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P53125

- ITC1_YEAST

UniProt

P53125 - ITC1_YEAST

Protein

Imitation switch two complex protein 1

Gene

ITC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Functions as component of the ISW2 complex, which acts in remodeling the chromatin by catalyzing an ATP-dependent alteration in the structure of nucleosomal DNA. THe ISW2 complex is involved in coordinating transcriptional repression and in inheritance of telomeric silencing. It is involved in repression of MAT a-specific genes, INO1, and early meiotic genes during mitotic growth dependent upon transcription factor UME6 and in a parallel pathway to the RPD3-SIN3 histone deacetylase complex. ITC1 is required for nucleosome-stimulated ATPase activity and chromatin-remodeling activity of the complex. Required for the repression of MATa a-specific genes.6 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. chromatin remodeling Source: SGD
    2. chromatin silencing at telomere Source: SGD
    3. negative regulation of transcription from RNA polymerase II promoter by pheromones Source: SGD
    4. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Chromatin regulator, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30628-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Imitation switch two complex protein 1
    Gene namesi
    Name:ITC1
    Ordered Locus Names:YGL133W
    ORF Names:G2842
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGL133w.
    SGDiS000003101. ITC1.

    Subcellular locationi

    Nucleus 1 PublicationPROSITE-ProRule annotation

    GO - Cellular componenti

    1. CHRAC Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12641264Imitation switch two complex protein 1PRO_0000084270Add
    BLAST

    Proteomic databases

    MaxQBiP53125.
    PaxDbiP53125.
    PeptideAtlasiP53125.

    Expressioni

    Gene expression databases

    GenevestigatoriP53125.

    Interactioni

    Subunit structurei

    Component of the ISW2 complex, which at least consists of ISW2, ITC1, DLS1 and DPB4. May form a stable subcomplex with ISW2.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ISW2Q087733EBI-23967,EBI-31118

    Protein-protein interaction databases

    BioGridi33119. 140 interactions.
    DIPiDIP-6737N.
    IntActiP53125. 36 interactions.
    MINTiMINT-469555.
    STRINGi4932.YGL133W.

    Structurei

    3D structure databases

    ProteinModelPortaliP53125.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini23 – 130108WACPROSITE-ProRule annotationAdd
    BLAST
    Domaini423 – 48361DDTPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 DDT domain.PROSITE-ProRule annotation
    Contains 1 WAC domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG302161.
    GeneTreeiENSGT00530000066815.
    HOGENOMiHOG000065973.
    OMAiQIYRDRS.
    OrthoDBiEOG7TTQHC.

    Family and domain databases

    InterProiIPR004022. DDT_dom.
    IPR018500. DDT_dom_subgr.
    IPR018501. DDT_dom_superfamily.
    IPR028941. WHIM2_dom.
    IPR013136. WSTF_Acf1_Cbp146.
    [Graphical view]
    PfamiPF02791. DDT. 1 hit.
    PF10537. WAC_Acf1_DNA_bd. 1 hit.
    PF15613. WHIM2. 1 hit.
    [Graphical view]
    SMARTiSM00571. DDT. 1 hit.
    [Graphical view]
    PROSITEiPS50827. DDT. 1 hit.
    PS51136. WAC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53125-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVLYKRKPIL LPDPKPLPLD LNVQVWHIEE TGEWFSSYEE FLERFDFYTR     50
    HHFTCEITGT SCLTFFQALD SEETQFKYVE DRFPLKLREP VARFLHFNGI 100
    RRLDALVEKV YARFKNDFFP GEVVYLRKQK DSSTTSSNSQ QSTPQPDDMV 150
    EINSVGNPGL PQYQYQRRYV IKEKVQFNAT INPESREIVM PAHTKYMLIE 200
    EAASSNKSFI VDQGQIYRDR STFTKHLIKC FFKITLQRAS SKMGAPWCVK 250
    PEYLAMYGLT MEWPKDMLKY KEDEPVVARR SNSANVSSPE SEKNKRQSKS 300
    SGKSNTSNDA SNKKETKKKR KPTEVNDSEN NSSEEDKKKG QNVTSETHSK 350
    KRKKEANEEP NTENVESVPT PANAEPQAVT ITSIMDDLAL PYQHPPNIFP 400
    NLTYYNEKLE CISLGSTKLS RPFDSFGKLL QAYQFLNTFG SKICLSHFSL 450
    DQFITSLKCT DPYELKGEVV LVNIRTQTSK EQEIENNGLP MKNKAETTTE 500
    EDSENPSDWQ RNSFIRDMIM KRNSDKVEYK IVHDDPASDD ILDNINHNGS 550
    ALLIEVFTAL LRLFINEEGD WSCIVVENWI IDDKGVLMER KDERGEGEAK 600
    QKRNAHGYFL QDKEKIDNLK DTLKENATEV QKESDAKNET NSESDSKSDS 650
    DSEERDPKLE KCLNYRNVNW IERLTKRQFN NSYWLIILLG VLEDCRHLPM 700
    YTEFIDSFIE KIIPKDISAT QLPKQLWRNF CRKLSFSDKV NALWILVDLV 750
    SHFSPDIKAA VDDSMELCGQ IRSERFKVAR ELKTEAAVLS NLQGDLQAIQ 800
    EKLNKTDENT PSADGADKKD DSESNSEPID LIIIEKKQKL IEEQDKKVQA 850
    LQSDKNFLDN CLFENDLQRL KPLGLDRYGN RYFWLDHNGV PFPQYPAGMN 900
    ETPKSNNSLS YHSGRLLIQG PKASSAKFFL NVSDEQLSNW QKIRNSEGIS 950
    EATREVFGIS KTSSGSYNYV ENGIEVELLD SNDRVNPLIE LTPIQKKIMD 1000
    ETPSRLLLSP DQWYCIDKLE DLSRIMDWLD NWGRKEHDLL RQIRPIMERI 1050
    KSSLSLRDHA LSLTAFTKNE EKLLKELENN EFTENELNVD SMDVDDKNSG 1100
    VKSEVDVQVD AEEKREAVID EKLEVIADEL MKLDDSSKTR NVLNRIQELE 1150
    DQRDELLEQK KSIINSQRPG ARILARSERK RTKISRGNKV NKQIEILTDL 1200
    VNYRHFKAME DVIAWKNVLA NSIWGSSLRK NASGNKRSGV IETVDDKLKD 1250
    IVGQTSRTVT PAPN 1264
    Length:1,264
    Mass (Da):145,643
    Last modified:October 1, 1996 - v1
    Checksum:i45E4CF8835C7C746
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72655 Genomic DNA. Translation: CAA96844.1.
    BK006941 Genomic DNA. Translation: DAA07977.1.
    PIRiS64146.
    RefSeqiNP_011382.1. NM_001180998.1.

