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Protein

rRNA methyltransferase 2, mitochondrial

Gene

MRM2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent 2'-O-ribose methyltransferase that catalyzes the formation of 2'-O-methyluridine at position 2791 (Um2791) in the 21S mitochondrial large subunit ribosomal RNA (mtLSU rRNA), a universally conserved modification in the peptidyl transferase domain of the mtLSU rRNA.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + uridine(2791) in 21S rRNA = S-adenosyl-L-homocysteine + 2'-O-methyluridine(2791) in 21S rRNA.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei104 – 1041S-adenosyl-L-methionineBy similarity
Binding sitei203 – 2031S-adenosyl-L-methionineBy similarity
Active sitei264 – 2641Proton acceptorBy similarity

GO - Molecular functioni

  • rRNA (uridine-2'-O-)-methyltransferase activity Source: SGD

GO - Biological processi

  • rRNA methylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:G3O-30631-MONOMER.
BRENDAi2.1.1.168. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
rRNA methyltransferase 2, mitochondrial1 Publication (EC:2.1.1.1681 Publication)
Alternative name(s):
21S rRNA (uridine(2791)-2'-O)-methyltransferase1 Publication
21S rRNA [Um2791] 2'-O-methyltransferase1 Publication
Gene namesi
Name:MRM21 Publication
Ordered Locus Names:YGL136CImported
ORF Names:G2830
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL136C.
SGDiS000003104. MRM2.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Loses mitochondrial DNA with high frequency.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 1818MitochondrionSequence analysisAdd
BLAST
Chaini19 – 320302rRNA methyltransferase 2, mitochondrialPRO_0000155588Add
BLAST

Proteomic databases

MaxQBiP53123.

Interactioni

Protein-protein interaction databases

BioGridi33116. 51 interactions.

Structurei

3D structure databases

ProteinModelPortaliP53123.
SMRiP53123. Positions 50-121, 198-308.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni83 – 864S-adenosyl-L-methionine bindingBy similarity
Regioni178 – 1792S-adenosyl-L-methionine bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00730000111241.
HOGENOMiHOG000162366.
InParanoidiP53123.
KOiK15508.
OMAiWSQVAVN.
OrthoDBiEOG7DRJDC.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
HAMAPiMF_01547. RNA_methyltr_E.
InterProiIPR015507. rRNA-MeTfrase_E.
IPR002877. rRNA_MeTrfase_FtsJ_dom.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR10920. PTHR10920. 3 hits.
PfamiPF01728. FtsJ. 1 hit.
[Graphical view]
PIRSFiPIRSF005461. 23S_rRNA_mtase. 1 hit.
SUPFAMiSSF53335. SSF53335. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53123-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILVYNRIRS IISSSLGRIH VRYNSNSQNR WLNRQLKDPY TKEAKVQNLR
60 70 80 90 100
SRAAFKLMQI DDKYRLFSKN RTDQRILDLG YAPGAWSQVA RQRSSPNSMI
110 120 130 140 150
LGVDILPCEP PHGVNSIQAN ILAKRTHDLI RLFFSKHFQL NRHDDLHKDH
160 170 180 190 200
GYFQNMLEEE LTHVKDTELY REIFTSDDIY ETPNTNSTLI EREKFPVDVI
210 220 230 240 250
ISDMYEPWPQ TTGFWNNITN QAYFRMANTS GVSIRDHYQS IDLCDAALVT
260 270 280 290 300
AIDLLRPLGS FVCKLYTGEE ENLFKKRMQA VFTNVHKFKP DASRDESKET
310 320
YYIGLKKKRN VDKLDVFSNS
Length:320
Mass (Da):37,423
Last modified:October 1, 1996 - v1
Checksum:i2B76FC4A6628377C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92670 Genomic DNA. Translation: CAA63360.1.
Z72658 Genomic DNA. Translation: CAA96847.1.
AY558500 Genomic DNA. Translation: AAS56826.1.
BK006941 Genomic DNA. Translation: DAA07974.1.
PIRiS64149.
RefSeqiNP_011379.1. NM_001181001.1.

Genome annotation databases

EnsemblFungiiYGL136C; YGL136C; YGL136C.
GeneIDi852741.
KEGGisce:YGL136C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92670 Genomic DNA. Translation: CAA63360.1.
Z72658 Genomic DNA. Translation: CAA96847.1.
AY558500 Genomic DNA. Translation: AAS56826.1.
BK006941 Genomic DNA. Translation: DAA07974.1.
PIRiS64149.
RefSeqiNP_011379.1. NM_001181001.1.

3D structure databases

ProteinModelPortaliP53123.
SMRiP53123. Positions 50-121, 198-308.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33116. 51 interactions.

Proteomic databases

MaxQBiP53123.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL136C; YGL136C; YGL136C.
GeneIDi852741.
KEGGisce:YGL136C.

Organism-specific databases

EuPathDBiFungiDB:YGL136C.
SGDiS000003104. MRM2.

Phylogenomic databases

GeneTreeiENSGT00730000111241.
HOGENOMiHOG000162366.
InParanoidiP53123.
KOiK15508.
OMAiWSQVAVN.
OrthoDBiEOG7DRJDC.

Enzyme and pathway databases

BioCyciYEAST:G3O-30631-MONOMER.
BRENDAi2.1.1.168. 984.

Miscellaneous databases

NextBioi972156.
PROiP53123.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
HAMAPiMF_01547. RNA_methyltr_E.
InterProiIPR015507. rRNA-MeTfrase_E.
IPR002877. rRNA_MeTrfase_FtsJ_dom.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR10920. PTHR10920. 3 hits.
PfamiPF01728. FtsJ. 1 hit.
[Graphical view]
PIRSFiPIRSF005461. 23S_rRNA_mtase. 1 hit.
SUPFAMiSSF53335. SSF53335. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of a 14.6 kb DNA fragment of Saccharomyces cerevisiae chromosome VII reveals SEC27, SSM1b, a putative S-adenosylmethionine-dependent enzyme and six new open reading frames."
    Escribano V., Eraso P., Portillo F., Mazon M.J.
    Yeast 12:887-892(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "MRM2 encodes a novel yeast mitochondrial 21S rRNA methyltransferase."
    Pintard L., Bujnicki J.M., Lapeyre B., Bonnerot C.
    EMBO J. 21:1139-1147(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiMRM2_YEAST
AccessioniPrimary (citable) accession number: P53123
Secondary accession number(s): D6VU13
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 11, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.