P53083 (MDM34_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial distribution and morphology protein 34

Alternative name(s):
Mitochondrial outer membrane protein MMM2

Gene names

Name:	MDM34
Synonyms:	MMM2
Ordered Locus Names:	YGL219C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	459 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the ERMES/MDM complex, which serves as a molecular tether to connect the endoplasmic reticulum and mitochondria. Components of this complex are involved in the control of mitochondrial shape and protein biogenesis and may function in phospholipid exchange. MDM34 is required for the interaction of the ER-resident membrane protein MMM1 and the outer mitochondrial membrane-resident beta-barrel protein MDM10. Ref.4 Ref.7 Ref.11
Subunit structure	Component of the ER-mitochondria encounter structure (ERMES) or MDM complex, composed of MMM1, MDM10, MDM12 and MDM34.
Subcellular location	Mitochondrion outer membrane; Multi-pass membrane protein. Note: The ERMES/MDM complex localizes to a few discrete foci (around 10 per single cell), that represent mitochondria-endoplasmic reticulum junctions. These foci are often found next to mtDNA nucleoids. Ref.5 Ref.7 Ref.8 Ref.9 Ref.11
Domain	Lacks alpha-helical transmembrane segments, suggesting that it resides in the membrane via beta-sheet conformations similar to those predicted for other outer membrane proteins and porin.
Post-translational modification	Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex containing the F-box protein MDM30. Ubiquitination is important for mitochondrial integrity. Ref.10
Miscellaneous	Present with 377 molecules/cell in log phase SD medium. Ref.6 MMM1 is required for the punctate organization of MDM34 in the mitochondrial outer membrane.
Sequence similarities	Belongs to the MDM34 family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane Transmembrane beta strand
PTM	Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	mitochondrion organization Inferred from mutant phenotype Ref.4. Source: SGD phospholipid transport Inferred from genetic interaction Ref.11. Source: SGD
Cellular component	ERMES complex Inferred from physical interaction. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RSP5	P39940	1	EBI-24154,EBI-16219

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 459	459	Mitochondrial distribution and morphology protein 34		PRO_0000096331

Sequences

	Sequence		Length	Mass (Da)
	P53083 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 222F0D0D031FC578	FASTA	459	51,990
	10 20 30 40 50 60 MSFRFNEAVF GDNSFNERIR EKLSTALNSP SKKKLDILKS GIKVQKVDFP TIPQLEILDL 70 80 90 100 110 120 DIITQPKSLA KGICKISCKD AMLRIQTVIE SNLLLINEQD TPSFTMPQLI NNGSFTIPIT 130 140 150 160 170 180 MTFSSIELEA ITNIFVKNPG IGISFNDVDL DFKFDCSVKI LQSTIERRLK ESMHVVFKDV 190 200 210 220 230 240 LPSLIFNTSQ NWFTNRGEST STIPGKREHH HQQTTMSRNV ILDGSDFQEL SPINMLRLSS 250 260 270 280 290 300 IVSSRSTLSL HSTVMNSLSA IPGCLERQNL YRFISRMPSL NNYYSSQSFP QPKSSTVSSK 310 320 330 340 350 360 QLVKPFYCSH NLLPKTVLDS SQYDLATITK IQSRLFDRSN SNDDNAKPRR RKIKCKKTRT 370 380 390 400 410 420 PSNLQSQGEQ AVDDSTAIET VTSTPVQTPI PELEEQSPPY LKTTVSIRDK YVIPEKISLN 430 440 450 LDSKKDTSKK KPFYFIGLNS QEPSNNWKWG MEDSPPPYH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII." Rieger M., Brueckner M., Schaefer M., Mueller-Auer S. Yeast 13:1077-1090(1997) [PubMed: 9290212] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Genetic basis of mitochondrial function and morphology in Saccharomyces cerevisiae." Dimmer K.S., Fritz S., Fuchs F., Messerschmitt M., Weinbach N., Neupert W., Westermann B. Mol. Biol. Cell 13:847-853(2002) [PubMed: 11907266] [Abstract] Cited for: FUNCTION.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Mmm2p, a mitochondrial outer membrane protein required for yeast mitochondrial shape and maintenance of mtDNA nucleoids." Youngman M.J., Hobbs A.E.A., Burgess S.M., Srinivasan M., Jensen R.E. J. Cell Biol. 164:677-688(2004) [PubMed: 14981098] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[8]	"Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics." Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A. J. Proteome Res. 5:1543-1554(2006) [PubMed: 16823961] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[9]	"Proteomic analysis of the yeast mitochondrial outer membrane reveals accumulation of a subclass of preproteins." Zahedi R.P., Sickmann A., Boehm A.M., Winkler C., Zufall N., Schoenfisch B., Guiard B., Pfanner N., Meisinger C. Mol. Biol. Cell 17:1436-1450(2006) [PubMed: 16407407] [Abstract] Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
[10]	"A proteomic screen reveals the mitochondrial outer membrane protein Mdm34p as an essential target of the F-box protein Mdm30p." Ota K., Kito K., Okada S., Ito T. Genes Cells 13:1075-1085(2008) [PubMed: 18775025] [Abstract] Cited for: UBIQUITINATION.
[11]	"An ER-mitochondria tethering complex revealed by a synthetic biology screen." Kornmann B., Currie E., Collins S.R., Schuldiner M., Nunnari J., Weissman J.S., Walter P. Science 325:477-481(2009) [PubMed: 19556461] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN ERMES/MDM COMPLEX, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z72741 Genomic DNA. Translation: CAA96934.1. BK006941 Genomic DNA. Translation: DAA07900.1.
PIR	S64241.
RefSeq	NP_011296.1. NM_001181084.1.
3D structure databases
ProteinModelPortal	P53083.
ModBase	Search...
Protein-protein interaction databases
IntAct	P53083. 1 interaction.
MINT	MINT-4083960.
STRING	P53083.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL219C; YGL219C; YGL219C.
GeneID	852654.
KEGG	sce:YGL219C.
NMPDR	fig|4932.3.peg.2399.
Organism-specific databases
CYGD	YGL219c.
SGD	S000003187. MDM34.
Phylogenomic databases
eggNOG	fuNOG16265.
GeneTree	EFGT00050000000348.
HOGENOM	HBG204431.
OMA	KGICKIS.
OrthoDB	EOG432473.
Gene expression databases
ArrayExpress	P53083.
Genevestigator	P53083.
GermOnline	YGL219C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	971930.

Entry information

Entry name

MDM34_YEAST

Accession

Primary (citable) accession number: P53083
Secondary accession number(s): D6VTT9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program