Reviewed, UniProtKB/Swiss-Prot P53094 (MDS3_YEAST)

Names and origin

Protein names

Recommended name:
    Negative regulator of sporulation MDS3
Alternative name(s):
    MCK1 dosage suppressor 3

Gene names

Name:	MDS3
Ordered Locus Names:	YGL197W
ORF Names:	G1307

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1487 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Negatively regulates early sporulation-specific genes. Seems to exert its function by positively regulating the Ras/cAMP pathway. Required for growth under alkaline conditions. Acts synergetically with PMD1. Ref.4 Ref.11
Subcellular location	Cytoplasm.
Miscellaneous	Present with 396 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Contains 3 Kelch repeats.

Ontologies

Keywords
Biological process	Meiosis Sporulation
Cellular component	Cytoplasm
Domain	Kelch repeat Repeat
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	meiosis Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of sporulation resulting in formation of a cellular spore Ref.3 Inferred from genetic interaction. Source: SGD sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1487	1487	Negative regulator of sporulation MDS3		PRO_0000119137
Regions
	Repeat	171 – 226	56	Kelch 1
	Repeat	234 – 287	54	Kelch 2
	Repeat	371 – 419	49	Kelch 3
Amino acid modifications
	Modified residue	474	1	Phosphoserine Ref.7 Ref.10
	Modified residue	618	1	Phosphoserine Ref.10
	Modified residue	621	1	Phosphoserine Ref.10 Ref.8
	Modified residue	636	1	Phosphothreonine Ref.10
	Modified residue	637	1	Phosphoserine Ref.10
	Modified residue	639	1	Phosphoserine Ref.7
	Modified residue	667	1	Phosphoserine Ref.10
	Modified residue	668	1	Phosphoserine Ref.9
	Modified residue	693	1	Phosphoserine Ref.10 Ref.8 Ref.9
	Modified residue	698	1	Phosphoserine Ref.10
	Modified residue	747	1	Phosphoserine Ref.10
	Modified residue	757	1	Phosphoserine Ref.10
	Modified residue	763	1	Phosphoserine Ref.10
	Modified residue	769	1	Phosphoserine Ref.10
	Modified residue	770	1	Phosphoserine Ref.9
	Modified residue	781	1	Phosphoserine Ref.10 Ref.8
	Modified residue	828	1	Phosphoserine Ref.10
	Modified residue	854	1	Phosphothreonine Ref.10
	Modified residue	904	1	Phosphoserine Ref.10
	Modified residue	918	1	Phosphoserine Ref.10
	Modified residue	919	1	Phosphoserine Ref.10 Ref.9
	Modified residue	920	1	Phosphothreonine Ref.10 Ref.9
	Modified residue	934	1	Phosphoserine Ref.10
	Modified residue	1156	1	Phosphothreonine Ref.10
	Modified residue	1162	1	Phosphoserine Ref.10

