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Protein

DNA replication regulator SLD3

Gene

SLD3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for loading and maintenance of stable association of CDC45 with chromatin during initiation and elongation of DNA replication. Also involved in temporal regulation of origin firing. Required for the association of PSF1 with origins.3 Publications

GO - Molecular functioni

  • chromatin binding Source: SGD

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • DNA replication initiation Source: SGD
  • DNA unwinding involved in DNA replication Source: SGD
  • double-strand break repair via break-induced replication Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, DNA replication

Enzyme and pathway databases

BioCyciYEAST:G3O-30611-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication regulator SLD3
Gene namesi
Name:SLD3
Ordered Locus Names:YGL113W
ORF Names:G2980
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome VII

Organism-specific databases

CYGDiYGL113w.
EuPathDBiFungiDB:YGL113W.
SGDiS000003081. SLD3.

Subcellular locationi

GO - Cellular componenti

  • chromosome, centromeric region Source: SGD
  • DNA replication preinitiation complex Source: SGD
  • nuclear chromatin Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 668668DNA replication regulator SLD3PRO_0000071954Add
BLAST

Proteomic databases

MaxQBiP53135.
PaxDbiP53135.

Expressioni

Gene expression databases

GenevestigatoriP53135.

Interactioni

Subunit structurei

Interacts with CDC45 and SLD7.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC45Q080325EBI-23925,EBI-4292
SLD7Q084579EBI-23925,EBI-36267

Protein-protein interaction databases

BioGridi33138. 27 interactions.
DIPiDIP-5481N.
IntActiP53135. 7 interactions.
MINTiMINT-564141.
STRINGi4932.YGL113W.

Structurei

Secondary structure

1
668
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi157 – 17014Combined sources
Helixi176 – 1816Combined sources
Helixi183 – 19513Combined sources
Turni197 – 1993Combined sources
Helixi200 – 20910Combined sources
Helixi215 – 2239Combined sources
Helixi226 – 2283Combined sources
Helixi234 – 24714Combined sources
Helixi258 – 28427Combined sources
Helixi288 – 2914Combined sources
Helixi339 – 35820Combined sources
Helixi373 – 3808Combined sources
Helixi390 – 3967Combined sources
Helixi398 – 4025Combined sources
Beta strandi403 – 4064Combined sources
Helixi407 – 41711Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3WI3X-ray2.40A/B/C148-430[»]
ProteinModelPortaliP53135.
SMRiP53135. Positions 154-421.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi276 – 2794Poly-Leu

Phylogenomic databases

eggNOGiNOG123149.
HOGENOMiHOG000065971.
InParanoidiP53135.
KOiK10731.
OMAiLKGPSMR.
OrthoDBiEOG7G4QQB.

Family and domain databases

InterProiIPR013948. DNA_replication_reg_Sld3.
[Graphical view]
PfamiPF08639. SLD3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53135-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METWEVIASV KEATKGLDLS LDHPLIIKSE DVPSNILQLL QQKNRRQLKH
60 70 80 90 100
ICMKSRKEYF LLEEYGPGFW VKWPYNYFNG YSLPERRTEV VTTVERERAK
110 120 130 140 150
RETLKTWDEL KFKELLHLWS EEPKGSCKLE KDKDLKLDMN PPDMKGESKI
160 170 180 190 200
NDYYSDPKEY IESKYYDALF SIHTPLAYFV KSNLVRLKNT CRTKYGSDSY
210 220 230 240 250
KIAYQAMLQK FLLSIVQFKD RHDNRLLLEP FSSPIADEKR KNCLTKFVIQ
260 270 280 290 300
DENKNSSTIA DLCVVLKSRE IKLQILLLLE IIGLNDLDWN FRDFEKKYKL
310 320 330 340 350
KLKKRSLNLT KKGLVRRRSK KKTSEKDKGI ERITTSLDYC EQLDLYLDRA
360 370 380 390 400
CILDILLSSE TPNPDAIEAS NGTIQEHKKN ILDKSKEASL VGFINYVLIP
410 420 430 440 450
YFNKKVPHAV EFIIQKLKGP SMRPKRALKK VNDSTNVSSP NTVETYNRLS
460 470 480 490 500
TSQRASRSSI INSVPSSPAL RRVDANLFSR KSIASPTPEL LNSRTNSNLN
510 520 530 540 550
EFLESETRSL KRPSQLGRTK SDLTMNHLQK RQFSVSDLST TRVPNSSTIT
560 570 580 590 600
LKTPFSHSTI NAYKTMNNSF RRVGKRKDIN ETIRLHERVD SEENVQVQAT
610 620 630 640 650
PAVKKRTVTP NKKAQLQSII ESPLNFKDDD THEGRKNTSN ITSTPTNKPP
660
ENSSKRRVRR RLFAPEST
Length:668
Mass (Da):77,298
Last modified:October 1, 1996 - v1
Checksum:i6E59D0DB3C082C28
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72635 Genomic DNA. Translation: CAA96821.1.
Z72634 Genomic DNA. Translation: CAA96820.1.
X97644 Genomic DNA. Translation: CAA66239.1.
BK006941 Genomic DNA. Translation: DAA07995.1.
PIRiS64123.
RefSeqiNP_011402.3. NM_001180978.3.

Genome annotation databases

EnsemblFungiiYGL113W; YGL113W; YGL113W.
GeneIDi852765.
KEGGisce:YGL113W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72635 Genomic DNA. Translation: CAA96821.1.
Z72634 Genomic DNA. Translation: CAA96820.1.
X97644 Genomic DNA. Translation: CAA66239.1.
BK006941 Genomic DNA. Translation: DAA07995.1.
PIRiS64123.
RefSeqiNP_011402.3. NM_001180978.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3WI3X-ray2.40A/B/C148-430[»]
ProteinModelPortaliP53135.
SMRiP53135. Positions 154-421.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33138. 27 interactions.
DIPiDIP-5481N.
IntActiP53135. 7 interactions.
MINTiMINT-564141.
STRINGi4932.YGL113W.

Proteomic databases

MaxQBiP53135.
PaxDbiP53135.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL113W; YGL113W; YGL113W.
GeneIDi852765.
KEGGisce:YGL113W.

Organism-specific databases

CYGDiYGL113w.
EuPathDBiFungiDB:YGL113W.
SGDiS000003081. SLD3.

Phylogenomic databases

eggNOGiNOG123149.
HOGENOMiHOG000065971.
InParanoidiP53135.
KOiK10731.
OMAiLKGPSMR.
OrthoDBiEOG7G4QQB.

Enzyme and pathway databases

BioCyciYEAST:G3O-30611-MONOMER.

Miscellaneous databases

NextBioi972221.
PROiP53135.

Gene expression databases

GenevestigatoriP53135.

Family and domain databases

InterProiIPR013948. DNA_replication_reg_Sld3.
[Graphical view]
PfamiPF08639. SLD3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "The genes encoding the transcription factor yTAFII60, the G4p1 protein and a putative glucose transporter are contained in a 12.3 kb DNA fragment on the left arm of Saccharomyces cerevisiae chromosome VII."
    Paoluzi S., Minenkova O., Castagnoli L.
    Yeast 13:85-91(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 492-668.
  4. "Sld3, which interacts with Cdc45 (Sld4), functions for chromosomal DNA replication in Saccharomyces cerevisiae."
    Kamimura Y., Tak Y.-S., Sugino A., Araki H.
    EMBO J. 20:2097-2107(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CDC45.
  5. "GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast."
    Takayama Y., Kamimura Y., Okawa M., Muramatsu S., Sugino A., Araki H.
    Genes Dev. 17:1153-1165(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Sld7, an Sld3-associated protein required for efficient chromosomal DNA replication in budding yeast."
    Tanaka T., Umemori T., Endo S., Muramatsu S., Kanemaki M., Kamimura Y., Obuse C., Araki H.
    EMBO J. 30:2019-2030(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SLD7.

Entry informationi

Entry nameiSLD3_YEAST
AccessioniPrimary (citable) accession number: P53135
Secondary accession number(s): D6VU34
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 29, 2015
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 125 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.