P53073 (EMC4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ER membrane protein complex subunit 4

Gene names

Name:	EMC4
Ordered Locus Names:	YGL231C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	190 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The EMC seems to be required for efficient folding of proteins in the endoplasmic reticulum (ER).
Subunit structure	Component of the ER membrane protein complex (EMC), which is composed of EMC1, EMC2, EMC3, EMC4, EMC5 and EMC6. Ref.7
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.4.
Miscellaneous	Present with 1320 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the EMC4 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein folding in endoplasmic reticulum Inferred from genetic interaction Ref.7. Source: SGD
Cellular_component	ER membrane protein complex Inferred from direct assay Ref.7. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 190	190	ER membrane protein complex subunit 4		PRO_0000202711
Regions
	Transmembrane	98 – 118	21	Helical; Potential
	Transmembrane	136 – 156	21	Helical; Potential
Amino acid modifications
	Modified residue	16	1	Phosphothreonine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P53073 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 062AE1EAB05360EE	FASTA	190	21,460
	10 20 30 40 50 60 MSEQEPYEWA KHLLDTKYIE KYNIQNSNTL PSPPGFEGNS SKGNVTRKQQ DATSQTTSLA 70 80 90 100 110 120 QKNQITVLQV QKAWQIALQP AKSIPMNIFM SYMSGTSLQI IPIMTALMLL SGPIKAIFST 130 140 150 160 170 180 RSAFKPVLGN KATQSQVQTA MFMYIVFQGV LMYIGYRKLN SMGLIPNAKG DWLPWERIAH 190 YNNGLQWFSD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-16, MASS SPECTROMETRY. Strain: YAL6B.
[7]	"Comprehensive characterization of genes required for protein folding in the endoplasmic reticulum." Jonikas M.C., Collins S.R., Denic V., Oh E., Quan E.M., Schmid V., Weibezahn J., Schwappach B., Walter P., Weissman J.S., Schuldiner M. Science 323:1693-1697(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN EMC COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z72753 Genomic DNA. Translation: CAA96949.1. AY558267 Genomic DNA. Translation: AAS56593.1. BK006941 Genomic DNA. Translation: DAA07887.1.
PIR	S64253.
RefSeq	NP_011283.1. NM_001181097.1.
3D structure databases
ProteinModelPortal	P53073.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4486491.
STRING	4932.YGL231C.
Proteomic databases
PaxDb	P53073.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL231C; YGL231C; YGL231C.
GeneID	852620.
KEGG	sce:YGL231C.
Organism-specific databases
CYGD	YGL231c.
SGD	S000003200. EMC4.
Phylogenomic databases
eggNOG	NOG308755.
GeneTree	ENSGT00390000006970.
HOGENOM	HOG000163282.
OMA	RSADQHE.
OrthoDB	EOG4PCF2G.
Enzyme and pathway databases
BioCyc	YEAST:G3O-30705-MONOMER.
Gene expression databases
Genevestigator	P53073.
GermOnline	YGL231C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR009445. DUF1077_TMEM85. [Graphical view]
PANTHER	PTHR19315. PTHR19315. 1 hit.
Pfam	PF06417. DUF1077. 1 hit. [Graphical view]
PIRSF	PIRSF017207. UCP017207_TM-p85. 1 hit.
ProtoNet	Search...
Other
NextBio	971832.

Entry information

Entry name

EMC4_YEAST

Accession

Primary (citable) accession number: P53073
Secondary accession number(s): D6VVA3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 29, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program