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Protein

Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43

Gene

PRP43

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA.2 Publications

Miscellaneous

Present with 16900 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi116 – 123ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: SGD
  • nucleic acid binding Source: InterPro

GO - Biological processi

  • maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • maturation of SSU-rRNA Source: SGD
  • maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • ribosomal large subunit biogenesis Source: SGD
  • rRNA processing Source: SGD
  • spliceosomal complex disassembly Source: SGD

Keywordsi

Molecular functionHelicase, Hydrolase
Biological processmRNA processing, mRNA splicing
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30617-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43 (EC:3.6.4.13)
Alternative name(s):
Helicase JA1
Gene namesi
Name:PRP43
Ordered Locus Names:YGL120C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL120C
SGDiS000003088 PRP43

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi194G → D in PRP43-DN1; dominant-negative phenotype. 1 Publication1
Mutagenesisi247S → L in PRP43-DN2; dominant-negative phenotype. 1 Publication1
Mutagenesisi395G → E in PRP43-1; temperature-sensitive. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000551451 – 767Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43Add BLAST767

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei8PhosphoserineCombined sources1
Modified residuei9PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53131
PaxDbiP53131
PRIDEiP53131

PTM databases

iPTMnetiP53131

Interactioni

Subunit structurei

Component of the NTR complex (NTC-related complex), composed of NTR1, NTR2 and PRP43. Interacts with NTR1 and NTR2. Interacts with SPP382.3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi33131, 457 interactors
ComplexPortaliCPX-1604 Small ribosomal subunit processome, variant 1
CPX-1607 Small ribosomal subunit processome, variant 2
CPX-1608 Small ribosomal subunit processome, variant 3
CPX-1886 Post-mRNA release spliceosomal complex
DIPiDIP-5152N
IntActiP53131, 171 interactors
MINTiP53131
STRINGi4932.YGL120C

Structurei

Secondary structure

1767
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi9 – 11Combined sources3
Turni14 – 17Combined sources4
Helixi19 – 33Combined sources15
Turni49 – 52Combined sources4
Helixi60 – 68Combined sources9
Beta strandi69 – 71Combined sources3
Turni73 – 75Combined sources3
Beta strandi76 – 78Combined sources3
Helixi81 – 90Combined sources10
Helixi94 – 98Combined sources5
Helixi99 – 108Combined sources10
Beta strandi110 – 115Combined sources6
Helixi122 – 134Combined sources13
Helixi136 – 139Combined sources4
Beta strandi142 – 148Combined sources7
Helixi150 – 163Combined sources14
Turni169 – 171Combined sources3
Beta strandi172 – 176Combined sources5
Beta strandi179 – 181Combined sources3
Beta strandi187 – 192Combined sources6
Helixi193 – 202Combined sources10
Beta strandi209 – 214Combined sources6
Helixi217 – 219Combined sources3
Helixi222 – 237Combined sources16
Beta strandi242 – 248Combined sources7
Helixi253 – 258Combined sources6
Beta strandi264 – 266Combined sources3
Beta strandi274 – 277Combined sources4
Helixi286 – 300Combined sources15
Beta strandi305 – 309Combined sources5
Helixi313 – 334Combined sources22
Beta strandi339 – 344Combined sources6
Helixi350 – 353Combined sources4
Helixi354 – 357Combined sources4
Beta strandi364 – 367Combined sources4
Beta strandi370 – 375Combined sources6
Helixi378 – 381Combined sources4
Beta strandi388 – 393Combined sources6
Beta strandi395 – 403Combined sources9
Turni404 – 407Combined sources4
Beta strandi408 – 415Combined sources8
Helixi418 – 426Combined sources9
Helixi427 – 429Combined sources3
Beta strandi430 – 440Combined sources11
Helixi442 – 447Combined sources6
Helixi456 – 459Combined sources4
Helixi463 – 471Combined sources9
Helixi477 – 479Combined sources3
Helixi488 – 500Combined sources13
Helixi512 – 517Combined sources6
Beta strandi520 – 522Combined sources3
Helixi524 – 532Combined sources9
Helixi533 – 536Combined sources4
Helixi539 – 549Combined sources11
Beta strandi559 – 561Combined sources3
Helixi562 – 570Combined sources9
Helixi578 – 589Combined sources12
Helixi592 – 597Combined sources6
Helixi599 – 605Combined sources7
Helixi610 – 629Combined sources20
Helixi644 – 656Combined sources13
Beta strandi659 – 663Combined sources5
Turni665 – 667Combined sources3
Beta strandi670 – 672Combined sources3
Turni673 – 675Combined sources3
Beta strandi678 – 681Combined sources4
Beta strandi692 – 712Combined sources21
Helixi715 – 721Combined sources7
Turni723 – 725Combined sources3
Helixi728 – 730Combined sources3
Helixi735 – 748Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2XAUX-ray1.90A/B1-767[»]
3KX2X-ray2.20A/B1-767[»]
5I8QX-ray4.20A/B1-767[»]
5JPTX-ray2.94A/B1-767[»]
ProteinModelPortaliP53131
SMRiP53131
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53131

