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P53094

- MDS3_YEAST

UniProt

P53094 - MDS3_YEAST

Protein

Negative regulator of sporulation MDS3

Gene

MDS3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 2 (21 Sep 2011)
      Previous versions | rss
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    Functioni

    Negatively regulates early sporulation-specific genes. Seems to exert its function by positively regulating the Ras/cAMP pathway. Required for growth under alkaline conditions. Acts synergetically with PMD1.2 Publications

    GO - Biological processi

    1. meiotic nuclear division Source: UniProtKB-KW
    2. negative regulation of sporulation resulting in formation of a cellular spore Source: SGD
    3. pseudohyphal growth Source: SGD
    4. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
    5. TOR signaling Source: SGD

    Keywords - Biological processi

    Meiosis, Sporulation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30678-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Negative regulator of sporulation MDS3
    Alternative name(s):
    MCK1 dosage suppressor 3
    Gene namesi
    Name:MDS3
    Ordered Locus Names:YGL197W
    ORF Names:G1307
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGL197w.
    SGDiS000003165. MDS3.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 14871487Negative regulator of sporulation MDS3PRO_0000119137Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei472 – 4721Phosphoserine1 Publication
    Modified residuei475 – 4751Phosphoserine1 Publication
    Modified residuei621 – 6211Phosphoserine3 Publications
    Modified residuei639 – 6391Phosphoserine1 Publication
    Modified residuei693 – 6931Phosphoserine3 Publications
    Modified residuei698 – 6981Phosphoserine1 Publication
    Modified residuei744 – 7441Phosphoserine1 Publication
    Modified residuei747 – 7471Phosphoserine1 Publication
    Modified residuei756 – 7561Phosphoserine1 Publication
    Modified residuei757 – 7571Phosphoserine1 Publication
    Modified residuei781 – 7811Phosphoserine2 Publications
    Modified residuei785 – 7851Phosphothreonine1 Publication
    Modified residuei787 – 7871Phosphoserine1 Publication
    Modified residuei900 – 9001Phosphoserine1 Publication
    Modified residuei904 – 9041Phosphoserine1 Publication
    Modified residuei930 – 9301Phosphothreonine1 Publication
    Modified residuei1187 – 11871Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP53094.
    PaxDbiP53094.

    Expressioni

    Gene expression databases

    GenevestigatoriP53094.

    Interactioni

    Protein-protein interaction databases

    BioGridi33060. 77 interactions.
    DIPiDIP-6381N.
    IntActiP53094. 24 interactions.
    MINTiMINT-598198.
    STRINGi4932.YGL197W.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati171 – 22656Kelch 1Add
    BLAST
    Repeati234 – 28754Kelch 2Add
    BLAST
    Repeati371 – 41949Kelch 3Add
    BLAST

    Sequence similaritiesi

    Contains 3 Kelch repeats.Curated

    Keywords - Domaini

    Kelch repeat, Repeat

    Phylogenomic databases

    eggNOGiNOG41848.
    GeneTreeiENSGT00530000066388.
    HOGENOMiHOG000113513.
    OMAiHDYGSHR.
    OrthoDBiEOG7HQNHW.

