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P53099 (TPN1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vitamin B6 transporter TPN1
Alternative name(s):
Transport of pyridoxine protein 1
Gene names
Name:TPN1
Ordered Locus Names:YGL186C
ORF Names:G1370
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length579 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Thiamine-regulated, high affinity import carrier of pyridoxine, pyridoxal and pyridoxamine. Ref.1

Subcellular location

Membrane; Multi-pass membrane protein Ref.1.

Sequence similarities

Belongs to the purine-cytosine permease family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 579579Vitamin B6 transporter TPN1
PRO_0000197929

Regions

Topological domain1 – 9898Cytoplasmic Potential
Transmembrane99 – 11921Helical; Potential
Topological domain120 – 1223Extracellular Potential
Transmembrane123 – 14321Helical; Potential
Topological domain144 – 15714Cytoplasmic Potential
Transmembrane158 – 17821Helical; Potential
Topological domain179 – 19820Extracellular Potential
Transmembrane199 – 21921Helical; Potential
Topological domain220 – 2212Cytoplasmic Potential
Transmembrane222 – 24221Helical; Potential
Topological domain243 – 27432Extracellular Potential
Transmembrane275 – 29521Helical; Potential
Topological domain296 – 3027Cytoplasmic Potential
Transmembrane303 – 32321Helical; Potential
Topological domain324 – 36239Extracellular Potential
Transmembrane363 – 38321Helical; Potential
Topological domain384 – 39411Cytoplasmic Potential
Transmembrane395 – 41521Helical; Potential
Topological domain416 – 4216Extracellular Potential
Transmembrane422 – 44221Helical; Potential
Topological domain443 – 51977Cytoplasmic Potential
Transmembrane520 – 54021Helical; Potential
Topological domain541 – 5455Extracellular Potential
Transmembrane546 – 56621Helical; Potential
Topological domain567 – 57913Cytoplasmic Potential

Sequences

Sequence LengthMass (Da)Tools
P53099 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 26686199D1FD8522

FASTA57964,546
        10         20         30         40         50         60 
MNRDNMDTTK RKEDHTKHTT DVIEFYEEGT AASSLNIATE KANSSPSILR RIINRAAWLS 

        70         80         90        100        110        120 
KKVDAMGVES TGIQRISPYE RGTSKKQFLH VAGLWLSATG GLSSMSSFLL GPLLFGLSFR 

       130        140        150        160        170        180 
ESVASSLISV TIGCLIAAYC SIMGPQSGCR QMVTARYLFG WWFVKLVALA SIIGVMGWSV 

       190        200        210        220        230        240 
VNSVVGGEML AAISNDKVPL WVGIVIVTVC SFLVAIFGIK QVIKVETYLS VPVLTAFLLL 

       250        260        270        280        290        300 
YISSSDKYSF VNAYVSKGNL DSSTRKGNWM SFFSLCYSIT ATWGSITADY YILFPEDTPY 

       310        320        330        340        350        360 
IQIFCLTFFG TFLPTCFVGI LGLLLASVAM SYKPWSVEYD SHGMGGLLWA GFQRWNGFGK 

       370        380        390        400        410        420 
FCVVVLVFSL VSNNIINTYS AAFSIQLSSV FCAKIPRWFW SIVCTIICLV CALIGRNHFS 

       430        440        450        460        470        480 
TILGNFLPMI GYWISMYFIL LFEENLVFRR FFLHLYTKEF PTVTGEINGP ELVGSSKEVE 

       490        500        510        520        530        540 
KDAVTNIHLL KRKHKVTKHR YNWDKWEDYE VLTHGYAATF AFIVGVAGVV VGMAQAYWIG 

       550        560        570 
PIAAKFGEYG GDVAMWLSMA FSGVVYPPCR YLELRKFGR

« Hide

References

« Hide 'large scale' references
[1]"Tpn1p, the plasma membrane vitamin B6 transporter of Saccharomyces cerevisiae."
Stolz J., Vielreicher M.
J. Biol. Chem. 278:18990-18996(2003) [PubMed: 12649274] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
Strain: ATCC 208353 / W303-1A.
[2]"Sequencing of a 40.5 kb fragment located on the left arm of chromosome VII from Saccharomyces cerevisiae."
Coglievina M., Klima R., Bertani I., Delneri D., Zaccaria P., Bruschi C.V.
Yeast 13:55-64(1997) [PubMed: 9046087] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed: 9169869] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X91489 Genomic DNA. Translation: CAA62788.1.
Z72708 Genomic DNA. Translation: CAA96898.1.
BK006941 Genomic DNA. Translation: DAA07929.1.
PIRS61131.
RefSeqNP_011329.1. NM_001181051.1.

3D structure databases

ProteinModelPortalP53099.
ModBaseSearch...

Protein-protein interaction databases

IntActP53099. 1 interaction.
STRINGP53099.

Protein family/group databases

TCDB2.A.39.2.2. nucleobase:cation symporter-1 (NCS1) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGL186C; YGL186C; YGL186C.
GeneID852689.
KEGGsce:YGL186C.
NMPDRfig|4932.3.peg.2433.

Organism-specific databases

CYGDYGL186c.
SGDS000003154. TPN1.

Phylogenomic databases

eggNOGfuNOG05349.
GeneTreeEFGT00050000001818.
HOGENOMHBG395679.
OMAFGWWFVK.
OrthoDBEOG44J5S8.

Gene expression databases

ArrayExpressP53099.
GenevestigatorP53099.
GermOnlineYGL186C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR001248. Cyt_pur_permease.
IPR012681. NCS1.
[Graphical view]
PfamPF02133. Transp_cyt_pur. 1 hit.
[Graphical view]
TIGRFAMsTIGR00800. Ncs1. 1 hit.
ProtoNetSearch...

Other

NextBio972017.

Entry information

Entry nameTPN1_YEAST
AccessionPrimary (citable) accession number: P53099
Secondary accession number(s): D6VTW8
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: December 14, 2011
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents