Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P53104

- ATG1_YEAST

UniProt

P53104 - ATG1_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Serine/threonine-protein kinase ATG1

Gene

ATG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Serine/threonine protein kinase involved in the cytoplasm to vacuole transport (Cvt) and found to be essential in autophagy, where it is required for the formation of autophagosomes. Involved in the clearance of protein aggregates which cannot be efficiently cleared by the proteasome. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Also involved in endoplasmic reticulum-specific autophagic process, in selective removal of ER-associated degradation (ERAD) substrates. Plays a key role in ATG9 and ATG23 cycling through the pre-autophagosomal structure and is necessary to promote ATG18 binding to ATG9 through phosphorylation of ATG9. Finally, ATG1 is also required for the maintenance of cell viability under starvation and for glycogen storage during stationary phase. Plays a role in genome stability through suppression of abnormal mitosis under starvation, and in regulation of filamentous growth.33 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Enzyme regulationi

Activated by hypophosphorylated form of ATG13 (present in nitrogen starvation conditions). Also activated by autophopsphorylation of Thr-226 and inhibited by phosphorylation of Ser-34.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei54 – 541ATPPROSITE-ProRule annotation
Active sitei172 – 1721Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi30 – 389ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein serine/threonine kinase activity Source: SGD

GO - Biological processi

  1. autophagic vacuole assembly Source: SGD
  2. autophagy Source: SGD
  3. CVT pathway Source: SGD
  4. late nucleophagy Source: SGD
  5. mitochondrion degradation Source: SGD
  6. piecemeal microautophagy of nucleus Source: SGD
  7. protein autophosphorylation Source: SGD
  8. protein phosphorylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Autophagy, Protein transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30667-MONOMER.
BRENDAi2.7.11.1. 984.

Protein family/group databases

TCDBi9.A.15.1.1. the autophagy-related phagophore-formation transporter (apt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase ATG1Curated (EC:2.7.11.11 Publication)
Alternative name(s):
Autophagy protein 31 Publication
Autophagy-related protein 11 Publication
Cytoplasm to vacuole targeting protein 101 Publication
Gene namesi
Name:ATG11 Publication
Synonyms:APG11 Publication, AUT31 Publication, CVT101 Publication
Ordered Locus Names:YGL180WImported
ORF Names:G1615
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGL180w.
SGDiS000003148. ATG1.

Subcellular locationi

Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein
Note: Formes punctate structures in starvation conditions only when ATG13 and ATG17 were both present. Localizes to both the isolation membrane (IM) and the vacuole-isolation membrane contact site (VICS) during IM expansion. The IM is a membrane sac generated from the pre-autophagosomal structure that ultimately expands to become a mature autophagosome.

