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P53094

- MDS3_YEAST

UniProt

P53094 - MDS3_YEAST

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Protein
Negative regulator of sporulation MDS3
Gene
MDS3, YGL197W, G1307
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Negatively regulates early sporulation-specific genes. Seems to exert its function by positively regulating the Ras/cAMP pathway. Required for growth under alkaline conditions. Acts synergetically with PMD1.2 Publications

GO - Biological processi

  1. TOR signaling Source: SGD
  2. meiotic nuclear division Source: UniProtKB-KW
  3. negative regulation of sporulation resulting in formation of a cellular spore Source: SGD
  4. pseudohyphal growth Source: SGD
  5. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Meiosis, Sporulation

Enzyme and pathway databases

BioCyciYEAST:G3O-30678-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Negative regulator of sporulation MDS3
Alternative name(s):
MCK1 dosage suppressor 3
Gene namesi
Name:MDS3
Ordered Locus Names:YGL197W
ORF Names:G1307
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGL197w.
SGDiS000003165. MDS3.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14871487Negative regulator of sporulation MDS3
PRO_0000119137Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei472 – 4721Phosphoserine1 Publication
Modified residuei475 – 4751Phosphoserine1 Publication
Modified residuei621 – 6211Phosphoserine3 Publications
Modified residuei639 – 6391Phosphoserine1 Publication
Modified residuei693 – 6931Phosphoserine3 Publications
Modified residuei698 – 6981Phosphoserine1 Publication
Modified residuei744 – 7441Phosphoserine1 Publication
Modified residuei747 – 7471Phosphoserine1 Publication
Modified residuei756 – 7561Phosphoserine1 Publication
Modified residuei757 – 7571Phosphoserine1 Publication
Modified residuei781 – 7811Phosphoserine2 Publications
Modified residuei785 – 7851Phosphothreonine1 Publication
Modified residuei787 – 7871Phosphoserine1 Publication
Modified residuei900 – 9001Phosphoserine1 Publication
Modified residuei904 – 9041Phosphoserine1 Publication
Modified residuei930 – 9301Phosphothreonine1 Publication
Modified residuei1187 – 11871Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53094.
PaxDbiP53094.

Expressioni

Gene expression databases

GenevestigatoriP53094.

Interactioni

Protein-protein interaction databases

BioGridi33060. 77 interactions.
DIPiDIP-6381N.
IntActiP53094. 24 interactions.
MINTiMINT-598198.
STRINGi4932.YGL197W.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati171 – 22656Kelch 1
Add
BLAST
Repeati234 – 28754Kelch 2
Add
BLAST
Repeati371 – 41949Kelch 3
Add
BLAST

Sequence similaritiesi

Contains 3 Kelch repeats.

Keywords - Domaini

Kelch repeat, Repeat

Phylogenomic databases

eggNOGiNOG41848.
GeneTreeiENSGT00530000066388.
HOGENOMiHOG000113513.
OMAiHDYGSHR.
OrthoDBiEOG7HQNHW.

Family and domain databases

Gene3Di2.120.10.80. 3 hits.
InterProiIPR015915. Kelch-typ_b-propeller.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53094-1 [UniParc]FASTAAdd to Basket

