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Protein

Mitochondrial translation optimization protein 1

Gene

MTO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the 5-carboxymethylaminomethyl modification (mnm5s2U34) of the wobble uridine base in mitochondrial tRNAs.

Cofactori

FADBy similarity

GO - Molecular functioni

GO - Biological processi

  • mitochondrial tRNA wobble uridine modification Source: SGD
  • tRNA modification Source: Reactome
Complete GO annotation...

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciYEAST:G3O-30709-MONOMER.
ReactomeiR-SCE-6787450. tRNA modification in the mitochondrion.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial translation optimization protein 1
Gene namesi
Name:MTO1
Synonyms:IPS1
Ordered Locus Names:YGL236C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL236C.
SGDiS000003205. MTO1.

Subcellular locationi

  • Mitochondrion 1 Publication

GO - Cellular componenti

  • mitochondrial matrix Source: Reactome
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi431 – 4311A → T: Does not completely restore mitochondrial DNA-dependent respiratory chain activities in respiration-defective mutant cells. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5959MitochondrionSequence analysisAdd
BLAST
Chaini60 – 669610Mitochondrial translation optimization protein 1PRO_0000042687Add
BLAST

Proteomic databases

MaxQBiP53070.

Interactioni

Subunit structurei

Forms a heterodimer with MSS1.

Protein-protein interaction databases

BioGridi33004. 79 interactions.
DIPiDIP-4488N.
IntActiP53070. 3 interactions.
MINTiMINT-535976.

Structurei

3D structure databases

ProteinModelPortaliP53070.
SMRiP53070. Positions 39-657.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000011297.
HOGENOMiHOG000201059.
InParanoidiP53070.
KOiK03495.
OMAiTRSDNAD.
OrthoDBiEOG092C1TLA.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_00129. MnmG_GidA. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR026904. GidA-assoc_3.
IPR004416. MnmG.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
PF13932. GIDA_assoc. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
TIGRFAMsiTIGR00136. gidA. 1 hit.
PROSITEiPS01280. GIDA_1. 1 hit.
PS01281. GIDA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53070-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRVTTLASS CTSFPLQVLR RRLTISSLTS FQPTTKTQVV VIGAGHAGCE
60 70 80 90 100
AAAASSRTGA HTTLITPSLT DIGKCSCNPS IGGVGKGILV KEIDALDGLM
110 120 130 140 150
GKVTDLAGVQ FKMLNRSKGP AVWGPRAQID RELYKKYMQR ELSDKKAHPN
160 170 180 190 200
LSLLQNKVAD LILYDPGCGH KVIKGVVLDD GTQVGADQVI ITTGTFLSAE
210 220 230 240 250
IHIGDKRIAA GRIGEQPTYG ISNTLQNEVG FQLGRLKTGT PARLAKESID
260 270 280 290 300
FSALEVQKGD ALPVPMSFLN ETVSVEPTKQ LDCFGTHTTP QMHDFLRNNL
310 320 330 340 350
HQSIHIQDTT IKGPRYCPSI EAKILRFPDR SSHKIWLEPE GFNSDVIYPN
360 370 380 390 400
GISNSMPEDV QLQMMRLIPG MANVEILQPA YGVEYDYVDP RQLKPSLETK
410 420 430 440 450
LVDGLFLAGQ INGTTGYEEA AAQGIIAGIN AGLLSRQERE QLVLKRSEAY
460 470 480 490 500
IGVLIDDLIN NGVIEPYRMF TSRSEFRISV RADNADFRLT PIGAQLGIIS
510 520 530 540 550
PVRLSQYSRD KHLYDETIRA LQNFKLSSQK WSSLLQANIA PQAENRSAWE
560 570 580 590 600
IFRFKDMDLH KLYECIPDLP INLLDIPMHV VTKINIQGKY EPYIVKQNQF
610 620 630 640 650
VKAFQADENM LLPQDYDYRQ LPTLSTECKL LLNRVQPLTI GQARRIQGIT
660
AAALFELYRV ARKPSQPVM
Length:669
Mass (Da):74,215
Last modified:October 3, 2006 - v2
Checksum:i917F22AE324F1B87
GO

Sequence cautioni

The sequence CAA96953 differs from that shown. Reason: Frameshift at position 663. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72758 Genomic DNA. Translation: CAA96953.1. Frameshift.
BK006941 Genomic DNA. Translation: DAA07882.1.
PIRiS64258.
RefSeqiNP_011278.2. NM_001181102.1.

Genome annotation databases

EnsemblFungiiYGL236C; YGL236C; YGL236C.
GeneIDi852616.
KEGGisce:YGL236C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72758 Genomic DNA. Translation: CAA96953.1. Frameshift.
BK006941 Genomic DNA. Translation: DAA07882.1.
PIRiS64258.
RefSeqiNP_011278.2. NM_001181102.1.

3D structure databases

ProteinModelPortaliP53070.
SMRiP53070. Positions 39-657.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33004. 79 interactions.
DIPiDIP-4488N.
IntActiP53070. 3 interactions.
MINTiMINT-535976.

Proteomic databases

MaxQBiP53070.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL236C; YGL236C; YGL236C.
GeneIDi852616.
KEGGisce:YGL236C.

Organism-specific databases

EuPathDBiFungiDB:YGL236C.
SGDiS000003205. MTO1.

Phylogenomic databases

GeneTreeiENSGT00390000011297.
HOGENOMiHOG000201059.
InParanoidiP53070.
KOiK03495.
OMAiTRSDNAD.
OrthoDBiEOG092C1TLA.

Enzyme and pathway databases

BioCyciYEAST:G3O-30709-MONOMER.
ReactomeiR-SCE-6787450. tRNA modification in the mitochondrion.

Miscellaneous databases

PROiP53070.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_00129. MnmG_GidA. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR026904. GidA-assoc_3.
IPR004416. MnmG.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
PF13932. GIDA_assoc. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
TIGRFAMsiTIGR00136. gidA. 1 hit.
PROSITEiPS01280. GIDA_1. 1 hit.
PS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMTO1_YEAST
AccessioniPrimary (citable) accession number: P53070
Secondary accession number(s): D6VV98
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 3, 2006
Last modified: September 7, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.