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P53125 (ITC1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Imitation switch two complex protein 1
Gene names
Name:ITC1
Ordered Locus Names:YGL133W
ORF Names:G2842
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1264 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions as component of the ISW2 complex, which acts in remodeling the chromatin by catalyzing an ATP-dependent alteration in the structure of nucleosomal DNA. THe ISW2 complex is involved in coordinating transcriptional repression and in inheritance of telomeric silencing. It is involved in repression of MAT a-specific genes, INO1, and early meiotic genes during mitotic growth dependent upon transcription factor UME6 and in a parallel pathway to the RPD3-SIN3 histone deacetylase complex. ITC1 is required for nucleosome-stimulated ATPase activity and chromatin-remodeling activity of the complex. Required for the repression of MATa a-specific genes. Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.10

Subunit structure

Component of the ISW2 complex, which at least consists of ISW2, ITC1, DLS1 and DPB4. May form a stable subcomplex with ISW2. Ref.10 Ref.11

Subcellular location

Nucleus Ref.6.

Miscellaneous

Present with 2610 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 DDT domain.

Contains 1 WAC domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ISW2Q087733EBI-23967,EBI-31118

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12641264Imitation switch two complex protein 1
PRO_0000084270

Regions

Domain23 – 130108WAC
Domain423 – 48361DDT

Sequences

Sequence LengthMass (Da)Tools
P53125 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 45E4CF8835C7C746

FASTA1,264145,643
        10         20         30         40         50         60 
MVLYKRKPIL LPDPKPLPLD LNVQVWHIEE TGEWFSSYEE FLERFDFYTR HHFTCEITGT 

        70         80         90        100        110        120 
SCLTFFQALD SEETQFKYVE DRFPLKLREP VARFLHFNGI RRLDALVEKV YARFKNDFFP 

       130        140        150        160        170        180 
GEVVYLRKQK DSSTTSSNSQ QSTPQPDDMV EINSVGNPGL PQYQYQRRYV IKEKVQFNAT 

       190        200        210        220        230        240 
INPESREIVM PAHTKYMLIE EAASSNKSFI VDQGQIYRDR STFTKHLIKC FFKITLQRAS 

       250        260        270        280        290        300 
SKMGAPWCVK PEYLAMYGLT MEWPKDMLKY KEDEPVVARR SNSANVSSPE SEKNKRQSKS 

       310        320        330        340        350        360 
SGKSNTSNDA SNKKETKKKR KPTEVNDSEN NSSEEDKKKG QNVTSETHSK KRKKEANEEP 

       370        380        390        400        410        420 
NTENVESVPT PANAEPQAVT ITSIMDDLAL PYQHPPNIFP NLTYYNEKLE CISLGSTKLS 

       430        440        450        460        470        480 
RPFDSFGKLL QAYQFLNTFG SKICLSHFSL DQFITSLKCT DPYELKGEVV LVNIRTQTSK 

       490        500        510        520        530        540 
EQEIENNGLP MKNKAETTTE EDSENPSDWQ RNSFIRDMIM KRNSDKVEYK IVHDDPASDD 

       550        560        570        580        590        600 
ILDNINHNGS ALLIEVFTAL LRLFINEEGD WSCIVVENWI IDDKGVLMER KDERGEGEAK 

       610        620        630        640        650        660 
QKRNAHGYFL QDKEKIDNLK DTLKENATEV QKESDAKNET NSESDSKSDS DSEERDPKLE 

       670        680        690        700        710        720 
KCLNYRNVNW IERLTKRQFN NSYWLIILLG VLEDCRHLPM YTEFIDSFIE KIIPKDISAT 

       730        740        750        760        770        780 
QLPKQLWRNF CRKLSFSDKV NALWILVDLV SHFSPDIKAA VDDSMELCGQ IRSERFKVAR 

       790        800        810        820        830        840 
ELKTEAAVLS NLQGDLQAIQ EKLNKTDENT PSADGADKKD DSESNSEPID LIIIEKKQKL 

       850        860        870        880        890        900 
IEEQDKKVQA LQSDKNFLDN CLFENDLQRL KPLGLDRYGN RYFWLDHNGV PFPQYPAGMN 

       910        920        930        940        950        960 
ETPKSNNSLS YHSGRLLIQG PKASSAKFFL NVSDEQLSNW QKIRNSEGIS EATREVFGIS 

       970        980        990       1000       1010       1020 
KTSSGSYNYV ENGIEVELLD SNDRVNPLIE LTPIQKKIMD ETPSRLLLSP DQWYCIDKLE 

      1030       1040       1050       1060       1070       1080 
DLSRIMDWLD NWGRKEHDLL RQIRPIMERI KSSLSLRDHA LSLTAFTKNE EKLLKELENN 

