ID   YGK8_YEAST              Reviewed;         140 AA.
AC   P53139; D6VU38;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   27-MAR-2024, entry version 130.
DE   RecName: Full=Uncharacterized protein YGL108C;
GN   OrderedLocusNames=YGL108C; ORFNames=G3070;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9046090;
RX   DOI=10.1002/(sici)1097-0061(199701)13:1<85::aid-yea53>3.0.co;2-e;
RA   Paoluzi S., Minenkova O., Castagnoli L.;
RT   "The genes encoding the transcription factor yTAFII60, the G4p1 protein and
RT   a putative glucose transporter are contained in a 12.3 kb DNA fragment on
RT   the left arm of Saccharomyces cerevisiae chromosome VII.";
RL   Yeast 13:85-91(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169869;
RA   Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA   Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA   Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA   Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA   Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA   Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA   Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA   Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA   Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA   Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA   Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA   Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA   Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA   Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA   Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA   Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA   Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA   Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA   Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA   Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA   Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA   Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL   Nature 387:81-84(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [5]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [6]
RP   PALMITOYLATION.
RX   PubMed=16751107; DOI=10.1016/j.cell.2006.03.042;
RA   Roth A.F., Wan J., Bailey A.O., Sun B., Kuchar J.A., Green W.N.,
RA   Phinney B.S., Yates J.R. III, Davis N.G.;
RT   "Global analysis of protein palmitoylation in yeast.";
RL   Cell 125:1003-1013(2006).
RN   [7]
RP   MYRISTOYLATION AT GLY-2, AND PALMITOYLATION AT CYS-4 BY ERF2; PFA4 AND
RP   PFA5.
RX   PubMed=19416974; DOI=10.1074/jbc.m109.005447;
RA   Nadolski M.J., Linder M.E.;
RT   "Molecular recognition of the palmitoylation substrate Vac8 by its
RT   palmitoyltransferase Pfa3.";
RL   J. Biol. Chem. 284:17720-17730(2009).
RN   [8]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=26928762; DOI=10.1038/nmeth.3795;
RA   Yofe I., Weill U., Meurer M., Chuartzman S., Zalckvar E., Goldman O.,
RA   Ben-Dor S., Schuetze C., Wiedemann N., Knop M., Khmelinskii A.,
RA   Schuldiner M.;
RT   "One library to make them all: streamlining the creation of yeast libraries
RT   via a SWAp-Tag strategy.";
RL   Nat. Methods 13:371-378(2016).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:26928762}.
CC       Note=The protein localizes to the cytosol when tagged with a
CC       fluorescent protein at the C-terminus, and to the cell periphery when
CC       tagged with a fluorescent protein at the C-terminus.
CC       {ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:26928762}.
CC   -!- PTM: Myristoylated. {ECO:0000269|PubMed:19416974}.
CC   -!- PTM: The N-myristoylated protein is further palmitoylated by ERF2, PFA4
CC       and slightly by PFA5, but not by PFA3. {ECO:0000269|PubMed:16751107,
CC       ECO:0000269|PubMed:19416974}.
CC   -!- MISCELLANEOUS: Present with 2430 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: To S.pombe new13. {ECO:0000305}.
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DR   EMBL; X97644; CAA66244.1; -; Genomic_DNA.
DR   EMBL; Z72631; CAA96816.1; -; Genomic_DNA.
DR   EMBL; BK006941; DAA07999.1; -; Genomic_DNA.
DR   PIR; S64116; S64116.
DR   RefSeq; NP_011407.3; NM_001180973.3.
DR   AlphaFoldDB; P53139; -.
DR   SMR; P53139; -.
DR   BioGRID; 33142; 35.
DR   IntAct; P53139; 12.
DR   MINT; P53139; -.
DR   STRING; 4932.YGL108C; -.
DR   iPTMnet; P53139; -.
DR   SwissPalm; P53139; -.
DR   MaxQB; P53139; -.
DR   PaxDb; 4932-YGL108C; -.
DR   PeptideAtlas; P53139; -.
DR   EnsemblFungi; YGL108C_mRNA; YGL108C; YGL108C.
DR   GeneID; 852770; -.
DR   KEGG; sce:YGL108C; -.
DR   AGR; SGD:S000003076; -.
DR   SGD; S000003076; YGL108C.
DR   VEuPathDB; FungiDB:YGL108C; -.
DR   eggNOG; ENOG502S7W1; Eukaryota.
DR   HOGENOM; CLU_154049_0_0_1; -.
DR   InParanoid; P53139; -.
DR   OMA; HTEMPAK; -.
DR   OrthoDB; 2034696at2759; -.
DR   BioCyc; YEAST:G3O-30607-MONOMER; -.
DR   BioGRID-ORCS; 852770; 4 hits in 10 CRISPR screens.
DR   PRO; PR:P53139; -.
DR   Proteomes; UP000002311; Chromosome VII.
DR   RNAct; P53139; Protein.
DR   GO; GO:0005829; C:cytosol; HDA:SGD.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
PE   1: Evidence at protein level;
KW   Cytoplasm; Lipoprotein; Myristate; Palmitate; Reference proteome.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000255"
FT   CHAIN           2..140
FT                   /note="Uncharacterized protein YGL108C"
FT                   /id="PRO_0000202751"
FT   REGION          1..140
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        7..24
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        66..140
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   LIPID           2
FT                   /note="N-myristoyl glycine"
FT                   /evidence="ECO:0000255, ECO:0000269|PubMed:19416974"
FT   LIPID           4
FT                   /note="S-palmitoyl cysteine"
FT                   /evidence="ECO:0000305|PubMed:19416974"
SQ   SEQUENCE   140 AA;  16037 MW;  89C6AEC296CA9E57 CRC64;
     MGLCGSKTQP MPSQTTTVAT KARTKPINRD TVKSKQELRH KEKKDKKKKT QLKSTTVPVV
     QRKEGSKLTD TSDPSKNKVS PKEAARLAAE KRFQETNEKY NKGELGKKLA QERAKSHKTR
     LMEEAEKKHA ERERENMIYD
//