Reviewed, UniProtKB/Swiss-Prot P53128 (MTHR2_YEAST)

Names and origin

Protein names

Recommended name:
    Methylenetetrahydrofolate reductase 2
    EC=1.5.1.20
Alternative name(s):
    YmL45

Gene names

Name:	MET13
Synonyms:	MET11, MRPL45
Ordered Locus Names:	YGL125W
ORF Names:	G2882

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	600 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	5-methyltetrahydrofolate + NAD(P)(+) = 5,10-methylenetetrahydrofolate + NAD(P)H.
Cofactor	FAD By similarity.
Pathway	One-carbon metabolism; tetrahydrofolate pathway.
Miscellaneous	Present with 8600 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the methylenetetrahydrofolate reductase family.
Caution	Was originally (Ref.3) thought to be a mitochondrial ribosomal protein.
Sequence caution	The sequence CAA63833.1 differs from that shown. Reason: Miscellaneous discrepancy. Sequencing errors. The sequence CAA96833.1 differs from that shown. Reason: Miscellaneous discrepancy. Sequencing errors.

Ontologies

Keywords
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	methionine biosynthetic process Ref.5 Inferred from mutant phenotype. Source: SGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from direct assay. Source: SGD
Molecular function	methylenetetrahydrofolate reductase (NADPH) activity Ref.5 Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MET12	P46151	1	EBI-11572,EBI-11567

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 600	600	Methylenetetrahydrofolate reductase 2		PRO_0000190256
Experimental info
	Sequence conflict	41 – 42	2	RM → LA AA sequence Ref.3
	Sequence conflict	48	1	P → A AA sequence Ref.3
	Sequence conflict	73	1	A → R in CAA63833. Ref.1
	Sequence conflict	152 – 154	3	GVA → RC in AAC99805. Ref.4
	Sequence conflict	183	1	Missing in AAC99805. Ref.4
	Sequence conflict	211	1	V → L in AAC99805. Ref.4
	Sequence conflict	233 – 243	11	GQISIPQHFSS → ANLHPSTFLV in AAC99805. Ref.4
	Sequence conflict	266 – 276	11	MCQKLLDSGYV → CVKIARQWLR in AAC99805. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P53128-1 [UniParc]. Last modified October 17, 2006. Version 2. Checksum: 470B0EFE4E2D1D75	FASTA	600	68,560
	10 20 30 40 50 60 MKITEKLEQH RQTSGKPTYS FEYFVPKTTQ GVQNLYDRMD RMYEASLPQF IDITWNAGGG 70 80 90 100 110 120 RLSHLSTDLV ATAQSVLGLE TCMHLTCTNM PISMIDDALE NAYHSGCQNI LALRGDPPRD 130 140 150 160 170 180 AENWTPVEGG FQYAKDLIKY IKSKYGDHFA IGVAGYPECH PELPNKDVKL DLEYLKQKID 190 200 210 220 230 240 AGGDFIITQM FYDVDNFINW CSQVRAAGMD VPIIPGIMPI TTYAAFLRRA QWGQISIPQH 250 260 270 280 290 300 FSSRLDPIKD DDELVRDIGT NLIVEMCQKL LDSGYVSHLH IYTMNLEKAP LMILERLNIL 310 320 330 340 350 360 PTESEFNAHP LAVLPWRKSL NPKRKNEEVR PIFWKRRPYS YVARTSQWAV DEFPNGRFGD 370 380 390 400 410 420 SSSPAFGDLD LCGSDLIRQS ANKCLELWST PTSINDVAFL VINYLNGNLK CLPWSDIPIN 430 440 450 460 470 480 DEINPIKAHL IELNQHSIIT INSQPQVNGI RSNDKIHGWG PKDGYVYQKQ YLEFMLPKTK 490 500 510 520 530 540 LPKLIDTLKN NEFLTYFAID SQGDLLSNHP DNSKSNAVTW GIFPGREILQ PTIVEKISFL 550 560 570 580 590 600 AWKEEFYHIL NEWKLNMNKY DKPHSAQFIQ SLIDDYCLVN IVDNDYISPD DQIHSILLSL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a putative methylenetetrahydrofolate reductase by sequence analysis of a 6.8 kb DNA fragment of yeast chromosome VII." Tizon B., Rodriguez-Torres A.M., Rodriguez-Belmonte E., Cadahia J.L., Cerdan E. Yeast 12:1047-1051(1996) [PubMed: 8896269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Identification and characterization of the genes for mitochondrial ribosomal proteins of Saccharomyces cerevisiae." Kitakawa M., Graack H.-R., Grohmann L., Goldschmidt-Reisin S., Herfurth E., Wittmann-Liebold B., Nishimura T., Isono K. Eur. J. Biochem. 245:449-456(1997) [PubMed: 9151978] [Abstract] Cited for: PROTEIN SEQUENCE OF 39-49.
[4]	Housen I., Lafontaine D., Belot N., Vandenhaute J. Submitted (APR-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 120-286. Strain: ATCC 28383 / FL100 / VTT C-80102.
[5]	"Saccharomyces cerevisiae expresses two genes encoding isozymes of methylenetetrahydrofolate reductase." Raymond R.K., Kastanos E.K., Appling D.R. Arch. Biochem. Biophys. 372:300-308(1999) [PubMed: 10600168] [Abstract] Cited for: FUNCTION.
[6]	"Metabolic engineering in yeast demonstrates that S-adenosylmethionine controls flux through the methylenetetrahydrofolate reductase reaction in vivo." Roje S., Chan S.Y., Kaplan F., Raymond R.K., Horne D.W., Appling D.R., Hanson A.D. J. Biol. Chem. 277:4056-4061(2002) [PubMed: 11729203] [Abstract] Cited for: FUNCTION.
[7]	Erratum Roje S., Chan S.Y., Kaplan F., Raymond R.K., Horne D.W., Appling D.R., Hanson A.D. J. Biol. Chem. 277:36904-36904(2002) Cited for: SEQUENCE REVISION TO 176-181; 197-198 AND 230.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z72647 Genomic DNA. Translation: CAA96833.1. Sequence problems. X94106 Genomic DNA. Translation: CAA63833.1. Sequence problems. U24271 Genomic DNA. Translation: AAC99805.1.
PIR	S64136.
RefSeq	NP_011390.2.
3D structure databases
HSSP	HSSP built from PDB template 1B5T based on UniProtKB P00394.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5188N.
IntAct	P53128.
Proteomic databases
PeptideAtlas	P53128.
Genome annotation databases
Ensembl	YGL125W. Saccharomyces cerevisiae. [Contig view]
GeneID	852752.
GenomeReviews	Gene locus YGL125W in contig Y13135_GR.
KEGG	sce:YGL125W.
NMPDR	fig|4932.3.peg.2494.
Organism-specific databases
CYGD	YGL125w.
SGD	S000003093. MET13.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P53128.
Gene expression databases
ArrayExpress	P53128.
GermOnline	YGL125W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004621. Fadh2_euk. IPR003171. Mehydrof_redctse. [Graphical view]
Pfam	PF02219. MTHFR. 1 hit. [Graphical view]
TIGRFAMs	TIGR00677. fadh2_euk. 1 hit.
ProtoNet	Search...
Other Resources
LinkHub	P53128.
NextBio	972186.

Entry information

Entry name

MTHR2_YEAST

Accession

Primary (citable) accession number: P53128
Secondary accession number(s): O94090, Q92318

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 17, 2006
Last modified:	November 25, 2008
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)