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Protein

Vacuolar import and degradation protein 30

Gene

VID30

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a role in the negative regulation of gluconeogenesis. Required for both proteasome-dependent and vacuolar catabolite degradation of fructose-1,6-bisphosphatase (FBPase).1 Publication

GO - Biological processi

  • negative regulation of gluconeogenesis Source: SGD
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: SGD
  • regulation of nitrogen utilization Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-30701-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar import and degradation protein 30
Alternative name(s):
Glucose-induced degradation protein 1
Gene namesi
Name:VID30
Synonyms:GID1
Ordered Locus Names:YGL227W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL227W.
SGDiS000003196. VID30.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • GID complex Source: SGD
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 958957Vacuolar import and degradation protein 30PRO_0000065829Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources
Modified residuei147 – 1471PhosphoserineCombined sources
Modified residuei246 – 2461PhosphoserineCombined sources
Modified residuei248 – 2481PhosphoserineCombined sources
Modified residuei277 – 2771PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP53076.
PeptideAtlasiP53076.

PTM databases

iPTMnetiP53076.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
GID7P255695EBI-24173,EBI-21727
GID8P4020814EBI-24173,EBI-27276
RMD5Q125088EBI-24173,EBI-38868
VID28P4054710EBI-24173,EBI-24957

Protein-protein interaction databases

BioGridi33012. 64 interactions.
DIPiDIP-6352N.
IntActiP53076. 18 interactions.
MINTiMINT-692085.

Structurei

3D structure databases

ProteinModelPortaliP53076.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini390 – 592203B30.2/SPRYPROSITE-ProRule annotationAdd
BLAST
Domaini710 – 74233LisHPROSITE-ProRule annotationAdd
BLAST
Domaini769 – 82658CTLHPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi165 – 18521Poly-AspAdd
BLAST
Compositional biasi450 – 4534Poly-Ser

Sequence similaritiesi

Contains 1 B30.2/SPRY domain.PROSITE-ProRule annotation
Contains 1 CTLH domain.PROSITE-ProRule annotation
Contains 1 LisH domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00530000063160.
HOGENOMiHOG000000917.
InParanoidiP53076.
OMAiINDYLIH.
OrthoDBiEOG7T1RKP.

Family and domain databases

InterProiIPR001870. B30.2/SPRY.
IPR013320. ConA-like_dom.
IPR013144. CRA_dom.
IPR024964. CTLH/CRA.
IPR006595. CTLH_C.
IPR006594. LisH.
IPR003877. SPRY_dom.
[Graphical view]
PfamiPF10607. CLTH. 1 hit.
PF00622. SPRY. 1 hit.
[Graphical view]
SMARTiSM00757. CRA. 1 hit.
SM00668. CTLH. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS50897. CTLH. 1 hit.
PS50896. LISH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53076-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEYMDDVDR EFINCLFPSY LLQQPVAYDL WILYLQHRKL FHKLKNTNLI
60 70 80 90 100
NADENPTGVG MGRTKLTALT RKEIWSKLMN LGVLGTISFE AVNDDYLIQV
110 120 130 140 150
YKYFYPDVND FTLRFGVKDS NKNSVRVMKA SSDMRKNAQE LLEPVLSERE
160 170 180 190 200
MALNSNTSLE NDRNDDDDDD DDDDDDDDDD DDDDDESDLE SLEGEVDTDT
210 220 230 240 250
DDNNEGDGSD NHEEGGEEGS RGADADVSSA QQRAERVADP WIYQRSRSAI
260 270 280 290 300
NIETESRNLW DTSDKNSGLQ YYPPDQSPSS SFSSPRVSSG NDKNDNEATN
310 320 330 340 350
VLSNSGSKKK NSMIPDIYKI LGYFLPSRWQ AQPNNSLQLS QDGITHLQPN
360 370 380 390 400
PDYHSYMTYE RSSASSASTR NRLRTSFENS GKVDFAVTWA NKSLPDNKLT
410 420 430 440 450
IFYYEIKVLS VTSTESAENS NIVIGYKLVE NELMEATTKK SVSRSSVAGS
460 470 480 490 500
SSSLGGSNNM SSNRVPSTSF TMEGTQRRDY IYEGGVSAMS LNVDGSINKC
510 520 530 540 550
QKYGFDLNVF GYCGFDGLIT NSTEQSKEYA KPFGRDDVIG CGINFIDGSI
560 570 580 590 600
FFTKNGIHLG NAFTDLNDLE FVPYVALRPG NSIKTNFGLN EDFVFDIIGY
610 620 630 640 650
QDKWKSLAYE HICRGRQMDV SIEEFDSDES EEDETENGPE ENKSTNVNED
660 670 680 690 700
LMDIDQEDGA AGNKDTKKLN DEKDNNLKFL LGEDNRFIDG KLVRPDVNNI
710 720 730 740 750
NNLSVDDGSL PNTLNVMIND YLIHEGLVDV AKGFLKDLQK DAVNVNGQHS
760 770 780 790 800
ESKDVIRHNE RQIMKEERMV KIRQELRYLI NKGQISKCIN YIDNEIPDLL
810 820 830 840 850
KNNLELVFEL KLANYLVMIK KSSSKDDDEI ENLILKGQEL SNEFIYDTKI
860 870 880 890 900
PQSLRDRFSG QLSNVSALLA YSNPLVEAPK EISGYLSDEY LQERLFQVSN
910 920 930 940 950
NTILTFLHKD SECALENVIS NTRAMLSTLL EYNAFGSTNS SDPRYYKAIN

