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P53135 (SLD3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA replication regulator SLD3
Gene names
Name:SLD3
Ordered Locus Names:YGL113W
ORF Names:G2980
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length668 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for loading and maintenance of stable association of CDC45 with chromatin during initiation and elongation of DNA replication. Also involved in temporal regulation of origin firing. Required for the association of PSF1 with origins. Ref.4 Ref.5 Ref.7

Subunit structure

Interacts with CDC45 and SLD7. Ref.4 Ref.7

Subcellular location

Nucleus By similarity.

Miscellaneous

Present with 125 molecules/cell in log phase SD medium.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CDC45Q080325EBI-23925,EBI-4292
SLD7Q084579EBI-23925,EBI-36267

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 668668DNA replication regulator SLD3
PRO_0000071954

Regions

Compositional bias276 – 2794Poly-Leu

Sequences

Sequence LengthMass (Da)Tools
P53135 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 6E59D0DB3C082C28

FASTA66877,298
        10         20         30         40         50         60 
METWEVIASV KEATKGLDLS LDHPLIIKSE DVPSNILQLL QQKNRRQLKH ICMKSRKEYF 

        70         80         90        100        110        120 
LLEEYGPGFW VKWPYNYFNG YSLPERRTEV VTTVERERAK RETLKTWDEL KFKELLHLWS 

       130        140        150        160        170        180 
EEPKGSCKLE KDKDLKLDMN PPDMKGESKI NDYYSDPKEY IESKYYDALF SIHTPLAYFV 

       190        200        210        220        230        240 
KSNLVRLKNT CRTKYGSDSY KIAYQAMLQK FLLSIVQFKD RHDNRLLLEP FSSPIADEKR 

       250        260        270        280        290        300 
KNCLTKFVIQ DENKNSSTIA DLCVVLKSRE IKLQILLLLE IIGLNDLDWN FRDFEKKYKL 

       310        320        330        340        350        360 
KLKKRSLNLT KKGLVRRRSK KKTSEKDKGI ERITTSLDYC EQLDLYLDRA CILDILLSSE 

       370        380        390        400        410        420 
TPNPDAIEAS NGTIQEHKKN ILDKSKEASL VGFINYVLIP YFNKKVPHAV EFIIQKLKGP 

       430        440        450        460        470        480 
SMRPKRALKK VNDSTNVSSP NTVETYNRLS TSQRASRSSI INSVPSSPAL RRVDANLFSR 

       490        500        510        520        530        540 
KSIASPTPEL LNSRTNSNLN EFLESETRSL KRPSQLGRTK SDLTMNHLQK RQFSVSDLST 

       550        560        570        580        590        600 
TRVPNSSTIT LKTPFSHSTI NAYKTMNNSF RRVGKRKDIN ETIRLHERVD SEENVQVQAT 

       610        620        630        640        650        660 
PAVKKRTVTP NKKAQLQSII ESPLNFKDDD THEGRKNTSN ITSTPTNKPP ENSSKRRVRR 


RLFAPEST 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"The genes encoding the transcription factor yTAFII60, the G4p1 protein and a putative glucose transporter are contained in a 12.3 kb DNA fragment on the left arm of Saccharomyces cerevisiae chromosome VII."
Paoluzi S., Minenkova O., Castagnoli L.
Yeast 13:85-91(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 492-668.
[4]"Sld3, which interacts with Cdc45 (Sld4), functions for chromosomal DNA replication in Saccharomyces cerevisiae."
Kamimura Y., Tak Y.-S., Sugino A., Araki H.
EMBO J. 20:2097-2107(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH CDC45.
[5]"GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast."
Takayama Y., Kamimura Y., Okawa M., Muramatsu S., Sugino A., Araki H.
Genes Dev. 17:1153-1165(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Sld7, an Sld3-associated protein required for efficient chromosomal DNA replication in budding yeast."
Tanaka T., Umemori T., Endo S., Muramatsu S., Kanemaki M., Kamimura Y., Obuse C., Araki H.
EMBO J. 30:2019-2030(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SLD7.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z72635 Genomic DNA. Translation: CAA96821.1.
Z72634 Genomic DNA. Translation: CAA96820.1.
X97644 Genomic DNA. Translation: CAA66239.1.
BK006941 Genomic DNA. Translation: DAA07995.1.
PIRS64123.
RefSeqNP_011402.3. NM_001180978.3.

3D structure databases

ProteinModelPortalP53135.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33138. 25 interactions.
DIPDIP-5481N.
IntActP53135. 7 interactions.
MINTMINT-564141.
STRING4932.YGL113W.

Proteomic databases

PaxDbP53135.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGL113W; YGL113W; YGL113W.
GeneID852765.
KEGGsce:YGL113W.

Organism-specific databases

CYGDYGL113w.
SGDS000003081. SLD3.

Phylogenomic databases

eggNOGNOG123149.
HOGENOMHOG000065971.
KOK10731.
OMALKGPSMR.
OrthoDBEOG7G4QQB.

Enzyme and pathway databases

BioCycYEAST:G3O-30611-MONOMER.

Gene expression databases

GenevestigatorP53135.

Family and domain databases

InterProIPR013948. DNA_replication_reg_Sld3.
[Graphical view]
PfamPF08639. SLD3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio972221.

Entry information

Entry nameSLD3_YEAST
AccessionPrimary (citable) accession number: P53135
Secondary accession number(s): D6VU34
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: February 19, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD