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Protein

Cytoplasmic tRNA 2-thiolation protein 1

Gene

NCS6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm5 tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation. May also be involved in protein urmylation and in invasive and pseudohyphal growth.UniRule annotation7 Publications

Pathwayi: 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis

This protein is involved in the pathway 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis and in tRNA modification.

GO - Molecular functioni

  • nucleotidyltransferase activity Source: UniProtKB
  • tRNA binding Source: UniProtKB

GO - Biological processi

  • protein urmylation Source: SGD
  • tRNA thio-modification Source: UniProtKB
  • tRNA wobble position uridine thiolation Source: SGD
  • tRNA wobble uridine modification Source: UniProtKB

Keywordsi

Molecular functionRNA-binding, Transferase, tRNA-binding
Biological processtRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-30688-MONOMER
UniPathwayiUPA00988

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoplasmic tRNA 2-thiolation protein 1UniRule annotation (EC:2.7.7.-UniRule annotation)
Alternative name(s):
Cytoplasmic tRNA adenylyltransferase 1UniRule annotation
Needs CLA4 to survive protein 6UniRule annotation
Thiolation of uridine in cytoplasmic tRNA protein 1UniRule annotation
Gene namesi
Name:NCS6UniRule annotation
Synonyms:CTU1UniRule annotation, TUC1UniRule annotation
Ordered Locus Names:YGL211W
ORF Names:YGL210W-A
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL211W
SGDiS000003179 NCS6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002198891 – 359Cytoplasmic tRNA 2-thiolation protein 1Add BLAST359

Proteomic databases

MaxQBiP53088
PaxDbiP53088
PRIDEiP53088

PTM databases

iPTMnetiP53088

Interactioni

Subunit structurei

Interacts with NCS2 and URM1. May act by forming a heterodimer with NCS2. Component of a large molecular weight complex of more than 250 kDa.UniRule annotation2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi33045, 303 interactors
DIPiDIP-7935N
IntActiP53088, 7 interactors
STRINGi4932.YGL211W

Structurei

3D structure databases

ProteinModelPortaliP53088
SMRiP53088
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.UniRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000001041
HOGENOMiHOG000225864
InParanoidiP53088
KOiK14168
OMAiKPLKYSY
OrthoDBiEOG092C40AW

Family and domain databases

Gene3Di3.40.50.620, 1 hit
HAMAPiMF_03053 CTU1, 1 hit
InterProiView protein in InterPro
IPR032442 CTU1_C
IPR000541 Ncs6/Tuc1/Ctu1
IPR014729 Rossmann-like_a/b/a_fold
IPR011063 TilS/TtcA_N
IPR035107 tRNA_thiolation_TtcA_Ctu1
IPR020554 UPF0021_CS
PANTHERiPTHR11807:SF12 PTHR11807:SF12, 1 hit
PfamiView protein in Pfam
PF01171 ATP_bind_3, 1 hit
PF16503 zn-ribbon_14, 1 hit
PIRSFiPIRSF004976 ATPase_YdaO, 1 hit
PROSITEiView protein in PROSITE
PS01263 UPF0021, 1 hit

Sequencei

Sequence statusi: Complete.

P53088-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFTAPSDPV NKPTKVKVSQ LCELCHSRKA LIRRPKNLSK LCKQCFCLVF
60 70 80 90 100
ETEIHNTIVA NNLFQRGEKV AVGASGGKDS TVLAHMLKLL NDRYDYGIEI
110 120 130 140 150
VLLSIDEGII GYRDDSLATV KRNQQQYGLP LEIFSFKDLY DWTMDEIVSV
160 170 180 190 200
AGIRNSCTYC GVFRRQSLDR GAAKLGISHV VTGHNADDMA ETVLMNILRG
210 220 230 240 250
DVARLEKSTA IITQSSGSPI KRSKPFKYSY QKEIVLYAHY MKLDYFSTEC
260 270 280 290 300
TYAPEAFRGT AREYMKNLEA VRPSCIIDII QSGENLALKA KKSNAGKRVV
310 320 330 340 350
KFVDGNRCAR CGYLSSNNIC KACMLLEGLE KSRAQVAIEN DTSADGAALK

LRALEKLSF
Length:359
Mass (Da):39,987
Last modified:October 3, 2006 - v3
Checksum:iF04BB5B93F642CC6
GO

Sequence cautioni

The sequence AAC49498 differs from that shown. Reason: Frameshift at position 318.Curated
The sequence CAA96927 differs from that shown. Reason: Frameshift at positions 175, 247 and 304.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti356 – 359KLSF → NSA in AAC49498 (PubMed:8813766).Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33754 Genomic DNA Translation: AAC49498.1 Frameshift.
Z72733 Genomic DNA Translation: CAA96927.1 Frameshift.
BK006941 Genomic DNA Translation: DAA07905.1
PIRiS71668 S64230
RefSeqiNP_011304.2, NM_001181076.1

Genome annotation databases

EnsemblFungiiYGL211W; YGL211W; YGL211W
GeneIDi852661
KEGGisce:YGL211W

Similar proteinsi

Entry informationi

Entry nameiCTU1_YEAST
AccessioniPrimary (citable) accession number: P53088
Secondary accession number(s): D6VTU4, Q92322
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 3, 2006
Last modified: March 28, 2018
This is version 139 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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