Negative regulator of sporulation MDS3 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P53094 (MDS3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 109. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Negative regulator of sporulation MDS3

Alternative name(s):
MCK1 dosage suppressor 3

Gene names

Name:	MDS3
Ordered Locus Names:	YGL197W
ORF Names:	G1307

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	1487 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Negatively regulates early sporulation-specific genes. Seems to exert its function by positively regulating the Ras/cAMP pathway. Required for growth under alkaline conditions. Acts synergetically with PMD1. Ref.5 Ref.12
Subcellular location	Cytoplasm Ref.6.
Miscellaneous	Present with 396 molecules/cell in log phase SD medium.
Sequence similarities	Contains 3 Kelch repeats.

Ontologies

Keywords
Biological process	Meiosis Sporulation
Cellular component	Cytoplasm
Domain	Kelch repeat Repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	TOR signaling cascade Inferred from mutant phenotype PubMed 20457806. Source: SGD meiosis Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of sporulation resulting in formation of a cellular spore Inferred from genetic interaction Ref.4. Source: SGD sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	mitochondrion Inferred from direct assay PubMed 14576278 PubMed 16823961. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1487

1487

Negative regulator of sporulation MDS3

PRO_0000119137

Regions

Repeat

171 – 226

Kelch 1

Repeat

234 – 287

Kelch 2

Repeat

371 – 419

Kelch 3

Amino acid modifications

Modified residue

474

Phosphoserine Ref.8 Ref.11

Modified residue

618

Phosphoserine Ref.11

Modified residue

621

Phosphoserine Ref.9 Ref.11

Modified residue

636

Phosphothreonine Ref.11

Modified residue

637

Phosphoserine Ref.11

Modified residue

639

Phosphoserine Ref.8

Modified residue

667

Phosphoserine Ref.11

Modified residue

668

Phosphoserine Ref.10

Modified residue

693

Phosphoserine Ref.9 Ref.10 Ref.11

Modified residue

698

Phosphoserine Ref.11

Modified residue

747

Phosphoserine Ref.11

Modified residue

757

Phosphoserine Ref.11

Modified residue

763

Phosphoserine Ref.11

Modified residue

769

Phosphoserine Ref.11

Modified residue

770

Phosphoserine Ref.10

Modified residue

781

Phosphoserine Ref.9 Ref.11

Modified residue

828

Phosphoserine Ref.11

Modified residue

854

Phosphothreonine Ref.11

Modified residue

904

Phosphoserine Ref.11

Modified residue

918

Phosphoserine Ref.11

Modified residue

919

Phosphoserine Ref.10 Ref.11

Modified residue

920

Phosphothreonine Ref.10 Ref.11

Modified residue

934

Phosphoserine Ref.11

Modified residue

1156

Phosphothreonine Ref.11

Modified residue

1162

Phosphoserine Ref.11

Experimental info

Sequence conflict

262 – 264

QSE → HPK in CAA62947. Ref.1

Sequence conflict

262 – 264

QSE → HPK in CAA96909. Ref.2

Sequence conflict

403

A → R in CAA62947. Ref.1

Sequence conflict

403

A → R in CAA96909. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P53094 [UniParc].

Last modified September 21, 2011. Version 2.
Checksum: 31073D68ACA4AF61
Show »

FASTA

1,487

166,971

        10         20         30         40         50         60 
MPLLQPSTCF CYPLKLPPLP LTSDSNEFDE CARKRLTLDY RTGSAVTLTR SNIFVHGGLT 

        70         80         90        100        110        120 
IPLNLPVVNS MQLQKELILF FAKEKNNGSS FRNLNEWISK ETFFLDLMSR TWYRVKTSFD 

       130        140        150        160        170        180 
QRTEELLKAE SSSAKADNDT NEIRTDIKKG KSLESPLKER LFHSLCYLDG CLYIFGGLTV 

       190        200        210        220        230        240 
SPQSGYELIA TNELWKLDLN TKKWSLLSDD PQIARRFNHT MHVKNENNDN RDTKLIIVGG 

       250        260        270        280        290        300 
LNNMDQPVKK IDIYNISQNC WQSETIPKQP MEITTNVNGI PLALSKDQNF SILVENNEAN 

       310        320        330        340        350        360 
VPALAFYMRS DQIDEYLGKD SSKIKENSPI VALPLLSESQ GIRMPSNPAL PKKLLNVPYE 

       370        380        390        400        410        420 
LLAPTGDYFG FNIIIGGFHP NYQSSNFHCF IYDINSGKWS RVATACPDCD INKHRFWRVF 

       430        440        450        460        470        480 
VWKSHHQTIL LGTKTDDYYS PSVQRFDHLS TFGLPLVNIF NKTIQLPHHK ISASSLPIPI 

       490        500        510        520        530        540 
ENFAKHKDTP LKKVSFTSSA TSQFENYIRY IAPPLEMSSI QSVFPPYAMV LGKDALEIYG 

       550        560        570        580        590        600 
KPLSDFEFIT SEGDSIGIPC YLLRKRWGRY FDMLLSQSYT KVCADYETTD TQSTLIKFSP 

       610        620        630        640        650        660 
HSSRNSSKAV RQEGRLSSSG SLDNYFEKNF PIFARTSVSE AQNTQPQVAN ADAKAPNTPS 

       670        680        690        700        710        720 
TSDEPSSSSS SDLYSTPHYQ RNNDEEDDED PVSPKPVSKS NSIYRPIRKT ESSSTTSSSN 

