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Protein

Methylenetetrahydrofolate reductase 2

Gene

MET13

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FADBy similarity

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei22Proton donor/acceptorBy similarity1
Binding sitei84FADBy similarity1
Binding sitei116SubstrateBy similarity1
Binding sitei156FADBy similarity1
Binding sitei171FADBy similarity1
Binding sitei178FADBy similarity1
Binding sitei189SubstrateBy similarity1
Binding sitei282SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi22 – 27NADBy similarity6
Nucleotide bindingi54 – 55NAD and FADBy similarity2
Nucleotide bindingi114 – 116FADBy similarity3
Nucleotide bindingi133 – 134FADBy similarity2

GO - Molecular functioni

  • methylenetetrahydrofolate reductase (NAD(P)H) activity Source: SGD

GO - Biological processi

  • methionine biosynthetic process Source: SGD
  • one-carbon metabolic process Source: GO_Central
  • tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NADP

Enzyme and pathway databases

BioCyciYEAST:YGL125W-MONOMER.
BRENDAi1.5.1.20. 984.
ReactomeiR-SCE-196757. Metabolism of folate and pterines.
SABIO-RKP53128.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate reductase 2 (EC:1.5.1.20)
Alternative name(s):
YmL45
Gene namesi
Name:MET13
Synonyms:MET11, MRPL45
Ordered Locus Names:YGL125W
ORF Names:G2882
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL125W.
SGDiS000003093. MET13.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001902561 – 600Methylenetetrahydrofolate reductase 2Add BLAST600

Proteomic databases

MaxQBiP53128.
PRIDEiP53128.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
MET12P461512EBI-11572,EBI-11567

Protein-protein interaction databases

BioGridi33126. 21 interactors.
DIPiDIP-5188N.
IntActiP53128. 2 interactors.
MINTiMINT-478501.

Structurei

3D structure databases

ProteinModelPortaliP53128.
SMRiP53128.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000012490.
HOGENOMiHOG000246234.
InParanoidiP53128.
KOiK00297.
OMAiVVGWGPS.
OrthoDBiEOG092C12V2.

Family and domain databases

CDDicd00537. MTHFR. 1 hit.
Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00677. fadh2_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

P53128-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKITEKLEQH RQTSGKPTYS FEYFVPKTTQ GVQNLYDRMD RMYEASLPQF
60 70 80 90 100
IDITWNAGGG RLSHLSTDLV ATAQSVLGLE TCMHLTCTNM PISMIDDALE
110 120 130 140 150
NAYHSGCQNI LALRGDPPRD AENWTPVEGG FQYAKDLIKY IKSKYGDHFA
160 170 180 190 200
IGVAGYPECH PELPNKDVKL DLEYLKQKID AGGDFIITQM FYDVDNFINW
210 220 230 240 250
CSQVRAAGMD VPIIPGIMPI TTYAAFLRRA QWGQISIPQH FSSRLDPIKD
260 270 280 290 300
DDELVRDIGT NLIVEMCQKL LDSGYVSHLH IYTMNLEKAP LMILERLNIL
310 320 330 340 350
PTESEFNAHP LAVLPWRKSL NPKRKNEEVR PIFWKRRPYS YVARTSQWAV
360 370 380 390 400
DEFPNGRFGD SSSPAFGDLD LCGSDLIRQS ANKCLELWST PTSINDVAFL
410 420 430 440 450
VINYLNGNLK CLPWSDIPIN DEINPIKAHL IELNQHSIIT INSQPQVNGI
460 470 480 490 500
RSNDKIHGWG PKDGYVYQKQ YLEFMLPKTK LPKLIDTLKN NEFLTYFAID
510 520 530 540 550
SQGDLLSNHP DNSKSNAVTW GIFPGREILQ PTIVEKISFL AWKEEFYHIL
560 570 580 590 600
NEWKLNMNKY DKPHSAQFIQ SLIDDYCLVN IVDNDYISPD DQIHSILLSL
Length:600
Mass (Da):68,560
Last modified:October 17, 2006 - v2
Checksum:i470B0EFE4E2D1D75
GO

Sequence cautioni

The sequence CAA63833 differs from that shown. Sequencing errors.Curated
The sequence CAA96833 differs from that shown. Sequencing errors.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti41 – 42RM → LA AA sequence (PubMed:9151978).Curated2
Sequence conflicti48P → A AA sequence (PubMed:9151978).Curated1
Sequence conflicti73A → R in CAA63833 (PubMed:8896269).Curated1
Sequence conflicti152 – 154GVA → RC in AAC99805 (Ref. 5) Curated3
Sequence conflicti183Missing in AAC99805 (Ref. 5) Curated1
Sequence conflicti211V → L in AAC99805 (Ref. 5) Curated1
Sequence conflicti233 – 243GQISIPQHFSS → ANLHPSTFLV in AAC99805 (Ref. 5) CuratedAdd BLAST11
Sequence conflicti266 – 276MCQKLLDSGYV → CVKIARQWLR in AAC99805 (Ref. 5) CuratedAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72647 Genomic DNA. Translation: CAA96833.1. Sequence problems.
X94106 Genomic DNA. Translation: CAA63833.1. Sequence problems.
U24271 Genomic DNA. Translation: AAC99805.1.
BK006941 Genomic DNA. Translation: DAA07984.1.
PIRiS64136.
RefSeqiNP_011390.2. NM_001180990.1.

Genome annotation databases

EnsemblFungiiYGL125W; YGL125W; YGL125W.
GeneIDi852752.
KEGGisce:YGL125W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72647 Genomic DNA. Translation: CAA96833.1. Sequence problems.
X94106 Genomic DNA. Translation: CAA63833.1. Sequence problems.
U24271 Genomic DNA. Translation: AAC99805.1.
BK006941 Genomic DNA. Translation: DAA07984.1.
PIRiS64136.
RefSeqiNP_011390.2. NM_001180990.1.

3D structure databases

ProteinModelPortaliP53128.
SMRiP53128.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33126. 21 interactors.
DIPiDIP-5188N.
IntActiP53128. 2 interactors.
MINTiMINT-478501.

Proteomic databases

MaxQBiP53128.
PRIDEiP53128.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL125W; YGL125W; YGL125W.
GeneIDi852752.
KEGGisce:YGL125W.

Organism-specific databases

EuPathDBiFungiDB:YGL125W.
SGDiS000003093. MET13.

Phylogenomic databases

GeneTreeiENSGT00390000012490.
HOGENOMiHOG000246234.
InParanoidiP53128.
KOiK00297.
OMAiVVGWGPS.
OrthoDBiEOG092C12V2.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciYEAST:YGL125W-MONOMER.
BRENDAi1.5.1.20. 984.
ReactomeiR-SCE-196757. Metabolism of folate and pterines.
SABIO-RKP53128.

Miscellaneous databases

PROiP53128.

Family and domain databases

CDDicd00537. MTHFR. 1 hit.
Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00677. fadh2_euk. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMTHR2_YEAST
AccessioniPrimary (citable) accession number: P53128
Secondary accession number(s): D6VU23, O94090, Q92318
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 17, 2006
Last modified: November 2, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 8600 molecules/cell in log phase SD medium.1 Publication

Caution

Was originally thought to be a mitochondrial ribosomal protein.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.