Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

PHO85 cyclin-10

Gene

PCL10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cyclin partner of the cyclin-dependent kinase (CDK) PHO85. Together with cyclin PCL8, negatively controls glycogen accumulation under favorable growth conditions. The PCL10-PHO85 cyclin-CDK holoenzyme has glycogen synthase kinase activity and phosphorylates and negatively regulates glycogen synthase GSY2. Also has minor GLC8 kinase activity.3 Publications

GO - Molecular functioni

  • cyclin-dependent protein serine/threonine kinase regulator activity Source: SGD

GO - Biological processi

  • glycogen metabolic process Source: UniProtKB-KW
  • negative regulation of glycogen biosynthetic process Source: SGD
  • regulation of cyclin-dependent protein serine/threonine kinase activity Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Cyclin

Keywords - Biological processi

Carbohydrate metabolism, Glycogen metabolism

Enzyme and pathway databases

BioCyciYEAST:G3O-30629-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
PHO85 cyclin-10
Gene namesi
Name:PCL10
Ordered Locus Names:YGL134W
ORF Names:G2838
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL134W.
SGDiS000003102. PCL10.

Subcellular locationi

GO - Cellular componenti

  • cyclin-dependent protein kinase holoenzyme complex Source: SGD
  • cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002027401 – 433PHO85 cyclin-10Add BLAST433

Proteomic databases

MaxQBiP53124.
PRIDEiP53124.

PTM databases

iPTMnetiP53124.

Interactioni

Subunit structurei

Forms a cyclin-CDK complex with PHO85. Interacts with GSY2, independent of the presence of PHO85.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PHO85P171577EBI-23973,EBI-13327

Protein-protein interaction databases

BioGridi33118. 29 interactors.
DIPiDIP-1494N.
IntActiP53124. 13 interactors.
MINTiMINT-402996.

Structurei

Secondary structure

1433
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi246 – 248Combined sources3
Helixi262 – 280Combined sources19
Helixi291 – 298Combined sources8
Helixi310 – 321Combined sources12
Helixi325 – 339Combined sources15
Beta strandi340 – 342Combined sources3
Beta strandi348 – 350Combined sources3
Helixi356 – 358Combined sources3
Helixi359 – 374Combined sources16
Helixi381 – 388Combined sources8
Helixi392 – 406Combined sources15
Helixi415 – 429Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4KRCX-ray2.60B227-433[»]
4KRDX-ray1.95B227-433[»]
ProteinModelPortaliP53124.
SMRiP53124.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cyclin family. PHO80 subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00530000067900.
InParanoidiP53124.
OrthoDBiEOG092C2G1V.

Family and domain databases

InterProiIPR013922. Cyclin_PHO80-like.
[Graphical view]
PfamiPF08613. Cyclin. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53124-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDMTKNHTTD TEEFDDGDIR PVSLGIVDDY NASFELPLKP KFLQSENFSD
60 70 80 90 100
LTSEWDQSRS NTPGLAEGKT EKAQPCGTTD SSKNRIHVEQ LLESANEMNN
110 120 130 140 150
YLAQNIENIN NFQVGLLNGG KGLYSSMGDD SSACINGTNF SSTSNFELSD
160 170 180 190 200
DELEDTTGCT SSIFDKDLFH QQNGLSIPRR RSPLFKSPTA SFEIGDATDV
210 220 230 240 250
EEQDIDDSIF SECSSITSFD MGGLHISLPH DEEEDQEKTK SESENPLLHG
260 270 280 290 300
IPVDVEVPHI SVDEALANFK ETIELLLKLS GNRKCTGFNT RVEKKEYSNF
310 320 330 340 350
YMKSKPTLSS ADFLKRIQDK CEYQPTVYLV ATFLIDTLFL TRDGNNILQL
360 370 380 390 400
KLNLQEKEVH RMIIAAVRLS TKLLEDFVHS HEYFSKVCGI SKRLLTKLEV
410 420 430
SLLICVCNTK LMVSNRKLAA SKLLLNELRS FCV
Length:433
Mass (Da):48,584
Last modified:October 1, 1996 - v1
Checksum:iDE6608A34BDBDF99
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72656 Genomic DNA. Translation: CAA96845.1.
BK006941 Genomic DNA. Translation: DAA07976.1.
PIRiS64147.
RefSeqiNP_011381.1. NM_001180999.1.

Genome annotation databases

EnsemblFungiiYGL134W; YGL134W; YGL134W.
GeneIDi852743.
KEGGisce:YGL134W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72656 Genomic DNA. Translation: CAA96845.1.
BK006941 Genomic DNA. Translation: DAA07976.1.
PIRiS64147.
RefSeqiNP_011381.1. NM_001180999.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4KRCX-ray2.60B227-433[»]
4KRDX-ray1.95B227-433[»]
ProteinModelPortaliP53124.
SMRiP53124.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33118. 29 interactors.
DIPiDIP-1494N.
IntActiP53124. 13 interactors.
MINTiMINT-402996.

PTM databases

iPTMnetiP53124.

Proteomic databases

MaxQBiP53124.
PRIDEiP53124.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL134W; YGL134W; YGL134W.
GeneIDi852743.
KEGGisce:YGL134W.

Organism-specific databases

EuPathDBiFungiDB:YGL134W.
SGDiS000003102. PCL10.

Phylogenomic databases

GeneTreeiENSGT00530000067900.
InParanoidiP53124.
OrthoDBiEOG092C2G1V.

Enzyme and pathway databases

BioCyciYEAST:G3O-30629-MONOMER.

Miscellaneous databases

PROiP53124.

Family and domain databases

InterProiIPR013922. Cyclin_PHO80-like.
[Graphical view]
PfamiPF08613. Cyclin. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCL10_YEAST
AccessioniPrimary (citable) accession number: P53124
Secondary accession number(s): D6VU15
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 217 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.