    Family and domain databases

    Gene3Di2.120.10.80. 3 hits.
    InterProiIPR015915. Kelch-typ_b-propeller.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53094-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPLLQPSTCF CYPLKLPPLP LTSDSNEFDE CARKRLTLDY RTGSAVTLTR     50
    SNIFVHGGLT IPLNLPVVNS MQLQKELILF FAKEKNNGSS FRNLNEWISK 100
    ETFFLDLMSR TWYRVKTSFD QRTEELLKAE SSSAKADNDT NEIRTDIKKG 150
    KSLESPLKER LFHSLCYLDG CLYIFGGLTV SPQSGYELIA TNELWKLDLN 200
    TKKWSLLSDD PQIARRFNHT MHVKNENNDN RDTKLIIVGG LNNMDQPVKK 250
    IDIYNISQNC WQSETIPKQP MEITTNVNGI PLALSKDQNF SILVENNEAN 300
    VPALAFYMRS DQIDEYLGKD SSKIKENSPI VALPLLSESQ GIRMPSNPAL 350
    PKKLLNVPYE LLAPTGDYFG FNIIIGGFHP NYQSSNFHCF IYDINSGKWS 400
    RVATACPDCD INKHRFWRVF VWKSHHQTIL LGTKTDDYYS PSVQRFDHLS 450
    TFGLPLVNIF NKTIQLPHHK ISASSLPIPI ENFAKHKDTP LKKVSFTSSA 500
    TSQFENYIRY IAPPLEMSSI QSVFPPYAMV LGKDALEIYG KPLSDFEFIT 550
    SEGDSIGIPC YLLRKRWGRY FDMLLSQSYT KVCADYETTD TQSTLIKFSP 600
    HSSRNSSKAV RQEGRLSSSG SLDNYFEKNF PIFARTSVSE AQNTQPQVAN 650
    ADAKAPNTPS TSDEPSSSSS SDLYSTPHYQ RNNDEEDDED PVSPKPVSKS 700
    NSIYRPIRKT ESSSTTSSSN GMIFRVPFKE KAAVTSNTEA LLESNLSLQE 750
    LSRRRSSLMS IPSGELLRSS ISEAEHQRRA SHPLTSSPLF EDSGTPCGKQ 800
    LQQLQQHTIQ NPHNHLSPRR FSRSARSSIS YVSSSSDRRG NSISSRSTSD 850
    SFGTPPVLGV LNVPLPPQTR EPNEPPPPCP AMSTGSNTRR SNTLTDYMHS 900
    NKASPFSSRR SSHIGRRSST PETENAFSAT PRASLDGQML GKSLKEGSTS 950
    QYTQPRMNSF PKANETIQTP TSSNNEWSRQ SVTSNTDSFD SLQSNFALEL 1000
    EPLLTPRSLY MPWPTSTVRA FAEFFYTGQV NSKWLLAPVA LDLLVMAKIY 1050
    EIPLLYKLIL EVLYSILAKK EESLSLICTS LMETFRTKTL NSYKGDEEKT 1100
    NTYLTSNDNY QELLKLKVSL ENIDNGYYDP DLLRKQSRAQ SSSTQESSGS 1150
    ANGEKTATGA GSLETSSTNV PTVFAGGPRD SHNSVGSIGF PNSMNIQGSR 1200
    RSTSGFSPRV KMKSSLSKEI DPKTFYEEYE PKEGKSFDDN DDQQTNIGSF 1250
    NLHLFDMNYG SISSSSTNSI SSSDLEEKEE QEQLQDLLEI EREDSAEILD 1300
    ARFRNKEDDK VTKDISNDKK RNYLPHEKNN LKAKEGKETR DVREEEEEFD 1350
    FGLGMLSLNK IKREAKHVDK VDDSVDPLFK SSAFPQSPIR AYGSTTRTSS 1400
    ASGKPFRDNR SFNAFSVLTL ENMASANALP PVDYVIKSIY RTTVLVNDIR 1450
    LMVRCMDCIE LSKNLRALKK KTMEDISKLK GISKPSP 1487
    Length:1,487
    Mass (Da):166,971
    Last modified:September 21, 2011 - v2
    Checksum:i31073D68ACA4AF61
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti262 – 2643QSE → HPK in CAA62947. (PubMed:9046087)Curated
    Sequence conflicti262 – 2643QSE → HPK in CAA96909. (PubMed:9169869)Curated
    Sequence conflicti403 – 4031A → R in CAA62947. (PubMed:9046087)Curated
    Sequence conflicti403 – 4031A → R in CAA96909. (PubMed:9169869)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X91837 Genomic DNA. Translation: CAA62947.1.
    Z72719 Genomic DNA. Translation: CAA96909.1.
    BK006941 Genomic DNA. Translation: DAA07917.2.
    PIRiS62048.
    RefSeqiNP_011318.2. NM_001181062.2.

    Genome annotation databases

    EnsemblFungiiYGL197W; YGL197W; YGL197W.
    GeneIDi852678.
    KEGGisce:YGL197W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X91837 Genomic DNA. Translation: CAA62947.1 .
    Z72719 Genomic DNA. Translation: CAA96909.1 .
    BK006941 Genomic DNA. Translation: DAA07917.2 .
    PIRi S62048.
    RefSeqi NP_011318.2. NM_001181062.2.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33060. 77 interactions.
    DIPi DIP-6381N.
    IntActi P53094. 24 interactions.
    MINTi MINT-598198.
    STRINGi 4932.YGL197W.

    Proteomic databases

    MaxQBi P53094.
    PaxDbi P53094.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL197W ; YGL197W ; YGL197W .
    GeneIDi 852678.
    KEGGi sce:YGL197W.

    Organism-specific databases

    CYGDi YGL197w.
    SGDi S000003165. MDS3.

    Phylogenomic databases

    eggNOGi NOG41848.
    GeneTreei ENSGT00530000066388.
    HOGENOMi HOG000113513.
    OMAi HDYGSHR.
    OrthoDBi EOG7HQNHW.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30678-MONOMER.

    Miscellaneous databases

    NextBioi 971987.

    Gene expression databases

    Genevestigatori P53094.

    Family and domain databases

    Gene3Di 2.120.10.80. 3 hits.
    InterProi IPR015915. Kelch-typ_b-propeller.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequencing of a 40.5 kb fragment located on the left arm of chromosome VII from Saccharomyces cerevisiae."
      Coglievina M., Klima R., Bertani I., Delneri D., Zaccaria P., Bruschi C.V.
      Yeast 13:55-64(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 262-264 AND 403.
      Strain: ATCC 204508 / S288c.
    4. "Identification of a new class of negative regulators affecting sporulation-specific gene expression in yeast."
      Benni M.L., Neigeborn L.
      Genetics 147:1351-1366(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    5. "Candida albicans Mds3p, a conserved regulator of pH responses and virulence identified through insertional mutagenesis."
      Davis D.A., Bruno V.M., Loza L., Filler S.G., Mitchell A.P.
      Genetics 162:1573-1581(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-639, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-621; SER-693 AND SER-781, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-621; SER-693; SER-698 AND SER-904, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "The Ras/cAMP pathway and the CDK-like kinase Ime2 regulate the MAPK Smk1 and spore morphogenesis in Saccharomyces cerevisiae."
      McDonald C.M., Wagner M., Dunham M.J., Shin M.E., Ahmed N.T., Winter E.
      Genetics 181:511-523(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-472; SER-475; SER-621; SER-693; SER-744; SER-747; SER-756; SER-757; SER-781; THR-785; SER-787; SER-900; THR-930 AND SER-1187, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMDS3_YEAST
    AccessioniPrimary (citable) accession number: P53094
    Secondary accession number(s): D6VTV6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: September 21, 2011
    Last modified: October 1, 2014
    This is version 121 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 396 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3