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P53096 (HOS2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable histone deacetylase HOS2

EC=3.5.1.98
Gene names
Name:HOS2
Ordered Locus Names:YGL194C
ORF Names:G1330
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length452 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). It is apparently involved in transcriptional activation. Ref.6

Catalytic activity

Hydrolysis of an N(6)-acetyl-lysine residue of a histone to yield a deacetylated histone.

Subunit structure

Identified in a Set3C complex with SET3, HST1, SNT1, SIF2, CPR1 and HOS4/YIL112W. Ref.5

Subcellular location

Nucleus.

Miscellaneous

Present with 4890 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the histone deacetylase family. HD type 1 subfamily.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Molecular functionActivator
Chromatin regulator
Hydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processchromatin organization

Inferred from sequence or structural similarity Ref.4. Source: SGD

histone deacetylation

Inferred from direct assay Ref.5. Source: SGD

negative regulation of meiosis

Inferred from mutant phenotype Ref.5. Source: SGD

positive regulation of stress-activated MAPK cascade

Inferred from mutant phenotype PubMed 18487345. Source: SGD

regulation of transcription, DNA-templated

Inferred from mutant phenotype PubMed 19379692. Source: SGD

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentSet3 complex

Inferred from direct assay Ref.5. Source: SGD

   Molecular_functionNAD-dependent histone deacetylase activity

Inferred from direct assay Ref.5. Source: SGD

NAD-dependent histone deacetylase activity (H3-K14 specific)

Inferred from electronic annotation. Source: UniProtKB-EC

NAD-dependent histone deacetylase activity (H3-K18 specific)

Inferred from electronic annotation. Source: UniProtKB-EC

NAD-dependent histone deacetylase activity (H3-K9 specific)

Inferred from electronic annotation. Source: UniProtKB-EC

NAD-dependent histone deacetylase activity (H4-K16 specific)

Inferred from electronic annotation. Source: UniProtKB-EC

NAD-independent histone deacetylase activity

Inferred from direct assay Ref.5. Source: SGD

protein binding

Inferred from physical interaction PubMed 11069890PubMed 14525981PubMed 19379692. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CYC8P149224EBI-8475,EBI-18215
HHT2P618302EBI-8475,EBI-8098

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 452452Probable histone deacetylase HOS2
PRO_0000114723

Regions

Region26 – 340315Histone deacetylase

Sites

Active site1971 By similarity

Experimental info

Mutagenesis196 – 1972HH → AA: Leads to hyperacetylation. Ref.6
Sequence conflict352 – 36413PFRDS…DYSLY → HSGTHSGRIIHFI Ref.1

Sequences

Sequence LengthMass (Da)Tools
P53096 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 024E8AEA0A445A08

FASTA45251,455
        10         20         30         40         50         60 
MSGTFSYDVK TKENEPLFEF NSAYSPRVSY HFNSKVSHYH YGVKHPMKPF RLMLTDHLVS 

        70         80         90        100        110        120 
SYGLHKIMDL YETRSATRDE LLQFHSEDYV NFLSKVSPEN ANKLPRGTLE NFNIGDDCPI 

       130        140        150        160        170        180 
FQNLYDYTTL YTGASLDATR KLINNQSDIA INWSGGLHHA KKNSPSGFCY VNDIVLSILN 

       190        200        210        220        230        240 
LLRYHPRILY IDIDLHHGDG VQEAFYTTDR VFTLSFHKYN GEFFPGTGDL TEIGCDKGKH 

       250        260        270        280        290        300 
FALNVPLEDG IDDDSYINLF KSIVDPLIMT FKPTLIVQQC GADSLGHDRL GCFNLNIKAH 

       310        320        330        340        350        360 
GECVKFVKSF GLPMLVVGGG GYTPRNVSRL WTYETGILND VLLPEDIPED IPFRDSFGPD 

       370        380        390        400        410        420 
YSLYPMLDDL YENKNSKKLL EDIRIRCLEN IRYLQGAPSV RMDAECIPTQ DISALTEEED 

       430        440        450 
KIIQEMNEET EADSSNRLEE MEKENSGLIA FS 

« Hide

References

« Hide 'large scale' references
[1]"Sequencing of a 40.5 kb fragment located on the left arm of chromosome VII from Saccharomyces cerevisiae."
Coglievina M., Klima R., Bertani I., Delneri D., Zaccaria P., Bruschi C.V.
Yeast 13:55-64(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"HDA1 and RPD3 are members of distinct yeast histone deacetylase complexes that regulate silencing and transcription."
Rundlett S.E., Carmen A.A., Kobayashi R., Bavykin S., Turner B.M., Grunstein M.
Proc. Natl. Acad. Sci. U.S.A. 93:14503-14508(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE NAME.
[5]"The S. cerevisiae SET3 complex includes two histone deacetylases, Hos2 and Hst1, and is a meiotic-specific repressor of the sporulation gene program."
Pijnappel W.W.M.P., Schaft D., Roguev A., Shevchenko A., Tekotte H., Wilm M., Rigaut G., Seraphin B., Aasland R., Stewart A.F.
Genes Dev. 15:2991-3004(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN A COMPLEX WITH SET3; HST1; SNT1; SIF2; CPR1 AND YIL112W.
[6]"Requirement of Hos2 histone deacetylase for gene activity in yeast."
Wang A., Kurdistani S.K., Grunstein M.
Science 298:1412-1414(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF 195-HIS-HIS-196.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X91837 Genomic DNA. Translation: CAA62950.1.
Z72716 Genomic DNA. Translation: CAA96906.1.
BK006941 Genomic DNA. Translation: DAA07921.1.
PIRS64211.
RefSeqNP_011321.1. NM_001181059.1.

3D structure databases

ProteinModelPortalP53096.
SMRP53096. Positions 28-394.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33063. 236 interactions.
DIPDIP-6828N.
IntActP53096. 37 interactions.
MINTMINT-629670.
STRING4932.YGL194C.

Proteomic databases

MaxQBP53096.
PaxDbP53096.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGL194C; YGL194C; YGL194C.
GeneID852681.
KEGGsce:YGL194C.

Organism-specific databases

CYGDYGL194c.
SGDS000003162. HOS2.

Phylogenomic databases

eggNOGCOG0123.
GeneTreeENSGT00390000003411.
HOGENOMHOG000225180.
KOK11483.
OMADKQIHHI.
OrthoDBEOG78M0B2.

Enzyme and pathway databases

BioCycYEAST:G3O-30675-MONOMER.

Gene expression databases

GenevestigatorP53096.

Family and domain databases

Gene3D3.40.800.20. 1 hit.
InterProIPR000286. His_deacetylse.
IPR003084. His_deacetylse_1.
IPR023801. His_deacetylse_dom.
[Graphical view]
PANTHERPTHR10625. PTHR10625. 1 hit.
PfamPF00850. Hist_deacetyl. 1 hit.
[Graphical view]
PIRSFPIRSF037913. His_deacetylse_1. 1 hit.
PRINTSPR01270. HDASUPER.
PR01271. HISDACETLASE.
ProtoNetSearch...

Other

NextBio971996.

Entry information

Entry nameHOS2_YEAST
AccessionPrimary (citable) accession number: P53096
Secondary accession number(s): D6VTW0
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families