« Hide

MPLLQPSTCF CYPLKLPPLP LTSDSNEFDE CARKRLTLDY RTGSAVTLTR     50
SNIFVHGGLT IPLNLPVVNS MQLQKELILF FAKEKNNGSS FRNLNEWISK 100
ETFFLDLMSR TWYRVKTSFD QRTEELLKAE SSSAKADNDT NEIRTDIKKG 150
KSLESPLKER LFHSLCYLDG CLYIFGGLTV SPQSGYELIA TNELWKLDLN 200
TKKWSLLSDD PQIARRFNHT MHVKNENNDN RDTKLIIVGG LNNMDQPVKK 250
IDIYNISQNC WQSETIPKQP MEITTNVNGI PLALSKDQNF SILVENNEAN 300
VPALAFYMRS DQIDEYLGKD SSKIKENSPI VALPLLSESQ GIRMPSNPAL 350
PKKLLNVPYE LLAPTGDYFG FNIIIGGFHP NYQSSNFHCF IYDINSGKWS 400
RVATACPDCD INKHRFWRVF VWKSHHQTIL LGTKTDDYYS PSVQRFDHLS 450
TFGLPLVNIF NKTIQLPHHK ISASSLPIPI ENFAKHKDTP LKKVSFTSSA 500
TSQFENYIRY IAPPLEMSSI QSVFPPYAMV LGKDALEIYG KPLSDFEFIT 550
SEGDSIGIPC YLLRKRWGRY FDMLLSQSYT KVCADYETTD TQSTLIKFSP 600
HSSRNSSKAV RQEGRLSSSG SLDNYFEKNF PIFARTSVSE AQNTQPQVAN 650
ADAKAPNTPS TSDEPSSSSS SDLYSTPHYQ RNNDEEDDED PVSPKPVSKS 700
NSIYRPIRKT ESSSTTSSSN GMIFRVPFKE KAAVTSNTEA LLESNLSLQE 750
LSRRRSSLMS IPSGELLRSS ISEAEHQRRA SHPLTSSPLF EDSGTPCGKQ 800
LQQLQQHTIQ NPHNHLSPRR FSRSARSSIS YVSSSSDRRG NSISSRSTSD 850
SFGTPPVLGV LNVPLPPQTR EPNEPPPPCP AMSTGSNTRR SNTLTDYMHS 900
NKASPFSSRR SSHIGRRSST PETENAFSAT PRASLDGQML GKSLKEGSTS 950
QYTQPRMNSF PKANETIQTP TSSNNEWSRQ SVTSNTDSFD SLQSNFALEL 1000
EPLLTPRSLY MPWPTSTVRA FAEFFYTGQV NSKWLLAPVA LDLLVMAKIY 1050
EIPLLYKLIL EVLYSILAKK EESLSLICTS LMETFRTKTL NSYKGDEEKT 1100
NTYLTSNDNY QELLKLKVSL ENIDNGYYDP DLLRKQSRAQ SSSTQESSGS 1150
ANGEKTATGA GSLETSSTNV PTVFAGGPRD SHNSVGSIGF PNSMNIQGSR 1200
RSTSGFSPRV KMKSSLSKEI DPKTFYEEYE PKEGKSFDDN DDQQTNIGSF 1250
NLHLFDMNYG SISSSSTNSI SSSDLEEKEE QEQLQDLLEI EREDSAEILD 1300
ARFRNKEDDK VTKDISNDKK RNYLPHEKNN LKAKEGKETR DVREEEEEFD 1350
FGLGMLSLNK IKREAKHVDK VDDSVDPLFK SSAFPQSPIR AYGSTTRTSS 1400
ASGKPFRDNR SFNAFSVLTL ENMASANALP PVDYVIKSIY RTTVLVNDIR 1450
LMVRCMDCIE LSKNLRALKK KTMEDISKLK GISKPSP 1487
Length:1,487
Mass (Da):166,971
Last modified:September 21, 2011 - v2
Checksum:i31073D68ACA4AF61
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti262 – 2643QSE → HPK in CAA62947. 1 Publication
Sequence conflicti262 – 2643QSE → HPK in CAA96909. 1 Publication
Sequence conflicti403 – 4031A → R in CAA62947. 1 Publication
Sequence conflicti403 – 4031A → R in CAA96909. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X91837 Genomic DNA. Translation: CAA62947.1.
Z72719 Genomic DNA. Translation: CAA96909.1.
BK006941 Genomic DNA. Translation: DAA07917.2.
PIRiS62048.
RefSeqiNP_011318.2. NM_001181062.2.

Genome annotation databases

EnsemblFungiiYGL197W; YGL197W; YGL197W.
GeneIDi852678.
KEGGisce:YGL197W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X91837 Genomic DNA. Translation: CAA62947.1 .
Z72719 Genomic DNA. Translation: CAA96909.1 .
BK006941 Genomic DNA. Translation: DAA07917.2 .
PIRi S62048.
RefSeqi NP_011318.2. NM_001181062.2.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33060. 77 interactions.
DIPi DIP-6381N.
IntActi P53094. 24 interactions.
MINTi MINT-598198.
STRINGi 4932.YGL197W.

Proteomic databases

MaxQBi P53094.
PaxDbi P53094.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGL197W ; YGL197W ; YGL197W .
GeneIDi 852678.
KEGGi sce:YGL197W.

Organism-specific databases

CYGDi YGL197w.
SGDi S000003165. MDS3.

Phylogenomic databases

eggNOGi NOG41848.
GeneTreei ENSGT00530000066388.
HOGENOMi HOG000113513.
OMAi HDYGSHR.
OrthoDBi EOG7HQNHW.

Enzyme and pathway databases

BioCyci YEAST:G3O-30678-MONOMER.

Miscellaneous databases

NextBioi 971987.

Gene expression databases

Genevestigatori P53094.

Family and domain databases

Gene3Di 2.120.10.80. 3 hits.
InterProi IPR015915. Kelch-typ_b-propeller.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing of a 40.5 kb fragment located on the left arm of chromosome VII from Saccharomyces cerevisiae."
    Coglievina M., Klima R., Bertani I., Delneri D., Zaccaria P., Bruschi C.V.
    Yeast 13:55-64(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 262-264 AND 403.
    Strain: ATCC 204508 / S288c.
  4. "Identification of a new class of negative regulators affecting sporulation-specific gene expression in yeast."
    Benni M.L., Neigeborn L.
    Genetics 147:1351-1366(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "Candida albicans Mds3p, a conserved regulator of pH responses and virulence identified through insertional mutagenesis."
    Davis D.A., Bruno V.M., Loza L., Filler S.G., Mitchell A.P.
    Genetics 162:1573-1581(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-639, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-621; SER-693 AND SER-781, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-621; SER-693; SER-698 AND SER-904, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "The Ras/cAMP pathway and the CDK-like kinase Ime2 regulate the MAPK Smk1 and spore morphogenesis in Saccharomyces cerevisiae."
    McDonald C.M., Wagner M., Dunham M.J., Shin M.E., Ahmed N.T., Winter E.
    Genetics 181:511-523(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-472; SER-475; SER-621; SER-693; SER-744; SER-747; SER-756; SER-757; SER-781; THR-785; SER-787; SER-900; THR-930 AND SER-1187, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMDS3_YEAST
AccessioniPrimary (citable) accession number: P53094
Secondary accession number(s): D6VTV6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 21, 2011
Last modified: September 3, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 396 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

Similar proteinsi