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini103 – 268Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST166
Domaini293 – 473Helicase C-terminalPROSITE-ProRule annotationAdd BLAST181

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi215 – 218DEAH box4

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00900000140964
HOGENOMiHOG000175261
InParanoidiP53131
KOiK12820
OMAiQWCVDFA
OrthoDBiEOG092C2SAZ

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR002464 DNA/RNA_helicase_DEAH_CS
IPR011709 DUF1605
IPR007502 Helicase-assoc_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF04408 HA2, 1 hit
PF00271 Helicase_C, 1 hit
PF07717 OB_NTP_bind, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00847 HA2, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00690 DEAH_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit

Sequencei

Sequence statusi: Complete.

P53131-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSKRRFSSE HPDPVETSIP EQAAEIAEEL SKQHPLPSEE PLVHHDAGEF
60 70 80 90 100
KGLQRHHTSA EEAQKLEDGK INPFTGREFT PKYVDILKIR RELPVHAQRD
110 120 130 140 150
EFLKLYQNNQ IMVFVGETGS GKTTQIPQFV LFDEMPHLEN TQVACTQPRR
160 170 180 190 200
VAAMSVAQRV AEEMDVKLGE EVGYSIRFEN KTSNKTILKY MTDGMLLREA
210 220 230 240 250
MEDHDLSRYS CIILDEAHER TLATDILMGL LKQVVKRRPD LKIIIMSATL
260 270 280 290 300
DAEKFQRYFN DAPLLAVPGR TYPVELYYTP EFQRDYLDSA IRTVLQIHAT
310 320 330 340 350
EEAGDILLFL TGEDEIEDAV RKISLEGDQL VREEGCGPLS VYPLYGSLPP
360 370 380 390 400
HQQQRIFEPA PESHNGRPGR KVVISTNIAE TSLTIDGIVY VVDPGFSKQK
410 420 430 440 450
VYNPRIRVES LLVSPISKAS AQQRAGRAGR TRPGKCFRLY TEEAFQKELI
460 470 480 490 500
EQSYPEILRS NLSSTVLELK KLGIDDLVHF DFMDPPAPET MMRALEELNY
510 520 530 540 550
LACLDDEGNL TPLGRLASQF PLDPMLAVML IGSFEFQCSQ EILTIVAMLS
560 570 580 590 600
VPNVFIRPTK DKKRADDAKN IFAHPDGDHI TLLNVYHAFK SDEAYEYGIH
610 620 630 640 650
KWCRDHYLNY RSLSAADNIR SQLERLMNRY NLELNTTDYE SPKYFDNIRK
660 670 680 690 700
ALASGFFMQV AKKRSGAKGY ITVKDNQDVL IHPSTVLGHD AEWVIYNEFV
710 720 730 740 750
LTSKNYIRTV TSVRPEWLIE IAPAYYDLSN FQKGDVKLSL ERIKEKVDRL
760
NELKQGKNKK KSKHSKK
Length:767
Mass (Da):87,562
Last modified:October 1, 1996 - v1
Checksum:iDA8A085EC50FF430
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41851 Genomic DNA Translation: AAB86458.1
Z72642 Genomic DNA Translation: CAA96828.1
BK006941 Genomic DNA Translation: DAA07989.1
PIRiS64130
RefSeqiNP_011395.1, NM_001180985.1

Genome annotation databases

EnsemblFungiiYGL120C; YGL120C; YGL120C
GeneIDi852757
KEGGisce:YGL120C

Similar proteinsi

Entry informationi

Entry nameiPRP43_YEAST
AccessioniPrimary (citable) accession number: P53131
Secondary accession number(s): D6VU28
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 20, 2018
This is version 178 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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