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P53104

- ATG1_YEAST

UniProt

P53104 - ATG1_YEAST

Protein

Serine/threonine-protein kinase ATG1

Gene

ATG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 141 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Serine/threonine protein kinase involved in the cytoplasm to vacuole transport (Cvt) and found to be essential in autophagy, where it is required for the formation of autophagosomes. Involved in the clearance of protein aggregates which cannot be efficiently cleared by the proteasome. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Also involved in endoplasmic reticulum-specific autophagic process, in selective removal of ER-associated degradation (ERAD) substrates. Plays a key role in ATG9 and ATG23 cycling through the pre-autophagosomal structure and is necessary to promote ATG18 binding to ATG9. Finally, ATG1 is also required for the maintenance of cell viability under starvation and for glycogen storage during stationary phase. Plays a role in genome stability through suppression of abnormal mitosis under starvation, and in regulation of filamentous growth.32 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Activated by hypophosphorylated form of ATG13 (present in nitrogen starvation conditions). Also activated by autophopsphorylation of Thr-226 and inhibited by phosphorylation of Ser-34.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei54 – 541ATPPROSITE-ProRule annotation
    Active sitei172 – 1721Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi30 – 389ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. protein serine/threonine kinase activity Source: SGD

    GO - Biological processi

    1. autophagic vacuole assembly Source: SGD
    2. autophagy Source: SGD
    3. CVT pathway Source: SGD
    4. late nucleophagy Source: SGD
    5. mitochondrion degradation Source: SGD
    6. piecemeal microautophagy of nucleus Source: SGD
    7. protein autophosphorylation Source: SGD
    8. protein phosphorylation Source: SGD

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Autophagy, Protein transport, Transport

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30667-MONOMER.
    BRENDAi2.7.11.1. 984.

    Protein family/group databases

    TCDBi9.A.15.1.1. the autophagy-related phagophore-formation transporter (apt) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase ATG1 (EC:2.7.11.1)
    Alternative name(s):
    Autophagy protein 3
    Autophagy-related protein 1
    Cytoplasm to vacuole targeting protein 10
    Gene namesi
    Name:ATG1
    Synonyms:APG1, AUT3, CVT10
    Ordered Locus Names:YGL180W
    ORF Names:G1615
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGL180w.
    SGDiS000003148. ATG1.

    Subcellular locationi

    Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein
    Note: Formes punctate structures in starvation conditions only when ATG13 and ATG17 were both present. Localizes to both the isolation membrane (IM) and the vacuole-isolation membrane contact site (VICS) during IM expansion. The IM is a membrane sac generated from the pre-autophagosomal structure that ultimately expands to become a mature autophagosome.

