P53115 (INO80_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative DNA helicase INO80
EC=3.6.4.12

Alternative name(s):
Inositol-requiring protein 80

Gene names

Name:	INO80
Ordered Locus Names:	YGL150C
ORF Names:	G1880

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1489 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Putative DNA helicase component of the INO80 complex which remodels chromatin by shifting nucleosomes. Its ability to induce transcription of some phosphate-responsive genes is modulated by inositol polyphosphates. The INO80 complex is involved in DNA repair by associating to 'Ser-129' phosphorylated H2A histones as a response to DNA damage. Ref.5 Ref.6 Ref.8 Ref.12 Ref.13
Catalytic activity	ATP + H2O = ADP + phosphate.
Subunit structure	Component of the chromatin-remodeling INO80 complex, at least composed of ARP4, ARP5, ARP8, RVB1, RVB2, TAF14, NHP10, IES1, IES3, IES4, IES6, ACT1, IES2, IES5 and INO80.
Subcellular location	Nucleus Ref.9.
Miscellaneous	Present with 1110 molecules/cell in log phase SD medium. Ref.10
Sequence similarities	Belongs to the SNF2/RAD54 helicase family. Contains 1 DBINO domain. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Biological process	DNA damage DNA repair Transcription Transcription regulation
Cellular component	Nucleus
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Activator Chromatin regulator Helicase Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from direct assay Ref.5. Source: SGD chromatin silencing at telomere Inferred from mutant phenotype. Source: SGD histone exchange Inferred from direct assay. Source: SGD nucleosome mobilization Inferred from mutant phenotype. Source: SGD nucleosome positioning Inferred from direct assay. Source: SGD positive regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype. Source: SGD regulation of transcription from RNA polymerase II promoter in response to stress Inferred from mutant phenotype. Source: SGD transcription from RNA polymerase II promoter Inferred from direct assay Ref.5. Source: SGD
Cellular component	Ino80 complex Inferred from direct assay Ref.6. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity Inferred from direct assay Ref.5. Source: SGD DNA binding Inferred from electronic annotation. Source: UniProtKB-KW helicase activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NHP10	Q03435	6	EBI-24019,EBI-12010