GO - Cellular componenti

  1. ATG1/UKL1 signaling complex Source: SGD
  2. autophagic vacuole membrane Source: SGD
  3. cytosol Source: SGD
  4. extrinsic component of autophagic vacuole membrane Source: UniProtKB
  5. pre-autophagosomal structure Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi34 – 341S → D or E: Impairs kinase activity. 1 Publication
Mutagenesisi54 – 541K → A: Defect in the Cvt pathway. 3 Publications
Mutagenesisi102 – 1021M → A: Enables the binding of the inhibitor of the kinase activity 1-NA-PP1. 1 Publication
Mutagenesisi211 – 2111D → A: Loss of cell viability under starvation. 2 Publications
Mutagenesisi226 – 2261T → E: Leads to constitutive autophosphorylation activity. 1 Publication
Mutagenesisi237 – 2371E → R: Loss of cell viability under starvation. 1 Publication
Mutagenesisi429 – 4291Y → A: Impairs interaction with ATG8 and decreases autophagy efficiency; when associated with A-432. 1 Publication
Mutagenesisi432 – 4321V → A: Impairs interaction with ATG8 and decreases autophagy efficiency; when associated with A-429. 1 Publication
Mutagenesisi508 – 5081S → A: Impairs autophagic activity; when associated with A-515. 1 Publication
Mutagenesisi515 – 5151S → A: Impairs autophagic activity; when associated with A-508. 1 Publication
Mutagenesisi551 – 5511S → A: Decreases autophagic activity. 1 Publication
Mutagenesisi552 – 5521S → A: Decreases autophagic activity. 1 Publication
Mutagenesisi621 – 6211S → A: Decreases autophagic activity. 1 Publication
Mutagenesisi677 – 6771S → A: Decreases autophagic activity. 1 Publication
Mutagenesisi680 – 6801S → A: Decreases autophagic activity. 1 Publication
Mutagenesisi683 – 6831S → A: Decreases autophagic activity. 1 Publication
Mutagenesisi769 – 7691S → A: Decreases autophagic activity. 1 Publication
Mutagenesisi783 – 7831S → A: Decreases autophagic activity. 1 Publication
Mutagenesisi884 – 8841N → A: No effect. 1 Publication
Mutagenesisi886 – 8861L → G: Cvt pathway specific block. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 897897Serine/threonine-protein kinase ATG1PRO_0000085655Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei34 – 341Phosphoserine1 Publication
Modified residuei129 – 1291Phosphothreonine1 Publication
Modified residuei226 – 2261Phosphothreonine; by autocatalysis1 Publication
Modified residuei304 – 3041Phosphoserine1 Publication
Modified residuei365 – 3651Phosphoserine1 Publication
Modified residuei390 – 3901Phosphoserine2 Publications
Modified residuei508 – 5081Phosphoserine; by PKA1 Publication
Modified residuei515 – 5151Phosphoserine; by PKA2 Publications
Modified residuei533 – 5331Phosphoserine1 Publication
Modified residuei551 – 5511Phosphoserine1 Publication
Modified residuei552 – 5521Phosphoserine1 Publication
Modified residuei590 – 5901Phosphothreonine1 Publication
Modified residuei621 – 6211Phosphoserine1 Publication
Modified residuei635 – 6351Phosphoserine1 Publication
Modified residuei638 – 6381Phosphoserine1 Publication
Modified residuei647 – 6471Phosphoserine1 Publication
Modified residuei677 – 6771Phosphoserine2 Publications
Modified residuei680 – 6801Phosphoserine1 Publication
Modified residuei683 – 6831Phosphoserine1 Publication
Modified residuei769 – 7691Phosphoserine1 Publication
Modified residuei783 – 7831Phosphoserine1 Publication

Post-translational modificationi

Autophosphorylated at Thr-226 and Ser-390. The phosphorylation state may play a role in the induction of protein degradation upon starvation. Phosphorylation at Thr-226 within the activation loop is required for protein kinase activity whereas phosphorylation at Ser-34 leads to inhibition of kinase activity. Phosphorylation of Ser-508 and Ser-515 by PKA is required to induce autophagy but not for kinase activity.7 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53104.
PaxDbiP53104.
PRIDEiP53104.

Expressioni

Gene expression databases

GenevestigatoriP53104.

Interactioni

Subunit structurei

Homodimer. Dimerization requires the presence of ATG13. Forms a ternary complex with ATG13 and ATG17. Interacts also with ATG11.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ATG11Q125273EBI-2657,EBI-31977
ATG13Q0662813EBI-2657,EBI-36188
ATG8P381823EBI-2657,EBI-2684

Protein-protein interaction databases

BioGridi33074. 375 interactions.
DIPiDIP-1192N.
IntActiP53104. 21 interactions.
MINTiMINT-405956.
STRINGi4932.YGL180W.

