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Protein

Negative regulator of sporulation MDS3

Gene

MDS3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Negatively regulates early sporulation-specific genes. Seems to exert its function by positively regulating the Ras/cAMP pathway. Required for growth under alkaline conditions. Acts synergetically with PMD1.2 Publications

GO - Biological processi

  • meiotic cell cycle Source: UniProtKB-KW
  • negative regulation of sporulation resulting in formation of a cellular spore Source: SGD
  • pseudohyphal growth Source: SGD
  • sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
  • TOR signaling Source: SGD
Complete GO annotation...

Keywords - Biological processi

Meiosis, Sporulation

Enzyme and pathway databases

BioCyciYEAST:G3O-30678-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Negative regulator of sporulation MDS3
Alternative name(s):
MCK1 dosage suppressor 3
Gene namesi
Name:MDS3
Ordered Locus Names:YGL197W
ORF Names:G1307
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL197W.
SGDiS000003165. MDS3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001191371 – 1487Negative regulator of sporulation MDS3Add BLAST1487

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei472PhosphoserineCombined sources1
Modified residuei475PhosphoserineCombined sources1
Modified residuei621PhosphoserineCombined sources1
Modified residuei639PhosphoserineCombined sources1
Modified residuei693PhosphoserineCombined sources1
Modified residuei698PhosphoserineCombined sources1
Modified residuei744PhosphoserineCombined sources1
Modified residuei747PhosphoserineCombined sources1
Modified residuei756PhosphoserineCombined sources1
Modified residuei757PhosphoserineCombined sources1
Modified residuei781PhosphoserineCombined sources1
Modified residuei785PhosphothreonineCombined sources1
Modified residuei787PhosphoserineCombined sources1
Modified residuei900PhosphoserineCombined sources1
Modified residuei904PhosphoserineCombined sources1
Modified residuei930PhosphothreonineCombined sources1
Modified residuei1187PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53094.
PRIDEiP53094.

PTM databases

iPTMnetiP53094.

Interactioni

Protein-protein interaction databases

BioGridi33060. 80 interactors.
DIPiDIP-6381N.
IntActiP53094. 25 interactors.
MINTiMINT-598198.

Structurei

3D structure databases

ProteinModelPortaliP53094.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati171 – 226Kelch 1Add BLAST56
Repeati234 – 287Kelch 2Add BLAST54
Repeati371 – 419Kelch 3Add BLAST49

Sequence similaritiesi

Contains 3 Kelch repeats.Curated

Keywords - Domaini

Kelch repeat, Repeat

Phylogenomic databases

GeneTreeiENSGT00530000066388.
HOGENOMiHOG000113513.
InParanoidiP53094.
OMAiHDYGSHR.
OrthoDBiEOG092C0S3Y.

