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P53104 (ATG1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 138. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase ATG1

EC=2.7.11.1
Alternative name(s):
Autophagy protein 3
Autophagy-related protein 1
Cytoplasm to vacuole targeting protein 10
Gene names
Name:ATG1
Synonyms:APG1, AUT3, CVT10
Ordered Locus Names:YGL180W
ORF Names:G1615
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length897 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Serine/threonine protein kinase involved in the cytoplasm to vacuole transport (Cvt) and found to be essential in autophagy, where it is required for the formation of autophagosomes. Involved in the clearance of protein aggregates which cannot be efficiently cleared by the proteasome. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Also involved in endoplasmic reticulum-specific autophagic process, in selective removal of ER-associated degradation (ERAD) substrates. Plays a key role in ATG9 and ATG23 cycling through the pre-autophagosomal structure and is necessary to promote ATG18 binding to ATG9. Finally, ATG1 is also required for the maintenance of cell viability under starvation and for glycogen storage during stationary phase. Plays a role in genome stability through suppression of abnormal mitosis under starvation, and in regulation of filamentous growth. Ref.2 Ref.5 Ref.6 Ref.7 Ref.9 Ref.10 Ref.12 Ref.13 Ref.15 Ref.18 Ref.20 Ref.21 Ref.23 Ref.25 Ref.26 Ref.27 Ref.28 Ref.31 Ref.32 Ref.33 Ref.34 Ref.35 Ref.36 Ref.37 Ref.38 Ref.39 Ref.40 Ref.42 Ref.43 Ref.44 Ref.45 Ref.46

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Activated by hypophosphorylated form of ATG13 (present in nitrogen starvation conditions). Also activated by autophopsphorylation of Thr-226 and inhibited by phosphorylation of Ser-34. Ref.9 Ref.31

Subunit structure

Homodimer. Dimerization requires the presence of ATG13. Forms a ternary complex with ATG13 and ATG17. Interacts also with ATG11. Ref.9 Ref.11 Ref.27 Ref.36 Ref.39 Ref.41

Subcellular location

Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein. Note: Formes punctate structures in starvation conditions only when ATG13 and ATG17 were both present. Localizes to both the isolation membrane (IM) and the vacuole-isolation membrane contact site (VICS) during IM expansion. The IM is a membrane sac generated from the pre-autophagosomal structure that ultimately expands to become a mature autophagosome. Ref.7 Ref.8 Ref.10 Ref.16 Ref.21 Ref.24 Ref.26 Ref.39 Ref.41 Ref.45

Domain

The LIR motif is required for the interaction with ATG8 and for the association of ATG1 with autophagosomes. Ref.39 Ref.41

Post-translational modification

Autophosphorylated at Thr-226 and Ser-390. The phosphorylation state may play a role in the induction of protein degradation upon starvation. Phosphorylation at Thr-226 within the activation loop is required for protein kinase activity whereas phosphorylation at Ser-34 leads to inhibition of kinase activity. Phosphorylation of Ser-508 and Ser-515 by PKA is required to induce autophagy but not for kinase activity. Ref.2 Ref.23 Ref.31 Ref.33

Miscellaneous

Present with 1070 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.

Contains 1 protein kinase domain.

Ontologies

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 897897Serine/threonine-protein kinase ATG1
PRO_0000085655

Regions

Domain24 – 325302Protein kinase
Nucleotide binding30 – 389ATP By similarity
Region880 – 8867Required for Cvt trafficking
Motif429 – 4324LIR
Compositional bias542 – 5487Poly-Gln

Sites

Active site1721Proton acceptor By similarity
Binding site541ATP By similarity

Amino acid modifications

Modified residue341Phosphoserine Ref.33
Modified residue1291Phosphothreonine Ref.33
Modified residue2261Phosphothreonine; by autocatalysis Ref.31
Modified residue3041Phosphoserine Ref.33
Modified residue3651Phosphoserine Ref.33
Modified residue3901Phosphoserine Ref.29 Ref.33
Modified residue5081Phosphoserine; by PKA Ref.23
Modified residue5151Phosphoserine; by PKA Ref.23 Ref.33
Modified residue5331Phosphoserine Ref.33
Modified residue5511Phosphoserine Ref.33
Modified residue5521Phosphoserine Ref.33
Modified residue5901Phosphothreonine Ref.33
Modified residue6211Phosphoserine Ref.33
Modified residue6351Phosphoserine Ref.30
Modified residue6381Phosphoserine Ref.30
Modified residue6471Phosphoserine Ref.30
Modified residue6771Phosphoserine Ref.19 Ref.33
Modified residue6801Phosphoserine Ref.33
Modified residue6831Phosphoserine Ref.33
Modified residue7691Phosphoserine Ref.33
Modified residue7831Phosphoserine Ref.33

