifs from myosin V." Terrak M., Otterbein L.R., Wu G., Palecanda L.A., Lu R.C., Dominguez R. Acta Crystallogr. D 58:1882-1885(2002) [PubMed: 12351846] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 2-149 IN COMPLEX WITH MYO2.
[10]	"Two distinct myosin light chain structures are induced by specific variations within the bound IQ motifs-functional implications." Terrak M., Wu G., Stafford W.F., Lu R.C., Dominguez R. EMBO J. 22:362-371(2003) [PubMed: 12554638] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 2-149 IN COMPLEX WITH MYO2.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X97644 Genomic DNA. Translation: CAA66246.1.
Z72628 Genomic DNA. Translation: CAA96813.1.
BK006941 Genomic DNA. Translation: DAA08001.1.

PIR

S64114.

RefSeq

NP_011409.1. NM_001180971.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1M45	X-ray	1.65	A	2-149	[»]
1M46	X-ray	2.10	A	2-149	[»]
1N2D	X-ray	2.00	A/B	2-149	[»]
2FCD	NMR	-	A	2-79	[»]
2FCE	NMR	-	A	80-149	[»]

ProteinModelPortal

P53141.

SMR

P53141. Positions 3-149.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-5576N.

IntAct

P53141. 18 interactions.

MINT

MINT-562145.

STRING

P53141.

Proteomic databases

PeptideAtlas

P53141.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YGL106W; YGL106W; YGL106W.

GeneID

852772.

KEGG

sce:YGL106W.

NMPDR

fig|4932.3.peg.2514.

Organism-specific databases

CYGD

YGL106w.

SGD

S000003074. MLC1.

Phylogenomic databases

eggNOG

fuNOG09931.

GeneTree

EFGT00050000003660.

HOGENOM

HBG746798.

OMA

IDYKKFI.

OrthoDB

EOG4W6S5M.

Gene expression databases

ArrayExpress

P53141.

Genevestigator

P53141.

GermOnline

YGL106W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR011992. EF-hand-like_dom.
IPR018249. EF_HAND_2.
IPR002048. EF_hand_Ca-bd.
[Graphical view]

Gene3D

G3DSA:1.10.238.10. EF-Hand_type. 2 hits.

SMART

SM00054. EFh. 3 hits.
[Graphical view]

PROSITE

PS00018. EF_HAND_1. False negative.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

ProtoNet

Search...

Other

NextBio

972239.

Entry information

Entry name

MLC1_YEAST

Accession

Primary (citable) accession number: P53141
Secondary accession number(s): D6VU40

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 115 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents