P53081 (NIF3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NGG1-interacting factor 3

Gene names

Name:	NIF3
Ordered Locus Names:	YGL221C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Miscellaneous	Present with 6000 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the UPF0135 (NIF3) family.

Ontologies

Keywords
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	mitochondrion Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 288	288	NGG1-interacting factor 3		PRO_0000147356
Amino acid modifications
	Modified residue	18	1	Phosphothreonine Ref.6
Experimental info
	Sequence conflict	53	1	T → A in AAT93235. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P53081 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: B6AB6E48AAF4776A	FASTA	288	31,888
	10 20 30 40 50 60 MSRAITRAQL DKLVRSITKF YPQKYADKSW DNTGLLIDCS TAQVTTADAN AKTKVLLTVD 70 80 90 100 110 120 LTKSVAQEAV DANCNVIVAY HPFIFPSWNR LSPHTNPQHE TAIKLIQYGI SVYCPHTAVD 130 140 150 160 170 180 AARGGVNDWL VRGLNNGENV AKSYALETVS GETDDLIGYG RFVEFNKDIS LEQIVKNVKR 190 200 210 220 230 240 VLRVPYVQVA SLAAPSAWNQ LKIKKVAVCA GSGSGVFKQL KEDVDLYYTG EMSHHEVLKW 250 260 270 280 KEMGKTVIVC NHSNTERGFL QDVMKGLLQD EGHEVVVSKM DCDPLTVA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII." Rieger M., Brueckner M., Schaefer M., Mueller-Auer S. Yeast 13:1077-1090(1997) [PubMed: 9290212] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-18, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z72743 Genomic DNA. Translation: CAA96937.1. AY693216 Genomic DNA. Translation: AAT93235.1. BK006941 Genomic DNA. Translation: DAA07898.1.
PIR	S64243.
RefSeq	NP_011294.1. NM_001181086.1.
3D structure databases
ProteinModelPortal	P53081.
SMR	P53081. Positions 6-287.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1469N.
IntAct	P53081. 20 interactions.
MINT	MINT-403416.
STRING	P53081.
Proteomic databases
PeptideAtlas	P53081.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL221C; YGL221C; YGL221C.
GeneID	852651.
KEGG	sce:YGL221C.
NMPDR	fig|4932.3.peg.2397.
Organism-specific databases
CYGD	YGL221c.
SGD	S000003189. NIF3.
Phylogenomic databases
eggNOG	fuNOG09512.
HOGENOM	HBG554751.
OMA	DAASQGI.
OrthoDB	EOG4CZFR8.
Gene expression databases
ArrayExpress	P53081.
Genevestigator	P53081.
GermOnline	YGL221C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002678. Interacting_NIF3. [Graphical view]
PANTHER	PTHR13799. interacting_NIF3. 1 hit.
Pfam	PF01784. NIF3. 1 hit. [Graphical view]
SUPFAM	SSF102705. interacting_NIF3. 1 hit.
TIGRFAMs	TIGR00486. YbgI_SA1388. 1 hit.
ProtoNet	Search...
Other
NextBio	971924.

Entry information

Entry name

NIF3_YEAST

Accession

Primary (citable) accession number: P53081
Secondary accession number(s): D6VVB4, E9P932

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program