Experimental info

Mutagenesis341S → D or E: Impairs kinase activity. Ref.33
Mutagenesis541K → A: Defect in the Cvt pathway. Ref.9 Ref.15 Ref.45
Mutagenesis1021M → A: Enables the binding of the inhibitor of the kinase activity 1-NA-PP1. Ref.15
Mutagenesis2111D → A: Loss of cell viability under starvation. Ref.2 Ref.45
Mutagenesis2261T → E: Leads to constitutive autophosphorylation activity. Ref.31
Mutagenesis2371E → R: Loss of cell viability under starvation. Ref.2
Mutagenesis4291Y → A: Impairs interaction with ATG8 and decreases autophagy efficiency; when associated with A-432. Ref.41
Mutagenesis4321V → A: Impairs interaction with ATG8 and decreases autophagy efficiency; when associated with A-429. Ref.41
Mutagenesis5081S → A: Impairs autophagic activity; when associated with A-515. Ref.23
Mutagenesis5151S → A: Impairs autophagic activity; when associated with A-508. Ref.23
Mutagenesis5511S → A: Decreases autophagic activity. Ref.33
Mutagenesis5521S → A: Decreases autophagic activity. Ref.33
Mutagenesis6211S → A: Decreases autophagic activity. Ref.33
Mutagenesis6771S → A: Decreases autophagic activity. Ref.33
Mutagenesis6801S → A: Decreases autophagic activity. Ref.33
Mutagenesis6831S → A: Decreases autophagic activity. Ref.33
Mutagenesis7691S → A: Decreases autophagic activity. Ref.33
Mutagenesis7831S → A: Decreases autophagic activity. Ref.33
Mutagenesis8841N → A: No effect. Ref.15
Mutagenesis8861L → G: Cvt pathway specific block. Ref.15

Sequences

Sequence LengthMass (Da)Tools
P53104 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 7F4C785AA3A7CC46

FASTA897101,717
        10         20         30         40         50         60 
MGDIKNKDHT TSVNHNLMAS AGNYTAEKEI GKGSFATVYR GHLTSDKSQH VAIKEVSRAK 

        70         80         90        100        110        120 
LKNKKLLENL EIEIAILKKI KHPHIVGLID CERTSTDFYL IMEYCALGDL TFLLKRRKEL 

       130        140        150        160        170        180 
MENHPLLRTV FEKYPPPSEN HNGLHRAFVL SYLQQLASAL KFLRSKNLVH RDIKPQNLLL 

       190        200        210        220        230        240 
STPLIGYHDS KSFHELGFVG IYNLPILKIA DFGFARFLPN TSLAETLCGS PLYMAPEILN 

       250        260        270        280        290        300 
YQKYNAKADL WSVGTVVFEM CCGTPPFRAS NHLELFKKIK RANDVITFPS YCNIEPELKE 

       310        320        330        340        350        360 
LICSLLTFDP AQRIGFEEFF ANKVVNEDLS SYELEDDLPE LESKSKGIVE SNMFVSEYLS 

       370        380        390        400        410        420 
KQPKSPNSNL AGHQSMADNP AELSDALKNS NILTAPAVKT DHTQAVDKKA SNNKYHNSLV 

       430        440        450        460        470        480 
SDRSFEREYV VVEKKSVEVN SLADEVAQAG FNPNPIKHPT STQNQNVLLN EQFSPNNQQY 

       490        500        510        520        530        540 
FQNQGENPRL LRATSSSSGG SDGSRRPSLV DRRLSISSLN PSNALSRALG IASTRLFGGA 

       550        560        570        580        590        600 
NQQQQQQQIT SSPPYSQTLL NSQLFHELTE NIILRIDHLQ HPETLKLDNT NIVSILESLA 

       610        620        630        640        650        660 
AKAFVVYSYA EVKFSQIVPL STTLKGMANF ENRRSMDSNA IAEEQDSDDA EEEDETLKKY 

       670        680        690        700        710        720 
KEDCLSTKTF GKGRTLSATS QLSATFNKLP RSEMILLCNE AIVLYMKALS ILSKSMQVTS 