Structurei

3D structure databases

ProteinModelPortaliP53104.
SMRiP53104. Positions 17-358, 589-894.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 325302Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni880 – 8867Required for Cvt trafficking

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi429 – 4324LIR

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi542 – 5487Poly-Gln

Domaini

The LIR motif is required for the interaction with ATG8 and for the association of ATG1 with autophagosomes.2 Publications

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000119118.
HOGENOMiHOG000246715.
InParanoidiP53104.
KOiK08269.
OMAiEKLMYDR.
OrthoDBiEOG7CCC0Z.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
IPR022708. Ser/Thr_kinase_C.
[Graphical view]
PfamiPF12063. DUF3543. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53104-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGDIKNKDHT TSVNHNLMAS AGNYTAEKEI GKGSFATVYR GHLTSDKSQH
60 70 80 90 100
VAIKEVSRAK LKNKKLLENL EIEIAILKKI KHPHIVGLID CERTSTDFYL
110 120 130 140 150
IMEYCALGDL TFLLKRRKEL MENHPLLRTV FEKYPPPSEN HNGLHRAFVL
160 170 180 190 200
SYLQQLASAL KFLRSKNLVH RDIKPQNLLL STPLIGYHDS KSFHELGFVG
210 220 230 240 250
IYNLPILKIA DFGFARFLPN TSLAETLCGS PLYMAPEILN YQKYNAKADL
260 270 280 290 300
WSVGTVVFEM CCGTPPFRAS NHLELFKKIK RANDVITFPS YCNIEPELKE
310 320 330 340 350
LICSLLTFDP AQRIGFEEFF ANKVVNEDLS SYELEDDLPE LESKSKGIVE
360 370 380 390 400
SNMFVSEYLS KQPKSPNSNL AGHQSMADNP AELSDALKNS NILTAPAVKT
410 420 430 440 450
DHTQAVDKKA SNNKYHNSLV SDRSFEREYV VVEKKSVEVN SLADEVAQAG
460 470 480 490 500
FNPNPIKHPT STQNQNVLLN EQFSPNNQQY FQNQGENPRL LRATSSSSGG
510 520 530 540 550
SDGSRRPSLV DRRLSISSLN PSNALSRALG IASTRLFGGA NQQQQQQQIT
560 570 580 590 600
SSPPYSQTLL NSQLFHELTE NIILRIDHLQ HPETLKLDNT NIVSILESLA
610 620 630 640 650
AKAFVVYSYA EVKFSQIVPL STTLKGMANF ENRRSMDSNA IAEEQDSDDA
660 670 680 690 700
EEEDETLKKY KEDCLSTKTF GKGRTLSATS QLSATFNKLP RSEMILLCNE
710 720 730 740 750
AIVLYMKALS ILSKSMQVTS NWWYESQEKS CSLRVNVLVQ WLREKFNECL
760 770 780 790 800
EKADFLRLKI NDLRFKHASE VAENQTLEEK GSSEEPVYLE KLLYDRALEI
810 820 830 840 850
SKMAAHMELK GENLYNCELA YATSLWMLET SLDDDDFTNA YGDYPFKTNI
860 870 880 890
HLKSNDVEDK EKYHSVLDEN DRIIIRKYID SIANRLKILR QKMNHQN
Length:897
Mass (Da):101,717
Last modified:October 1, 1996 - v1
Checksum:i7F4C785AA3A7CC46
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X91489 Genomic DNA. Translation: CAA62794.1.
D29991 Genomic DNA. Translation: BAA21481.1.
Z72702 Genomic DNA. Translation: CAA96892.1.
BK006941 Genomic DNA. Translation: DAA07934.1.
PIRiS61137.
RefSeqiNP_011335.1. NM_001181045.1.

Genome annotation databases

EnsemblFungiiYGL180W; YGL180W; YGL180W.
GeneIDi852695.
KEGGisce:YGL180W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X91489 Genomic DNA. Translation: CAA62794.1 .
D29991 Genomic DNA. Translation: BAA21481.1 .
Z72702 Genomic DNA. Translation: CAA96892.1 .
BK006941 Genomic DNA. Translation: DAA07934.1 .
PIRi S61137.
RefSeqi NP_011335.1. NM_001181045.1.

3D structure databases

ProteinModelPortali P53104.
SMRi P53104. Positions 17-358, 589-894.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33074. 375 interactions.
DIPi DIP-1192N.
IntActi P53104. 21 interactions.
MINTi MINT-405956.
STRINGi 4932.YGL180W.

