P53070 (MTO1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial translation optimization protein 1

Gene names

Name:	MTO1
Synonyms:	IPS1
Ordered Locus Names:	YGL236C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	669 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the 5-carboxymethylaminomethyl modification (mnm5s2U34) of the wobble uridine base in mitochondrial tRNAs.
Cofactor	FAD By similarity.
Subunit structure	Forms an heterodimer with MSS1.
Subcellular location	Mitochondrion Ref.3.
Sequence similarities	Belongs to the MnmG family.
Sequence caution	The sequence CAA96953.1 differs from that shown. Reason: Frameshift at position 663.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	FAD Flavoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	mitochondrial tRNA wobble uridine modification Inferred from mutant phenotype. Source: SGD
Cellular component	mitochondrion Inferred from direct assay Ref.3. Source: SGD
Molecular function	flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 59	59	Mitochondrion Potential
	Chain	60 – 669	610	Mitochondrial translation optimization protein 1		PRO_0000042687

Sequences

	Sequence		Length	Mass (Da)
	P53070 [UniParc]. Last modified October 3, 2006. Version 2. Checksum: 917F22AE324F1B87	FASTA	669	74,215
	10 20 30 40 50 60 MLRVTTLASS CTSFPLQVLR RRLTISSLTS FQPTTKTQVV VIGAGHAGCE AAAASSRTGA 70 80 90 100 110 120 HTTLITPSLT DIGKCSCNPS IGGVGKGILV KEIDALDGLM GKVTDLAGVQ FKMLNRSKGP 130 140 150 160 170 180 AVWGPRAQID RELYKKYMQR ELSDKKAHPN LSLLQNKVAD LILYDPGCGH KVIKGVVLDD 190 200 210 220 230 240 GTQVGADQVI ITTGTFLSAE IHIGDKRIAA GRIGEQPTYG ISNTLQNEVG FQLGRLKTGT 250 260 270 280 290 300 PARLAKESID FSALEVQKGD ALPVPMSFLN ETVSVEPTKQ LDCFGTHTTP QMHDFLRNNL 310 320 330 340 350 360 HQSIHIQDTT IKGPRYCPSI EAKILRFPDR SSHKIWLEPE GFNSDVIYPN GISNSMPEDV 370 380 390 400 410 420 QLQMMRLIPG MANVEILQPA YGVEYDYVDP RQLKPSLETK LVDGLFLAGQ INGTTGYEEA 430 440 450 460 470 480 AAQGIIAGIN AGLLSRQERE QLVLKRSEAY IGVLIDDLIN NGVIEPYRMF TSRSEFRISV 490 500 510 520 530 540 RADNADFRLT PIGAQLGIIS PVRLSQYSRD KHLYDETIRA LQNFKLSSQK WSSLLQANIA 550 560 570 580 590 600 PQAENRSAWE IFRFKDMDLH KLYECIPDLP INLLDIPMHV VTKINIQGKY EPYIVKQNQF 610 620 630 640 650 660 VKAFQADENM LLPQDYDYRQ LPTLSTECKL LLNRVQPLTI GQARRIQGIT AAALFELYRV ARKPSQPVM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"MTO1 codes for a mitochondrial protein required for respiration in paromomycin-resistant mutants of Saccharomyces cerevisiae." Colby G., Wu M., Tzagoloff A. J. Biol. Chem. 273:27945-27952(1998) [PubMed: 9774408] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH MSS1.
[4]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed: 12748633] [Abstract] Cited for: IDENTIFICATION OF FRAMESHIFT.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z72758 Genomic DNA. Translation: CAA96953.1. Frameshift. BK006941 Genomic DNA. Translation: DAA07882.1.
PIR	S64258.
RefSeq	NP_011278.2. NM_001181102.1.
3D structure databases
ProteinModelPortal	P53070.
SMR	P53070. Positions 32-663.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4488N.
IntAct	P53070. 5 interactions.
MINT	MINT-535976.
STRING	P53070.
Proteomic databases
PeptideAtlas	P53070.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL236C; YGL236C; YGL236C.
GeneID	852616.
KEGG	sce:YGL236C.
NMPDR	fig|4932.3.peg.2381.
Organism-specific databases
CYGD	YGL236c.
SGD	S000003205. MTO1.
Phylogenomic databases
eggNOG	fuNOG04380.
GeneTree	EFGT00050000003642.
HOGENOM	HBG284774.
OMA	IHIGERQ.
OrthoDB	EOG42C1HS.
Gene expression databases
ArrayExpress	P53070.
Genevestigator	P53070.
GermOnline	YGL236C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004416. GidA. IPR002218. GIDA-rel. IPR020595. GIDA-rel_CS. [Graphical view]
KO	K03495.
Pfam	PF01134. GIDA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00136. GidA. 1 hit.
PROSITE	PS01280. GIDA_1. 1 hit. PS01281. GIDA_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971820.

Entry information

Entry name

MTO1_YEAST

Accession

Primary (citable) accession number: P53070
Secondary accession number(s): D6VV98

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 3, 2006
Last modified:	December 14, 2011
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program