P53139 (YGK8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized protein YGL108C

Gene names

Ordered Locus Names:	YGL108C
ORF Names:	G3070

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	140 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Cell membrane; Lipid-anchor Ref.4.
Post-translational modification	Myristoylated. The N-myristoylated protein is further palmitoylated by ERF2, PFA4 and slightly by PFA5, but not by PFA3. Ref.6 Ref.7
Miscellaneous	Present with 2430 molecules/cell in log phase SD medium.
Sequence similarities	To S.pombe new13.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
PTM	Lipoprotein Myristate Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Potential
	Chain	2 – 140	139	Uncharacterized protein YGL108C		PRO_0000202751
Regions
	Compositional bias	41 – 49	9	Poly-Lys
Amino acid modifications
	Lipidation	2	1	N-myristoyl glycine Potential
	Lipidation	4	1	S-palmitoyl cysteine Probable

Sequences

	Sequence		Length	Mass (Da)
	P53139 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 89C6AEC296CA9E57	FASTA	140	16,037
	10 20 30 40 50 60 MGLCGSKTQP MPSQTTTVAT KARTKPINRD TVKSKQELRH KEKKDKKKKT QLKSTTVPVV 70 80 90 100 110 120 QRKEGSKLTD TSDPSKNKVS PKEAARLAAE KRFQETNEKY NKGELGKKLA QERAKSHKTR 130 140 LMEEAEKKHA ERERENMIYD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genes encoding the transcription factor yTAFII60, the G4p1 protein and a putative glucose transporter are contained in a 12.3 kb DNA fragment on the left arm of Saccharomyces cerevisiae chromosome VII." Paoluzi S., Minenkova O., Castagnoli L. Yeast 13:85-91(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein palmitoylation in yeast." Roth A.F., Wan J., Bailey A.O., Sun B., Kuchar J.A., Green W.N., Phinney B.S., Yates J.R. III, Davis N.G. Cell 125:1003-1013(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PALMITOYLATION.
[7]	"Molecular recognition of the palmitoylation substrate Vac8 by its palmitoyltransferase Pfa3." Nadolski M.J., Linder M.E. J. Biol. Chem. 284:17720-17730(2009) [PubMed] [Europe PMC] [Abstract] Cited for: MYRISTOYLATION, PALMITOYLATION BY ERF2; PFA4 AND PFA5.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X97644 Genomic DNA. Translation: CAA66244.1. Z72631 Genomic DNA. Translation: CAA96816.1. BK006941 Genomic DNA. Translation: DAA07999.1.
PIR	S64116.
RefSeq	NP_011407.3. NM_001180973.3.
3D structure databases
ProteinModelPortal	P53139.
ModBase	Search...
Protein-protein interaction databases
IntAct	P53139. 11 interactions.
MINT	MINT-2493356.
STRING	4932.YGL108C.
Proteomic databases
PaxDb	P53139.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL108C; YGL108C; YGL108C.
GeneID	852770.
KEGG	sce:YGL108C.
Organism-specific databases
CYGD	YGL108c.
SGD	S000003076. YGL108C.
Phylogenomic databases
eggNOG	NOG272727.
HOGENOM	HOG000142362.
OrthoDB	EOG4DFSZF.
Enzyme and pathway databases
BioCyc	YEAST:G3O-30607-MONOMER.
Gene expression databases
Genevestigator	P53139.
GermOnline	YGL108C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	972233.

Entry information

Entry name

YGK8_YEAST

Accession

Primary (citable) accession number: P53139
Secondary accession number(s): D6VU38

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 29, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program