FDEDVLNL
Length:958
Mass (Da):108,179
Last modified:October 1, 1996 - v1
Checksum:i335ADD152949F8C8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72749 Genomic DNA. Translation: CAA96943.1.
BK006941 Genomic DNA. Translation: DAA07891.1.
PIRiS64249.
RefSeqiNP_011287.1. NM_001181093.1.

Genome annotation databases

EnsemblFungiiYGL227W; YGL227W; YGL227W.
GeneIDi852624.
KEGGisce:YGL227W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72749 Genomic DNA. Translation: CAA96943.1.
BK006941 Genomic DNA. Translation: DAA07891.1.
PIRiS64249.
RefSeqiNP_011287.1. NM_001181093.1.

3D structure databases

ProteinModelPortaliP53076.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33012. 64 interactions.
DIPiDIP-6352N.
IntActiP53076. 18 interactions.
MINTiMINT-692085.

PTM databases

iPTMnetiP53076.

Proteomic databases

MaxQBiP53076.
PeptideAtlasiP53076.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL227W; YGL227W; YGL227W.
GeneIDi852624.
KEGGisce:YGL227W.

Organism-specific databases

EuPathDBiFungiDB:YGL227W.
SGDiS000003196. VID30.

Phylogenomic databases

GeneTreeiENSGT00530000063160.
HOGENOMiHOG000000917.
InParanoidiP53076.
OMAiINDYLIH.
OrthoDBiEOG7T1RKP.

Enzyme and pathway databases

BioCyciYEAST:G3O-30701-MONOMER.

Miscellaneous databases

NextBioi971844.
PROiP53076.

Family and domain databases

InterProiIPR001870. B30.2/SPRY.
IPR013320. ConA-like_dom.
IPR013144. CRA_dom.
IPR024964. CTLH/CRA.
IPR006595. CTLH_C.
IPR006594. LisH.
IPR003877. SPRY_dom.
[Graphical view]
PfamiPF10607. CLTH. 1 hit.
PF00622. SPRY. 1 hit.
[Graphical view]
SMARTiSM00757. CRA. 1 hit.
SM00668. CTLH. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS50897. CTLH. 1 hit.
PS50896. LISH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Catabolite degradation of fructose-1,6-bisphosphatase in the yeast Saccharomyces cerevisiae: a genome-wide screen identifies eight novel GID genes and indicates the existence of two degradation pathways."
    Regelmann J., Schuele T., Josupeit F.S., Horak J., Rose M., Entian K.-D., Thumm M., Wolf D.H.
    Mol. Biol. Cell 14:1652-1663(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-147; SER-246 AND SER-248, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-147; SER-248 AND SER-277, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiVID30_YEAST
AccessioniPrimary (citable) accession number: P53076
Secondary accession number(s): D6VVA7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 11, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1500 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.