P53075 (SHE10_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sensitivity to high expression protein 10

Gene names

Name:	SHE10
Ordered Locus Names:	YGL228W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	577 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the mitochondrial RNase MRP (MtMRP), a ribonucleoprotein endoribonuclease involved in the cleaving RNA transcripts to generate primers for DNA replication in mitochondria. Absent in the nucleus-localized RNase MRP (NuMRP). Causes growth arrest when highly overexpressed. Ref.3
Subunit structure	Component of the mitochondria-localized RNase mitochondrial RNA-processing (RNase MRP) composed of one single RNA encoded by the NME1 gene and at least 31 proteins.
Subcellular location	Mitochondrion Ref.5.
Miscellaneous	Present with 8640 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the SHE10 family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Coiled coil
Molecular function	Ribonucleoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell ribonucleoprotein complex Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
YHR080C	P38800	1	EBI-24178,EBI-24597

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 577	577	Sensitivity to high expression protein 10		PRO_0000202713
Regions
	Coiled coil	379 – 416	38	Potential
	Coiled coil	513 – 562	50	Potential

Sequences

	Sequence		Length	Mass (Da)
	P53075 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: EF4CA95FA3157660	FASTA	577	66,862
	10 20 30 40 50 60 MGKLIKLITT LTVLVSLLQY CCEFNSGSIS CERTQTLCHY TNPRVWNTYF SRNCELYKNK 70 80 90 100 110 120 VSPGFDIVAR KYDTAVKPVI DDATVKVNKV AIQPAFKVIH SQCKKWNCGK YYQLVRSPMV 130 140 150 160 170 180 KTRRFFFAKY NAFVKPNLDK FFTAEFRSHL KERILKYKNI GHYYFTITSR CIKSKYDFIV 190 200 210 220 230 240 GNTEEKLMGK FKNKDTHGIH GSVTREPSSE DMVLTVSTME SDEEELTTTS TQTVVETITL 250 260 270 280 290 300 DQEEASAVAN HAHDDEASTD VEGSTDVNVN EQALLQEDFD MWSETILQKT QDVIQLFEKD 310 320 330 340 350 360 VSKYINGKLV EEANHFKAKF QSLDDKSKKF FSKISLAIND IECVEGIDSE TGKKIFFDKS 370 380 390 400 410 420 GSTEISQYIT RELVREYFNE TRSTLDELTN AMEKDLSEIT DEIEKKVNAI REENVEVFEE 430 440 450 460 470 480 WGDIIVNEWS KRMAYVDVIN AHMGADDDTT LDEEKAKSSV NWKKFLKGKK QIIESRDKLA 490 500 510 520 530 540 HHSADLSRVN AFRQKVQKKI LSFTQESGEF LYILRSKANL QFQERERKER ERKEREKAAA 550 560 570 EEFQRQQELL RQQEEEDEED VSYTSTSTIT TTITMTL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"An efficient method to isolate yeast genes causing overexpression-mediated growth arrest." Espinet C., de la Torre M.A., Aldea M., Herrero E. Yeast 11:25-32(1995) [PubMed: 7762298] [Abstract] Cited for: FUNCTION.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Comparison of mitochondrial and nucleolar RNase MRP reveals identical RNA components with distinct enzymatic activities and protein components." Lu Q., Wierzbicki S., Krasilnikov A.S., Schmitt M.E. RNA 16:529-537(2010) [PubMed: 20086051] [Abstract] Cited for: IDENTIFICATION IN MITOCHONDRIAL RNASE MRP, SUBCELLULAR LOCATION, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z72750 Genomic DNA. Translation: CAA96945.1. BK006941 Genomic DNA. Translation: DAA07890.1.
PIR	S64250.
RefSeq	NP_011286.1. NM_001181094.1.
3D structure databases
ProteinModelPortal	P53075.
ModBase	Search...
Protein-protein interaction databases
IntAct	P53075. 3 interactions.
Proteomic databases
PeptideAtlas	P53075.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL228W; YGL228W; YGL228W.
GeneID	852623.
KEGG	sce:YGL228W.
NMPDR	fig|4932.3.peg.2389.
Organism-specific databases
CYGD	YGL228w.
SGD	S000003197. SHE10.
Phylogenomic databases
eggNOG	fuNOG09181.
GeneTree	EFGT00050000008673.
OMA	FEEWGDI.
OrthoDB	EOG4QVGNG.
Gene expression databases
ArrayExpress	P53075.
Genevestigator	P53075.
GermOnline	YGL228W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	971841.

Entry information

Entry name

SHE10_YEAST

Accession

Primary (citable) accession number: P53075
Secondary accession number(s): D6VVA6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program