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1489	1489	Putative DNA helicase INO80		PRO_0000074329
Regions
	Domain	476 – 601	126	DBINO
	Domain	718 – 890	173	Helicase ATP-binding
	Domain	1303 – 1467	165	Helicase C-terminal
	Nucleotide binding	731 – 738	8	ATP Potential
	Motif	841 – 844	4	DEAQ box
	Compositional bias	188 – 193	6	Poly-Ala
	Compositional bias	259 – 268	10	Poly-Glu
	Compositional bias	300 – 306	7	Poly-Ser
	Compositional bias	568 – 573	6	Poly-Glu
	Compositional bias	675 – 682	8	Poly-Glu
Amino acid modifications
	Modified residue	65	1	Phosphoserine Ref.14 Ref.15 Ref.16
	Modified residue	115	1	Phosphoserine Ref.15 Ref.16
	Modified residue	133	1	Phosphoserine Ref.16
	Modified residue	1469	1	Phosphothreonine Ref.16
	Modified residue	1477	1	Phosphoserine Ref.16
Experimental info
	Mutagenesis	737	1	K → A: Reduced ATPase activity of the INO80 complex. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P53115 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 017053C5A245337E	FASTA	1,489	171,455
	10 20 30 40 50 60 MSLAVLLNKE DKDISDFSKT TAGKSAKKNS RERVADVAPT RVLDKKQAYL SQLNSEFNRI 70 80 90 100 110 120 KRRDSIEQLY QDWKFINLQE FELISEWNQQ SKDWQFDNTN DSQDLHFKKL YRDMSMINKE 130 140 150 160 170 180 WAEYQSFKNA NLSDIINEKD ADEDEEDDED ELEDGEEDME EDEASTGRHT NGKSMRGNGI 190 200 210 220 230 240 QKSRKKDAAA AAAIGKAIKD DQTHADTVVT VNGDENEDGN NGEDEDNDND NENNNDNDND 250 260 270 280 290 300 NENENDNDSD NDDEEENGEE DEEEEEIEDL DEEDFAAFEE QDDNDDEDFN PDVEKRRKRS 310 320 330 340 350 360 SSSSSSTKLS MNSLSLITSK KINKNITINS DRPKIVRELI KMCNKNKHQK IKKRRFTNCI 370 380 390 400 410 420 VTDYNPIDSK LNIKITLKQY HVKRLKKLIN DAKREREREE ALKNNVGLDG NDLDNDEDGS 430 440 450 460 470 480 ESHKRRKLNN NTANGADDAN KRKFNTRHGL PTYGMKMNAK EARAIQRHYD NTYTTIWKDM 490 500 510 520 530 540 ARKDSTKMSR LVQQIQSIRS TNFRKTSSLC AREAKKWQSK NFKQIKDFQT RARRGIREMS 550 560 570 580 590 600 NFWKKNEREE RDLKKKIEKE AMEQAKKEEE EKESKRQAKK LNFLLTQTEL YSHFIGRKIK 610 620 630 640 650 660 TNELEGNNVS SNDSESQKNI DISALAPNKN DFHAIDFDNE NDEQLRLRAA ENASNALAET 670 680 690 700 710 720 RAKAKQFDDH ANAHEEEEEE DELNFQNPTS LGEITIEQPK ILACTLKEYQ LKGLNWLANL 730 740 750 760 770 780 YDQGINGILA DEMGLGKTVQ SISVLAHLAE NHNIWGPFLV VTPASTLHNW VNEISKFLPQ 790 800 810 820 830 840 FKILPYWGNA NDRKVLRKFW DRKNLRYNKN APFHVMVTSY QMVVTDANYL QKMKWQYMIL 850 860 870 880 890 900 DEAQAIKSSQ SSRWKNLLSF HCRNRLLLTG TPIQNSMQEL WALLHFIMPS LFDSHDEFNE 910 920 930 940 950 960 WFSKDIESHA EANTKLNQQQ LRRLHMILKP FMLRRVKKNV QSELGDKIEI DVLCDLTQRQ 970 980 990 1000 1010 1020 AKLYQVLKSQ ISTNYDAIEN AATNDSTSNS ASNSGSDQNL INAVMQFRKV CNHPDLFERA 1030 1040 1050 1060 1070 1080 DVDSPFSFTT FGKTTSMLTA SVANNNSSVI SNSNMNLSSM SSNNISNGKF TDLIYSSRNP 1090 1100 1110 1120 1130 1140 IKYSLPRLIY EDLILPNYNN DVDIANKLKN VKFNIFNPST NYELCLFLSK LTGEPSLNEF 1150 1160 1170 1180 1190 1200 FRVSTTPLLK RVIERTNGPK NTDSLSFKTI TQELLEVTRN APSEGVMASL LNVEKHAYER 1210 1220 1230 1240 1250 1260 EYLNCIQRGY HPNVSAPPVT IEVLGSSHVT NSINNELFDP LISQALSDIP AITQYNMHVK 1270 1280 1290 1300 1310 1320 KGIPVEDFPK TGLFPEPLNK NFSSNISMPS MDRFITESAK LRKLDELLVK LKSEGHRVLI 1330 1340 1350 1360 1370 1380 YFQMTKMMDL MEEYLTYRQY NHIRLDGSSK LEDRRDLVHD WQTNPEIFVF LLSTRAGGLG 1390 1400 1410 1420 1430 1440 INLTAADTVI FYDSDWNPTI DSQAMDRAHR LGQTRQVTVY RLLVRGTIEE RMRDRAKQKE 1450 1460 1470 1480 QVQQVVMEGK TQEKNIKTIE VGENDSEVTR EGSKSISQDG IKEAASALA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence analysis of a 35 kb segment from Saccharomyces cerevisiae chromosome VII reveals 19 open reading frames including RAD54, ACE1/CUP2, PMR1, RCK1, AMS1 and CAL1/CDC43." James C.M., Indge K.J., Oliver S.G. Yeast 11:1413-1419(1995) [PubMed: 8585324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"The sequence of a nearly unclonable 22.8 kb segment on the left arm chromosome VII from Saccharomyces cerevisiae reveals ARO2, RPL9A, TIP1, MRF1 genes and six new open reading frames." Voet M., Defoor E., Verhasselt P., Riles L., Robben J., Volckaert G. Yeast 13:177-182(1997) [PubMed: 9046099] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	"A chromatin remodelling complex involved in transcription and DNA processing." Shen X., Mizuguchi G., Hamiche A., Wu C. Nature 406:541-544(2000) [PubMed: 10952318] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, IDENTIFICATION IN THE INO80 COMPLEX, FUNCTION, MUTAGENESIS OF LYS-737.
