Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43

Gene

PRP43

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA.2 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi116 – 1238ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: SGD
  • nucleic acid binding Source: InterPro

GO - Biological processi

  • maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • maturation of SSU-rRNA Source: SGD
  • maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • ribosomal large subunit biogenesis Source: SGD
  • rRNA processing Source: SGD
  • spliceosomal complex disassembly Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30617-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43 (EC:3.6.4.13)
Alternative name(s):
Helicase JA1
Gene namesi
Name:PRP43
Ordered Locus Names:YGL120C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL120C.
SGDiS000003088. PRP43.

Subcellular locationi

GO - Cellular componenti

  • post-mRNA release spliceosomal complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi194 – 1941G → D in PRP43-DN1; dominant-negative phenotype. 1 Publication
Mutagenesisi247 – 2471S → L in PRP43-DN2; dominant-negative phenotype. 1 Publication
Mutagenesisi395 – 3951G → E in PRP43-1; temperature-sensitive. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 767767Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43PRO_0000055145Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei8 – 81PhosphoserineCombined sources
Modified residuei9 – 91PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53131.
PRIDEiP53131.

PTM databases

iPTMnetiP53131.

Interactioni

Subunit structurei

Component of the NTR complex (NTC-related complex), composed of NTR1, NTR2 and PRP43. Interacts with NTR1 and NTR2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
EBP2P360493EBI-505,EBI-6289
LOC1P435863EBI-505,EBI-22906
NET1P470352EBI-505,EBI-25953
NOP12Q082082EBI-505,EBI-35895
NOP15P539273EBI-505,EBI-28853
NOP4P378383EBI-505,EBI-12122
NSA2P400783EBI-505,EBI-22681
PXR1P533353EBI-505,EBI-23652
RPA190P109642EBI-505,EBI-15730
SPP382Q0641112EBI-505,EBI-576
SQS1P538669EBI-505,EBI-29168

Protein-protein interaction databases

BioGridi33131. 178 interactions.
DIPiDIP-5152N.
IntActiP53131. 166 interactions.
MINTiMINT-497996.

Structurei

Secondary structure

1
767
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi9 – 113Combined sources
Turni14 – 174Combined sources
Helixi19 – 3315Combined sources
Turni49 – 524Combined sources
Helixi60 – 689Combined sources
Beta strandi69 – 713Combined sources
Turni73 – 753Combined sources
Helixi81 – 9010Combined sources
Helixi94 – 985Combined sources
Helixi99 – 10810Combined sources
Beta strandi110 – 1156Combined sources
Helixi122 – 13413Combined sources
Helixi136 – 1394Combined sources
Beta strandi142 – 1487Combined sources
Helixi150 – 16314Combined sources
Turni169 – 1713Combined sources
Beta strandi172 – 1765Combined sources
Beta strandi179 – 1813Combined sources
Beta strandi187 – 1926Combined sources
Helixi193 – 20210Combined sources
Beta strandi209 – 2146Combined sources
Helixi217 – 2193Combined sources
Helixi222 – 23716Combined sources
Beta strandi242 – 2487Combined sources
Helixi253 – 2586Combined sources
Beta strandi264 – 2663Combined sources
Beta strandi274 – 2774Combined sources
Helixi286 – 30015Combined sources
Beta strandi305 – 3095Combined sources
Helixi313 – 33422Combined sources
Beta strandi339 – 3446Combined sources
Helixi350 – 3534Combined sources
Helixi354 – 3574Combined sources
Beta strandi364 – 3674Combined sources
Beta strandi370 – 3756Combined sources
Helixi378 – 3814Combined sources
Beta strandi388 – 3936Combined sources
Beta strandi395 – 4039Combined sources
Turni404 – 4074Combined sources
Beta strandi408 – 4158Combined sources
Helixi418 – 4269Combined sources
Helixi427 – 4293Combined sources
Beta strandi430 – 44011Combined sources
Helixi442 – 4476Combined sources
Helixi456 – 4594Combined sources
Helixi463 – 4719Combined sources
Helixi477 – 4793Combined sources
Helixi488 – 50013Combined sources
Helixi512 – 5176Combined sources
Beta strandi520 – 5223Combined sources
Helixi524 – 5329Combined sources
Helixi533 – 5364Combined sources
Helixi539 – 54911Combined sources
Helixi562 – 5709Combined sources
Helixi578 – 58912Combined sources
Helixi592 – 5976Combined sources
Helixi599 – 6057Combined sources
Helixi610 – 62920Combined sources
Helixi644 – 65613Combined sources
Beta strandi659 – 6635Combined sources
Turni665 – 6673Combined sources
Beta strandi670 – 6723Combined sources
Turni673 – 6753Combined sources
Beta strandi678 – 6814Combined sources
Beta strandi692 – 71221Combined sources
Helixi715 – 7217Combined sources
Turni723 – 7253Combined sources
Helixi728 – 7303Combined sources
Helixi735 – 74814Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2XAUX-ray1.90A/B1-767[»]
3KX2X-ray2.20A/B1-767[»]
ProteinModelPortaliP53131.
SMRiP53131. Positions 1-755.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53131.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini103 – 268166Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini293 – 473181Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi215 – 2184DEAH box