Protein family/group databases

TCDBi 9.A.15.1.1. the autophagy-related phagophore-formation transporter (apt) family.

Proteomic databases

MaxQBi P53104.
PaxDbi P53104.
PRIDEi P53104.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGL180W ; YGL180W ; YGL180W .
GeneIDi 852695.
KEGGi sce:YGL180W.

Organism-specific databases

CYGDi YGL180w.
SGDi S000003148. ATG1.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00760000119118.
HOGENOMi HOG000246715.
InParanoidi P53104.
KOi K08269.
OMAi EKLMYDR.
OrthoDBi EOG7CCC0Z.

Enzyme and pathway databases

BioCyci YEAST:G3O-30667-MONOMER.
BRENDAi 2.7.11.1. 984.

Miscellaneous databases

NextBioi 972032.
PROi P53104.

Gene expression databases

Genevestigatori P53104.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
IPR022708. Ser/Thr_kinase_C.
[Graphical view ]
Pfami PF12063. DUF3543. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing of a 40.5 kb fragment located on the left arm of chromosome VII from Saccharomyces cerevisiae."
    Coglievina M., Klima R., Bertani I., Delneri D., Zaccaria P., Bruschi C.V.
    Yeast 13:55-64(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. "Apg1p, a novel protein kinase required for the autophagic process in Saccharomyces cerevisiae."
    Matsuura A., Tsukada M., Wada Y., Ohsumi Y.
    Gene 192:245-250(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, PHOSPHORYLATION, MUTAGENESIS OF ASP-211 AND GLU-237.
    Strain: ATCC 26109 / X2180.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae."
    Tsukada M., Ohsumi Y.
    FEBS Lett. 333:169-174(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Genetic and phenotypic overlap between autophagy and the cytoplasm to vacuole protein targeting pathway."
    Harding T.M., Hefner-Gravink A., Thumm M., Klionsky D.J.
    J. Biol. Chem. 271:17621-17624(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "AUT3, a serine/threonine kinase gene, is essential for autophagocytosis in Saccharomyces cerevisiae."
    Straub M., Bredschneider M., Thumm M.
    J. Bacteriol. 179:3875-3883(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  8. "Apg13p and Vac8p are part of a complex of phosphoproteins that are required for cytoplasm to vacuole targeting."
    Scott S.V., Nice D.C. III, Nau J.J., Weisman L.S., Kamada Y., Keizer-Gunnink I., Funakoshi T., Veenhuis M., Ohsumi Y., Klionsky D.J.
    J. Biol. Chem. 275:25840-25849(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  9. "Tor-mediated induction of autophagy via an Apg1 protein kinase complex."
    Kamada Y., Funakoshi T., Shintani T., Nagano K., Ohsumi M., Ohsumi Y.
    J. Cell Biol. 150:1507-1513(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME REGULATION, MUTAGENESIS OF LYS-54, INTERACTION WITH ATG11; ATG13 AND ATG17.
  10. "The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation."
    Suzuki K., Kirisako T., Kamada Y., Mizushima N., Noda T., Ohsumi Y.
    EMBO J. 20:5971-5981(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  11. "Cvt9/Gsa9 functions in sequestering selective cytosolic cargo destined for the vacuole."
    Kim J., Kamada Y., Stromhaug P.E., Guan J., Hefner-Gravink A., Baba M., Scott S.V., Ohsumi Y., Dunn W.A. Jr., Klionsky D.J.
    J. Cell Biol. 153:381-396(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ATG11.
  12. "Antagonistic controls of autophagy and glycogen accumulation by Snf1p, the yeast homolog of AMP-activated protein kinase, and the cyclin-dependent kinase Pho85p."
    Wang Z., Wilson W.A., Fujino M.A., Roach P.J.
    Mol. Cell. Biol. 21:5742-5752(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  13. "Starvation-induced degradation of yeast hexose transporter Hxt7p is dependent on endocytosis, autophagy and the terminal sequences of the permease."
    Krampe S., Boles E.
    FEBS Lett. 513:193-196(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  14. Cited for: NOMENCLATURE.