    GO - Cellular componenti

    1. ATG1/UKL1 signaling complex Source: SGD
    2. autophagic vacuole membrane Source: SGD
    3. cytosol Source: SGD
    4. pre-autophagosomal structure Source: SGD
    5. pre-autophagosomal structure membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi34 – 341S → D or E: Impairs kinase activity. 1 Publication
    Mutagenesisi54 – 541K → A: Defect in the Cvt pathway. 3 Publications
    Mutagenesisi102 – 1021M → A: Enables the binding of the inhibitor of the kinase activity 1-NA-PP1. 1 Publication
    Mutagenesisi211 – 2111D → A: Loss of cell viability under starvation. 2 Publications
    Mutagenesisi226 – 2261T → E: Leads to constitutive autophosphorylation activity. 1 Publication
    Mutagenesisi237 – 2371E → R: Loss of cell viability under starvation. 1 Publication
    Mutagenesisi429 – 4291Y → A: Impairs interaction with ATG8 and decreases autophagy efficiency; when associated with A-432. 1 Publication
    Mutagenesisi432 – 4321V → A: Impairs interaction with ATG8 and decreases autophagy efficiency; when associated with A-429. 1 Publication
    Mutagenesisi508 – 5081S → A: Impairs autophagic activity; when associated with A-515. 1 Publication
    Mutagenesisi515 – 5151S → A: Impairs autophagic activity; when associated with A-508. 1 Publication
    Mutagenesisi551 – 5511S → A: Decreases autophagic activity. 1 Publication
    Mutagenesisi552 – 5521S → A: Decreases autophagic activity. 1 Publication
    Mutagenesisi621 – 6211S → A: Decreases autophagic activity. 1 Publication
    Mutagenesisi677 – 6771S → A: Decreases autophagic activity. 1 Publication
    Mutagenesisi680 – 6801S → A: Decreases autophagic activity. 1 Publication
    Mutagenesisi683 – 6831S → A: Decreases autophagic activity. 1 Publication
    Mutagenesisi769 – 7691S → A: Decreases autophagic activity. 1 Publication
    Mutagenesisi783 – 7831S → A: Decreases autophagic activity. 1 Publication
    Mutagenesisi884 – 8841N → A: No effect. 1 Publication
    Mutagenesisi886 – 8861L → G: Cvt pathway specific block. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 897897Serine/threonine-protein kinase ATG1PRO_0000085655Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei34 – 341Phosphoserine2 Publications
    Modified residuei129 – 1291Phosphothreonine2 Publications
    Modified residuei226 – 2261Phosphothreonine; by autocatalysis2 Publications
    Modified residuei304 – 3041Phosphoserine2 Publications
    Modified residuei365 – 3651Phosphoserine2 Publications
    Modified residuei390 – 3901Phosphoserine3 Publications
    Modified residuei508 – 5081Phosphoserine; by PKA2 Publications
    Modified residuei515 – 5151Phosphoserine; by PKA3 Publications
    Modified residuei533 – 5331Phosphoserine2 Publications
    Modified residuei551 – 5511Phosphoserine2 Publications
    Modified residuei552 – 5521Phosphoserine2 Publications
    Modified residuei590 – 5901Phosphothreonine2 Publications
    Modified residuei621 – 6211Phosphoserine2 Publications
    Modified residuei635 – 6351Phosphoserine2 Publications
    Modified residuei638 – 6381Phosphoserine2 Publications
    Modified residuei647 – 6471Phosphoserine2 Publications
    Modified residuei677 – 6771Phosphoserine3 Publications
    Modified residuei680 – 6801Phosphoserine2 Publications
    Modified residuei683 – 6831Phosphoserine2 Publications
    Modified residuei769 – 7691Phosphoserine2 Publications
    Modified residuei783 – 7831Phosphoserine2 Publications

    Post-translational modificationi

    Autophosphorylated at Thr-226 and Ser-390. The phosphorylation state may play a role in the induction of protein degradation upon starvation. Phosphorylation at Thr-226 within the activation loop is required for protein kinase activity whereas phosphorylation at Ser-34 leads to inhibition of kinase activity. Phosphorylation of Ser-508 and Ser-515 by PKA is required to induce autophagy but not for kinase activity.7 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP53104.
    PaxDbiP53104.
    PRIDEiP53104.

    Expressioni

    Gene expression databases

    GenevestigatoriP53104.

    Interactioni

    Subunit structurei

    Homodimer. Dimerization requires the presence of ATG13. Forms a ternary complex with ATG13 and ATG17. Interacts also with ATG11.6 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ATG11Q125273EBI-2657,EBI-31977
    ATG13Q0662813EBI-2657,EBI-36188
    ATG8P381823EBI-2657,EBI-2684

    Protein-protein interaction databases

    BioGridi33074. 374 interactions.
    DIPiDIP-1192N.
    IntActiP53104. 21 interactions.
    MINTiMINT-405956.
    STRINGi4932.YGL180W.

