P53095 (DSD1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-serine dehydratase
EC=4.3.1.18

Alternative name(s):
D-serine deaminase
Short name=DSD

Gene names

Name:	DSD1
Ordered Locus Names:	YGL196W
ORF Names:	G1315

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Converts specifically D-serine to pyruvate and ammonia. May play a role in D-serine detoxification. Ref.6 Ref.7
Catalytic activity	D-serine = pyruvate + NH3. Ref.6 Ref.7
Cofactor	Pyridoxal phosphate and zinc. Ref.7
Enzyme regulation	Sodium cyanoborohydride, N-ethylmaleimide, hydroxylamine, phenyhydrazin and EDTA are inhibitors of the catalytic activity. Ref.7
Subunit structure	Homodimer. Ref.7
Sequence similarities	Belongs to the DSD1 family.
Biophysicochemical properties	Kinetic parameters: KM=0.39 mM for D-serine Ref.7 KM=0.13 mM for D-threonine KM=1.45 mM for beta-Cl-D-alanine pH dependence: Optimum pH is 8.0.
Sequence caution	The sequence CAA62948.1 differs from that shown. Reason: Frameshift at positions 74, 138, 177, 370 and 387. The sequence CAA96908.1 differs from that shown. Reason: Frameshift at positions 74, 138, 177 and 387.

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	D-serine metabolic process Inferred from mutant phenotype Ref.7. Source: SGD
Molecular function	D-serine ammonia-lyase activity Inferred from direct assay Ref.6Ref.7. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	D-serine dehydratase		PRO_0000202722
Experimental info
	Sequence conflict	125	1	S → R in CAA62948. Ref.1
	Sequence conflict	125	1	S → R in CAA96908. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P53095 [UniParc]. Last modified May 16, 2006. Version 2. Checksum: 0A068BA701FDA879	FASTA	428	47,828
	10 20 30 40 50 60 MSDVLSQYKG CSVRDLPTPN FVINEEKFDK NCTTMLNNVE KLSQECGVPI KFRAHVKTHK 70 80 90 100 110 120 TAKGTLKQLG HGLPLAKRTT RAILVSTLKE AEELLNYQDR QCSDYIDDIT YSLPCCVPEF 130 140 150 160 170 180 IPLLSNLSRR VNNFQVFVDN IEHLENLKNF GRPASGKKWS VFIKVDMGTK RAGLAFDSPE 190 200 210 220 230 240 FLSLLKKLTS SEIKEVIEPY GFYAHAGHSY SSTSINDTQN LLMEEVKAVN SAAKVLCSVD 250 260 270 280 290 300 PQFDPSKLTL SVGATPTSNS LKLDNKSTLV KFITTQLVST LEIHCGNYCM YDLQQVATGC 310 320 330 340 350 360 VQDHELSGFV LGTVLSSYPS RGELLSNTGV MCLTREASSI KGFGICADLE HVLKSESFSR 370 380 390 400 410 420 EWYVARVSQE HGILRPIRNW NETTPLKLGS KIAVLPQHAC ITMGQFPYYF VVNSEGIVND VWLPFQKW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing of a 40.5 kb fragment located on the left arm of chromosome VII from Saccharomyces cerevisiae." Coglievina M., Klima R., Bertani I., Delneri D., Zaccaria P., Bruschi C.V. Yeast 13:55-64(1997) [PubMed: 9046087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 223-387. Strain: ATCC 204508 / S288c.
[5]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed: 12748633] [Abstract] Cited for: IDENTIFICATION OF FRAMESHIFTS.
[6]	"Enzymatic assay of D-serine using D-serine dehydratase from Saccharomyces cerevisiae." Ito T., Takahashi K., Naka T., Hemmi H., Yoshimura T. Anal. Biochem. 371:167-172(2007) [PubMed: 17869212] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY.
[7]	"A novel zinc-dependent D-serine dehydratase from Saccharomyces cerevisiae." Ito T., Hemmi H., Kataoka K., Mukai Y., Yoshimura T. Biochem. J. 409:399-406(2008) [PubMed: 17937657] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, HOMODIMERIZATION, COFACTOR, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X91837 Genomic DNA. Translation: CAA62948.1. Frameshift. Z72718 Genomic DNA. Translation: CAA96908.1. Frameshift. AY558389 Genomic DNA. Translation: AAS56715.1. BK006941 Genomic DNA. Translation: DAA07918.1.
PIR	S64213.
RefSeq	NP_011319.2. NM_001181061.1.
3D structure databases
ProteinModelPortal	P53095.
SMR	P53095. Positions 8-428.
ModBase	Search...
Protein-protein interaction databases
STRING	P53095.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL196W; YGL196W; YGL196W.
GeneID	852679.
KEGG	sce:YGL196W.
Organism-specific databases
CYGD	YGL196w.
SGD	S000003164. DSD1.
Phylogenomic databases
eggNOG	fuNOG07683.
GeneTree	EFGT00050000001011.
HOGENOM	HBG324429.
OMA	LPCPVVS.
OrthoDB	EOG4W9NCV.
Gene expression databases
ArrayExpress	P53095.
Genevestigator	P53095.
GermOnline	YGL196W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001608. Ala_racemase_N. [Graphical view]
Pfam	PF01168. Ala_racemase_N. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971990.

Entry information

Entry name

DSD1_YEAST

Accession

Primary (citable) accession number: P53095
Secondary accession number(s): D6VTV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	May 16, 2006
Last modified:	December 14, 2011
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program