P53135 (SLD3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA replication regulator SLD3

Gene names

Name:	SLD3
Ordered Locus Names:	YGL113W
ORF Names:	G2980

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	668 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for loading and maintenance of stable association of CDC45 with chromatin during initiation and elongation of DNA replication. Also involved in temporal regulation of origin firing. Required for the association of PSF1 with origins. Ref.4 Ref.5 Ref.7
Subunit structure	Interacts with CDC45 and SLD7. Ref.4 Ref.7
Subcellular location	Nucleus By similarity.
Miscellaneous	Present with 125 molecules/cell in log phase SD medium.

Ontologies

Keywords
Biological process	Cell cycle DNA replication
Cellular component	Nucleus
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication initiation Inferred from mutant phenotype Ref.4. Source: SGD DNA unwinding involved in replication Inferred from mutant phenotype Ref.4. Source: SGD cell cycle Inferred from electronic annotation. Source: UniProtKB-KW double-strand break repair via break-induced replication Inferred from mutant phenotype PubMed 20516198. Source: SGD
Cellular_component	DNA replication preinitiation complex Inferred from physical interaction Ref.4. Source: SGD nuclear chromatin Inferred from direct assay Ref.4. Source: SGD
Molecular_function	chromatin binding Inferred from direct assay Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 668	668	DNA replication regulator SLD3		PRO_0000071954
Regions
	Compositional bias	276 – 279	4	Poly-Leu

Sequences

	Sequence		Length	Mass (Da)
	P53135 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 6E59D0DB3C082C28	FASTA	668	77,298
	10 20 30 40 50 60 METWEVIASV KEATKGLDLS LDHPLIIKSE DVPSNILQLL QQKNRRQLKH ICMKSRKEYF 70 80 90 100 110 120 LLEEYGPGFW VKWPYNYFNG YSLPERRTEV VTTVERERAK RETLKTWDEL KFKELLHLWS 130 140 150 160 170 180 EEPKGSCKLE KDKDLKLDMN PPDMKGESKI NDYYSDPKEY IESKYYDALF SIHTPLAYFV 190 200 210 220 230 240 KSNLVRLKNT CRTKYGSDSY KIAYQAMLQK FLLSIVQFKD RHDNRLLLEP FSSPIADEKR 250 260 270 280 290 300 KNCLTKFVIQ DENKNSSTIA DLCVVLKSRE IKLQILLLLE IIGLNDLDWN FRDFEKKYKL 310 320 330 340 350 360 KLKKRSLNLT KKGLVRRRSK KKTSEKDKGI ERITTSLDYC EQLDLYLDRA CILDILLSSE 370 380 390 400 410 420 TPNPDAIEAS NGTIQEHKKN ILDKSKEASL VGFINYVLIP YFNKKVPHAV EFIIQKLKGP 430 440 450 460 470 480 SMRPKRALKK VNDSTNVSSP NTVETYNRLS TSQRASRSSI INSVPSSPAL RRVDANLFSR 490 500 510 520 530 540 KSIASPTPEL LNSRTNSNLN EFLESETRSL KRPSQLGRTK SDLTMNHLQK RQFSVSDLST 550 560 570 580 590 600 TRVPNSSTIT LKTPFSHSTI NAYKTMNNSF RRVGKRKDIN ETIRLHERVD SEENVQVQAT 610 620 630 640 650 660 PAVKKRTVTP NKKAQLQSII ESPLNFKDDD THEGRKNTSN ITSTPTNKPP ENSSKRRVRR RLFAPEST

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"The genes encoding the transcription factor yTAFII60, the G4p1 protein and a putative glucose transporter are contained in a 12.3 kb DNA fragment on the left arm of Saccharomyces cerevisiae chromosome VII." Paoluzi S., Minenkova O., Castagnoli L. Yeast 13:85-91(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 492-668.
[4]	"Sld3, which interacts with Cdc45 (Sld4), functions for chromosomal DNA replication in Saccharomyces cerevisiae." Kamimura Y., Tak Y.-S., Sugino A., Araki H. EMBO J. 20:2097-2107(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH CDC45.
[5]	"GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast." Takayama Y., Kamimura Y., Okawa M., Muramatsu S., Sugino A., Araki H. Genes Dev. 17:1153-1165(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Sld7, an Sld3-associated protein required for efficient chromosomal DNA replication in budding yeast." Tanaka T., Umemori T., Endo S., Muramatsu S., Kanemaki M., Kamimura Y., Obuse C., Araki H. EMBO J. 30:2019-2030(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH SLD7.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z72635 Genomic DNA. Translation: CAA96821.1. Z72634 Genomic DNA. Translation: CAA96820.1. X97644 Genomic DNA. Translation: CAA66239.1. BK006941 Genomic DNA. Translation: DAA07995.1.
PIR	S64123.
RefSeq	NP_011402.3. NM_001180978.3.
3D structure databases
ProteinModelPortal	P53135.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5481N.
IntAct	P53135. 3 interactions.
MINT	MINT-564141.
STRING	4932.YGL113W.
Proteomic databases
PaxDb	P53135.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL113W; YGL113W; YGL113W.
GeneID	852765.
KEGG	sce:YGL108C. sce:YGL113W.
Organism-specific databases
CYGD	YGL113w.
SGD	S000003081. SLD3.
Phylogenomic databases
eggNOG	NOG123149.
HOGENOM	HOG000065971.
KO	K10731.
OMA	LKGPSMR.
OrthoDB	EOG44BFBN.
Gene expression databases
Genevestigator	P53135.
GermOnline	YGL113W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013948. DNA_replication_reg_Sld3. [Graphical view]
Pfam	PF08639. SLD3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	972221.

Entry information

Entry name

SLD3_YEAST

Accession

Primary (citable) accession number: P53135
Secondary accession number(s): D6VU34

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program