       730        740        750        760        770        780 
NWWYESQEKS CSLRVNVLVQ WLREKFNECL EKADFLRLKI NDLRFKHASE VAENQTLEEK 

       790        800        810        820        830        840 
GSSEEPVYLE KLLYDRALEI SKMAAHMELK GENLYNCELA YATSLWMLET SLDDDDFTNA 

       850        860        870        880        890 
YGDYPFKTNI HLKSNDVEDK EKYHSVLDEN DRIIIRKYID SIANRLKILR QKMNHQN 

« Hide

References

« Hide 'large scale' references
[1]"Sequencing of a 40.5 kb fragment located on the left arm of chromosome VII from Saccharomyces cerevisiae."
Coglievina M., Klima R., Bertani I., Delneri D., Zaccaria P., Bruschi C.V.
Yeast 13:55-64(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]"Apg1p, a novel protein kinase required for the autophagic process in Saccharomyces cerevisiae."
Matsuura A., Tsukada M., Wada Y., Ohsumi Y.
Gene 192:245-250(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, PHOSPHORYLATION, MUTAGENESIS OF ASP-211 AND GLU-237.
Strain: ATCC 26109 / X2180.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae."
Tsukada M., Ohsumi Y.
FEBS Lett. 333:169-174(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Genetic and phenotypic overlap between autophagy and the cytoplasm to vacuole protein targeting pathway."
Harding T.M., Hefner-Gravink A., Thumm M., Klionsky D.J.
J. Biol. Chem. 271:17621-17624(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"AUT3, a serine/threonine kinase gene, is essential for autophagocytosis in Saccharomyces cerevisiae."
Straub M., Bredschneider M., Thumm M.
J. Bacteriol. 179:3875-3883(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[8]"Apg13p and Vac8p are part of a complex of phosphoproteins that are required for cytoplasm to vacuole targeting."
Scott S.V., Nice D.C. III, Nau J.J., Weisman L.S., Kamada Y., Keizer-Gunnink I., Funakoshi T., Veenhuis M., Ohsumi Y., Klionsky D.J.
J. Biol. Chem. 275:25840-25849(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[9]"Tor-mediated induction of autophagy via an Apg1 protein kinase complex."
Kamada Y., Funakoshi T., Shintani T., Nagano K., Ohsumi M., Ohsumi Y.
J. Cell Biol. 150:1507-1513(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ENZYME REGULATION, MUTAGENESIS OF LYS-54, INTERACTION WITH ATG11; ATG13 AND ATG17.
[10]"The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation."
Suzuki K., Kirisako T., Kamada Y., Mizushima N., Noda T., Ohsumi Y.
EMBO J. 20:5971-5981(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[11]"Cvt9/Gsa9 functions in sequestering selective cytosolic cargo destined for the vacuole."
Kim J., Kamada Y., Stromhaug P.E., Guan J., Hefner-Gravink A., Baba M., Scott S.V., Ohsumi Y., Dunn W.A. Jr., Klionsky D.J.
J. Cell Biol. 153:381-396(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ATG11.
[12]"Antagonistic controls of autophagy and glycogen accumulation by Snf1p, the yeast homolog of AMP-activated protein kinase, and the cyclin-dependent kinase Pho85p."
Wang Z., Wilson W.A., Fujino M.A., Roach P.J.
Mol. Cell. Biol. 21:5742-5752(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[13]"Starvation-induced degradation of yeast hexose transporter Hxt7p is dependent on endocytosis, autophagy and the terminal sequences of the permease."
Krampe S., Boles E.
FEBS Lett. 513:193-196(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[14]"A unified nomenclature for yeast autophagy-related genes."
Klionsky D.J., Cregg J.M., Dunn W.A. Jr., Emr S.D., Sakai Y., Sandoval I.V., Sibirny A., Subramani S., Thumm M., Veenhuis M., Ohsumi Y.
Dev. Cell 5:539-545(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE.
[15]"Chemical genetic analysis of Apg1 reveals a non-kinase role in the induction of autophagy."
Abeliovich H., Zhang C., Dunn W.A. Jr., Shokat K.M., Klionsky D.J.
Mol. Biol. Cell 14:477-490(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF LYS-54; MET-102; ASN-884 AND LEU-886.
[16]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[17]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[18]"The Atg1-Atg13 complex regulates Atg9 and Atg23 retrieval transport from the pre-autophagosomal structure."
Reggiori F., Tucker K.A., Stromhaug P.E., Klionsky D.J.
Dev. Cell 6:79-90(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[19]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-677, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: YAL6B.
[20]"Overexpression of autophagy-related genes inhibits yeast filamentous growth."
Ma J., Jin R., Dobry C.J., Lawson S.K., Kumar A.
Autophagy 3:604-609(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[21]"Hierarchy of Atg proteins in pre-autophagosomal structure organization."
Suzuki K., Kubota Y., Sekito T., Ohsumi Y.
Genes Cells 12:209-218(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[22]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[23]"Protein kinase A and Sch9 cooperatively regulate induction of autophagy in Saccharomyces cerevisiae."
Yorimitsu T., Zaman S., Broach J.R., Klionsky D.J.
Mol. Biol. Cell 18:4180-4189(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-508 AND SER-515 BY PKA, MUTAGENESIS OF SER-508 AND SER-515, FUNCTION.
[24]"Localization of autophagy-related proteins in yeast using a versatile plasmid-based resource of fluorescent protein fusions."
Ma J., Bharucha N., Dobry C.J., Frisch R.L., Lawson S., Kumar A.
Autophagy 4:792-800(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[25]"Mitophagy in yeast occurs through a selective mechanism."
Kanki T., Klionsky D.J.
J. Biol. Chem. 283:32386-32393(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[26]"In vivo reconstitution of autophagy in Saccharomyces cerevisiae."
Cao Y., Cheong H., Song H., Klionsky D.J.
J. Cell Biol. 182:703-713(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[27]"The Atg1 kinase complex is involved in the regulation of protein recruitment to initiate sequestering vesicle formation for nonspecific autophagy in Saccharomyces cerevisiae."
Cheong H., Nair U., Geng J., Klionsky D.J.
Mol. Biol. Cell 19:668-681(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH ATG13 AND ATG17.
[28]"Piecemeal microautophagy of the nucleus requires the core macroautophagy genes."
Krick R., Muehe Y., Prick T., Bremer S., Schlotterhose P., Eskelinen E.L., Millen J., Goldfarb D.S., Thumm M.
Mol. Biol. Cell 19:4492-4505(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[29]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-390, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[30]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-635; SER-638 AND SER-647, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[31]"Autophosphorylation within the Atg1 activation loop is required for both kinase activity and the induction of autophagy in Saccharomyces cerevisiae."
Yeh Y.Y., Wrasman K., Herman P.K.
Genetics 185:871-882(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT THR-226 BY AUTOCATALYSIS, MUTAGENESIS OF THR-226, ENZYME REGULATION, FUNCTION.
[32]"An Atg9-containing compartment that functions in the early steps of autophagosome biogenesis."
Mari M., Griffith J., Rieter E., Krishnappa L., Klionsky D.J., Reggiori F.
J. Cell Biol. 190:1005-1022(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[33]"The identification and analysis of phosphorylation sites on the Atg1 protein kinase."
Yeh Y.Y., Shah K.H., Chou C.C., Hsiao H.H., Wrasman K.M., Stephan J.S., Stamatakos D., Khoo K.H., Herman P.K.
Autophagy 7:716-726(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-34; THR-129; SER-304; SER-365; SER-390; SER-515; SER-533; SER-551; SER-552; THR-590; SER-621; SER-677; SER-680; SER-683; SER-769 AND SER-783, MUTAGENESIS OF SER-34; SER-551; SER-552; SER-621; SER-677; SER-680; SER-683; SER-769 AND SER-783, FUNCTION.
[34]"Bidirectional regulation between TORC1 and autophagy in Saccharomyces cerevisiae."
Shin C.S., Huh W.K.
Autophagy 7:854-862(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[35]"A new autophagy-related checkpoint in the degradation of an ERAD-M target."
Kario E., Amar N., Elazar Z., Navon A.
J. Biol. Chem. 286:11479-11491(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[36]"An Atg13 protein-mediated self-association of the Atg1 protein kinase is important for the induction of autophagy."
Yeh Y.Y., Shah K.H., Herman P.K.
J. Biol. Chem. 286:28931-28939(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT, INTERACTION WITH ATG13, FUNCTION.
[37]"Two MAPK-signaling pathways are required for mitophagy in Saccharomyces cerevisiae."
Mao K., Wang K., Zhao M., Xu T., Klionsky D.J.
