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P53094 (MDS3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Negative regulator of sporulation MDS3
Alternative name(s):
MCK1 dosage suppressor 3
Gene names
Name:MDS3
Ordered Locus Names:YGL197W
ORF Names:G1307
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1487 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Negatively regulates early sporulation-specific genes. Seems to exert its function by positively regulating the Ras/cAMP pathway. Required for growth under alkaline conditions. Acts synergetically with PMD1. Ref.5 Ref.11

Subcellular location

Cytoplasm Ref.6.

Miscellaneous

Present with 396 molecules/cell in log phase SD medium.

Sequence similarities

Contains 3 Kelch repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 14871487Negative regulator of sporulation MDS3
PRO_0000119137

Regions

Repeat171 – 22656Kelch 1
Repeat234 – 28754Kelch 2
Repeat371 – 41949Kelch 3

Amino acid modifications

Modified residue4721Phosphoserine Ref.12
Modified residue4751Phosphoserine Ref.12
Modified residue6211Phosphoserine Ref.9 Ref.10 Ref.12
Modified residue6391Phosphoserine Ref.8
Modified residue6931Phosphoserine Ref.9 Ref.10 Ref.12
Modified residue6981Phosphoserine Ref.10
Modified residue7441Phosphoserine Ref.12
Modified residue7471Phosphoserine Ref.12
Modified residue7561Phosphoserine Ref.12
Modified residue7571Phosphoserine Ref.12
Modified residue7811Phosphoserine Ref.9 Ref.12
Modified residue7851Phosphothreonine Ref.12
Modified residue7871Phosphoserine Ref.12
Modified residue9001Phosphoserine Ref.12
Modified residue9041Phosphoserine Ref.10
Modified residue9301Phosphothreonine Ref.12
Modified residue11871Phosphoserine Ref.12

Experimental info

Sequence conflict262 – 2643QSE → HPK in CAA62947. Ref.1
Sequence conflict262 – 2643QSE → HPK in CAA96909. Ref.2
Sequence conflict4031A → R in CAA62947. Ref.1
Sequence conflict4031A → R in CAA96909. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P53094 [UniParc].

Last modified September 21, 2011. Version 2.
Checksum: 31073D68ACA4AF61

FASTA1,487166,971
        10         20         30         40         50         60 
MPLLQPSTCF CYPLKLPPLP LTSDSNEFDE CARKRLTLDY RTGSAVTLTR SNIFVHGGLT 

        70         80         90        100        110        120 
IPLNLPVVNS MQLQKELILF FAKEKNNGSS FRNLNEWISK ETFFLDLMSR TWYRVKTSFD 

       130        140        150        160        170        180 
QRTEELLKAE SSSAKADNDT NEIRTDIKKG KSLESPLKER LFHSLCYLDG CLYIFGGLTV 

       190        200        210        220        230        240 
SPQSGYELIA TNELWKLDLN TKKWSLLSDD PQIARRFNHT MHVKNENNDN RDTKLIIVGG 

       250        260        270        280        290        300 
LNNMDQPVKK IDIYNISQNC WQSETIPKQP MEITTNVNGI PLALSKDQNF SILVENNEAN 

       310        320        330        340        350        360 
VPALAFYMRS DQIDEYLGKD SSKIKENSPI VALPLLSESQ GIRMPSNPAL PKKLLNVPYE 

       370        380        390        400        410        420 
LLAPTGDYFG FNIIIGGFHP NYQSSNFHCF IYDINSGKWS RVATACPDCD INKHRFWRVF 

       430        440        450        460        470        480 
VWKSHHQTIL LGTKTDDYYS PSVQRFDHLS TFGLPLVNIF NKTIQLPHHK ISASSLPIPI 

       490        500        510        520        530        540 
ENFAKHKDTP LKKVSFTSSA TSQFENYIRY IAPPLEMSSI QSVFPPYAMV LGKDALEIYG 

       550        560        570        580        590        600 
KPLSDFEFIT SEGDSIGIPC YLLRKRWGRY FDMLLSQSYT KVCADYETTD TQSTLIKFSP 

       610        620        630        640        650        660 
HSSRNSSKAV RQEGRLSSSG SLDNYFEKNF PIFARTSVSE AQNTQPQVAN ADAKAPNTPS 

       670        680        690        700        710        720 
TSDEPSSSSS SDLYSTPHYQ RNNDEEDDED PVSPKPVSKS NSIYRPIRKT ESSSTTSSSN 

       730        740        750        760        770        780 
GMIFRVPFKE KAAVTSNTEA LLESNLSLQE LSRRRSSLMS IPSGELLRSS ISEAEHQRRA 

       790        800        810        820        830        840 
SHPLTSSPLF EDSGTPCGKQ LQQLQQHTIQ NPHNHLSPRR FSRSARSSIS YVSSSSDRRG 

