Reviewed, UniProtKB/Swiss-Prot P53081 (NIF3_YEAST)

Names and origin

Protein names

Recommended name:
    NGG1-interacting factor 3

Gene names

Name:	NIF3
Ordered Locus Names:	YGL221C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Miscellaneous	Present with 6000 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the UPF0135 (NIF3) family.

Ontologies

Keywords
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	mitochondrion Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SRP1	Q02821	1	EBI-12063,EBI-1797
TEM1	P38987	1	EBI-12063,EBI-19113

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 288	288	NGG1-interacting factor 3		PRO_0000147356
Amino acid modifications
	Modified residue	18	1	Phosphothreonine Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P53081-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: B6AB6E48AAF4776A	FASTA	288	31,888
	10 20 30 40 50 60 MSRAITRAQL DKLVRSITKF YPQKYADKSW DNTGLLIDCS TAQVTTADAN AKTKVLLTVD 70 80 90 100 110 120 LTKSVAQEAV DANCNVIVAY HPFIFPSWNR LSPHTNPQHE TAIKLIQYGI SVYCPHTAVD 130 140 150 160 170 180 AARGGVNDWL VRGLNNGENV AKSYALETVS GETDDLIGYG RFVEFNKDIS LEQIVKNVKR 190 200 210 220 230 240 VLRVPYVQVA SLAAPSAWNQ LKIKKVAVCA GSGSGVFKQL KEDVDLYYTG EMSHHEVLKW 250 260 270 280 KEMGKTVIVC NHSNTERGFL QDVMKGLLQD EGHEVVVSKM DCDPLTVA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII." Rieger M., Brueckner M., Schaefer M., Mueller-Auer S. Yeast 13:1077-1090(1997) [PubMed: 9290212] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-18, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z72743 Genomic DNA. Translation: CAA96937.1.
PIR	S64243.
RefSeq	NP_011294.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1469N.
IntAct	P53081. 19 interactions.
Proteomic databases
PeptideAtlas	P53081.
Genome annotation databases
Ensembl	YGL221C. Saccharomyces cerevisiae. [Contig view]
GeneID	852651.
GenomeReviews	Gene locus YGL221C in contig Y13135_GR.
KEGG	sce:YGL221C.
NMPDR	fig|4932.3.peg.2397.
Organism-specific databases
CYGD	YGL221c.
SGD	S000003189. NIF3.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P53081.
OMA	P53081. DAASQGI.
Gene expression databases
GermOnline	YGL221C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002678. Interacting_NIF3. [Graphical view]
PANTHER	PTHR13799. interacting_NIF3. 1 hit.
Pfam	PF01784. NIF3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00486. interacting_NIF3. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	971924.

Entry information

Entry name

NIF3_YEAST

Accession

Primary (citable) accession number: P53081

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)