J. Cell Biol. 193:755-767(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[38]"14-3-3 protects against stress-induced apoptosis."
Clapp C., Portt L., Khoury C., Sheibani S., Norman G., Ebner P., Eid R., Vali H., Mandato C.A., Madeo F., Greenwood M.T.
Cell Death Dis. 3:E348-E348(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[39]"Binding of the Atg1/ULK1 kinase to the ubiquitin-like protein Atg8 regulates autophagy."
Kraft C., Kijanska M., Kalie E., Siergiejuk E., Lee S.S., Semplicio G., Stoffel I., Brezovich A., Verma M., Hansmann I., Ammerer G., Hofmann K., Tooze S., Peter M.
EMBO J. 31:3691-3703(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ATG13 AND ATG8, DOMAIN, FUNCTION, SUBCELLULAR LOCATION.
[40]"Aggregate clearance of alpha-synuclein in Saccharomyces cerevisiae depends more on autophagosome and vacuole function than on the proteasome."
Petroi D., Popova B., Taheri-Talesh N., Irniger S., Shahpasandzadeh H., Zweckstetter M., Outeiro T.F., Braus G.H.
J. Biol. Chem. 287:27567-27579(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[41]"The autophagy-related protein kinase Atg1 interacts with the ubiquitin-like protein Atg8 via the Atg8 family interacting motif to facilitate autophagosome formation."
Nakatogawa H., Ohbayashi S., Sakoh-Nakatogawa M., Kakuta S., Suzuki S.W., Kirisako H., Kondo-Kakuta C., Noda N.N., Yamamoto H., Ohsumi Y.
J. Biol. Chem. 287:28503-28507(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ATG8, SUBCELLULAR LOCATION, DOMAIN, MUTAGENESIS OF TYR-429 AND VAL-432.
[42]"Phosphatidylinositol 4-kinases are required for autophagic membrane trafficking."
Wang K., Yang Z., Liu X., Mao K., Nair U., Klionsky D.J.
J. Biol. Chem. 287:37964-37972(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[43]"A late form of nucleophagy in Saccharomyces cerevisiae."
Mijaljica D., Prescott M., Devenish R.J.
PLoS ONE 7:E40013-E40013(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[44]"Increased levels of reduced cytochrome b and mitophagy components are required to trigger nonspecific autophagy following induced mitochondrial dysfunction."
Deffieu M., Bhatia-Kissova I., Salin B., Klionsky D.J., Pinson B., Manon S., Camougrand N.
J. Cell Sci. 126:415-426(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[45]"Fine mapping of autophagy-related proteins during autophagosome formation in Saccharomyces cerevisiae."
Suzuki K., Akioka M., Kondo-Kakuta C., Yamamoto H., Ohsumi Y.
J. Cell Sci. 126:2534-2544(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, FUNCTION, MUTAGENESIS OF LYS-54 AND ASP-211.
[46]"The role of autophagy in genome stability through suppression of abnormal mitosis under starvation."
Matsui A., Kamada Y., Matsuura A.
PLoS Genet. 9:E1003245-E1003245(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X91489 Genomic DNA. Translation: CAA62794.1.
D29991 Genomic DNA. Translation: BAA21481.1.
Z72702 Genomic DNA. Translation: CAA96892.1.
BK006941 Genomic DNA. Translation: DAA07934.1.
PIRS61137.
RefSeqNP_011335.1. NM_001181045.1.

3D structure databases

ProteinModelPortalP53104.
SMRP53104. Positions 23-379.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33074. 365 interactions.
DIPDIP-1192N.
IntActP53104. 21 interactions.
MINTMINT-405956.
STRING4932.YGL180W.

Protein family/group databases

TCDB9.A.15.1.1. the autophagy-related phagophore-formation transporter (apt) family.

Proteomic databases

PaxDbP53104.
PRIDEP53104.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGL180W; YGL180W; YGL180W.
GeneID852695.
KEGGsce:YGL180W.

Organism-specific databases

CYGDYGL180w.
SGDS000003148. ATG1.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00750000117559.
HOGENOMHOG000246715.
KOK08269.
OMAEKLMYDR.
OrthoDBEOG7CCC0Z.

Enzyme and pathway databases

BioCycYEAST:G3O-30667-MONOMER.
BRENDA2.7.11.1. 984.

Gene expression databases

GenevestigatorP53104.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR022708. Ser/Thr_kinase_C.
[Graphical view]
PfamPF12063. DUF3543. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio972032.
PROP53104.

Entry information

Entry nameATG1_YEAST
AccessionPrimary (citable) accession number: P53104
Secondary accession number(s): D6VTX3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families