  15. "Chemical genetic analysis of Apg1 reveals a non-kinase role in the induction of autophagy."
    Abeliovich H., Zhang C., Dunn W.A. Jr., Shokat K.M., Klionsky D.J.
    Mol. Biol. Cell 14:477-490(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF LYS-54; MET-102; ASN-884 AND LEU-886.
  16. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  17. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  18. "The Atg1-Atg13 complex regulates Atg9 and Atg23 retrieval transport from the pre-autophagosomal structure."
    Reggiori F., Tucker K.A., Stromhaug P.E., Klionsky D.J.
    Dev. Cell 6:79-90(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  19. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-677, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  20. "Overexpression of autophagy-related genes inhibits yeast filamentous growth."
    Ma J., Jin R., Dobry C.J., Lawson S.K., Kumar A.
    Autophagy 3:604-609(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  21. "Hierarchy of Atg proteins in pre-autophagosomal structure organization."
    Suzuki K., Kubota Y., Sekito T., Ohsumi Y.
    Genes Cells 12:209-218(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  22. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  23. "Protein kinase A and Sch9 cooperatively regulate induction of autophagy in Saccharomyces cerevisiae."
    Yorimitsu T., Zaman S., Broach J.R., Klionsky D.J.
    Mol. Biol. Cell 18:4180-4189(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-508 AND SER-515 BY PKA, MUTAGENESIS OF SER-508 AND SER-515, FUNCTION.
  24. "Localization of autophagy-related proteins in yeast using a versatile plasmid-based resource of fluorescent protein fusions."
    Ma J., Bharucha N., Dobry C.J., Frisch R.L., Lawson S., Kumar A.
    Autophagy 4:792-800(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  25. "Mitophagy in yeast occurs through a selective mechanism."
    Kanki T., Klionsky D.J.
    J. Biol. Chem. 283:32386-32393(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  26. "In vivo reconstitution of autophagy in Saccharomyces cerevisiae."
    Cao Y., Cheong H., Song H., Klionsky D.J.
    J. Cell Biol. 182:703-713(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  27. "The Atg1 kinase complex is involved in the regulation of protein recruitment to initiate sequestering vesicle formation for nonspecific autophagy in Saccharomyces cerevisiae."
    Cheong H., Nair U., Geng J., Klionsky D.J.
    Mol. Biol. Cell 19:668-681(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ATG13 AND ATG17.
  28. "Piecemeal microautophagy of the nucleus requires the core macroautophagy genes."
    Krick R., Muehe Y., Prick T., Bremer S., Schlotterhose P., Eskelinen E.L., Millen J., Goldfarb D.S., Thumm M.
    Mol. Biol. Cell 19:4492-4505(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  29. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-390, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  30. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-635; SER-638 AND SER-647, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  31. "Autophosphorylation within the Atg1 activation loop is required for both kinase activity and the induction of autophagy in Saccharomyces cerevisiae."
    Yeh Y.Y., Wrasman K., Herman P.K.
    Genetics 185:871-882(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT THR-226 BY AUTOCATALYSIS, MUTAGENESIS OF THR-226, ENZYME REGULATION, FUNCTION.
  32. "An Atg9-containing compartment that functions in the early steps of autophagosome biogenesis."
    Mari M., Griffith J., Rieter E., Krishnappa L., Klionsky D.J., Reggiori F.
    J. Cell Biol. 190:1005-1022(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  33. "The identification and analysis of phosphorylation sites on the Atg1 protein kinase."
    Yeh Y.Y., Shah K.H., Chou C.C., Hsiao H.H., Wrasman K.M., Stephan J.S., Stamatakos D., Khoo K.H., Herman P.K.