[6]	"The product of the SNF2/SWI2 paralogue INO80 of Saccharomyces cerevisiae required for efficient expression of various yeast structural genes is part of a high-molecular-weight protein complex." Ebbert R., Birkmann A., Schueller H.-J. Mol. Microbiol. 32:741-751(1999) [PubMed: 10361278] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN A HIGH MOLECULAR WEIGHT COMPLEX.
[7]	"Systematic identification, classification, and characterization of the open reading frames which encode novel helicase-related proteins in Saccharomyces cerevisiae by gene disruption and Northern analysis." Shiratori A., Shibata T., Arisawa M., Hanaoka F., Murakami Y., Eki T. Yeast 15:219-253(1999) [PubMed: 10077188] [Abstract] Cited for: IDENTIFICATION AS A PUTATIVE HELICASE.
[8]	"Involvement of actin-related proteins in ATP-dependent chromatin remodeling." Shen X., Ranallo R., Choi E., Wu C. Mol. Cell 12:147-155(2003) [PubMed: 12887900] [Abstract] Cited for: FUNCTION OF THE INO80 COMPLEX.
[9]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]	"Regulation of chromatin remodeling by inositol polyphosphates." Steger D.J., Haswell E.S., Miller A.L., Wente S.R., O'Shea E.K. Science 299:114-116(2003) [PubMed: 12434012] [Abstract] Cited for: REGULATION OF THE INO80 COMPLEX BY INOSITOL POLYPHOSPHATES.
[12]	"INO80 and gamma-H2AX interaction links ATP-dependent chromatin remodeling to DNA damage repair." Morrison A.J., Highland J., Krogan N.J., Arbel-Eden A., Greenblatt J.F., Haber J.E., Shen X. Cell 119:767-775(2004) [PubMed: 15607974] [Abstract] Cited for: FUNCTION OF THE INO80 COMPLEX.
[13]	"Recruitment of the INO80 complex by H2A phosphorylation links ATP-dependent chromatin remodeling with DNA double-strand break repair." van Attikum H., Fritsch O., Hohn B., Gasser S.M. Cell 119:777-788(2004) [PubMed: 15607975] [Abstract] Cited for: FUNCTION OF THE INO80 COMPLEX.
[14]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65, MASS SPECTROMETRY. Strain: ADR376.
[15]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65 AND SER-115, MASS SPECTROMETRY.
[16]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65; SER-115; SER-133; THR-1469 AND SER-1477, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z48618 Genomic DNA. Translation: CAA88537.1. Z72672 Genomic DNA. Translation: CAA96861.1. X99960 Genomic DNA. Translation: CAA68224.1. BK006941 Genomic DNA. Translation: DAA07961.1.
PIR	S60416.
RefSeq	NP_011365.1. NM_001181015.1.
3D structure databases
ProteinModelPortal	P53115.
SMR	P53115. Positions 696-1450.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1386N.
IntAct	P53115. 46 interactions.
MINT	MINT-388693.
STRING	P53115.
Proteomic databases
PeptideAtlas	P53115.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL150C; YGL150C; YGL150C.
GeneID	852728.
KEGG	sce:YGL150C.
NMPDR	fig|4932.3.peg.2469.
Organism-specific databases
CYGD	YGL150c.
SGD	S000003118. INO80.
Phylogenomic databases
eggNOG	fuNOG06280.
GeneTree	EFGT00050000000148.
HOGENOM	HBG398013.
OMA	SHDEFSD.
OrthoDB	EOG41G6C8.
Gene expression databases
ArrayExpress	P53115.
Genevestigator	P53115.
GermOnline	YGL150C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR014001. DEAD-like_helicase. IPR020838. DNA_binding_INO80. IPR001650. Helicase_C. IPR000330. SNF2_N. [Graphical view]
KO	K11665.
Pfam	PF00271. Helicase_C. 1 hit. PF00176. SNF2_N. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS51413. DBINO. 1 hit. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	972117.

Entry information

Entry name

INO80_YEAST

Accession

Primary (citable) accession number: P53115
Secondary accession number(s): D6VU00

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program