Reviewed, UniProtKB/Swiss-Prot P53099 (TPN1_YEAST)

Names and origin

Protein names

Recommended name:
    Vitamin B6 transporter TPN1
Alternative name(s):
    Transport of pyridoxine protein 1

Gene names

Name:	TPN1
Ordered Locus Names:	YGL186C
ORF Names:	G1370

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	579 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Thiamine-regulated, high affinity import carrier of pyridoxine, pyridoxal and pyridoxamine. Ref.1
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the purine-cytosine permease family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane
Domain	Transmembrane
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleobase, nucleoside, nucleotide and nucleic acid transport Inferred from electronic annotation. Source: InterPro transmembrane transport Inferred from electronic annotation. Source: InterPro vitamin transport Ref.1 Inferred from direct assay. Source: SGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Ref.1 Inferred from direct assay. Source: SGD
Molecular function	nucleobase transmembrane transporter activity Inferred from electronic annotation. Source: InterPro vitamin transporter activity Ref.1 Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 579	579	Vitamin B6 transporter TPN1		PRO_0000197929
Regions
	Topological domain	1 – 98	98	Cytoplasmic Potential
	Transmembrane	99 – 119	21	Potential
	Topological domain	120 – 122	3	Extracellular Potential
	Transmembrane	123 – 143	21	Potential
	Topological domain	144 – 157	14	Cytoplasmic Potential
	Transmembrane	158 – 178	21	Potential
	Topological domain	179 – 198	20	Extracellular Potential
	Transmembrane	199 – 219	21	Potential
	Topological domain	220 – 221	2	Cytoplasmic Potential
	Transmembrane	222 – 242	21	Potential
	Topological domain	243 – 274	32	Extracellular Potential
	Transmembrane	275 – 295	21	Potential
	Topological domain	296 – 302	7	Cytoplasmic Potential
	Transmembrane	303 – 323	21	Potential
	Topological domain	324 – 362	39	Extracellular Potential
	Transmembrane	363 – 383	21	Potential
	Topological domain	384 – 394	11	Cytoplasmic Potential
	Transmembrane	395 – 415	21	Potential
	Topological domain	416 – 421	6	Extracellular Potential
	Transmembrane	422 – 442	21	Potential
	Topological domain	443 – 519	77	Cytoplasmic Potential
	Transmembrane	520 – 540	21	Potential
	Topological domain	541 – 545	5	Extracellular Potential
	Transmembrane	546 – 566	21	Potential
	Topological domain	567 – 579	13	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P53099-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 26686199D1FD8522	FASTA	579	64,546
	10 20 30 40 50 60 MNRDNMDTTK RKEDHTKHTT DVIEFYEEGT AASSLNIATE KANSSPSILR RIINRAAWLS 70 80 90 100 110 120 KKVDAMGVES TGIQRISPYE RGTSKKQFLH VAGLWLSATG GLSSMSSFLL GPLLFGLSFR 130 140 150 160 170 180 ESVASSLISV TIGCLIAAYC SIMGPQSGCR QMVTARYLFG WWFVKLVALA SIIGVMGWSV 190 200 210 220 230 240 VNSVVGGEML AAISNDKVPL WVGIVIVTVC SFLVAIFGIK QVIKVETYLS VPVLTAFLLL 250 260 270 280 290 300 YISSSDKYSF VNAYVSKGNL DSSTRKGNWM SFFSLCYSIT ATWGSITADY YILFPEDTPY 310 320 330 340 350 360 IQIFCLTFFG TFLPTCFVGI LGLLLASVAM SYKPWSVEYD SHGMGGLLWA GFQRWNGFGK 370 380 390 400 410 420 FCVVVLVFSL VSNNIINTYS AAFSIQLSSV FCAKIPRWFW SIVCTIICLV CALIGRNHFS 430 440 450 460 470 480 TILGNFLPMI GYWISMYFIL LFEENLVFRR FFLHLYTKEF PTVTGEINGP ELVGSSKEVE 490 500 510 520 530 540 KDAVTNIHLL KRKHKVTKHR YNWDKWEDYE VLTHGYAATF AFIVGVAGVV VGMAQAYWIG 550 560 570 PIAAKFGEYG GDVAMWLSMA FSGVVYPPCR YLELRKFGR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Tpn1p, the plasma membrane vitamin B6 transporter of Saccharomyces cerevisiae." Stolz J., Vielreicher M. J. Biol. Chem. 278:18990-18996(2003) [PubMed: 12649274] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION. Strain: ATCC 208353 / W303-1A.
[2]	"Sequencing of a 40.5 kb fragment located on the left arm of chromosome VII from Saccharomyces cerevisiae." Coglievina M., Klima R., Bertani I., Delneri D., Zaccaria P., Bruschi C.V. Yeast 13:55-64(1997) [PubMed: 9046087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X91489 Genomic DNA. Translation: CAA62788.1. Z72708 Genomic DNA. Translation: CAA96898.1.
PIR	S61131.
RefSeq	NP_011329.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P53099.
Protein family/group databases
TCDB	2.A.39.2.2. nucleobase:cation symporter-1 (NCS1) family.
Genome annotation databases
Ensembl	YGL186C; YGL186C; YGL186C; Saccharomyces cerevisiae. [Genome view]
GeneID	852689.
KEGG	sce:YGL186C.
NMPDR	fig|4932.3.peg.2433.
Organism-specific databases
CYGD	YGL186c.
SGD	S000003154. TPN1.
Phylogenomic databases
HOGENOM	P53099.
OMA	FGWWFVK
OrthoDB	EOG9XWHF2
Gene expression databases
ArrayExpress	P53099.
Genevestigator	P53099.
GermOnline	YGL186C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001248. Cyt_pur_permease. IPR012681. NCS1. [Graphical view]
Pfam	PF02133. Transp_cyt_pur. 1 hit. [Graphical view]
TIGRFAMs	TIGR00800. ncs1. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	972017.

Entry information

Entry name

TPN1_YEAST

Accession

Primary (citable) accession number: P53099

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 24, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)