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P53135

- SLD3_YEAST

UniProt

P53135 - SLD3_YEAST

Protein

DNA replication regulator SLD3

Gene

SLD3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Required for loading and maintenance of stable association of CDC45 with chromatin during initiation and elongation of DNA replication. Also involved in temporal regulation of origin firing. Required for the association of PSF1 with origins.3 Publications

    GO - Molecular functioni

    1. chromatin binding Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. DNA replication initiation Source: SGD
    3. DNA unwinding involved in DNA replication Source: SGD
    4. double-strand break repair via break-induced replication Source: SGD

    Keywords - Biological processi

    Cell cycle, DNA replication

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30611-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA replication regulator SLD3
    Gene namesi
    Name:SLD3
    Ordered Locus Names:YGL113W
    ORF Names:G2980
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGL113w.
    SGDiS000003081. SLD3.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. DNA replication preinitiation complex Source: SGD
    2. nuclear chromatin Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 668668DNA replication regulator SLD3PRO_0000071954Add
    BLAST

    Proteomic databases

    MaxQBiP53135.
    PaxDbiP53135.

    Expressioni

    Gene expression databases

    GenevestigatoriP53135.

    Interactioni

    Subunit structurei

    Interacts with CDC45 and SLD7.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CDC45Q080325EBI-23925,EBI-4292
    SLD7Q084579EBI-23925,EBI-36267

    Protein-protein interaction databases

    BioGridi33138. 27 interactions.
    DIPiDIP-5481N.
    IntActiP53135. 7 interactions.
    MINTiMINT-564141.
    STRINGi4932.YGL113W.

    Structurei

    3D structure databases

    ProteinModelPortaliP53135.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi276 – 2794Poly-Leu

    Phylogenomic databases

    eggNOGiNOG123149.
    HOGENOMiHOG000065971.
    KOiK10731.
    OMAiNRLQKRQ.
    OrthoDBiEOG7G4QQB.

    Family and domain databases

    InterProiIPR013948. DNA_replication_reg_Sld3.
    [Graphical view]
    PfamiPF08639. SLD3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53135-1 [UniParc]FASTAAdd to Basket

    « Hide

    METWEVIASV KEATKGLDLS LDHPLIIKSE DVPSNILQLL QQKNRRQLKH    50
    ICMKSRKEYF LLEEYGPGFW VKWPYNYFNG YSLPERRTEV VTTVERERAK 100
    RETLKTWDEL KFKELLHLWS EEPKGSCKLE KDKDLKLDMN PPDMKGESKI 150
    NDYYSDPKEY IESKYYDALF SIHTPLAYFV KSNLVRLKNT CRTKYGSDSY 200
    KIAYQAMLQK FLLSIVQFKD RHDNRLLLEP FSSPIADEKR KNCLTKFVIQ 250
    DENKNSSTIA DLCVVLKSRE IKLQILLLLE IIGLNDLDWN FRDFEKKYKL 300
    KLKKRSLNLT KKGLVRRRSK KKTSEKDKGI ERITTSLDYC EQLDLYLDRA 350
    CILDILLSSE TPNPDAIEAS NGTIQEHKKN ILDKSKEASL VGFINYVLIP 400
    YFNKKVPHAV EFIIQKLKGP SMRPKRALKK VNDSTNVSSP NTVETYNRLS 450
    TSQRASRSSI INSVPSSPAL RRVDANLFSR KSIASPTPEL LNSRTNSNLN 500
    EFLESETRSL KRPSQLGRTK SDLTMNHLQK RQFSVSDLST TRVPNSSTIT 550
    LKTPFSHSTI NAYKTMNNSF RRVGKRKDIN ETIRLHERVD SEENVQVQAT 600
    PAVKKRTVTP NKKAQLQSII ESPLNFKDDD THEGRKNTSN ITSTPTNKPP 650
    ENSSKRRVRR RLFAPEST 668
    Length:668
    Mass (Da):77,298
    Last modified:October 1, 1996 - v1
    Checksum:i6E59D0DB3C082C28
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72635 Genomic DNA. Translation: CAA96821.1.
    Z72634 Genomic DNA. Translation: CAA96820.1.
    X97644 Genomic DNA. Translation: CAA66239.1.
    BK006941 Genomic DNA. Translation: DAA07995.1.
    PIRiS64123.
    RefSeqiNP_011402.3. NM_001180978.3.

    Genome annotation databases

    EnsemblFungiiYGL113W; YGL113W; YGL113W.
    GeneIDi852765.
    KEGGisce:YGL113W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72635 Genomic DNA. Translation: CAA96821.1 .
    Z72634 Genomic DNA. Translation: CAA96820.1 .
    X97644 Genomic DNA. Translation: CAA66239.1 .
    BK006941 Genomic DNA. Translation: DAA07995.1 .
    PIRi S64123.
    RefSeqi NP_011402.3. NM_001180978.3.

    3D structure databases

    ProteinModelPortali P53135.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33138. 27 interactions.
    DIPi DIP-5481N.
    IntActi P53135. 7 interactions.
    MINTi MINT-564141.
    STRINGi 4932.YGL113W.

    Proteomic databases

    MaxQBi P53135.
    PaxDbi P53135.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL113W ; YGL113W ; YGL113W .
    GeneIDi 852765.
    KEGGi sce:YGL113W.

    Organism-specific databases

    CYGDi YGL113w.
    SGDi S000003081. SLD3.

    Phylogenomic databases

    eggNOGi NOG123149.
    HOGENOMi HOG000065971.
    KOi K10731.
    OMAi NRLQKRQ.
    OrthoDBi EOG7G4QQB.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30611-MONOMER.

    Miscellaneous databases

    NextBioi 972221.

    Gene expression databases

    Genevestigatori P53135.

    Family and domain databases

    InterProi IPR013948. DNA_replication_reg_Sld3.
    [Graphical view ]
    Pfami PF08639. SLD3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "The genes encoding the transcription factor yTAFII60, the G4p1 protein and a putative glucose transporter are contained in a 12.3 kb DNA fragment on the left arm of Saccharomyces cerevisiae chromosome VII."
      Paoluzi S., Minenkova O., Castagnoli L.
      Yeast 13:85-91(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 492-668.
    4. "Sld3, which interacts with Cdc45 (Sld4), functions for chromosomal DNA replication in Saccharomyces cerevisiae."
      Kamimura Y., Tak Y.-S., Sugino A., Araki H.
      EMBO J. 20:2097-2107(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CDC45.
    5. "GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast."
      Takayama Y., Kamimura Y., Okawa M., Muramatsu S., Sugino A., Araki H.
      Genes Dev. 17:1153-1165(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Sld7, an Sld3-associated protein required for efficient chromosomal DNA replication in budding yeast."
      Tanaka T., Umemori T., Endo S., Muramatsu S., Kanemaki M., Kamimura Y., Obuse C., Araki H.
      EMBO J. 30:2019-2030(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SLD7.

    Entry informationi

    Entry nameiSLD3_YEAST
    AccessioniPrimary (citable) accession number: P53135
    Secondary accession number(s): D6VU34
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 125 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3