Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Myosin light chain 1

Gene

MLC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential light chain for the class II conventional myosin MYO1. Acts also as light chain for the class V unconventional myosin MYO2 and for IQG1. Involved in the assembly of the contractile actomyosin ring at the bud neck during cytokinesis by recruiting IQG1 to the bud neck. Also required for chitin and MYO2-dependent secretory vesicle deposition to the center of the bud neck for septum formation. May stabilize MYO2 by binding to its IQ domains. Its major function is probably not to regulate MYO1 activity, but rather to coordinate actin ring formation and targeted membrane deposition during cytokinesis via its interactions with MYO1, IQG1 and MYO2.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi15 – 26121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi94 – 105122PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi121 – 132123PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • identical protein binding Source: IntAct
  • myosin II heavy chain binding Source: SGD
  • myosin V binding Source: SGD

GO - Biological processi

  • actomyosin contractile ring assembly Source: SGD
  • protein localization Source: SGD
  • vesicle targeting Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Myosin

Keywords - Biological processi

Cell cycle, Cell division

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30605-MONOMER.
ReactomeiR-SCE-445355. Smooth Muscle Contraction.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin light chain 1
Alternative name(s):
Calmodulin-like myosin light chain MLC1
Myosin-2 light chain
Gene namesi
Name:MLC1
Ordered Locus Names:YGL106W
ORF Names:G3080
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL106W.
SGDiS000003074. MLC1.

Subcellular locationi

  • Bud neck 2 Publications
  • Bud tip 2 Publications

  • Note: Concentrates to sites of polarized growth, namely to the incipient bud site in G1, to the bud tip during S and G2 phases of the cell cycle and to the bud neck during cytokinesis.1 Publication

GO - Cellular componenti

  • cellular bud neck Source: SGD
  • cellular bud tip Source: SGD
  • myosin II complex Source: SGD
  • vesicle Source: SGD
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi93 – 931F → A in MLC1-1; causes a defect in septum formation. 1 Publication
Mutagenesisi114 – 1141G → D in MLC1-11; abolishes interaction with MYO2 and IQG1 and reduces interaction with MYO1. Leads to mislocalization of IQG1 and a severe defect in cytokinesis. 1 Publication
Mutagenesisi135 – 1351G → E in MLC1-93; reduces interaction with MYO1, but does not cause any defect in cytokinesis. 1 Publication
Mutagenesisi142 – 1421F → A in MLC1-5; causes a defect in septum formation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 149149Myosin light chain 1PRO_0000198765Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki116 – 116Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP53141.
TopDownProteomicsiP53141.

Interactioni

Subunit structurei

Interacts with MYO1, MYO2 and IQG1 by binding to their IQ domains. Interacts with SEC4.7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-10988,EBI-10988
IQG1Q122807EBI-10988,EBI-35351
MYO1P089643EBI-10988,EBI-11650

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • myosin II heavy chain binding Source: SGD
  • myosin V binding Source: SGD

Protein-protein interaction databases

BioGridi33144. 82 interactions.
DIPiDIP-5576N.
IntActiP53141. 19 interactions.
MINTiMINT-562145.

Structurei

Secondary structure

1
149
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi11 – 144Combined sources
Beta strandi20 – 234Combined sources
Helixi24 – 263Combined sources
Helixi27 – 337Combined sources
Helixi40 – 489Combined sources
Helixi51 – 555Combined sources
Beta strandi58 – 603Combined sources
Helixi61 – 7010Combined sources
Helixi72 – 765Combined sources
Helixi77 – 793Combined sources
Helixi84 – 918Combined sources
Beta strandi95 – 1028Combined sources
Helixi103 – 11210Combined sources
Beta strandi113 – 1153Combined sources
Helixi119 – 1268Combined sources
Beta strandi135 – 1384Combined sources
Helixi139 – 1479Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1M45X-ray1.65A2-149[»]
1M46X-ray2.10A2-149[»]
1N2DX-ray2.00A/B2-149[»]
2FCDNMR-A2-79[»]
2FCENMR-A80-149[»]
ProteinModelPortaliP53141.
SMRiP53141. Positions 3-149.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53141.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 3736EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini81 – 11636EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini117 – 14933EF-hand 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 3 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00590000082921.
HOGENOMiHOG000233018.
InParanoidiP53141.
OMAiQDGQINY.
OrthoDBiEOG092C4XFN.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS50222. EF_HAND_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53141-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSATRANKDI FTLFDKKGQG AIAKDSLGDY LRAIGYNPTN QLVQDIINAD
60 70 80 90 100
SSLRDASSLT LDQITGLIEV NEKELDATTK AKTEDFVKAF QVFDKESTGK
110 120 130 140
VSVGDLRYML TGLGEKLTDA EVDELLKGVE VDSNGEIDYK KFIEDVLRQ
Length:149
Mass (Da):16,444
Last modified:October 1, 1996 - v1
Checksum:iD7508123C101E4A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97644 Genomic DNA. Translation: CAA66246.1.
Z72628 Genomic DNA. Translation: CAA96813.1.
BK006941 Genomic DNA. Translation: DAA08001.1.
PIRiS64114.
RefSeqiNP_011409.1. NM_001180971.1.

Genome annotation databases

EnsemblFungiiYGL106W; YGL106W; YGL106W.
GeneIDi852772.
KEGGisce:YGL106W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97644 Genomic DNA. Translation: CAA66246.1.
Z72628 Genomic DNA. Translation: CAA96813.1.
BK006941 Genomic DNA. Translation: DAA08001.1.
PIRiS64114.
RefSeqiNP_011409.1. NM_001180971.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1M45X-ray1.65A2-149[»]
1M46X-ray2.10A2-149[»]
1N2DX-ray2.00A/B2-149[»]
2FCDNMR-A2-79[»]
2FCENMR-A80-149[»]
ProteinModelPortaliP53141.
SMRiP53141. Positions 3-149.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33144. 82 interactions.
DIPiDIP-5576N.
IntActiP53141. 19 interactions.
MINTiMINT-562145.

Proteomic databases

MaxQBiP53141.
TopDownProteomicsiP53141.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL106W; YGL106W; YGL106W.
GeneIDi852772.
KEGGisce:YGL106W.

Organism-specific databases

EuPathDBiFungiDB:YGL106W.
SGDiS000003074. MLC1.

Phylogenomic databases

GeneTreeiENSGT00590000082921.
HOGENOMiHOG000233018.
InParanoidiP53141.
OMAiQDGQINY.
OrthoDBiEOG092C4XFN.

Enzyme and pathway databases

BioCyciYEAST:G3O-30605-MONOMER.
ReactomeiR-SCE-445355. Smooth Muscle Contraction.

Miscellaneous databases

EvolutionaryTraceiP53141.
PROiP53141.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMLC1_YEAST
AccessioniPrimary (citable) accession number: P53141
Secondary accession number(s): D6VU40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 154 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.