Reviewed, UniProtKB/Swiss-Prot P53073 (EMC4_YEAST)

Names and origin

Protein names

Recommended name:
    ER membrane protein complex subunit 4

Gene names

Name:	EMC4
Ordered Locus Names:	YGL231C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	190 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The EMC seems to be required for efficient folding of proteins in the endoplasmic reticulum (ER).
Subunit structure	Component of the ER membrane protein complex (EMC), which is composed of EMC1, EMC2, EMC3, EMC4, EMC5 and EMC6.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Miscellaneous	Present with 1320 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the TMEM85 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein folding in endoplasmic reticulum Inferred from genetic interaction. Source: SGD
Cellular component	endoplasmic reticulum Ref.3 Inferred from direct assay. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 190	190	ER membrane protein complex subunit 4		PRO_0000202711
Regions
	Transmembrane	98 – 118	21	Potential
	Transmembrane	136 – 156	21	Potential
Amino acid modifications
	Modified residue	16	1	Phosphothreonine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P53073-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 062AE1EAB05360EE	FASTA	190	21,460
	10 20 30 40 50 60 MSEQEPYEWA KHLLDTKYIE KYNIQNSNTL PSPPGFEGNS SKGNVTRKQQ DATSQTTSLA 70 80 90 100 110 120 QKNQITVLQV QKAWQIALQP AKSIPMNIFM SYMSGTSLQI IPIMTALMLL SGPIKAIFST 130 140 150 160 170 180 RSAFKPVLGN KATQSQVQTA MFMYIVFQGV LMYIGYRKLN SMGLIPNAKG DWLPWERIAH 190 YNNGLQWFSD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-16, MASS SPECTROMETRY.
[6]	"Comprehensive characterization of genes required for protein folding in the endoplasmic reticulum." Jonikas M.C., Collins S.R., Denic V., Oh E., Quan E.M., Schmid V., Weibezahn J., Schwappach B., Walter P., Weissman J.S., Schuldiner M. Science 323:1693-1697(2009) [PubMed: 19325107] [Abstract] Cited for: IDENTIFICATION IN EMC COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z72753 Genomic DNA. Translation: CAA96949.1. AY558267 Genomic DNA. Translation: AAS56593.1.
PIR	S64253.
RefSeq	NP_011283.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P53073.
Genome annotation databases
Ensembl	YGL231C; YGL231C; YGL231C; Saccharomyces cerevisiae. [Genome view]
GeneID	852620.
KEGG	sce:YGL231C.
NMPDR	fig|4932.3.peg.2386.
Organism-specific databases
CYGD	YGL231c.
SGD	S000003200. EMC4.
Phylogenomic databases
HOGENOM	P53073.
OMA	RSADQHE
OrthoDB	EOG9V44QS
Gene expression databases
ArrayExpress	P53073.
Genevestigator	P53073.
GermOnline	YGL231C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR009445. DUF1077. IPR016687. UCP017207_TM-p85. [Graphical view]
PANTHER	PTHR19315. DUF1077. 1 hit.
Pfam	PF06417. DUF1077. 1 hit. [Graphical view]
PIRSF	PIRSF017207. UCP017207_TM-p85. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	971832.

Entry information

Entry name

EMC4_YEAST

Accession

Primary (citable) accession number: P53073

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 24, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)