Family and domain databases

Gene3Di2.120.10.80. 3 hits.
InterProiIPR015915. Kelch-typ_b-propeller.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53094-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLLQPSTCF CYPLKLPPLP LTSDSNEFDE CARKRLTLDY RTGSAVTLTR
60 70 80 90 100
SNIFVHGGLT IPLNLPVVNS MQLQKELILF FAKEKNNGSS FRNLNEWISK
110 120 130 140 150
ETFFLDLMSR TWYRVKTSFD QRTEELLKAE SSSAKADNDT NEIRTDIKKG
160 170 180 190 200
KSLESPLKER LFHSLCYLDG CLYIFGGLTV SPQSGYELIA TNELWKLDLN
210 220 230 240 250
TKKWSLLSDD PQIARRFNHT MHVKNENNDN RDTKLIIVGG LNNMDQPVKK
260 270 280 290 300
IDIYNISQNC WQSETIPKQP MEITTNVNGI PLALSKDQNF SILVENNEAN
310 320 330 340 350
VPALAFYMRS DQIDEYLGKD SSKIKENSPI VALPLLSESQ GIRMPSNPAL
360 370 380 390 400
PKKLLNVPYE LLAPTGDYFG FNIIIGGFHP NYQSSNFHCF IYDINSGKWS
410 420 430 440 450
RVATACPDCD INKHRFWRVF VWKSHHQTIL LGTKTDDYYS PSVQRFDHLS
460 470 480 490 500
TFGLPLVNIF NKTIQLPHHK ISASSLPIPI ENFAKHKDTP LKKVSFTSSA
510 520 530 540 550
TSQFENYIRY IAPPLEMSSI QSVFPPYAMV LGKDALEIYG KPLSDFEFIT
560 570 580 590 600
SEGDSIGIPC YLLRKRWGRY FDMLLSQSYT KVCADYETTD TQSTLIKFSP
610 620 630 640 650
HSSRNSSKAV RQEGRLSSSG SLDNYFEKNF PIFARTSVSE AQNTQPQVAN
660 670 680 690 700
ADAKAPNTPS TSDEPSSSSS SDLYSTPHYQ RNNDEEDDED PVSPKPVSKS
710 720 730 740 750
NSIYRPIRKT ESSSTTSSSN GMIFRVPFKE KAAVTSNTEA LLESNLSLQE
760 770 780 790 800
LSRRRSSLMS IPSGELLRSS ISEAEHQRRA SHPLTSSPLF EDSGTPCGKQ
810 820 830 840 850
LQQLQQHTIQ NPHNHLSPRR FSRSARSSIS YVSSSSDRRG NSISSRSTSD
860 870 880 890 900
SFGTPPVLGV LNVPLPPQTR EPNEPPPPCP AMSTGSNTRR SNTLTDYMHS
910 920 930 940 950
NKASPFSSRR SSHIGRRSST PETENAFSAT PRASLDGQML GKSLKEGSTS
960 970 980 990 1000
QYTQPRMNSF PKANETIQTP TSSNNEWSRQ SVTSNTDSFD SLQSNFALEL
1010 1020 1030 1040 1050
EPLLTPRSLY MPWPTSTVRA FAEFFYTGQV NSKWLLAPVA LDLLVMAKIY
1060 1070 1080 1090 1100
EIPLLYKLIL EVLYSILAKK EESLSLICTS LMETFRTKTL NSYKGDEEKT
1110 1120 1130 1140 1150
NTYLTSNDNY QELLKLKVSL ENIDNGYYDP DLLRKQSRAQ SSSTQESSGS
1160 1170 1180 1190 1200
ANGEKTATGA GSLETSSTNV PTVFAGGPRD SHNSVGSIGF PNSMNIQGSR
1210 1220 1230 1240 1250
RSTSGFSPRV KMKSSLSKEI DPKTFYEEYE PKEGKSFDDN DDQQTNIGSF
1260 1270 1280 1290 1300
NLHLFDMNYG SISSSSTNSI SSSDLEEKEE QEQLQDLLEI EREDSAEILD
1310 1320 1330 1340 1350
ARFRNKEDDK VTKDISNDKK RNYLPHEKNN LKAKEGKETR DVREEEEEFD
1360 1370 1380 1390 1400
FGLGMLSLNK IKREAKHVDK VDDSVDPLFK SSAFPQSPIR AYGSTTRTSS
1410 1420 1430 1440 1450
ASGKPFRDNR SFNAFSVLTL ENMASANALP PVDYVIKSIY RTTVLVNDIR
1460 1470 1480
LMVRCMDCIE LSKNLRALKK KTMEDISKLK GISKPSP
Length:1,487
Mass (Da):166,971
Last modified:September 21, 2011 - v2
Checksum:i31073D68ACA4AF61
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti262 – 264QSE → HPK in CAA62947 (PubMed:9046087).Curated3
Sequence conflicti262 – 264QSE → HPK in CAA96909 (PubMed:9169869).Curated3
Sequence conflicti403A → R in CAA62947 (PubMed:9046087).Curated1
Sequence conflicti403A → R in CAA96909 (PubMed:9169869).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91837 Genomic DNA. Translation: CAA62947.1.
Z72719 Genomic DNA. Translation: CAA96909.1.
BK006941 Genomic DNA. Translation: DAA07917.2.
PIRiS62048.
RefSeqiNP_011318.2. NM_001181062.2.

Genome annotation databases

EnsemblFungiiYGL197W; YGL197W; YGL197W.
GeneIDi852678.
KEGGisce:YGL197W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91837 Genomic DNA. Translation: CAA62947.1.
Z72719 Genomic DNA. Translation: CAA96909.1.
BK006941 Genomic DNA. Translation: DAA07917.2.
PIRiS62048.
RefSeqiNP_011318.2. NM_001181062.2.

3D structure databases

ProteinModelPortaliP53094.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33060. 80 interactors.
DIPiDIP-6381N.
IntActiP53094. 25 interactors.
MINTiMINT-598198.

PTM databases

iPTMnetiP53094.

Proteomic databases

MaxQBiP53094.
PRIDEiP53094.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL197W; YGL197W; YGL197W.
GeneIDi852678.
KEGGisce:YGL197W.

Organism-specific databases

EuPathDBiFungiDB:YGL197W.
SGDiS000003165. MDS3.

Phylogenomic databases

GeneTreeiENSGT00530000066388.
HOGENOMiHOG000113513.
InParanoidiP53094.
OMAiHDYGSHR.
OrthoDBiEOG092C0S3Y.

Enzyme and pathway databases

BioCyciYEAST:G3O-30678-MONOMER.

Miscellaneous databases

PROiP53094.

Family and domain databases

Gene3Di2.120.10.80. 3 hits.
InterProiIPR015915. Kelch-typ_b-propeller.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMDS3_YEAST
AccessioniPrimary (citable) accession number: P53094
Secondary accession number(s): D6VTV6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 21, 2011
Last modified: November 2, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 396 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.