    Genome annotation databases

    EnsemblFungiiYGL133W; YGL133W; YGL133W.
    GeneIDi852744.
    KEGGisce:YGL133W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72655 Genomic DNA. Translation: CAA96844.1 .
    BK006941 Genomic DNA. Translation: DAA07977.1 .
    PIRi S64146.
    RefSeqi NP_011382.1. NM_001180998.1.

    3D structure databases

    ProteinModelPortali P53125.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33119. 140 interactions.
    DIPi DIP-6737N.
    IntActi P53125. 36 interactions.
    MINTi MINT-469555.
    STRINGi 4932.YGL133W.

    Proteomic databases

    MaxQBi P53125.
    PaxDbi P53125.
    PeptideAtlasi P53125.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL133W ; YGL133W ; YGL133W .
    GeneIDi 852744.
    KEGGi sce:YGL133W.

    Organism-specific databases

    CYGDi YGL133w.
    SGDi S000003101. ITC1.

    Phylogenomic databases

    eggNOGi NOG302161.
    GeneTreei ENSGT00530000066815.
    HOGENOMi HOG000065973.
    OMAi QIYRDRS.
    OrthoDBi EOG7TTQHC.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30628-MONOMER.

    Miscellaneous databases

    NextBioi 972165.

    Gene expression databases

    Genevestigatori P53125.

    Family and domain databases

    InterProi IPR004022. DDT_dom.
    IPR018500. DDT_dom_subgr.
    IPR018501. DDT_dom_superfamily.
    IPR028941. WHIM2_dom.
    IPR013136. WSTF_Acf1_Cbp146.
    [Graphical view ]
    Pfami PF02791. DDT. 1 hit.
    PF10537. WAC_Acf1_DNA_bd. 1 hit.
    PF15613. WHIM2. 1 hit.
    [Graphical view ]
    SMARTi SM00571. DDT. 1 hit.
    [Graphical view ]
    PROSITEi PS50827. DDT. 1 hit.
    PS51136. WAC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of a 14.6 kb DNA fragment of Saccharomyces cerevisiae chromosome VII reveals SEC27, SSM1b, a putative S-adenosylmethionine-dependent enzyme and six new open reading frames."
      Escribano V., Eraso P., Portillo F., Mazon M.J.
      Yeast 12:887-892(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The Isw2 chromatin remodeling complex represses early meiotic genes upon recruitment by Ume6p."
      Goldmark J.P., Fazzio T.G., Estep P.W., Church G.M., Tsukiyama T.
      Cell 103:423-433(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE ISW2 COMPLEX.
    5. "The Saccharomyces cerevisiae Isw2p-Itc1p complex represses INO1 expression and maintains cell morphology."
      Sugiyama M., Nikawa J.
      J. Bacteriol. 183:4985-4993(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Interactions of Isw2 chromatin remodeling complex with nucleosomal arrays: analyses using recombinant yeast histones and immobilized templates."
      Gelbart M.E., Rechsteiner T., Richmond T.J., Tsukiyama T.
      Mol. Cell. Biol. 21:2098-2106(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE ISW2 COMPLEX, INTERACTION WITH ISW2, SUBCELLULAR LOCATION.
    7. "Widespread collaboration of Isw2 and Sin3-Rpd3 chromatin remodeling complexes in transcriptional repression."
      Fazzio T.G., Kooperberg C., Goldmark J.P., Neal C., Basom R., Delrow J., Tsukiyama T.
      Mol. Cell. Biol. 21:6450-6460(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE ISW2 COMPLEX.
    8. "Cell-type-dependent repression of yeast a-specific genes requires Itc1p, a subunit of the Isw2p-Itc1p chromatin remodelling complex."
      Ruiz C., Escribano V., Morgado E., Molina M., Mazon M.J.
      Microbiology 149:341-351(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    10. "Noncompetitive counteractions of DNA polymerase epsilon and ISW2/yCHRAC for epigenetic inheritance of telomere position effect in Saccharomyces cerevisiae."
      Iida T., Araki H.
      Mol. Cell. Biol. 24:217-227(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE ISW2 COMPLEX, FUNCTION OF THE ISW2 COMPLEX.
    11. "Histone fold protein Dls1p is required for Isw2-dependent chromatin remodeling in vivo."
      McConnell A.D., Gelbart M.E., Tsukiyama T.
      Mol. Cell. Biol. 24:2605-2613(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE ISW2 COMPLEX.
    12. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiITC1_YEAST
    AccessioniPrimary (citable) accession number: P53125
    Secondary accession number(s): D6VU16
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2610 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3