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Protein

Probable E3 ubiquitin-protein ligase HUL5

Gene

HUL5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probable E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Non essential.

Miscellaneous

A cysteine residue is required for ubiquitin-thioester formation.By similarity
Present with 3120 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei878Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • ubiquitin-ubiquitin ligase activity Source: SGD

GO - Biological processi

  • free ubiquitin chain polymerization Source: SGD
  • protein polyubiquitination Source: SGD
  • protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: SGD
  • ubiquitin-dependent ERAD pathway Source: SGD

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-30636-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable E3 ubiquitin-protein ligase HUL5 (EC:2.3.2.26)
Alternative name(s):
HECT-type E3 ubiquitin transferase HUL5
Gene namesi
Name:HUL5
Ordered Locus Names:YGL141W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL141W.
SGDiS000003109. HUL5.

Subcellular locationi

GO - Cellular componenti

  • proteasome complex Source: SGD

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000840951 – 910Probable E3 ubiquitin-protein ligase HUL5Add BLAST910

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP53119.
PRIDEiP53119.

PTM databases

iPTMnetiP53119.

Interactioni

Protein-protein interaction databases

BioGridi33111. 71 interactors.
DIPiDIP-6349N.
IntActiP53119. 2 interactors.
MINTiMINT-624731.
STRINGi4932.YGL141W.

Structurei

3D structure databases

ProteinModelPortaliP53119.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini810 – 910HECTPROSITE-ProRule annotationAdd BLAST101

Phylogenomic databases

GeneTreeiENSGT00550000074668.
HOGENOMiHOG000074709.
InParanoidiP53119.
KOiK10589.
OMAiNYELYPS.
OrthoDBiEOG092C0WAY.

Family and domain databases

CDDicd00078. HECTc. 1 hit.
InterProiView protein in InterPro
IPR000569. HECT_dom.
PfamiView protein in Pfam
PF00632. HECT. 1 hit.
SMARTiView protein in SMART
SM00119. HECTc. 1 hit.
SUPFAMiSSF56204. SSF56204. 1 hit.
PROSITEiView protein in PROSITE
PS50237. HECT. 1 hit.

Sequencei

Sequence statusi: Complete.

P53119-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNFTGQTRR RNVNLGNRTR NSKKDLLEKA KRERERRAQD KLKEDASKTI
60 70 80 90 100
QKSIRRHFSN VRLFKNTFTS SQLVHMIPAY GGKLIYYISQ YDLQQLLKLS
110 120 130 140 150
HNFLSSYPNS LGNRQLLSLL KLYQDDALVA ETLSDLNMDC PTVDEFLDSL
160 170 180 190 200
SVYLCRASFL SYSSASKLAD VIEAWEVMHS SASISIFSIS IGSYEKRPFA
210 220 230 240 250
LQFYCILAER NLLPQLINTN PILWDNMAKT YSHCSKGGQK NIAKLLIPNF
260 270 280 290 300
NNHIAPSVLR SDNDYVLKFY EKAFIDEVIA TTANYVSDED HVKNLMCYIA
310 320 330 340 350
SSPNQSCKNS VLITLLSNKD FVRRLSWEFF HTKFNASKTE AHPLFSVLAQ
360 370 380 390 400
LIDMHLLIST DRELLDYNSV IPIEELKKFT STLKDFTFRQ YWELPKSERN
410 420 430 440 450
PMLKEAVPLL SKVYERDSRL HFLSTENNPT YWENSEKQFL NLRFYEELQE
460 470 480 490 500
YEDLYREHLE EESDEDMEKE IDLDKERPPL KSLLLNKMKK RLKSSLRFRK
510 520 530 540 550
LEILLELPFF IPFEERVDLF YMFIALDKKR LSLDDDHNLI NMFTPWASTG
560 570 580 590 600
MRKQSAIISR DNVLEDAFNA FNSIGERFKA SLDVTFINEF GEEAGIDGGG
610 620 630 640 650
ITKEFLTTVS DEGFKDPKHE LFRTNDRYEL YPSVVYDATK LKYIWFLGKV
660 670 680 690 700
VGKCLYEHVL IDVSFADFFL KKLLNYSNGF LSSFSDLGSY DSVLYNNLIK
710 720 730 740 750
LLNMTTDEIK SLDLTFEIDE PESSAKVVDL IPNGSKTYVT KDNVLLYVTK
760 770 780 790 800
VTDYKLNKRC FKPVSAFHGG LSVIIAPHWM EMFNSIELQM LISGERDNID
810 820 830 840 850
LDDLKSNTEY GGYKEEDQTI VDFWEVLNEF KFEEKLNFLK FVTSVPQAPL
860 870 880 890 900
QGFKALDPKF GIRNAGTEKY RLPTASTCVN LLKLPDYRNK TILREKLLYA
910
INSGARFDLS
Length:910
Mass (Da):105,566
Last modified:October 1, 1996 - v1
Checksum:i8DB832A48A81CD19
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99960 Genomic DNA. Translation: CAA68221.1.
Z72663 Genomic DNA. Translation: CAA96853.1.
AY723811 Genomic DNA. Translation: AAU09728.1.
BK006941 Genomic DNA. Translation: DAA07969.1.
PIRiS64155.
RefSeqiNP_011374.1. NM_001181006.1.

Genome annotation databases

EnsemblFungiiYGL141W; YGL141W; YGL141W.
GeneIDi852736.
KEGGisce:YGL141W.

Similar proteinsi

Entry informationi

Entry nameiHUL5_YEAST
AccessioniPrimary (citable) accession number: P53119
Secondary accession number(s): D6VU08
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 5, 2017
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names