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P53094

- MDS3_YEAST

UniProt

P53094 - MDS3_YEAST

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Protein

Negative regulator of sporulation MDS3

Gene

MDS3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Negatively regulates early sporulation-specific genes. Seems to exert its function by positively regulating the Ras/cAMP pathway. Required for growth under alkaline conditions. Acts synergetically with PMD1.2 Publications

GO - Biological processi

  1. meiotic nuclear division Source: UniProtKB-KW
  2. negative regulation of sporulation resulting in formation of a cellular spore Source: SGD
  3. pseudohyphal growth Source: SGD
  4. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
  5. TOR signaling Source: SGD
Complete GO annotation...

Keywords - Biological processi

Meiosis, Sporulation

Enzyme and pathway databases

BioCyciYEAST:G3O-30678-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Negative regulator of sporulation MDS3
Alternative name(s):
MCK1 dosage suppressor 3
Gene namesi
Name:MDS3
Ordered Locus Names:YGL197W
ORF Names:G1307
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGL197w.
SGDiS000003165. MDS3.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14871487Negative regulator of sporulation MDS3PRO_0000119137Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei472 – 4721Phosphoserine1 Publication
Modified residuei475 – 4751Phosphoserine1 Publication
Modified residuei621 – 6211Phosphoserine3 Publications
Modified residuei639 – 6391Phosphoserine1 Publication
Modified residuei693 – 6931Phosphoserine3 Publications
Modified residuei698 – 6981Phosphoserine1 Publication
Modified residuei744 – 7441Phosphoserine1 Publication
Modified residuei747 – 7471Phosphoserine1 Publication
Modified residuei756 – 7561Phosphoserine1 Publication
Modified residuei757 – 7571Phosphoserine1 Publication
Modified residuei781 – 7811Phosphoserine2 Publications
Modified residuei785 – 7851Phosphothreonine1 Publication
Modified residuei787 – 7871Phosphoserine1 Publication
Modified residuei900 – 9001Phosphoserine1 Publication
Modified residuei904 – 9041Phosphoserine1 Publication
Modified residuei930 – 9301Phosphothreonine1 Publication
Modified residuei1187 – 11871Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53094.
PaxDbiP53094.

Expressioni

Gene expression databases

GenevestigatoriP53094.

Interactioni

Protein-protein interaction databases

BioGridi33060. 77 interactions.
DIPiDIP-6381N.
IntActiP53094. 24 interactions.
MINTiMINT-598198.
STRINGi4932.YGL197W.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati171 – 22656Kelch 1Add
BLAST
Repeati234 – 28754Kelch 2Add
BLAST
Repeati371 – 41949Kelch 3Add
BLAST

Sequence similaritiesi

Contains 3 Kelch repeats.Curated

Keywords - Domaini

Kelch repeat, Repeat

Phylogenomic databases

eggNOGiNOG41848.
GeneTreeiENSGT00530000066388.
HOGENOMiHOG000113513.
InParanoidiP53094.
OMAiHDYGSHR.
OrthoDBiEOG7HQNHW.