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00770000120558.
HOGENOMiHOG000175261.
InParanoidiP53131.
KOiK12820.
OMAiDKYHSTQ.
OrthoDBiEOG092C2SAZ.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53131-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSKRRFSSE HPDPVETSIP EQAAEIAEEL SKQHPLPSEE PLVHHDAGEF
60 70 80 90 100
KGLQRHHTSA EEAQKLEDGK INPFTGREFT PKYVDILKIR RELPVHAQRD
110 120 130 140 150
EFLKLYQNNQ IMVFVGETGS GKTTQIPQFV LFDEMPHLEN TQVACTQPRR
160 170 180 190 200
VAAMSVAQRV AEEMDVKLGE EVGYSIRFEN KTSNKTILKY MTDGMLLREA
210 220 230 240 250
MEDHDLSRYS CIILDEAHER TLATDILMGL LKQVVKRRPD LKIIIMSATL
260 270 280 290 300
DAEKFQRYFN DAPLLAVPGR TYPVELYYTP EFQRDYLDSA IRTVLQIHAT
310 320 330 340 350
EEAGDILLFL TGEDEIEDAV RKISLEGDQL VREEGCGPLS VYPLYGSLPP
360 370 380 390 400
HQQQRIFEPA PESHNGRPGR KVVISTNIAE TSLTIDGIVY VVDPGFSKQK
410 420 430 440 450
VYNPRIRVES LLVSPISKAS AQQRAGRAGR TRPGKCFRLY TEEAFQKELI
460 470 480 490 500
EQSYPEILRS NLSSTVLELK KLGIDDLVHF DFMDPPAPET MMRALEELNY
510 520 530 540 550
LACLDDEGNL TPLGRLASQF PLDPMLAVML IGSFEFQCSQ EILTIVAMLS
560 570 580 590 600
VPNVFIRPTK DKKRADDAKN IFAHPDGDHI TLLNVYHAFK SDEAYEYGIH
610 620 630 640 650
KWCRDHYLNY RSLSAADNIR SQLERLMNRY NLELNTTDYE SPKYFDNIRK
660 670 680 690 700
ALASGFFMQV AKKRSGAKGY ITVKDNQDVL IHPSTVLGHD AEWVIYNEFV
710 720 730 740 750
LTSKNYIRTV TSVRPEWLIE IAPAYYDLSN FQKGDVKLSL ERIKEKVDRL
760
NELKQGKNKK KSKHSKK
Length:767
Mass (Da):87,562
Last modified:October 1, 1996 - v1
Checksum:iDA8A085EC50FF430
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41851 Genomic DNA. Translation: AAB86458.1.
Z72642 Genomic DNA. Translation: CAA96828.1.
BK006941 Genomic DNA. Translation: DAA07989.1.
PIRiS64130.
RefSeqiNP_011395.1. NM_001180985.1.

Genome annotation databases

EnsemblFungiiYGL120C; YGL120C; YGL120C.
GeneIDi852757.
KEGGisce:YGL120C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41851 Genomic DNA. Translation: AAB86458.1.
Z72642 Genomic DNA. Translation: CAA96828.1.
BK006941 Genomic DNA. Translation: DAA07989.1.
PIRiS64130.
RefSeqiNP_011395.1. NM_001180985.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2XAUX-ray1.90A/B1-767[»]
3KX2X-ray2.20A/B1-767[»]
ProteinModelPortaliP53131.
SMRiP53131. Positions 1-755.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33131. 178 interactions.
DIPiDIP-5152N.
IntActiP53131. 166 interactions.
MINTiMINT-497996.

PTM databases

iPTMnetiP53131.

Proteomic databases

MaxQBiP53131.
PRIDEiP53131.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL120C; YGL120C; YGL120C.
GeneIDi852757.
KEGGisce:YGL120C.

Organism-specific databases

EuPathDBiFungiDB:YGL120C.
SGDiS000003088. PRP43.

Phylogenomic databases

GeneTreeiENSGT00770000120558.
HOGENOMiHOG000175261.
InParanoidiP53131.
KOiK12820.
OMAiDKYHSTQ.
OrthoDBiEOG092C2SAZ.

Enzyme and pathway databases

BioCyciYEAST:G3O-30617-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP53131.
PROiP53131.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRP43_YEAST
AccessioniPrimary (citable) accession number: P53131
Secondary accession number(s): D6VU28
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 160 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 16900 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.