Reviewed,
UniProtKB/Swiss-Prot P53128 (MTHR2_YEAST)
Last modified
November 25, 2008.
Version 65.
History...
Clusters with 100%,
90%,
50% identity |
Documents (5) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Methylenetetrahydrofolate reductase 2 EC=1.5.1.20 Alternative name(s): YmL45 | ||||||||
| Gene names |
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| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 600 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Catalytic activity | 5-methyltetrahydrofolate + NAD(P)(+) = 5,10-methylenetetrahydrofolate + NAD(P)H. |
| Cofactor | FAD By similarity. |
| Pathway | |
| Miscellaneous | Present with 8600 molecules/cell in log phase SD medium. |
| Sequence similarities | Belongs to the methylenetetrahydrofolate reductase family. |
| Caution | Was originally (Ref.3) thought to be a mitochondrial ribosomal protein. |
| Sequence caution | The sequence CAA63833.1 differs from that shown. Reason: Miscellaneous discrepancy. Sequencing errors. The sequence CAA96833.1 differs from that shown. Reason: Miscellaneous discrepancy. Sequencing errors. |
Ontologies
Keywords | |
|---|---|
| Ligand | FAD Flavoprotein NADP |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome Direct protein sequencing |
Gene Ontology (GO) | |
| Biological process | methionine biosynthetic process Ref.5 Inferred from mutant phenotype. Source: SGD oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | mitochondrion Inferred from direct assay. Source: SGD |
| Molecular function | methylenetetrahydrofolate reductase (NADPH) activity Ref.5 Inferred from electronic annotation. Source: InterPro protein bindingInferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 600 | 600 | Methylenetetrahydrofolate reductase 2 | PRO_0000190256 | |||||
Experimental info | |||||||||
| Sequence conflict | 41 – 42 | 2 | RM → LA AA sequence Ref.3 | ||||||
| Sequence conflict | 48 | 1 | P → A AA sequence Ref.3 | ||||||
| Sequence conflict | 73 | 1 | A → R in CAA63833. Ref.1 | ||||||
| Sequence conflict | 152 – 154 | 3 | GVA → RC in AAC99805. Ref.4 | ||||||
| Sequence conflict | 183 | 1 | Missing in AAC99805. Ref.4 | ||||||
| Sequence conflict | 211 | 1 | V → L in AAC99805. Ref.4 | ||||||
| Sequence conflict | 233 – 243 | 11 | GQISIPQHFSS → ANLHPSTFLV in AAC99805. Ref.4 | ||||||
| Sequence conflict | 266 – 276 | 11 | MCQKLLDSGYV → CVKIARQWLR in AAC99805. Ref.4 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Identification of a putative methylenetetrahydrofolate reductase by sequence analysis of a 6.8 kb DNA fragment of yeast chromosome VII." Tizon B., Rodriguez-Torres A.M., Rodriguez-Belmonte E., Cadahia J.L., Cerdan E. Yeast 12:1047-1051(1996) [PubMed: 8896269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K.Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679. |
| [3] | "Identification and characterization of the genes for mitochondrial ribosomal proteins of Saccharomyces cerevisiae." Kitakawa M., Graack H.-R., Grohmann L., Goldschmidt-Reisin S., Herfurth E., Wittmann-Liebold B., Nishimura T., Isono K. Eur. J. Biochem. 245:449-456(1997) [PubMed: 9151978] [Abstract] Cited for: PROTEIN SEQUENCE OF 39-49. |
| [4] | Housen I., Lafontaine D., Belot N., Vandenhaute J. Submitted (APR-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 120-286. Strain: ATCC 28383 / FL100 / VTT C-80102. |
| [5] | "Saccharomyces cerevisiae expresses two genes encoding isozymes of methylenetetrahydrofolate reductase." Raymond R.K., Kastanos E.K., Appling D.R. Arch. Biochem. Biophys. 372:300-308(1999) [PubMed: 10600168] [Abstract] Cited for: FUNCTION. |
| [6] | "Metabolic engineering in yeast demonstrates that S-adenosylmethionine controls flux through the methylenetetrahydrofolate reductase reaction in vivo." Roje S., Chan S.Y., Kaplan F., Raymond R.K., Horne D.W., Appling D.R., Hanson A.D. J. Biol. Chem. 277:4056-4061(2002) [PubMed: 11729203] [Abstract] Cited for: FUNCTION. |
| [7] | Erratum Roje S., Chan S.Y., Kaplan F., Raymond R.K., Horne D.W., Appling D.R., Hanson A.D. J. Biol. Chem. 277:36904-36904(2002) Cited for: SEQUENCE REVISION TO 176-181; 197-198 AND 230. |
| [8] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| Z72647 Genomic DNA. Translation: CAA96833.1. Sequence problems. X94106 Genomic DNA. Translation: CAA63833.1. Sequence problems. U24271 Genomic DNA. Translation: AAC99805.1. | |
| PIR | S64136. |
| RefSeq | NP_011390.2. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1B5T based on UniProtKB P00394. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:5188N. |
| IntAct | P53128. |
Proteomic databases | |
| PeptideAtlas | P53128. |
Genome annotation databases | |
| Ensembl | YGL125W. Saccharomyces cerevisiae. [Contig view] |
| GeneID | 852752. |
| GenomeReviews | Gene locus YGL125W in contig Y13135_GR. |
| KEGG | sce:YGL125W. |
| NMPDR | fig|4932.3.peg.2494. |
Organism-specific databases | |
| CYGD | YGL125w. |
| SGD | S000003093. MET13. |
| Yeast-GFP | Search... |
Phylogenomic databases | |
| HOGENOM | P53128. |
Gene expression databases | |
| ArrayExpress | P53128. |
| GermOnline | YGL125W. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR004621. Fadh2_euk. IPR003171. Mehydrof_redctse. [Graphical view] |
| Pfam | PF02219. MTHFR. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR00677. fadh2_euk. 1 hit. |
| ProtoNet | Search... |
Other Resources | |
| LinkHub | P53128. |
| NextBio | 972186. |
Entry information
| Entry name | MTHR2_YEAST | ||||||||
| Accession | Primary (citable) accession number: P53128 Secondary accession number(s): O94090, Q92318 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| UniProtKB secondary accession numbers Index of UniProtKB secondary accession numbers |
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome VII Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names |

Clusters with


