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P53111 (ARI1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADPH-dependent aldehyde reductase ARI1

EC=1.1.1.-
Gene names
Name:ARI1
Ordered Locus Names:YGL157W
ORF Names:G1857
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length347 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

NADPH-dependent aldehyde reductase involved in the detoxification of aldehyde inhibitors derived from lignocellulosic biomass conversion. Reduces commonly detected inhibitors in biomass conversion hydrolysates such as furfural, 5-hydroxymethylfurfural (HMF), cinnamic aldehyde, benzaldehyde, phenylacetaldehyde, and anisaldehyde. Ref.4 Ref.7 Ref.8

Cofactor

NADPH. Ref.7 Ref.8

Subcellular location

Cytoplasm. Nucleus Ref.5.

Induction

By furfural and 5-hydroxymethylfurfural (HMF). Ref.7

Sequence similarities

Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.

Biophysicochemical properties

pH dependence:

Optimum pH is 7.0. Ref.7

Temperature dependence:

Optimum temperature is 25 degrees Celsius.

Ontologies

Keywords
   Cellular componentCytoplasm
Nucleus
   LigandNADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncarbonyl reductase (NADPH) activity

Inferred from direct assay PubMed 19016485Ref.7. Source: SGD

coenzyme binding

Inferred from electronic annotation. Source: InterPro

oxidoreductase activity

Inferred from direct assay Ref.4. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 347347NADPH-dependent aldehyde reductase ARI1
PRO_0000215578

Sequences

Sequence LengthMass (Da)Tools
P53111 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 51035AD526E418AD

FASTA34738,083
        10         20         30         40         50         60 
MTTDTTVFVS GATGFIALHI MNDLLKAGYT VIGSGRSQEK NDGLLKKFNN NPKLSMEIVE 

        70         80         90        100        110        120 
DIAAPNAFDE VFKKHGKEIK IVLHTASPFH FETTNFEKDL LTPAVNGTKS ILEAIKKYAA 

       130        140        150        160        170        180 
DTVEKVIVTS STAALVTPTD MNKGDLVITE ESWNKDTWDS CQANAVAAYC GSKKFAEKTA 

       190        200        210        220        230        240 
WEFLKENKSS VKFTLSTINP GFVFGPQMFA DSLKHGINTS SGIVSELIHS KVGGEFYNYC 

       250        260        270        280        290        300 
GPFIDVRDVS KAHLVAIEKP ECTGQRLVLS EGLFCCQEIV DILNEEFPQL KGKIATGEPA 

       310        320        330        340 
TGPSFLEKNS CKFDNSKTKK LLGFQFYNLK DCIVDTAAQM LEVQNEA 

« Hide

References

« Hide 'large scale' references
[1]"DNA sequence analysis of a 35 kb segment from Saccharomyces cerevisiae chromosome VII reveals 19 open reading frames including RAD54, ACE1/CUP2, PMR1, RCK1, AMS1 and CAL1/CDC43."
James C.M., Indge K.J., Oliver S.G.
Yeast 11:1413-1419(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Efficient anaerobic whole cell stereoselective bioreduction with recombinant Saccharomyces cerevisiae."
Katz M., Frejd T., Hahn-Hagerdal B., Gorwa-Grauslund M.F.
Biotechnol. Bioeng. 84:573-582(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"A novel NADPH-dependent aldehyde reductase gene from Saccharomyces cerevisiae NRRL Y-12632 involved in the detoxification of aldehyde inhibitors derived from lignocellulosic biomass conversion."
Liu Z.L., Moon J.
Gene 446:1-10(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION, FUNCTION, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES.
[8]"Stereochemistry of furfural reduction by a Saccharomyces cerevisiae aldehyde reductase that contributes to in situ furfural detoxification."
Bowman M.J., Jordan D.B., Vermillion K.E., Braker J.D., Moon J., Liu Z.L.
Appl. Environ. Microbiol. 76:4926-4932(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, COFACTOR.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z48618 Genomic DNA. No translation available.
Z72679 Genomic DNA. Translation: CAA96869.1.
BK006941 Genomic DNA. Translation: DAA07955.1.
PIRS60428.
RefSeqNP_011358.3. NM_001181022.3.

3D structure databases

ProteinModelPortalP53111.
SMRP53111. Positions 6-331.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33097. 13 interactions.
DIPDIP-5112N.
IntActP53111. 8 interactions.
MINTMINT-513533.
STRING4932.YGL157W.

Proteomic databases

PaxDbP53111.
PeptideAtlasP53111.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGL157W; YGL157W; YGL157W.
GeneID852720.
KEGGsce:YGL157W.

Organism-specific databases

CYGDYGL157w.
SGDS000003125. ARI1.

Phylogenomic databases

eggNOGCOG0451.
GeneTreeENSGT00390000002618.
OMAFINDETT.
OrthoDBEOG77M8ZW.

Enzyme and pathway databases

BioCycYEAST:G3O-30646-MONOMER.

Gene expression databases

GenevestigatorP53111.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR001509. Epimerase_deHydtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF01370. Epimerase. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio972099.

Entry information

Entry nameARI1_YEAST
AccessionPrimary (citable) accession number: P53111
Secondary accession number(s): D6VTZ4
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families