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Protein

Aromatic/aminoadipate aminotransferase 1

Gene

ARO8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

General aromatic amino acid transaminase involved in several otherwise unrelated metabolic pathways. Responsible for phenylalanine and tyrosine biosynthesis. Active with glutamate, phenylalanine, tyrosine and tryptophan as amino donors and with phenylpyruvate, hydroxyphenylpyruvate, 2-oxoglutarate and pyruvate as amino acceptors. Also active with methionine, alpha-aminoadipate and leucine as amino donors when phenylpyruvate is the amino acceptor and in the reverse reactions with the corresponding oxo acids and phenylalanine as amino donor. Catalyzes the formation of methionine from 2-keto-4-methylthiobutyrate (KMTB) in the methionine salvage pathway primarily using aromatic amino acids (tyrosine, phenylalanine and tryptophan) as the amino donors. Catalyzes the formation of alpha-aminoadipate from alpha-ketoadipate in the lysine biosyntheic pathway.5 Publications

Miscellaneous

Present with 1770 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

An aromatic amino acid + 2-oxoglutarate = an aromatic oxo acid + L-glutamate.
An aromatic amino acid + 4-(methylthio)-2-oxobutanoate = an aromatic oxo acid + L-methionine.
L-2-aminoadipate + 2-oxoglutarate = 2-oxoadipate + L-glutamate.

Cofactori

Pathwayi: L-phenylalanine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-phenylalanine from phenylpyruvate (ArAT route).
Proteins known to be involved in this subpathway in this organism are:
  1. Aromatic/aminoadipate aminotransferase 1 (ARO8)
This subpathway is part of the pathway L-phenylalanine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-phenylalanine from phenylpyruvate (ArAT route), the pathway L-phenylalanine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-tyrosine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-tyrosine from (4-hydroxyphenyl)pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. Aromatic/aminoadipate aminotransferase 1 (ARO8)
This subpathway is part of the pathway L-tyrosine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tyrosine from (4-hydroxyphenyl)pyruvate, the pathway L-tyrosine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 6 of the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Methylthioribose-1-phosphate isomerase (MRI1)
  2. Methylthioribulose-1-phosphate dehydratase (MDE1)
  3. Enolase-phosphatase E1 (UTR4)
  4. Enolase-phosphatase E1 (UTR4)
  5. 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (ADI1)
  6. Branched-chain-amino-acid aminotransferase, cytosolic (BAT2), Aromatic amino acid aminotransferase 2 (ARO9), Branched-chain-amino-acid aminotransferase, mitochondrial (BAT1), Aromatic/aminoadipate aminotransferase 1 (ARO8)
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate, the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Pathwayi: L-lysine biosynthesis via AAA pathway

This protein is involved in step 5 of the subpathway that synthesizes L-alpha-aminoadipate from 2-oxoglutarate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Homocitrate synthase, cytosolic isozyme (LYS20), Homocitrate synthase, mitochondrial (LYS21)
  2. Homocitrate dehydratase, mitochondrial (ACO2)
  3. Homoaconitase, mitochondrial (LYS4)
  4. Homoisocitrate dehydrogenase, mitochondrial (LYS12)
  5. Aromatic/aminoadipate aminotransferase 1 (ARO8)
This subpathway is part of the pathway L-lysine biosynthesis via AAA pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-alpha-aminoadipate from 2-oxoglutarate, the pathway L-lysine biosynthesis via AAA pathway and in Amino-acid biosynthesis.

GO - Molecular functioni

  • 2-aminoadipate transaminase activity Source: SGD
  • aromatic-amino-acid:2-oxoglutarate aminotransferase activity Source: SGD
  • L-phenylalanine:2-oxoglutarate aminotransferase activity Source: UniProtKB-EC
  • pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  • aromatic amino acid family metabolic process Source: SGD
  • L-methionine salvage from methylthioadenosine Source: UniProtKB-UniPathway
  • L-phenylalanine biosynthetic process Source: SGD
  • lysine biosynthetic process via aminoadipic acid Source: UniProtKB-UniPathway
  • tyrosine biosynthetic process Source: SGD

Keywordsi

Molecular functionAminotransferase, Transferase
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:YGL202W-MONOMER
YEAST:YGL202W-MONOMER
BRENDAi2.6.1.57 984
ReactomeiR-SCE-71064 Lysine catabolism
R-SCE-71240 Tryptophan catabolism
UniPathwayiUPA00033; UER00031
UPA00121; UER00347
UPA00122; UER00350
UPA00904; UER00879

Names & Taxonomyi

Protein namesi
Recommended name:
Aromatic/aminoadipate aminotransferase 1
Alternative name(s):
2-aminoadipate aminotransferase (EC:2.6.1.39)
2-aminoadipate transaminase
Alpha-aminoadipate aminotransferase
Short name:
AadAT
Aromatic amino acid aminotransferase 1 (EC:2.6.1.57)
Aromatic amino acid aminotransferase I
Aromatic amino acid-requiring protein 8
Gene namesi
Name:ARO8
Ordered Locus Names:YGL202W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL202W
SGDiS000003170 ARO8

