Reviewed, UniProtKB/Swiss-Prot P53067 (IMB5_YEAST)

Names and origin

Protein names

Recommended name:
    Importin subunit beta-5
Alternative name(s):
    Karyopherin subunit beta-5
    Karyopherin-114
    114 kDa karyopherin

Gene names

Name:	KAP114
Ordered Locus Names:	YGL241W
ORF Names:	HRC1004

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1004 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for nuclear protein import and mediates docking of import substrate to distinct nucleoporins. Serves a receptor for nuclear localization signals. Mediates the nuclear import of TATA-binding protein (TBP) and of histones H2A and H2B. Ref.3 Ref.4
Subunit structure	Interacts with NAP1. Ref.7
Subcellular location	Cytoplasm. Nucleus › nuclear pore complex.
Miscellaneous	Present with 2940 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the importin beta family. Contains 1 importin N-terminal domain.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport mRNA transport
Cellular component	Cytoplasm Nuclear pore complex Nucleus
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	mRNA transport Inferred from electronic annotation. Source: UniProtKB-KW protein import into nucleus Inferred from mutant phenotype. Source: SGD transmembrane transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nuclear pore Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein transmembrane transporter activity Inferred from physical interaction. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1004	1004	Importin subunit beta-5		PRO_0000120778
Regions
	Domain	21 – 100	80	Importin N-terminal

Sequences

	Sequence		Length	Mass (Da)
	P53067-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 1F4C02AA6AB2FF39	FASTA	1,004	113,921
	10 20 30 40 50 60 MDINELIIGA QSADKHTREV AETQLLQWCD SDASQVFKAL ANVALQHEAS LESRQFALLS 70 80 90 100 110 120 LRKLITMYWS PGFESYRSTS NVEIDVKDFI REVLLKLCLN DNENTKIKNG ASYCIVQISA 130 140 150 160 170 180 VDFPDQWPQL LTVIYDAISH QHSLNAMSLL NEIYDDVVSE EMFFEGGIGL ATMEIVFKVL 190 200 210 220 230 240 NTETSTLIAK IAALKLLKAC LLQMSSHNEY DEASRKSFVS QCLATSLQIL GQLLTLNFGN 250 260 270 280 290 300 VDVISQLKFK SIIYENLVFI KNDFSRKHFS SELQKQFKIM AIQDLENVTH INANVETTES 310 320 330 340 350 360 EPLLETVHDC SIYIVEFLTS VCTLQFSVEE MNKIITSLTI LCQLSSETRE IWTSDFNTFV 370 380 390 400 410 420 SKETGLAASY NVRDQANEFF TSLPNPQLSL IFKVVSNDIE HSTCNYSTLE SLLYLLQCIL 430 440 450 460 470 480 LNDDEITGEN IDQSLQILIK TLENILVSQE IPELILARAI LTIPRVLDKF IDALPDIKPL 490 500 510 520 530 540 TSAFLAKSLN LALKSDKELI KSATLIAFTY YCYFAELDSV LGPEVCSETQ EKVIRIINQV 550 560 570 580 590 600 SSDAEEDTNG ALMEVLSQVI SYNPKEPHSR KEILQAEFHL VFTISSEDPA NVQVVVQSQE 610 620 630 640 650 660 CLEKLLDNIN MDNYKNYIEL CLPSFINVLD SNNANNYRYS PLLSLVLEFI TVFLKKKPND 670 680 690 700 710 720 GFLPDEINQY LFEPLAKVLA FSTEDETLQL ATEAFSYLIF NTDTRAMEPR LMDIMKVLER 730 740 750 760 770 780 LLSLEVSDSA AMNVGPLVVA IFTRFSKEIQ PLIGRILEAV VVRLIKTQNI STEQNLLSVL 790 800 810 820 830 840 CFLTCNDPKQ TVDFLSSFQI DNTDALTLVM RKWIEAFEVI RGEKRIKENI VALSNLFFLN 850 860 870 880 890 900 DKRLQKVVVN GNLIPYEGDL IITRSMAKKM PDRYVQVPLY TKIIKLFVSE LSFQSKQPNP 910 920 930 940 950 960 EQLITSDIKQ EVVNANKDDD NDDWEDVDDV LDYDKLKEYI DDDVDEEADD DSDDITGLMD 970 980 990 1000 VKESVVQLLV RFFKEVASKD VSGFHCIYET LSDSERKVLS EALL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of an 11.1 kb DNA fragment between ADH4 and ADE5 on the left arm of chromosome VII, reveals the presence of eight open reading frames." Vandenbol M., Durand P., Portetelle D., Hilger F. Yeast 11:1519-1523(1995) [PubMed: 8750240] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The importin/karyopherin Kap114 mediates the nuclear import of TATA-binding protein." Morehouse H., Buratowski R.M., Silver P.A., Buratowski S. Proc. Natl. Acad. Sci. U.S.A. 96:12542-12547(1999) [PubMed: 10535958] [Abstract] Cited for: FUNCTION IN TBP IMPORT.
[4]	"Nuclear import of histone H2A and H2B is mediated by a network of karyopherins." Mosammaparast N., Jackson K.R., Guo Y., Brame C.J., Shabanowitz J., Hunt D.F., Pemberton L.F. J. Cell Biol. 153:251-262(2001) [PubMed: 11309407] [Abstract] Cited for: FUNCTION IN HISTONE H2A/H2B IMPORT.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Phosphorylation by casein kinase 2 regulates Nap1 localization and function." Calvert M.E.K., Keck K.M., Ptak C., Shabanowitz J., Hunt D.F., Pemberton L.F. Mol. Cell. Biol. 28:1313-1325(2008) [PubMed: 18086883] [Abstract] Cited for: INTERACTION WITH NAP1, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z49149 Genomic DNA. Translation: CAA89014.1. Z72763 Genomic DNA. Translation: CAA96960.1.
PIR	S53939.
RefSeq	NP_011273.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5881N.
IntAct	P53067. 21 interactions.
STRING	P53067.
Proteomic databases
PeptideAtlas	P53067.
PRIDE	P53067.
Genome annotation databases
Ensembl	YGL241W; YGL241W; YGL241W; Saccharomyces cerevisiae. [Genome view]
GeneID	852610.
KEGG	sce:YGL241W.
NMPDR	fig|4932.3.peg.2376.
Organism-specific databases
CYGD	YGL241w.
SGD	S000003210. KAP114.
Phylogenomic databases
HOGENOM	P53067.
OMA	RSMAKKM
OrthoDB	EOG9X6DRW
Gene expression databases
ArrayExpress	P53067.
Genevestigator	P53067.
GermOnline	YGL241W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR001494. Importin-b_N. [Graphical view]
Gene3D	G3DSA:1.25.10.10. ARM-like. 1 hit.
Pfam	PF03810. IBN_N. 1 hit. [Graphical view]
PROSITE	PS50166. IMPORTIN_B_NT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	971805.

Entry information

Entry name

IMB5_YEAST

Accession

Primary (citable) accession number: P53067

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 24, 2009
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)