    Structurei

    3D structure databases

    ProteinModelPortaliP53104.
    SMRiP53104. Positions 17-358.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini24 – 325302Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni880 – 8867Required for Cvt trafficking

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi429 – 4324LIR

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi542 – 5487Poly-Gln

    Domaini

    The LIR motif is required for the interaction with ATG8 and for the association of ATG1 with autophagosomes.2 Publications

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00750000117559.
    HOGENOMiHOG000246715.
    KOiK08269.
    OMAiEKLMYDR.
    OrthoDBiEOG7CCC0Z.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    IPR022708. Ser/Thr_kinase_C.
    [Graphical view]
    PfamiPF12063. DUF3543. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53104-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGDIKNKDHT TSVNHNLMAS AGNYTAEKEI GKGSFATVYR GHLTSDKSQH    50
    VAIKEVSRAK LKNKKLLENL EIEIAILKKI KHPHIVGLID CERTSTDFYL 100
    IMEYCALGDL TFLLKRRKEL MENHPLLRTV FEKYPPPSEN HNGLHRAFVL 150
    SYLQQLASAL KFLRSKNLVH RDIKPQNLLL STPLIGYHDS KSFHELGFVG 200
    IYNLPILKIA DFGFARFLPN TSLAETLCGS PLYMAPEILN YQKYNAKADL 250
    WSVGTVVFEM CCGTPPFRAS NHLELFKKIK RANDVITFPS YCNIEPELKE 300
    LICSLLTFDP AQRIGFEEFF ANKVVNEDLS SYELEDDLPE LESKSKGIVE 350
    SNMFVSEYLS KQPKSPNSNL AGHQSMADNP AELSDALKNS NILTAPAVKT 400
    DHTQAVDKKA SNNKYHNSLV SDRSFEREYV VVEKKSVEVN SLADEVAQAG 450
    FNPNPIKHPT STQNQNVLLN EQFSPNNQQY FQNQGENPRL LRATSSSSGG 500
    SDGSRRPSLV DRRLSISSLN PSNALSRALG IASTRLFGGA NQQQQQQQIT 550
    SSPPYSQTLL NSQLFHELTE NIILRIDHLQ HPETLKLDNT NIVSILESLA 600
    AKAFVVYSYA EVKFSQIVPL STTLKGMANF ENRRSMDSNA IAEEQDSDDA 650
    EEEDETLKKY KEDCLSTKTF GKGRTLSATS QLSATFNKLP RSEMILLCNE 700
    AIVLYMKALS ILSKSMQVTS NWWYESQEKS CSLRVNVLVQ WLREKFNECL 750
    EKADFLRLKI NDLRFKHASE VAENQTLEEK GSSEEPVYLE KLLYDRALEI 800
    SKMAAHMELK GENLYNCELA YATSLWMLET SLDDDDFTNA YGDYPFKTNI 850
    HLKSNDVEDK EKYHSVLDEN DRIIIRKYID SIANRLKILR QKMNHQN 897
    Length:897
    Mass (Da):101,717
    Last modified:October 1, 1996 - v1
    Checksum:i7F4C785AA3A7CC46
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X91489 Genomic DNA. Translation: CAA62794.1.
    D29991 Genomic DNA. Translation: BAA21481.1.
    Z72702 Genomic DNA. Translation: CAA96892.1.
    BK006941 Genomic DNA. Translation: DAA07934.1.
    PIRiS61137.
    RefSeqiNP_011335.1. NM_001181045.1.

    Genome annotation databases

    EnsemblFungiiYGL180W; YGL180W; YGL180W.
    GeneIDi852695.
    KEGGisce:YGL180W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X91489 Genomic DNA. Translation: CAA62794.1 .
    D29991 Genomic DNA. Translation: BAA21481.1 .
    Z72702 Genomic DNA. Translation: CAA96892.1 .
    BK006941 Genomic DNA. Translation: DAA07934.1 .
    PIRi S61137.
    RefSeqi NP_011335.1. NM_001181045.1.

    3D structure databases

    ProteinModelPortali P53104.
    SMRi P53104. Positions 17-358.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33074. 374 interactions.
    DIPi DIP-1192N.
    IntActi P53104. 21 interactions.
    MINTi MINT-405956.
    STRINGi 4932.YGL180W.