       730        740        750        760        770        780 
GMIFRVPFKE KAAVTSNTEA LLESNLSLQE LSRRRSSLMS IPSGELLRSS ISEAEHQRRA 

       790        800        810        820        830        840 
SHPLTSSPLF EDSGTPCGKQ LQQLQQHTIQ NPHNHLSPRR FSRSARSSIS YVSSSSDRRG 

       850        860        870        880        890        900 
NSISSRSTSD SFGTPPVLGV LNVPLPPQTR EPNEPPPPCP AMSTGSNTRR SNTLTDYMHS 

       910        920        930        940        950        960 
NKASPFSSRR SSHIGRRSST PETENAFSAT PRASLDGQML GKSLKEGSTS QYTQPRMNSF 

       970        980        990       1000       1010       1020 
PKANETIQTP TSSNNEWSRQ SVTSNTDSFD SLQSNFALEL EPLLTPRSLY MPWPTSTVRA 

      1030       1040       1050       1060       1070       1080 
FAEFFYTGQV NSKWLLAPVA LDLLVMAKIY EIPLLYKLIL EVLYSILAKK EESLSLICTS 

      1090       1100       1110       1120       1130       1140 
LMETFRTKTL NSYKGDEEKT NTYLTSNDNY QELLKLKVSL ENIDNGYYDP DLLRKQSRAQ 

      1150       1160       1170       1180       1190       1200 
SSSTQESSGS ANGEKTATGA GSLETSSTNV PTVFAGGPRD SHNSVGSIGF PNSMNIQGSR 

      1210       1220       1230       1240       1250       1260 
RSTSGFSPRV KMKSSLSKEI DPKTFYEEYE PKEGKSFDDN DDQQTNIGSF NLHLFDMNYG 

      1270       1280       1290       1300       1310       1320 
SISSSSTNSI SSSDLEEKEE QEQLQDLLEI EREDSAEILD ARFRNKEDDK VTKDISNDKK 

      1330       1340       1350       1360       1370       1380 
RNYLPHEKNN LKAKEGKETR DVREEEEEFD FGLGMLSLNK IKREAKHVDK VDDSVDPLFK 

      1390       1400       1410       1420       1430       1440 
SSAFPQSPIR AYGSTTRTSS ASGKPFRDNR SFNAFSVLTL ENMASANALP PVDYVIKSIY 

      1450       1460       1470       1480 
RTTVLVNDIR LMVRCMDCIE LSKNLRALKK KTMEDISKLK GISKPSP

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing of a 40.5 kb fragment located on the left arm of chromosome VII from Saccharomyces cerevisiae." Coglievina M., Klima R., Bertani I., Delneri D., Zaccaria P., Bruschi C.V. Yeast 13:55-64(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 262-264 AND 403. Strain: ATCC 204508 / S288c.
[4]	"Identification of a new class of negative regulators affecting sporulation-specific gene expression in yeast." Benni M.L., Neigeborn L. Genetics 147:1351-1366(1997) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Candida albicans Mds3p, a conserved regulator of pH responses and virulence identified through insertional mutagenesis." Davis D.A., Bruno V.M., Loza L., Filler S.G., Mitchell A.P. Genetics 162:1573-1581(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-474 AND SER-639, MASS SPECTROMETRY. Strain: YAL6B.
[9]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-621; SER-693 AND SER-781, MASS SPECTROMETRY. Strain: ADR376.
[10]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-668; SER-693; SER-770; SER-919 AND THR-920, MASS SPECTROMETRY.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-474; SER-618; SER-621; THR-636; SER-637; SER-667; SER-693; SER-698; SER-747; SER-757; SER-763; SER-769; SER-781; SER-828; THR-854; SER-904; SER-918; SER-919; THR-920; SER-934; THR-1156 AND SER-1162, MASS SPECTROMETRY.
[12]	"The Ras/cAMP pathway and the CDK-like kinase Ime2 regulate the MAPK Smk1 and spore morphogenesis in Saccharomyces cerevisiae." McDonald C.M., Wagner M., Dunham M.J., Shin M.E., Ahmed N.T., Winter E. Genetics 181:511-523(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X91837 Genomic DNA. Translation: CAA62947.1. Z72719 Genomic DNA. Translation: CAA96909.1. BK006941 Genomic DNA. Translation: DAA07917.2.
PIR	S62048.
RefSeq	NP_011318.2. NM_001181062.2.
3D structure databases
ProteinModelPortal	P53094.
SMR	P53094. Positions 169-266.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6381N.
IntAct	P53094. 20 interactions.
MINT	MINT-598198.
STRING	4932.YGL197W.
Proteomic databases
PaxDb	P53094.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL197W; YGL197W; YGL197W.
GeneID	852678.
KEGG	sce:YGL197W.
Organism-specific databases
CYGD	YGL197w.
SGD	S000003165. MDS3.
Phylogenomic databases
eggNOG	NOG41848.
GeneTree	ENSGT00530000066388.
HOGENOM	HOG000113513.
OMA	GGMVFRV.
OrthoDB	EOG437VP0.
Gene expression databases
Genevestigator	P53094.
GermOnline	YGL197W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	2.120.10.80. 3 hits.
InterPro	IPR015915. Kelch-typ_b-propeller. [Graphical view]
ProtoNet	Search...
Other
NextBio	971987.

Entry information

Entry name

MDS3_YEAST

Accession

Primary (citable) accession number: P53094
Secondary accession number(s): D6VTV6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	September 21, 2011
Last modified:	May 1, 2013
This is version 109 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents