Reviewed,
UniProtKB/Swiss-Prot P53090 (ARO8_YEAST)
Last modified
January 19, 2010.
Version 71.
History...
Clusters with 100%,
90%,
50% identity |
 Documents (3) |
 Third-party data |
 Customize display | text xml rdf/xml gff fasta |
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Aromatic amino acid aminotransferase 1 EC=2.6.1.57 Alternative name(s): Aromatic amino acid aminotransferase I Aromatic amino acid-requiring protein 8 | ||||
| Gene names |
| ||||
| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||
| Taxonomic identifier | 4932 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 500 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Has aromatic amino acid transaminase activity. Also active with methionine, alpha-aminoadipate and leucine when phenylpyruvate is the amino acceptor. Ref.1 Ref.4 |
| Catalytic activity | An aromatic amino acid + 2-oxoglutarate = an aromatic oxo acid + L-glutamate. |
| Cofactor | Pyridoxal phosphate By similarity. |
| Subcellular location | |
| Miscellaneous | Present with 1770 molecules/cell in log phase SD medium. Ref.6 |
| Sequence similarities | Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cytoplasm |
| Ligand | Pyridoxal phosphate |
| Molecular function | Aminotransferase Transferase |
| PTM | Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | aromatic amino acid family metabolic process Ref.1 Inferred from genetic interaction. Source: SGD biosynthetic processInferred from electronic annotation. Source: InterPro |
| Cellular component | cytoplasm Ref.5 Inferred from direct assay. Source: SGD |
| Molecular function | 2-aminoadipate transaminase activity Inferred from direct assay. Source: SGD aromatic-amino-acid:2-oxoglutarate aminotransferase activity Ref.4Inferred from direct assay. Source: SGD protein bindingInferred from physical interaction. Source: IntAct pyridoxal phosphate bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Binary interactions
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 500 | 500 | Aromatic amino acid aminotransferase 1 | PRO_0000064678 | |||||
Amino acid modifications | |||||||||
| Modified residue | 56 | 1 | Phosphoserine Ref.8 | ||||||
| Modified residue | 100 | 1 | Phosphoserine Ref.8 | ||||||
| Modified residue | 271 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 272 | 1 | Phosphoserine Ref.8 | ||||||
| Modified residue | 477 | 1 | Phosphoserine Ref.8 | ||||||
Sequences
| ||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "Characterisation of Saccharomyces cerevisiae ARO8 and ARO9 genes encoding aromatic aminotransferases I and II reveals a new aminotransferase subfamily." Iraqui I., Vissers S., Cartiaux M., Urrestarazu A. Mol. Gen. Genet. 257:238-248(1998) [PubMed: 9491083] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: Sigma 1278B. |
| [2] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.  Kleine K.Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679. |
| [3] | "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J.Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [4] | "Phenylalanine- and tyrosine-auxotrophic mutants of Saccharomyces cerevisiae impaired in transamination." Urrestarazu A., Vissers S., Iraqui I., Grenson M. Mol. Gen. Genet. 257:230-237(1998) [PubMed: 9491082] [Abstract] Cited for: FUNCTION. |
| [5] | "Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. |
| [6] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [7] | "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-271, MASS SPECTROMETRY. |
| [8] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-56; SER-100; SER-272 AND SER-477, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | Y13624 Genomic DNA. Translation: CAA73946.1. Z72724 Genomic DNA. Translation: CAA96914.1. AY692962 Genomic DNA. Translation: AAT92981.1. |
| PIR | S64220. |
| RefSeq | NP_011313.1. |
3D structure databases | |
| SMR | P53090. Positions 8-495. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-983N. |
| IntAct | P53090. 104 interactions. |
| STRING | P53090. |
Proteomic databases | |
| PeptideAtlas | P53090. |
| PRIDE | P53090. |
Genome annotation databases | |
| Ensembl | YGL202W; YGL202W; YGL202W; Saccharomyces cerevisiae. [Genome view] |
| GeneID | 852672. |
| KEGG | sce:YGL202W. |
| NMPDR | fig|4932.3.peg.2416. |
Organism-specific databases | |
| CYGD | YGL202w. |
| SGD | S000003170. ARO8. |
Phylogenomic databases | |
| eggNOG | fuNOG04028. |
| HOGENOM | HBG752297. |
| OMA | EWISARY. |
| OrthoDB | EOG9QC2NF. |
| PhylomeDB | P53090. |
Enzyme and pathway databases | |
| BioCyc | MetaCyc:MONOMER-11841. |
| BRENDA | 2.6.1.57. 250. |
Gene expression databases | |
| ArrayExpress | P53090. |
| Genevestigator | P53090. |
| GermOnline | YGL202W. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR004839. Aminotransferase_I/II. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view] |
| Gene3D | G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. |
| Pfam | PF00155. Aminotran_1_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 971973. |
Entry information
| Entry name | ARO8_YEAST | ||||||||
| Accession | Primary (citable) accession number: P53090 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome VII Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names |