      1090       1100       1110       1120       1130       1140 
EFTENELNVD SMDVDDKNSG VKSEVDVQVD AEEKREAVID EKLEVIADEL MKLDDSSKTR 

      1150       1160       1170       1180       1190       1200 
NVLNRIQELE DQRDELLEQK KSIINSQRPG ARILARSERK RTKISRGNKV NKQIEILTDL 

      1210       1220       1230       1240       1250       1260 
VNYRHFKAME DVIAWKNVLA NSIWGSSLRK NASGNKRSGV IETVDDKLKD IVGQTSRTVT 


PAPN 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of a 14.6 kb DNA fragment of Saccharomyces cerevisiae chromosome VII reveals SEC27, SSM1b, a putative S-adenosylmethionine-dependent enzyme and six new open reading frames."
Escribano V., Eraso P., Portillo F., Mazon M.J.
Yeast 12:887-892(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"The Isw2 chromatin remodeling complex represses early meiotic genes upon recruitment by Ume6p."
Goldmark J.P., Fazzio T.G., Estep P.W., Church G.M., Tsukiyama T.
Cell 103:423-433(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE ISW2 COMPLEX.
[5]"The Saccharomyces cerevisiae Isw2p-Itc1p complex represses INO1 expression and maintains cell morphology."
Sugiyama M., Nikawa J.
J. Bacteriol. 183:4985-4993(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Interactions of Isw2 chromatin remodeling complex with nucleosomal arrays: analyses using recombinant yeast histones and immobilized templates."
Gelbart M.E., Rechsteiner T., Richmond T.J., Tsukiyama T.
Mol. Cell. Biol. 21:2098-2106(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE ISW2 COMPLEX, INTERACTION WITH ISW2, SUBCELLULAR LOCATION.
[7]"Widespread collaboration of Isw2 and Sin3-Rpd3 chromatin remodeling complexes in transcriptional repression."
Fazzio T.G., Kooperberg C., Goldmark J.P., Neal C., Basom R., Delrow J., Tsukiyama T.
Mol. Cell. Biol. 21:6450-6460(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE ISW2 COMPLEX.
[8]"Cell-type-dependent repression of yeast a-specific genes requires Itc1p, a subunit of the Isw2p-Itc1p chromatin remodelling complex."
Ruiz C., Escribano V., Morgado E., Molina M., Mazon M.J.
Microbiology 149:341-351(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]"Noncompetitive counteractions of DNA polymerase epsilon and ISW2/yCHRAC for epigenetic inheritance of telomere position effect in Saccharomyces cerevisiae."
Iida T., Araki H.
Mol. Cell. Biol. 24:217-227(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE ISW2 COMPLEX, FUNCTION OF THE ISW2 COMPLEX.
[11]"Histone fold protein Dls1p is required for Isw2-dependent chromatin remodeling in vivo."
McConnell A.D., Gelbart M.E., Tsukiyama T.
Mol. Cell. Biol. 24:2605-2613(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE ISW2 COMPLEX.
[12]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z72655 Genomic DNA. Translation: CAA96844.1.
BK006941 Genomic DNA. Translation: DAA07977.1.
PIRS64146.
RefSeqNP_011382.1. NM_001180998.1.

3D structure databases

ProteinModelPortalP53125.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33119. 140 interactions.
DIPDIP-6737N.
IntActP53125. 36 interactions.
MINTMINT-469555.
STRING4932.YGL133W.

Proteomic databases

PaxDbP53125.
PeptideAtlasP53125.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGL133W; YGL133W; YGL133W.
GeneID852744.
KEGGsce:YGL133W.

Organism-specific databases

CYGDYGL133w.
SGDS000003101. ITC1.

Phylogenomic databases

eggNOGNOG302161.
GeneTreeENSGT00530000066815.
HOGENOMHOG000065973.
OMAQIYRDRS.
OrthoDBEOG7TTQHC.

Enzyme and pathway databases

BioCycYEAST:G3O-30628-MONOMER.

Gene expression databases

GenevestigatorP53125.

Family and domain databases

InterProIPR004022. DDT_dom.
IPR018500. DDT_dom_subgr.
IPR018501. DDT_dom_superfamily.
IPR028941. WHIM2_dom.
IPR013136. WSTF_Acf1_Cbp146.
[Graphical view]
PfamPF02791. DDT. 1 hit.
PF10537. WAC_Acf1_DNA_bd. 1 hit.
PF15613. WHIM2. 1 hit.
[Graphical view]
SMARTSM00571. DDT. 1 hit.
[Graphical view]
PROSITEPS50827. DDT. 1 hit.
PS51136. WAC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio972165.

Entry information

Entry nameITC1_YEAST
AccessionPrimary (citable) accession number: P53125
Secondary accession number(s): D6VU16
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families