Family and domain databases

Gene3Di2.120.10.80. 3 hits.
InterProiIPR015915. Kelch-typ_b-propeller.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53094-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPLLQPSTCF CYPLKLPPLP LTSDSNEFDE CARKRLTLDY RTGSAVTLTR
60 70 80 90 100
SNIFVHGGLT IPLNLPVVNS MQLQKELILF FAKEKNNGSS FRNLNEWISK
110 120 130 140 150
ETFFLDLMSR TWYRVKTSFD QRTEELLKAE SSSAKADNDT NEIRTDIKKG
160 170 180 190 200
KSLESPLKER LFHSLCYLDG CLYIFGGLTV SPQSGYELIA TNELWKLDLN
210 220 230 240 250
TKKWSLLSDD PQIARRFNHT MHVKNENNDN RDTKLIIVGG LNNMDQPVKK
260 270 280 290 300
IDIYNISQNC WQSETIPKQP MEITTNVNGI PLALSKDQNF SILVENNEAN
310 320 330 340 350
VPALAFYMRS DQIDEYLGKD SSKIKENSPI VALPLLSESQ GIRMPSNPAL
360 370 380 390 400
PKKLLNVPYE LLAPTGDYFG FNIIIGGFHP NYQSSNFHCF IYDINSGKWS
410 420 430 440 450
RVATACPDCD INKHRFWRVF VWKSHHQTIL LGTKTDDYYS PSVQRFDHLS
460 470 480 490 500
TFGLPLVNIF NKTIQLPHHK ISASSLPIPI ENFAKHKDTP LKKVSFTSSA
510 520 530 540 550
TSQFENYIRY IAPPLEMSSI QSVFPPYAMV LGKDALEIYG KPLSDFEFIT
560 570 580 590 600
SEGDSIGIPC YLLRKRWGRY FDMLLSQSYT KVCADYETTD TQSTLIKFSP
610 620 630 640 650
HSSRNSSKAV RQEGRLSSSG SLDNYFEKNF PIFARTSVSE AQNTQPQVAN
660 670 680 690 700
ADAKAPNTPS TSDEPSSSSS SDLYSTPHYQ RNNDEEDDED PVSPKPVSKS
710 720 730 740 750
NSIYRPIRKT ESSSTTSSSN GMIFRVPFKE KAAVTSNTEA LLESNLSLQE
760 770 780 790 800
LSRRRSSLMS IPSGELLRSS ISEAEHQRRA SHPLTSSPLF EDSGTPCGKQ
810 820 830 840 850
LQQLQQHTIQ NPHNHLSPRR FSRSARSSIS YVSSSSDRRG NSISSRSTSD
860 870 880 890 900
SFGTPPVLGV LNVPLPPQTR EPNEPPPPCP AMSTGSNTRR SNTLTDYMHS
910 920 930 940 950
NKASPFSSRR SSHIGRRSST PETENAFSAT PRASLDGQML GKSLKEGSTS
960 970 980 990 1000
QYTQPRMNSF PKANETIQTP TSSNNEWSRQ SVTSNTDSFD SLQSNFALEL
1010 1020 1030 1040 1050
EPLLTPRSLY MPWPTSTVRA FAEFFYTGQV NSKWLLAPVA LDLLVMAKIY
1060 1070 1080 1090 1100
EIPLLYKLIL EVLYSILAKK EESLSLICTS LMETFRTKTL NSYKGDEEKT
1110 1120 1130 1140 1150
NTYLTSNDNY QELLKLKVSL ENIDNGYYDP DLLRKQSRAQ SSSTQESSGS
1160 1170 1180 1190 1200
ANGEKTATGA GSLETSSTNV PTVFAGGPRD SHNSVGSIGF PNSMNIQGSR
1210 1220 1230 1240 1250
RSTSGFSPRV KMKSSLSKEI DPKTFYEEYE PKEGKSFDDN DDQQTNIGSF
1260 1270 1280 1290 1300
NLHLFDMNYG SISSSSTNSI SSSDLEEKEE QEQLQDLLEI EREDSAEILD
1310 1320 1330 1340 1350
ARFRNKEDDK VTKDISNDKK RNYLPHEKNN LKAKEGKETR DVREEEEEFD
1360 1370 1380 1390 1400
FGLGMLSLNK IKREAKHVDK VDDSVDPLFK SSAFPQSPIR AYGSTTRTSS
1410 1420 1430 1440 1450
ASGKPFRDNR SFNAFSVLTL ENMASANALP PVDYVIKSIY RTTVLVNDIR
1460 1470 1480
LMVRCMDCIE LSKNLRALKK KTMEDISKLK GISKPSP
Length:1,487
Mass (Da):166,971
Last modified:September 21, 2011 - v2
Checksum:i31073D68ACA4AF61
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti262 – 2643QSE → HPK in CAA62947. (PubMed:9046087)Curated
Sequence conflicti262 – 2643QSE → HPK in CAA96909. (PubMed:9169869)Curated
Sequence conflicti403 – 4031A → R in CAA62947. (PubMed:9046087)Curated
Sequence conflicti403 – 4031A → R in CAA96909. (PubMed:9169869)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91837 Genomic DNA. Translation: CAA62947.1.
Z72719 Genomic DNA. Translation: CAA96909.1.
BK006941 Genomic DNA. Translation: DAA07917.2.
PIRiS62048.
RefSeqiNP_011318.2. NM_001181062.2.

Genome annotation databases

EnsemblFungiiYGL197W; YGL197W; YGL197W.
GeneIDi852678.
KEGGisce:YGL197W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91837 Genomic DNA. Translation: CAA62947.1 .
Z72719 Genomic DNA. Translation: CAA96909.1 .
BK006941 Genomic DNA. Translation: DAA07917.2 .
PIRi S62048.
RefSeqi NP_011318.2. NM_001181062.2.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33060. 77 interactions.
DIPi DIP-6381N.
IntActi P53094. 24 interactions.
MINTi MINT-598198.
STRINGi 4932.YGL197W.

Proteomic databases

MaxQBi P53094.
PaxDbi P53094.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGL197W ; YGL197W ; YGL197W .
GeneIDi 852678.
KEGGi sce:YGL197W.

Organism-specific databases

CYGDi YGL197w.
SGDi S000003165. MDS3.

Phylogenomic databases

eggNOGi NOG41848.
GeneTreei ENSGT00530000066388.
HOGENOMi HOG000113513.
InParanoidi P53094.
OMAi HDYGSHR.
OrthoDBi EOG7HQNHW.

Enzyme and pathway databases

BioCyci YEAST:G3O-30678-MONOMER.

Miscellaneous databases

NextBioi 971987.

Gene expression databases

Genevestigatori P53094.

Family and domain databases

Gene3Di 2.120.10.80. 3 hits.
InterProi IPR015915. Kelch-typ_b-propeller.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing of a 40.5 kb fragment located on the left arm of chromosome VII from Saccharomyces cerevisiae."
    Coglievina M., Klima R., Bertani I., Delneri D., Zaccaria P., Bruschi C.V.
    Yeast 13:55-64(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 262-264 AND 403.
    Strain: ATCC 204508 / S288c.
  4. "Identification of a new class of negative regulators affecting sporulation-specific gene expression in yeast."
    Benni M.L., Neigeborn L.
    Genetics 147:1351-1366(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "Candida albicans Mds3p, a conserved regulator of pH responses and virulence identified through insertional mutagenesis."
    Davis D.A., Bruno V.M., Loza L., Filler S.G., Mitchell A.P.
    Genetics 162:1573-1581(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-639, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-621; SER-693 AND SER-781, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-621; SER-693; SER-698 AND SER-904, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "The Ras/cAMP pathway and the CDK-like kinase Ime2 regulate the MAPK Smk1 and spore morphogenesis in Saccharomyces cerevisiae."
    McDonald C.M., Wagner M., Dunham M.J., Shin M.E., Ahmed N.T., Winter E.
    Genetics 181:511-523(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-472; SER-475; SER-621; SER-693; SER-744; SER-747; SER-756; SER-757; SER-781; THR-785; SER-787; SER-900; THR-930 AND SER-1187, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMDS3_YEAST
AccessioniPrimary (citable) accession number: P53094
Secondary accession number(s): D6VTV6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 21, 2011
Last modified: October 29, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 396 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3