    Protein family/group databases

    TCDBi 9.A.15.1.1. the autophagy-related phagophore-formation transporter (apt) family.

    Proteomic databases

    MaxQBi P53104.
    PaxDbi P53104.
    PRIDEi P53104.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL180W ; YGL180W ; YGL180W .
    GeneIDi 852695.
    KEGGi sce:YGL180W.

    Organism-specific databases

    CYGDi YGL180w.
    SGDi S000003148. ATG1.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00750000117559.
    HOGENOMi HOG000246715.
    KOi K08269.
    OMAi EKLMYDR.
    OrthoDBi EOG7CCC0Z.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30667-MONOMER.
    BRENDAi 2.7.11.1. 984.

    Miscellaneous databases

    NextBioi 972032.
    PROi P53104.

    Gene expression databases

    Genevestigatori P53104.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    IPR022708. Ser/Thr_kinase_C.
    [Graphical view ]
    Pfami PF12063. DUF3543. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequencing of a 40.5 kb fragment located on the left arm of chromosome VII from Saccharomyces cerevisiae."
      Coglievina M., Klima R., Bertani I., Delneri D., Zaccaria P., Bruschi C.V.
      Yeast 13:55-64(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    2. "Apg1p, a novel protein kinase required for the autophagic process in Saccharomyces cerevisiae."
      Matsuura A., Tsukada M., Wada Y., Ohsumi Y.
      Gene 192:245-250(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, PHOSPHORYLATION, MUTAGENESIS OF ASP-211 AND GLU-237.
      Strain: ATCC 26109 / X2180.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae."
      Tsukada M., Ohsumi Y.
      FEBS Lett. 333:169-174(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Genetic and phenotypic overlap between autophagy and the cytoplasm to vacuole protein targeting pathway."
      Harding T.M., Hefner-Gravink A., Thumm M., Klionsky D.J.
      J. Biol. Chem. 271:17621-17624(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "AUT3, a serine/threonine kinase gene, is essential for autophagocytosis in Saccharomyces cerevisiae."
      Straub M., Bredschneider M., Thumm M.
      J. Bacteriol. 179:3875-3883(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    8. "Apg13p and Vac8p are part of a complex of phosphoproteins that are required for cytoplasm to vacuole targeting."
      Scott S.V., Nice D.C. III, Nau J.J., Weisman L.S., Kamada Y., Keizer-Gunnink I., Funakoshi T., Veenhuis M., Ohsumi Y., Klionsky D.J.
      J. Biol. Chem. 275:25840-25849(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    9. "Tor-mediated induction of autophagy via an Apg1 protein kinase complex."
      Kamada Y., Funakoshi T., Shintani T., Nagano K., Ohsumi M., Ohsumi Y.
      J. Cell Biol. 150:1507-1513(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ENZYME REGULATION, MUTAGENESIS OF LYS-54, INTERACTION WITH ATG11; ATG13 AND ATG17.
    10. "The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation."
      Suzuki K., Kirisako T., Kamada Y., Mizushima N., Noda T., Ohsumi Y.
      EMBO J. 20:5971-5981(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    11. "Cvt9/Gsa9 functions in sequestering selective cytosolic cargo destined for the vacuole."
      Kim J., Kamada Y., Stromhaug P.E., Guan J., Hefner-Gravink A., Baba M., Scott S.V., Ohsumi Y., Dunn W.A. Jr., Klionsky D.J.
      J. Cell Biol. 153:381-396(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ATG11.
    12. "Antagonistic controls of autophagy and glycogen accumulation by Snf1p, the yeast homolog of AMP-activated protein kinase, and the cyclin-dependent kinase Pho85p."
      Wang Z., Wilson W.A., Fujino M.A., Roach P.J.
      Mol. Cell. Biol. 21:5742-5752(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    13. "Starvation-induced degradation of yeast hexose transporter Hxt7p is dependent on endocytosis, autophagy and the terminal sequences of the permease."
      Krampe S., Boles E.
      FEBS Lett. 513:193-196(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    14. Cited for: NOMENCLATURE.
    15. "Chemical genetic analysis of Apg1 reveals a non-kinase role in the induction of autophagy."
      Abeliovich H., Zhang C., Dunn W.A. Jr., Shokat K.M., Klionsky D.J.
      Mol. Biol. Cell 14:477-490(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF LYS-54; MET-102; ASN-884 AND LEU-886.
    16. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    17. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    18. "The Atg1-Atg13 complex regulates Atg9 and Atg23 retrieval transport from the pre-autophagosomal structure."
      Reggiori F., Tucker K.A., Stromhaug P.E., Klionsky D.J.
      Dev. Cell 6:79-90(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    19. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-677, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    20. "Overexpression of autophagy-related genes inhibits yeast filamentous growth."
      Ma J., Jin R., Dobry C.J., Lawson S.K., Kumar A.
      Autophagy 3:604-609(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    21. "Hierarchy of Atg proteins in pre-autophagosomal structure organization."
      Suzuki K., Kubota Y., Sekito T., Ohsumi Y.
      Genes Cells 12:209-218(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    22. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    23. "Protein kinase A and Sch9 cooperatively regulate induction of autophagy in Saccharomyces cerevisiae."
      Yorimitsu T., Zaman S., Broach J.R., Klionsky D.J.
      Mol. Biol. Cell 18:4180-4189(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT SER-508 AND SER-515 BY PKA, MUTAGENESIS OF SER-508 AND SER-515, FUNCTION.
    24. "Localization of autophagy-related proteins in yeast using a versatile plasmid-based resource of fluorescent protein fusions."
      Ma J., Bharucha N., Dobry C.J., Frisch R.L., Lawson S., Kumar A.
      Autophagy 4:792-800(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    25. "Mitophagy in yeast occurs through a selective mechanism."
      Kanki T., Klionsky D.J.
      J. Biol. Chem. 283:32386-32393(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    26. "In vivo reconstitution of autophagy in Saccharomyces cerevisiae."
      Cao Y., Cheong H., Song H., Klionsky D.J.
      J. Cell Biol. 182:703-713(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    27. "The Atg1 kinase complex is involved in the regulation of protein recruitment to initiate sequestering vesicle formation for nonspecific autophagy in Saccharomyces cerevisiae."
      Cheong H., Nair U., Geng J., Klionsky D.J.
      Mol. Biol. Cell 19:668-681(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH ATG13 AND ATG17.
    28. "Piecemeal microautophagy of the nucleus requires the core macroautophagy genes."
      Krick R., Muehe Y., Prick T., Bremer S., Schlotterhose P., Eskelinen E.L., Millen J., Goldfarb D.S., Thumm M.
      Mol. Biol. Cell 19:4492-4505(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    29. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-390, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    30. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-635; SER-638 AND SER-647, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    31. "Autophosphorylation within the Atg1 activation loop is required for both kinase activity and the induction of autophagy in Saccharomyces cerevisiae."
      Yeh Y.Y., Wrasman K., Herman P.K.
      Genetics 185:871-882(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT THR-226 BY AUTOCATALYSIS, MUTAGENESIS OF THR-226, ENZYME REGULATION, FUNCTION.
    32. "An Atg9-containing compartment that functions in the early steps of autophagosome biogenesis."
      Mari M., Griffith J., Rieter E., Krishnappa L., Klionsky D.J., Reggiori F.
      J. Cell Biol. 190:1005-1022(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    33. "The identification and analysis of phosphorylation sites on the Atg1 protein kinase."
      Yeh Y.Y., Shah K.H., Chou C.C., Hsiao H.H., Wrasman K.M., Stephan J.S., Stamatakos D., Khoo K.H., Herman P.K.
      Autophagy 7:716-726(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT SER-34; THR-129; SER-304; SER-365; SER-390; SER-515; SER-533; SER-551; SER-552; THR-590; SER-621; SER-677; SER-680; SER-683; SER-769 AND SER-783, MUTAGENESIS OF SER-34; SER-551; SER-552; SER-621; SER-677; SER-680; SER-683; SER-769 AND SER-783, FUNCTION.
    34. "Bidirectional regulation between TORC1 and autophagy in Saccharomyces cerevisiae."
      Shin C.S., Huh W.K.
      Autophagy 7:854-862(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    35. "A new autophagy-related checkpoint in the degradation of an ERAD-M target."
      Kario E., Amar N., Elazar Z., Navon A.
      J. Biol. Chem. 286:11479-11491(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    36. "An Atg13 protein-mediated self-association of the Atg1 protein kinase is important for the induction of autophagy."
      Yeh Y.Y., Shah K.H., Herman P.K.
      J. Biol. Chem. 286:28931-28939(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, INTERACTION WITH ATG13, FUNCTION.
    37. "Two MAPK-signaling pathways are required for mitophagy in Saccharomyces cerevisiae."
      Mao K., Wang K., Zhao M., Xu T., Klionsky D.J.
      J. Cell Biol. 193:755-767(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    38. Cited for: FUNCTION.
    39. Cited for: INTERACTION WITH ATG13 AND ATG8, DOMAIN, FUNCTION, SUBCELLULAR LOCATION.
    40. "Aggregate clearance of alpha-synuclein in Saccharomyces cerevisiae depends more on autophagosome and vacuole function than on the proteasome."
      Petroi D., Popova B., Taheri-Talesh N., Irniger S., Shahpasandzadeh H., Zweckstetter M., Outeiro T.F., Braus G.H.
      J. Biol. Chem. 287:27567-27579(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    41. "The autophagy-related protein kinase Atg1 interacts with the ubiquitin-like protein Atg8 via the Atg8 family interacting motif to facilitate autophagosome formation."
      Nakatogawa H., Ohbayashi S., Sakoh-Nakatogawa M., Kakuta S., Suzuki S.W., Kirisako H., Kondo-Kakuta C., Noda N.N., Yamamoto H., Ohsumi Y.
      J. Biol. Chem. 287:28503-28507(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ATG8, SUBCELLULAR LOCATION, DOMAIN, MUTAGENESIS OF TYR-429 AND VAL-432.
    42. "Phosphatidylinositol 4-kinases are required for autophagic membrane trafficking."
      Wang K., Yang Z., Liu X., Mao K., Nair U., Klionsky D.J.
      J. Biol. Chem. 287:37964-37972(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    43. "A late form of nucleophagy in Saccharomyces cerevisiae."
      Mijaljica D., Prescott M., Devenish R.J.
      PLoS ONE 7:E40013-E40013(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    44. "Increased levels of reduced cytochrome b and mitophagy components are required to trigger nonspecific autophagy following induced mitochondrial dysfunction."
      Deffieu M., Bhatia-Kissova I., Salin B., Klionsky D.J., Pinson B., Manon S., Camougrand N.
      J. Cell Sci. 126:415-426(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    45. "Fine mapping of autophagy-related proteins during autophagosome formation in Saccharomyces cerevisiae."
      Suzuki K., Akioka M., Kondo-Kakuta C., Yamamoto H., Ohsumi Y.
      J. Cell Sci. 126:2534-2544(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, FUNCTION, MUTAGENESIS OF LYS-54 AND ASP-211.
    46. "The role of autophagy in genome stability through suppression of abnormal mitosis under starvation."
      Matsui A., Kamada Y., Matsuura A.
      PLoS Genet. 9:E1003245-E1003245(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiATG1_YEAST
    AccessioniPrimary (citable) accession number: P53104
    Secondary accession number(s): D6VTX3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 141 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1070 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3