Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

rRNA methyltransferase 2, mitochondrial

Gene

MRM2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent 2'-O-ribose methyltransferase that catalyzes the formation of 2'-O-methyluridine at position 2791 (Um2791) in the 21S mitochondrial large subunit ribosomal RNA (mtLSU rRNA), a universally conserved modification in the peptidyl transferase domain of the mtLSU rRNA.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + uridine(2791) in 21S rRNA = S-adenosyl-L-homocysteine + 2'-O-methyluridine(2791) in 21S rRNA.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei104S-adenosyl-L-methionineBy similarity1
Binding sitei203S-adenosyl-L-methionineBy similarity1
Active sitei264Proton acceptorBy similarity1

GO - Molecular functioni

  • rRNA (uridine-2'-O-)-methyltransferase activity Source: SGD

GO - Biological processi

  • rRNA methylation Source: SGD

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processrRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:G3O-30631-MONOMER
BRENDAi2.1.1.168 984

Names & Taxonomyi

Protein namesi
Recommended name:
rRNA methyltransferase 2, mitochondrial1 Publication (EC:2.1.1.1681 Publication)
Alternative name(s):
21S rRNA (uridine(2791)-2'-O)-methyltransferase1 Publication
21S rRNA [Um2791] 2'-O-methyltransferase1 Publication
Gene namesi
Name:MRM21 Publication
Ordered Locus Names:YGL136CImported
ORF Names:G2830
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL136C
SGDiS000003104 MRM2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Loses mitochondrial DNA with high frequency.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 18MitochondrionSequence analysisAdd BLAST18
ChainiPRO_000015558819 – 320rRNA methyltransferase 2, mitochondrialAdd BLAST302

Proteomic databases

MaxQBiP53123
PaxDbiP53123
PRIDEiP53123

Interactioni

Protein-protein interaction databases

BioGridi33116, 558 interactors
IntActiP53123, 2 interactors
STRINGi4932.YGL136C

Structurei

3D structure databases

ProteinModelPortaliP53123
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni83 – 86S-adenosyl-L-methionine bindingBy similarity4
Regioni178 – 179S-adenosyl-L-methionine bindingBy similarity2

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00730000111241
HOGENOMiHOG000162366
InParanoidiP53123
KOiK15508
OMAiMSAPWDQ
OrthoDBiEOG092C3GU7

Family and domain databases

HAMAPiMF_01547 RNA_methyltr_E, 1 hit
InterProiView protein in InterPro
IPR015507 rRNA-MeTfrase_E
IPR002877 rRNA_MeTrfase_FtsJ_dom
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF01728 FtsJ, 1 hit
PIRSFiPIRSF005461 23S_rRNA_mtase, 1 hit
SUPFAMiSSF53335 SSF53335, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53123-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILVYNRIRS IISSSLGRIH VRYNSNSQNR WLNRQLKDPY TKEAKVQNLR
60 70 80 90 100
SRAAFKLMQI DDKYRLFSKN RTDQRILDLG YAPGAWSQVA RQRSSPNSMI
110 120 130 140 150
LGVDILPCEP PHGVNSIQAN ILAKRTHDLI RLFFSKHFQL NRHDDLHKDH
160 170 180 190 200
GYFQNMLEEE LTHVKDTELY REIFTSDDIY ETPNTNSTLI EREKFPVDVI
210 220 230 240 250
ISDMYEPWPQ TTGFWNNITN QAYFRMANTS GVSIRDHYQS IDLCDAALVT
260 270 280 290 300
AIDLLRPLGS FVCKLYTGEE ENLFKKRMQA VFTNVHKFKP DASRDESKET
310 320
YYIGLKKKRN VDKLDVFSNS
Length:320
Mass (Da):37,423
Last modified:October 1, 1996 - v1
Checksum:i2B76FC4A6628377C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92670 Genomic DNA Translation: CAA63360.1
Z72658 Genomic DNA Translation: CAA96847.1
AY558500 Genomic DNA Translation: AAS56826.1
BK006941 Genomic DNA Translation: DAA07974.1
PIRiS64149
RefSeqiNP_011379.1, NM_001181001.1

Genome annotation databases

EnsemblFungiiYGL136C; YGL136C; YGL136C
GeneIDi852741
KEGGisce:YGL136C

Similar proteinsi

Entry informationi

Entry nameiMRM2_YEAST
AccessioniPrimary (citable) accession number: P53123
Secondary accession number(s): D6VU13
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health