    Autophagy 7:716-726(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-34; THR-129; SER-304; SER-365; SER-390; SER-515; SER-533; SER-551; SER-552; THR-590; SER-621; SER-677; SER-680; SER-683; SER-769 AND SER-783, MUTAGENESIS OF SER-34; SER-551; SER-552; SER-621; SER-677; SER-680; SER-683; SER-769 AND SER-783, FUNCTION.
  34. "Bidirectional regulation between TORC1 and autophagy in Saccharomyces cerevisiae."
    Shin C.S., Huh W.K.
    Autophagy 7:854-862(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  35. "A new autophagy-related checkpoint in the degradation of an ERAD-M target."
    Kario E., Amar N., Elazar Z., Navon A.
    J. Biol. Chem. 286:11479-11491(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  36. "An Atg13 protein-mediated self-association of the Atg1 protein kinase is important for the induction of autophagy."
    Yeh Y.Y., Shah K.H., Herman P.K.
    J. Biol. Chem. 286:28931-28939(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, INTERACTION WITH ATG13, FUNCTION.
  37. "Two MAPK-signaling pathways are required for mitophagy in Saccharomyces cerevisiae."
    Mao K., Wang K., Zhao M., Xu T., Klionsky D.J.
    J. Cell Biol. 193:755-767(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  38. Cited for: FUNCTION.
  39. Cited for: INTERACTION WITH ATG13 AND ATG8, DOMAIN, FUNCTION, SUBCELLULAR LOCATION.
  40. "Aggregate clearance of alpha-synuclein in Saccharomyces cerevisiae depends more on autophagosome and vacuole function than on the proteasome."
    Petroi D., Popova B., Taheri-Talesh N., Irniger S., Shahpasandzadeh H., Zweckstetter M., Outeiro T.F., Braus G.H.
    J. Biol. Chem. 287:27567-27579(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  41. "The autophagy-related protein kinase Atg1 interacts with the ubiquitin-like protein Atg8 via the Atg8 family interacting motif to facilitate autophagosome formation."
    Nakatogawa H., Ohbayashi S., Sakoh-Nakatogawa M., Kakuta S., Suzuki S.W., Kirisako H., Kondo-Kakuta C., Noda N.N., Yamamoto H., Ohsumi Y.
    J. Biol. Chem. 287:28503-28507(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ATG8, SUBCELLULAR LOCATION, DOMAIN, MUTAGENESIS OF TYR-429 AND VAL-432.
  42. "Phosphatidylinositol 4-kinases are required for autophagic membrane trafficking."
    Wang K., Yang Z., Liu X., Mao K., Nair U., Klionsky D.J.
    J. Biol. Chem. 287:37964-37972(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  43. "A late form of nucleophagy in Saccharomyces cerevisiae."
    Mijaljica D., Prescott M., Devenish R.J.
    PLoS ONE 7:E40013-E40013(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  44. "Increased levels of reduced cytochrome b and mitophagy components are required to trigger nonspecific autophagy following induced mitochondrial dysfunction."
    Deffieu M., Bhatia-Kissova I., Salin B., Klionsky D.J., Pinson B., Manon S., Camougrand N.
    J. Cell Sci. 126:415-426(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  45. "Fine mapping of autophagy-related proteins during autophagosome formation in Saccharomyces cerevisiae."
    Suzuki K., Akioka M., Kondo-Kakuta C., Yamamoto H., Ohsumi Y.
    J. Cell Sci. 126:2534-2544(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, FUNCTION, MUTAGENESIS OF LYS-54 AND ASP-211.
  46. "The role of autophagy in genome stability through suppression of abnormal mitosis under starvation."
    Matsui A., Kamada Y., Matsuura A.
    PLoS Genet. 9:E1003245-E1003245(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  47. "Atg1 kinase organizes autophagosome formation by phosphorylating Atg9."
    Papinski D., Kraft C.
    Autophagy 10:1338-1340(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN PHOSPHORYLATION OF ATG9, CATALYTIC ACTIVITY.

Entry informationi

Entry nameiATG1_YEAST
AccessioniPrimary (citable) accession number: P53104
Secondary accession number(s): D6VTX3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1070 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3