Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Myosin light chain 1

Gene

MLC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential light chain for the class II conventional myosin MYO1. Acts also as light chain for the class V unconventional myosin MYO2 and for IQG1. Involved in the assembly of the contractile actomyosin ring at the bud neck during cytokinesis by recruiting IQG1 to the bud neck. Also required for chitin and MYO2-dependent secretory vesicle deposition to the center of the bud neck for septum formation. May stabilize MYO2 by binding to its IQ domains. Its major function is probably not to regulate MYO1 activity, but rather to coordinate actin ring formation and targeted membrane deposition during cytokinesis via its interactions with MYO1, IQG1 and MYO2.3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi15 – 261PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi94 – 1052PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi121 – 1323PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • myosin II heavy chain binding Source: SGD
  • myosin V binding Source: SGD

GO - Biological processi

  • actomyosin contractile ring assembly Source: SGD
  • protein localization Source: SGD
  • vesicle targeting Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Myosin

Keywords - Biological processi

Cell cycle, Cell division

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30605-MONOMER.
ReactomeiR-SCE-445355. Smooth Muscle Contraction.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin light chain 1
Alternative name(s):
Calmodulin-like myosin light chain MLC1
Myosin-2 light chain
Gene namesi
Name:MLC1
Ordered Locus Names:YGL106W
ORF Names:G3080
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL106W.
SGDiS000003074. MLC1.

Subcellular locationi

  • Bud neck 2 Publications
  • Bud tip 2 Publications

  • Note: Concentrates to sites of polarized growth, namely to the incipient bud site in G1, to the bud tip during S and G2 phases of the cell cycle and to the bud neck during cytokinesis.1 Publication

GO - Cellular componenti

  • cellular bud neck Source: SGD
  • cellular bud tip Source: SGD
  • myosin II complex Source: SGD
  • vesicle Source: SGD
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi93F → A in MLC1-1; causes a defect in septum formation. 1 Publication1
Mutagenesisi114G → D in MLC1-11; abolishes interaction with MYO2 and IQG1 and reduces interaction with MYO1. Leads to mislocalization of IQG1 and a severe defect in cytokinesis. 1 Publication1
Mutagenesisi135G → E in MLC1-93; reduces interaction with MYO1, but does not cause any defect in cytokinesis. 1 Publication1
Mutagenesisi142F → A in MLC1-5; causes a defect in septum formation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001987651 – 149Myosin light chain 1Add BLAST149

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki116Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP53141.
PRIDEiP53141.
TopDownProteomicsiP53141.

Interactioni

Subunit structurei

Interacts with MYO1, MYO2 and IQG1 by binding to their IQ domains. Interacts with SEC4.7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-10988,EBI-10988
IQG1Q122807EBI-10988,EBI-35351
MYO1P089643EBI-10988,EBI-11650

GO - Molecular functioni

  • myosin II heavy chain binding Source: SGD
  • myosin V binding Source: SGD

Protein-protein interaction databases

BioGridi33144. 83 interactors.
DIPiDIP-5576N.
IntActiP53141. 19 interactors.
MINTiMINT-562145.

Structurei

Secondary structure

1149
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi11 – 14Combined sources4
Beta strandi20 – 23Combined sources4
Helixi24 – 26Combined sources3
Helixi27 – 33Combined sources7
Helixi40 – 48Combined sources9
Helixi51 – 55Combined sources5
Beta strandi58 – 60Combined sources3
Helixi61 – 70Combined sources10
Helixi72 – 76Combined sources5
Helixi77 – 79Combined sources3
Helixi84 – 91Combined sources8
Beta strandi95 – 102Combined sources8
Helixi103 – 112Combined sources10
Beta strandi113 – 115Combined sources3
Helixi119 – 126Combined sources8
Beta strandi135 – 138Combined sources4
Helixi139 – 147Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1M45X-ray1.65A2-149[»]
1M46X-ray2.10A2-149[»]
1N2DX-ray2.00A/B2-149[»]
2FCDNMR-A2-79[»]
2FCENMR-A80-149[»]
ProteinModelPortaliP53141.
SMRiP53141.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53141.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 37EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini81 – 116EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini117 – 149EF-hand 3PROSITE-ProRule annotationAdd BLAST33

Sequence similaritiesi

Contains 3 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00590000082921.
HOGENOMiHOG000233018.
InParanoidiP53141.
OMAiQDGQINY.
OrthoDBiEOG092C4XFN.

Family and domain databases

CDDicd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS50222. EF_HAND_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53141-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSATRANKDI FTLFDKKGQG AIAKDSLGDY LRAIGYNPTN QLVQDIINAD
60 70 80 90 100
SSLRDASSLT LDQITGLIEV NEKELDATTK AKTEDFVKAF QVFDKESTGK
110 120 130 140
VSVGDLRYML TGLGEKLTDA EVDELLKGVE VDSNGEIDYK KFIEDVLRQ
Length:149
Mass (Da):16,444
Last modified:October 1, 1996 - v1
Checksum:iD7508123C101E4A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97644 Genomic DNA. Translation: CAA66246.1.
Z72628 Genomic DNA. Translation: CAA96813.1.
BK006941 Genomic DNA. Translation: DAA08001.1.
PIRiS64114.
RefSeqiNP_011409.1. NM_001180971.1.

Genome annotation databases

EnsemblFungiiYGL106W; YGL106W; YGL106W.
GeneIDi852772.
KEGGisce:YGL106W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97644 Genomic DNA. Translation: CAA66246.1.
Z72628 Genomic DNA. Translation: CAA96813.1.
BK006941 Genomic DNA. Translation: DAA08001.1.
PIRiS64114.
RefSeqiNP_011409.1. NM_001180971.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1M45X-ray1.65A2-149[»]
1M46X-ray2.10A2-149[»]
1N2DX-ray2.00A/B2-149[»]
2FCDNMR-A2-79[»]
2FCENMR-A80-149[»]
ProteinModelPortaliP53141.
SMRiP53141.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33144. 83 interactors.
DIPiDIP-5576N.
IntActiP53141. 19 interactors.
MINTiMINT-562145.

Proteomic databases

MaxQBiP53141.
PRIDEiP53141.
TopDownProteomicsiP53141.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL106W; YGL106W; YGL106W.
GeneIDi852772.
KEGGisce:YGL106W.

Organism-specific databases

EuPathDBiFungiDB:YGL106W.
SGDiS000003074. MLC1.

Phylogenomic databases

GeneTreeiENSGT00590000082921.
HOGENOMiHOG000233018.
InParanoidiP53141.
OMAiQDGQINY.
OrthoDBiEOG092C4XFN.

Enzyme and pathway databases

BioCyciYEAST:G3O-30605-MONOMER.
ReactomeiR-SCE-445355. Smooth Muscle Contraction.

Miscellaneous databases

EvolutionaryTraceiP53141.
PROiP53141.

Family and domain databases

CDDicd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMLC1_YEAST
AccessioniPrimary (citable) accession number: P53141
Secondary accession number(s): D6VU40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 157 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.