Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cytoplasmic tRNA 2-thiolation protein 1

Gene

NCS6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm5 tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation. May also be involved in protein urmylation and in invasive and pseudohyphal growth.UniRule annotation7 Publications

Pathwayi: 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis

This protein is involved in the pathway 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis and in tRNA modification.

GO - Molecular functioni

  • nucleotidyltransferase activity Source: UniProtKB
  • tRNA binding Source: UniProtKB

GO - Biological processi

  • protein urmylation Source: SGD
  • tRNA thio-modification Source: UniProtKB
  • tRNA wobble position uridine thiolation Source: SGD
  • tRNA wobble uridine modification Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, tRNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30688-MONOMER.
UniPathwayiUPA00988.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoplasmic tRNA 2-thiolation protein 1UniRule annotation (EC:2.7.7.-UniRule annotation)
Alternative name(s):
Cytoplasmic tRNA adenylyltransferase 1UniRule annotation
Needs CLA4 to survive protein 6UniRule annotation
Thiolation of uridine in cytoplasmic tRNA protein 1UniRule annotation
Gene namesi
Name:NCS6UniRule annotation
Synonyms:CTU1UniRule annotation, TUC1UniRule annotation
Ordered Locus Names:YGL211W
ORF Names:YGL210W-A
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL211W.
SGDiS000003179. NCS6.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002198891 – 359Cytoplasmic tRNA 2-thiolation protein 1Add BLAST359

Proteomic databases

MaxQBiP53088.
PRIDEiP53088.

PTM databases

iPTMnetiP53088.

Interactioni

Subunit structurei

Interacts with NCS2 and URM1. May act by forming a heterodimer with NCS2. Component of a large molecular weight complex of more than 250 kDa.UniRule annotation2 Publications

Protein-protein interaction databases

BioGridi33045. 129 interactors.
DIPiDIP-7935N.
IntActiP53088. 3 interactors.
MINTiMINT-4084039.

Structurei

3D structure databases

ProteinModelPortaliP53088.
SMRiP53088.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.UniRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000001041.
HOGENOMiHOG000225864.
InParanoidiP53088.
KOiK14168.
OMAiNMIEEND.
OrthoDBiEOG092C40AW.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_03053. CTU1. 1 hit.
InterProiIPR012089. 2-thiocytidine_tRNA_synth_TtcA.
IPR032442. CTU1_C.
IPR000541. Ncs6/Tuc1/Ctu1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011063. TilS/TtcA_N.
IPR020554. UPF0021_CS.
[Graphical view]
PfamiPF01171. ATP_bind_3. 1 hit.
PF16503. zn-ribbon_14. 1 hit.
[Graphical view]
PIRSFiPIRSF004976. ATPase_YdaO. 1 hit.
PROSITEiPS01263. UPF0021. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53088-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFTAPSDPV NKPTKVKVSQ LCELCHSRKA LIRRPKNLSK LCKQCFCLVF
60 70 80 90 100
ETEIHNTIVA NNLFQRGEKV AVGASGGKDS TVLAHMLKLL NDRYDYGIEI
110 120 130 140 150
VLLSIDEGII GYRDDSLATV KRNQQQYGLP LEIFSFKDLY DWTMDEIVSV
160 170 180 190 200
AGIRNSCTYC GVFRRQSLDR GAAKLGISHV VTGHNADDMA ETVLMNILRG
210 220 230 240 250
DVARLEKSTA IITQSSGSPI KRSKPFKYSY QKEIVLYAHY MKLDYFSTEC
260 270 280 290 300
TYAPEAFRGT AREYMKNLEA VRPSCIIDII QSGENLALKA KKSNAGKRVV
310 320 330 340 350
KFVDGNRCAR CGYLSSNNIC KACMLLEGLE KSRAQVAIEN DTSADGAALK

LRALEKLSF
Length:359
Mass (Da):39,987
Last modified:October 3, 2006 - v3
Checksum:iF04BB5B93F642CC6
GO

Sequence cautioni

The sequence AAC49498 differs from that shown. Reason: Frameshift at position 318.Curated
The sequence CAA96927 differs from that shown. Reason: Frameshift at positions 175, 247 and 304.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti356 – 359KLSF → NSA in AAC49498 (PubMed:8813766).Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33754 Genomic DNA. Translation: AAC49498.1. Frameshift.
Z72733 Genomic DNA. Translation: CAA96927.1. Frameshift.
BK006941 Genomic DNA. Translation: DAA07905.1.
PIRiS71668. S64230.
RefSeqiNP_011304.2. NM_001181076.1.

Genome annotation databases

EnsemblFungiiYGL211W; YGL211W; YGL211W.
GeneIDi852661.
KEGGisce:YGL211W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33754 Genomic DNA. Translation: AAC49498.1. Frameshift.
Z72733 Genomic DNA. Translation: CAA96927.1. Frameshift.
BK006941 Genomic DNA. Translation: DAA07905.1.
PIRiS71668. S64230.
RefSeqiNP_011304.2. NM_001181076.1.

3D structure databases

ProteinModelPortaliP53088.
SMRiP53088.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33045. 129 interactors.
DIPiDIP-7935N.
IntActiP53088. 3 interactors.
MINTiMINT-4084039.

PTM databases

iPTMnetiP53088.

Proteomic databases

MaxQBiP53088.
PRIDEiP53088.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL211W; YGL211W; YGL211W.
GeneIDi852661.
KEGGisce:YGL211W.

Organism-specific databases

EuPathDBiFungiDB:YGL211W.
SGDiS000003179. NCS6.

Phylogenomic databases

GeneTreeiENSGT00390000001041.
HOGENOMiHOG000225864.
InParanoidiP53088.
KOiK14168.
OMAiNMIEEND.
OrthoDBiEOG092C40AW.

Enzyme and pathway databases

UniPathwayiUPA00988.
BioCyciYEAST:G3O-30688-MONOMER.

Miscellaneous databases

PROiP53088.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_03053. CTU1. 1 hit.
InterProiIPR012089. 2-thiocytidine_tRNA_synth_TtcA.
IPR032442. CTU1_C.
IPR000541. Ncs6/Tuc1/Ctu1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011063. TilS/TtcA_N.
IPR020554. UPF0021_CS.
[Graphical view]
PfamiPF01171. ATP_bind_3. 1 hit.
PF16503. zn-ribbon_14. 1 hit.
[Graphical view]
PIRSFiPIRSF004976. ATPase_YdaO. 1 hit.
PROSITEiPS01263. UPF0021. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCTU1_YEAST
AccessioniPrimary (citable) accession number: P53088
Secondary accession number(s): D6VTU4, Q92322
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 3, 2006
Last modified: November 2, 2016
This is version 129 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.