       850        860        870        880        890        900 
NSISSRSTSD SFGTPPVLGV LNVPLPPQTR EPNEPPPPCP AMSTGSNTRR SNTLTDYMHS 

       910        920        930        940        950        960 
NKASPFSSRR SSHIGRRSST PETENAFSAT PRASLDGQML GKSLKEGSTS QYTQPRMNSF 

       970        980        990       1000       1010       1020 
PKANETIQTP TSSNNEWSRQ SVTSNTDSFD SLQSNFALEL EPLLTPRSLY MPWPTSTVRA 

      1030       1040       1050       1060       1070       1080 
FAEFFYTGQV NSKWLLAPVA LDLLVMAKIY EIPLLYKLIL EVLYSILAKK EESLSLICTS 

      1090       1100       1110       1120       1130       1140 
LMETFRTKTL NSYKGDEEKT NTYLTSNDNY QELLKLKVSL ENIDNGYYDP DLLRKQSRAQ 

      1150       1160       1170       1180       1190       1200 
SSSTQESSGS ANGEKTATGA GSLETSSTNV PTVFAGGPRD SHNSVGSIGF PNSMNIQGSR 

      1210       1220       1230       1240       1250       1260 
RSTSGFSPRV KMKSSLSKEI DPKTFYEEYE PKEGKSFDDN DDQQTNIGSF NLHLFDMNYG 

      1270       1280       1290       1300       1310       1320 
SISSSSTNSI SSSDLEEKEE QEQLQDLLEI EREDSAEILD ARFRNKEDDK VTKDISNDKK 

      1330       1340       1350       1360       1370       1380 
RNYLPHEKNN LKAKEGKETR DVREEEEEFD FGLGMLSLNK IKREAKHVDK VDDSVDPLFK 

      1390       1400       1410       1420       1430       1440 
SSAFPQSPIR AYGSTTRTSS ASGKPFRDNR SFNAFSVLTL ENMASANALP PVDYVIKSIY 

      1450       1460       1470       1480 
RTTVLVNDIR LMVRCMDCIE LSKNLRALKK KTMEDISKLK GISKPSP 

« Hide

References

« Hide 'large scale' references
[1]"Sequencing of a 40.5 kb fragment located on the left arm of chromosome VII from Saccharomyces cerevisiae."
Coglievina M., Klima R., Bertani I., Delneri D., Zaccaria P., Bruschi C.V.
Yeast 13:55-64(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 262-264 AND 403.
Strain: ATCC 204508 / S288c.
[4]"Identification of a new class of negative regulators affecting sporulation-specific gene expression in yeast."
Benni M.L., Neigeborn L.
Genetics 147:1351-1366(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[5]"Candida albicans Mds3p, a conserved regulator of pH responses and virulence identified through insertional mutagenesis."
Davis D.A., Bruno V.M., Loza L., Filler S.G., Mitchell A.P.
Genetics 162:1573-1581(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-639, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: YAL6B.
[9]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-621; SER-693 AND SER-781, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[10]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-621; SER-693; SER-698 AND SER-904, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"The Ras/cAMP pathway and the CDK-like kinase Ime2 regulate the MAPK Smk1 and spore morphogenesis in Saccharomyces cerevisiae."
McDonald C.M., Wagner M., Dunham M.J., Shin M.E., Ahmed N.T., Winter E.
Genetics 181:511-523(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[12]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-472; SER-475; SER-621; SER-693; SER-744; SER-747; SER-756; SER-757; SER-781; THR-785; SER-787; SER-900; THR-930 AND SER-1187, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X91837 Genomic DNA. Translation: CAA62947.1.
Z72719 Genomic DNA. Translation: CAA96909.1.
BK006941 Genomic DNA. Translation: DAA07917.2.
PIRS62048.
RefSeqNP_011318.2. NM_001181062.2.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33060. 76 interactions.
DIPDIP-6381N.
IntActP53094. 24 interactions.
MINTMINT-598198.
STRING4932.YGL197W.

Proteomic databases

PaxDbP53094.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGL197W; YGL197W; YGL197W.
GeneID852678.
KEGGsce:YGL197W.

Organism-specific databases

CYGDYGL197w.
SGDS000003165. MDS3.

Phylogenomic databases

eggNOGNOG41848.
GeneTreeENSGT00530000066388.
HOGENOMHOG000113513.
OMAHDYGSHR.
OrthoDBEOG7HQNHW.

Enzyme and pathway databases

BioCycYEAST:G3O-30678-MONOMER.

Gene expression databases

GenevestigatorP53094.

Family and domain databases

Gene3D2.120.10.80. 3 hits.
InterProIPR015915. Kelch-typ_b-propeller.
[Graphical view]
ProtoNetSearch...

Other

NextBio971987.

Entry information

Entry nameMDS3_YEAST
AccessionPrimary (citable) accession number: P53094
Secondary accession number(s): D6VTV6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 21, 2011
Last modified: March 19, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families