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Protein

tRNA-specific adenosine deaminase 1

Gene

TAD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Deaminates adenosine-37 to inosine in tRNA-Ala.

Catalytic activityi

Adenine(37) in tRNA(Ala) + H2O = hypoxanthine(37) in tRNA(Ala) + NH3.1 Publication

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi101 – 1011ZincPROSITE-ProRule annotation
Active sitei103 – 1031Proton donorPROSITE-ProRule annotation
Binding sitei108 – 1081Inositol hexakisphosphateBy similarity
Metal bindingi157 – 1571ZincPROSITE-ProRule annotation
Metal bindingi223 – 2231ZincPROSITE-ProRule annotation
Binding sitei226 – 2261Inositol hexakisphosphateBy similarity
Binding sitei232 – 2321Inositol hexakisphosphateBy similarity
Binding sitei369 – 3691Inositol hexakisphosphateBy similarity
Binding sitei375 – 3751Inositol hexakisphosphateBy similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. RNA binding Source: InterPro
  3. tRNA-specific adenosine-37 deaminase activity Source: SGD

GO - Biological processi

  1. RNA processing Source: InterPro
  2. tRNA modification Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:YGL243W-MONOMER.
BRENDAi3.5.4.34. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-specific adenosine deaminase 1 (EC:3.5.4.34)
Alternative name(s):
tRNA-specific adenosine-37 deaminase
Gene namesi
Name:TAD1
Ordered Locus Names:YGL243W
ORF Names:HRA400
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome VII

Organism-specific databases

CYGDiYGL243w.
SGDiS000003212. TAD1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 400400tRNA-specific adenosine deaminase 1PRO_0000171777Add
BLAST

Proteomic databases

MaxQBiP53065.
PaxDbiP53065.
PRIDEiP53065.

Expressioni

Gene expression databases

GenevestigatoriP53065.

Interactioni

Protein-protein interaction databases

BioGridi32997. 9 interactions.
MINTiMINT-4819462.
STRINGi4932.YGL243W.

Structurei

3D structure databases

ProteinModelPortaliP53065.
SMRiP53065. Positions 56-291.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini76 – 400325A to I editasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ADAT1 family.Curated
Contains 1 A to I editase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG292433.
HOGENOMiHOG000248878.
InParanoidiP53065.
KOiK15440.
OMAiEPPCGDA.
OrthoDBiEOG7NW6K5.

Family and domain databases

InterProiIPR002466. A_deamin.
[Graphical view]
PfamiPF02137. A_deamin. 1 hit.
[Graphical view]
SMARTiSM00552. ADEAMc. 1 hit.
[Graphical view]
PROSITEiPS50141. A_DEAMIN_EDITASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53065-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSCQGTRPC IVNLLTMPSE DKLGEEISTR VINEYSKLKS ACRPIIRPSG
60 70 80 90 100
IREWTILAGV AAINRDGGAN KIEILSIATG VKALPDSELQ RSEGKILHDC
110 120 130 140 150
HAEILALRGA NTVLLNRIQN YNPSSGDKFI QHNDEIPARF NLKENWELAL
160 170 180 190 200
YISRLPCGDA SMSFLNDNCK NDDFIKIEDS DEFQYVDRSV KTILRGRLNF
210 220 230 240 250
NRRNVVRTKP GRYDSNITLS KSCSDKLLMK QRSSVLNCLN YELFEKPVFL
260 270 280 290 300
KYIVIPNLED ETKHHLEQSF HTRLPNLDNE IKFLNCLKPF YDDKLDEEDV
310 320 330 340 350
PGLMCSVKLF MDDFSTEEAI LNGVRNGFYT KSSKPLRKHC QSQVSRFAQW
360 370 380 390 400
ELFKKIRPEY EGISYLEFKS RQKKRSQLII AIKNILSPDG WIPTRTDDVK
Length:400
Mass (Da):45,947
Last modified:September 30, 1996 - v1
Checksum:i2AA9304C549026FF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ007297 Genomic DNA. Translation: CAA07438.1.
Z49149 Genomic DNA. Translation: CAA89012.1.
Z72765 Genomic DNA. Translation: CAA96962.1.
BK006941 Genomic DNA. Translation: DAA07876.1.
PIRiS53937.
RefSeqiNP_011271.1. NM_001181109.1.

Genome annotation databases

EnsemblFungiiYGL243W; YGL243W; YGL243W.
GeneIDi852608.
KEGGisce:YGL243W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ007297 Genomic DNA. Translation: CAA07438.1.
Z49149 Genomic DNA. Translation: CAA89012.1.
Z72765 Genomic DNA. Translation: CAA96962.1.
BK006941 Genomic DNA. Translation: DAA07876.1.
PIRiS53937.
RefSeqiNP_011271.1. NM_001181109.1.

3D structure databases

ProteinModelPortaliP53065.
SMRiP53065. Positions 56-291.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32997. 9 interactions.
MINTiMINT-4819462.
STRINGi4932.YGL243W.

Proteomic databases

MaxQBiP53065.
PaxDbiP53065.
PRIDEiP53065.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL243W; YGL243W; YGL243W.
GeneIDi852608.
KEGGisce:YGL243W.

Organism-specific databases

CYGDiYGL243w.
SGDiS000003212. TAD1.

Phylogenomic databases

eggNOGiNOG292433.
HOGENOMiHOG000248878.
InParanoidiP53065.
KOiK15440.
OMAiEPPCGDA.
OrthoDBiEOG7NW6K5.

Enzyme and pathway databases

BioCyciYEAST:YGL243W-MONOMER.
BRENDAi3.5.4.34. 984.

Miscellaneous databases

NextBioi971799.

Gene expression databases

GenevestigatoriP53065.

Family and domain databases

InterProiIPR002466. A_deamin.
[Graphical view]
PfamiPF02137. A_deamin. 1 hit.
[Graphical view]
SMARTiSM00552. ADEAMc. 1 hit.
[Graphical view]
PROSITEiPS50141. A_DEAMIN_EDITASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Tad1p, a yeast tRNA-specific adenosine deaminase, is related to the mammalian pre-mRNA editing enzymes ADAR1 and ADAR2."
    Gerber A., Grosjean H., Melcher T., Keller W.
    EMBO J. 17:4780-4789(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, CHARACTERIZATION.
    Strain: BMA41.
  2. "The sequence of an 11.1 kb DNA fragment between ADH4 and ADE5 on the left arm of chromosome VII, reveals the presence of eight open reading frames."
    Vandenbol M., Durand P., Portetelle D., Hilger F.
    Yeast 11:1519-1523(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Inositol hexakisphosphate is bound in the ADAR2 core and required for RNA editing."
    Macbeth M.R., Schubert H.L., Vandemark A.P., Lingam A.T., Hill C.P., Bass B.L.
    Science 309:1534-1539(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: COFACTOR.

Entry informationi

Entry nameiTAD1_YEAST
AccessioniPrimary (citable) accession number: P53065
Secondary accession number(s): D6VV92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 30, 1996
Last sequence update: September 30, 1996
Last modified: March 31, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.