Reviewed, UniProtKB/Swiss-Prot P53086 (KIP3_YEAST)

Names and origin

Protein names

Recommended name:
    Kinesin-like protein KIP3

Gene names

Name:	KIP3
Ordered Locus Names:	YGL216W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	805 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Miscellaneous	Present with 736 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the kinesin-like protein family. Kinesin II subfamily. Contains 1 kinesin-motor domain.

Ontologies

Keywords
Cellular component	Microtubule
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Motor protein
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	establishment of mitotic spindle orientation Inferred from mutant phenotype. Source: SGD mitotic spindle disassembly Inferred from mutant phenotype. Source: SGD mitotic spindle organization in nucleus Inferred from genetic interaction. Source: SGD nuclear migration along microtubule Inferred from mutant phenotype. Source: SGD plus-end specific microtubule depolymerization Inferred from direct assay. Source: SGD
Cellular component	cytoplasmic microtubule Inferred from direct assay. Source: SGD kinesin complex Traceable author statement. Source: SGD nuclear microtubule Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW plus-end-directed microtubule motor activity Inferred from direct assay. Source: SGD tubulin-dependent ATPase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 805	805	Kinesin-like protein KIP3		PRO_0000125404
Regions
	Domain	1 – 436	436	Kinesin-motor
	Nucleotide binding	192 – 199	8	ATP By similarity
	Coiled coil	449 – 481	33	Potential
Amino acid modifications
	Modified residue	55	1	Phosphoserine Ref.4
	Modified residue	57	1	Phosphoserine Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P53086-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 6049EF2C13D5C2C7	FASTA	805	91,090
	10 20 30 40 50 60 MNVPETRQSS IVVAIRVRPF TSMEKTRLVN EASGAEANFP GLGDSSLILP MSNNSDSDID 70 80 90 100 110 120 IDAEEGSTRS KRNSLLRRKV IRPEGIRKIV DCVDDRMLIF DPADRNPLNK VSDQVLNSMR 130 140 150 160 170 180 ARATKATASS INNSNATNKF SSQRRRHGGE IKFVFDKLFD ETSSQARVYK ETTSPLLDSV 190 200 210 220 230 240 LDGFNSTVFA YGATGCGKTY TVSGTPSQPG IIFLAMEELF NKITDLKDEK DFEISLSYLE 250 260 270 280 290 300 IYNERIRDLL KPETPSKRLV IREDTQNHIK VANLSYHHPN TVEDVMDLVV QGNINRTTSP 310 320 330 340 350 360 TEANEVSSRS HAVLQIHIMQ TNKLVDLTSQ HTFATLSIID LAGSERAAAT RNRGIRLHEG 370 380 390 400 410 420 ANINRSLLAL GNCINALCLN DGSRSCHIPY RDSKLTRLLK FSLGGNCKTV MIVCISPSSS 430 440 450 460 470 480 HYDETLNTLK YANRAKEIKT KIIRNQQSLS RHVGSYLKMI TEQKRQIEEL REREEKMISL 490 500 510 520 530 540 KLTKYKLNKE KIQLAINECV NRVQQTYAGV ETYQVAKTLK SLILCKRRFL QMVKLEVDNL 550 560 570 580 590 600 ILLFEREEST AAEMQPVISN CRMISGQLYN KIHELEMKFD ETDTLSSVIH QVHSIDLNKL 610 620 630 640 650 660 REMEDWDETY DLVYLESCLN QISELQRNEI LVNSSIMTEK LMSDPGLNSR FKFLSKWLMN 670 680 690 700 710 720 RTPNIESIIQ DLVHIDEEFE SFARTFIANP DSNFTNTNIN IINTTAADLA VPAETLQRQN 730 740 750 760 770 780 FSQKKVKWTS PDLSPSPMIE PQPELEPELH QDQDAIASEV DVSMQDTTFN EQGPSTPSAP 790 800 TTAVPRRKMR SSLLTHQSLL ATARK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII." Rieger M., Brueckner M., Schaefer M., Mueller-Auer S. Yeast 13:1077-1090(1997) [PubMed: 9290212] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-55 AND SER-57, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z72739 Genomic DNA. Translation: CAA96933.1.
PIR	S64238.
RefSeq	NP_011299.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:6383N.
IntAct	P53086. 5 interactions.
STRING	P53086.
Genome annotation databases
Ensembl	YGL216W; YGL216W; YGL216W; Saccharomyces cerevisiae. [Genome view]
GeneID	852655.
KEGG	sce:YGL216W.
NMPDR	fig|4932.3.peg.2402.
Organism-specific databases
CYGD	YGL216w.
SGD	S000003184. KIP3.
Phylogenomic databases
HOGENOM	P53086.
OMA	HAVLQIH
OrthoDB	EOG9RR80F
Gene expression databases
ArrayExpress	P53086.
Genevestigator	P53086.
GermOnline	YGL216W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001752. Kinesin_motor. IPR019821. Kinesin_motor_CS. [Graphical view]
Gene3D	G3DSA:3.40.850.10. kinesin_motor. 1 hit.
Pfam	PF00225. Kinesin. 1 hit. [Graphical view]
PRINTS	PR00380. KINESINHEAVY.
SMART	SM00129. KISc. 1 hit. [Graphical view]
PROSITE	PS00411. KINESIN_MOTOR_DOMAIN1. 1 hit. PS50067. KINESIN_MOTOR_DOMAIN2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	971933.

Entry information

Entry name

KIP3_YEAST

Accession

Primary (citable) accession number: P53086

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 24, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)