Sequences

	Sequence		Length	Mass (Da)
	P53094-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 768AEFBAB796E447	FASTA	1,487	167,074
	10 20 30 40 50 60 MPLLQPSTCF CYPLKLPPLP LTSDSNEFDE CARKRLTLDY RTGSAVTLTR SNIFVHGGLT 70 80 90 100 110 120 IPLNLPVVNS MQLQKELILF FAKEKNNGSS FRNLNEWISK ETFFLDLMSR TWYRVKTSFD 130 140 150 160 170 180 QRTEELLKAE SSSAKADNDT NEIRTDIKKG KSLESPLKER LFHSLCYLDG CLYIFGGLTV 190 200 210 220 230 240 SPQSGYELIA TNELWKLDLN TKKWSLLSDD PQIARRFNHT MHVKNENNDN RDTKLIIVGG 250 260 270 280 290 300 LNNMDQPVKK IDIYNISQNC WHPKTIPKQP MEITTNVNGI PLALSKDQNF SILVENNEAN 310 320 330 340 350 360 VPALAFYMRS DQIDEYLGKD SSKIKENSPI VALPLLSESQ GIRMPSNPAL PKKLLNVPYE 370 380 390 400 410 420 LLAPTGDYFG FNIIIGGFHP NYQSSNFHCF IYDINSGKWS RVRTACPDCD INKHRFWRVF 430 440 450 460 470 480 VWKSHHQTIL LGTKTDDYYS PSVQRFDHLS TFGLPLVNIF NKTIQLPHHK ISASSLPIPI 490 500 510 520 530 540 ENFAKHKDTP LKKVSFTSSA TSQFENYIRY IAPPLEMSSI QSVFPPYAMV LGKDALEIYG 550 560 570 580 590 600 KPLSDFEFIT SEGDSIGIPC YLLRKRWGRY FDMLLSQSYT KVCADYETTD TQSTLIKFSP 610 620 630 640 650 660 HSSRNSSKAV RQEGRLSSSG SLDNYFEKNF PIFARTSVSE AQNTQPQVAN ADAKAPNTPS 670 680 690 700 710 720 TSDEPSSSSS SDLYSTPHYQ RNNDEEDDED PVSPKPVSKS NSIYRPIRKT ESSSTTSSSN 730 740 750 760 770 780 GMIFRVPFKE KAAVTSNTEA LLESNLSLQE LSRRRSSLMS IPSGELLRSS ISEAEHQRRA 790 800 810 820 830 840 SHPLTSSPLF EDSGTPCGKQ LQQLQQHTIQ NPHNHLSPRR FSRSARSSIS YVSSSSDRRG 850 860 870 880 890 900 NSISSRSTSD SFGTPPVLGV LNVPLPPQTR EPNEPPPPCP AMSTGSNTRR SNTLTDYMHS 910 920 930 940 950 960 NKASPFSSRR SSHIGRRSST PETENAFSAT PRASLDGQML GKSLKEGSTS QYTQPRMNSF 970 980 990 1000 1010 1020 PKANETIQTP TSSNNEWSRQ SVTSNTDSFD SLQSNFALEL EPLLTPRSLY MPWPTSTVRA 1030 1040 1050 1060 1070 1080 FAEFFYTGQV NSKWLLAPVA LDLLVMAKIY EIPLLYKLIL EVLYSILAKK EESLSLICTS 1090 1100 1110 1120 1130 1140 LMETFRTKTL NSYKGDEEKT NTYLTSNDNY QELLKLKVSL ENIDNGYYDP DLLRKQSRAQ 1150 1160 1170 1180 1190 1200 SSSTQESSGS ANGEKTATGA GSLETSSTNV PTVFAGGPRD SHNSVGSIGF PNSMNIQGSR 1210 1220 1230 1240 1250 1260 RSTSGFSPRV KMKSSLSKEI DPKTFYEEYE PKEGKSFDDN DDQQTNIGSF NLHLFDMNYG 1270 1280 1290 1300 1310 1320 SISSSSTNSI SSSDLEEKEE QEQLQDLLEI EREDSAEILD ARFRNKEDDK VTKDISNDKK 1330 1340 1350 1360 1370 1380 RNYLPHEKNN LKAKEGKETR DVREEEEEFD FGLGMLSLNK IKREAKHVDK VDDSVDPLFK 1390 1400 1410 1420 1430 1440 SSAFPQSPIR AYGSTTRTSS ASGKPFRDNR SFNAFSVLTL ENMASANALP PVDYVIKSIY 1450 1460 1470 1480 RTTVLVNDIR LMVRCMDCIE LSKNLRALKK KTMEDISKLK GISKPSP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing of a 40.5 kb fragment located on the left arm of chromosome VII from Saccharomyces cerevisiae." Coglievina M., Klima R., Bertani I., Delneri D., Zaccaria P., Bruschi C.V. Yeast 13:55-64(1997) [PubMed: 9046087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Identification of a new class of negative regulators affecting sporulation-specific gene expression in yeast." Benni M.L., Neigeborn L. Genetics 147:1351-1366(1997) [PubMed: 9383076] [Abstract] Cited for: CHARACTERIZATION.
[4]	"Candida albicans Mds3p, a conserved regulator of pH responses and virulence identified through insertional mutagenesis." Davis D.A., Bruno V.M., Loza L., Filler S.G., Mitchell A.P. Genetics 162:1573-1581(2002) [PubMed: 12524333] [Abstract] Cited for: FUNCTION.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-474 AND SER-639, MASS SPECTROMETRY.
[8]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-621; SER-693 AND SER-781, MASS SPECTROMETRY.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-668; SER-693; SER-770; SER-919 AND THR-920, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-474; SER-618; SER-621; THR-636; SER-637; SER-667; SER-693; SER-698; SER-747; SER-757; SER-763; SER-769; SER-781; SER-828; THR-854; SER-904; SER-918; SER-919; THR-920; SER-934; THR-1156 AND SER-1162, MASS SPECTROMETRY.
[11]	"The Ras/cAMP pathway and the CDK-like kinase Ime2 regulate the MAPK Smk1 and spore morphogenesis in Saccharomyces cerevisiae." McDonald C.M., Wagner M., Dunham M.J., Shin M.E., Ahmed N.T., Winter E. Genetics 181:511-523(2009) [PubMed: 19087957] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X91837 Genomic DNA. Translation: CAA62947.1. Z72719 Genomic DNA. Translation: CAA96909.1.
PIR	S62048.
RefSeq	NP_011318.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:6381N.
IntAct	P53094. 18 interactions.
STRING	P53094.
Proteomic databases
PRIDE	P53094.
Genome annotation databases
Ensembl	YGL197W; YGL197W; YGL197W; Saccharomyces cerevisiae. [Genome view]
GeneID	852678.
KEGG	sce:YGL197W.
NMPDR	fig|4932.3.peg.2421.
Organism-specific databases
CYGD	YGL197w.
SGD	S000003165. MDS3.
Phylogenomic databases
HOGENOM	P53094.
OMA	GGMVFRV
OrthoDB	EOG9Q2FXX
Gene expression databases
ArrayExpress	P53094.
Genevestigator	P53094.
GermOnline	YGL197W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011043. Gal_Oxase/kelch_b-propeller. IPR015915. Kelch-typ_b-propeller. IPR006652. Kelch_1. [Graphical view]
Gene3D	G3DSA:2.120.10.80. Kelch-typ_b-propeller. 1 hit.
Pfam	PF01344. Kelch_1. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	971987.

Entry information

Entry name

MDS3_YEAST

Accession

Primary (citable) accession number: P53094

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 24, 2009
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)