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000646781 – 500Aromatic/aminoadipate aminotransferase 1Add BLAST500

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei100PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53090
PaxDbiP53090
PRIDEiP53090

PTM databases

iPTMnetiP53090

Interactioni

Protein-protein interaction databases

BioGridi33055, 140 interactors
DIPiDIP-983N
IntActiP53090, 6 interactors
MINTiP53090
STRINGi4932.YGL202W

Structurei

Secondary structure

1500
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 12Combined sources3
Helixi15 – 19Combined sources5
Helixi24 – 29Combined sources6
Turni30 – 33Combined sources4
Beta strandi37 – 39Combined sources3
Helixi47 – 49Combined sources3
Beta strandi53 – 59Combined sources7
Beta strandi74 – 76Combined sources3
Beta strandi78 – 82Combined sources5
Helixi89 – 91Combined sources3
Helixi99 – 102Combined sources4
Helixi112 – 125Combined sources14
Beta strandi133 – 139Combined sources7
Helixi141 – 152Combined sources12
Beta strandi158 – 164Combined sources7
Helixi167 – 175Combined sources9
Beta strandi179 – 183Combined sources5
Helixi192 – 200Combined sources9
Beta strandi211 – 214Combined sources4
Turni220 – 222Combined sources3
Helixi228 – 241Combined sources14
Beta strandi244 – 248Combined sources5
Helixi252 – 254Combined sources3
Helixi263 – 271Combined sources9
Helixi277 – 283Combined sources7
Helixi288 – 291Combined sources4
Beta strandi293 – 295Combined sources3
Beta strandi297 – 303Combined sources7
Turni304 – 307Combined sources4
Helixi309 – 311Combined sources3
Beta strandi314 – 319Combined sources6
Helixi320 – 331Combined sources12
Turni332 – 334Combined sources3
Helixi339 – 382Combined sources44
Beta strandi395 – 405Combined sources11
Helixi406 – 408Combined sources3
Turni410 – 418Combined sources9
Helixi420 – 433Combined sources14
Helixi441 – 444Combined sources4
Helixi456 – 459Combined sources4
Beta strandi466 – 476Combined sources11
Helixi478 – 495Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4JE5X-ray1.91A/B/C/D1-500[»]
ProteinModelPortaliP53090
SMRiP53090
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000004594
HOGENOMiHOG000223057
InParanoidiP53090
KOiK00838
OMAiPVRCLQY
OrthoDBiEOG092C1J5R

Family and domain databases

Gene3Di3.40.640.10, 1 hit
InterProiView protein in InterPro
IPR004839 Aminotransferase_I/II
IPR015424 PyrdxlP-dep_Trfase
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF00155 Aminotran_1_2, 1 hit
SUPFAMiSSF53383 SSF53383, 2 hits

Sequencei

Sequence statusi: Complete.

P53090-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLPESKDFS YLFSDETNAR KPSPLKTCIH LFQDPNIIFL GGGLPLKDYF
60 70 80 90 100
PWDNLSVDSP KPPFPQGIGA PIDEQNCIKY TVNKDYADKS ANPSNDIPLS
110 120 130 140 150
RALQYGFSAG QPELLNFIRD HTKIIHDLKY KDWDVLATAG NTNAWESTLR
160 170 180 190 200
VFCNRGDVIL VEAHSFSSSL ASAEAQGVIT FPVPIDADGI IPEKLAKVME
210 220 230 240 250
NWTPGAPKPK LLYTIPTGQN PTGTSIADHR KEAIYKIAQK YDFLIVEDEP
260 270 280 290 300
YYFLQMNPYI KDLKEREKAQ SSPKQDHDEF LKSLANTFLS LDTEGRVIRM
310 320 330 340 350
DSFSKVLAPG TRLGWITGSS KILKPYLSLH EMTIQAPAGF TQVLVNATLS
360 370 380 390 400
RWGQKGYLDW LLGLRHEYTL KRDCAIDALY KYLPQSDAFV INPPIAGMFF
410 420 430 440 450
TVNIDASVHP EFKTKYNSDP YQLEQSLYHK VVERGVLVVP GSWFKSEGET
460 470 480 490 500
EPPQPAESKE VSNPNIIFFR GTYAAVSPEK LTEGLKRLGD TLYEEFGISK
Length:500
Mass (Da):56,178
Last modified:October 1, 1996 - v1
Checksum:iD0D111640D2C560D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13624 Genomic DNA Translation: CAA73946.1
Z72724 Genomic DNA Translation: CAA96914.1
AY692962 Genomic DNA Translation: AAT92981.1
BK006941 Genomic DNA Translation: DAA07913.1
PIRiS64220
RefSeqiNP_011313.1, NM_001181067.1

Genome annotation databases

EnsemblFungiiYGL202W; YGL202W; YGL202W
GeneIDi852672
KEGGisce:YGL202W

Similar proteinsi

Entry informationi

Entry nameiARO8_YEAST
AccessioniPrimary (citable) accession number: P53090
Secondary accession number(s): D6VTV2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 20, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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