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P53065

- TAD1_YEAST

UniProt

P53065 - TAD1_YEAST

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Protein

tRNA-specific adenosine deaminase 1

Gene

TAD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Deaminates adenosine-37 to inosine in tRNA-Ala.

Catalytic activityi

Adenine(37) in tRNA(Ala) + H2O = hypoxanthine(37) in tRNA(Ala) + NH3.1 Publication

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi101 – 1011ZincPROSITE-ProRule annotation
Active sitei103 – 1031Proton donorPROSITE-ProRule annotation
Binding sitei108 – 1081Inositol hexakisphosphateBy similarity
Metal bindingi157 – 1571ZincPROSITE-ProRule annotation
Metal bindingi223 – 2231ZincPROSITE-ProRule annotation
Binding sitei226 – 2261Inositol hexakisphosphateBy similarity
Binding sitei232 – 2321Inositol hexakisphosphateBy similarity
Binding sitei369 – 3691Inositol hexakisphosphateBy similarity
Binding sitei375 – 3751Inositol hexakisphosphateBy similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. RNA binding Source: InterPro
  3. tRNA-specific adenosine-37 deaminase activity Source: SGD

GO - Biological processi

  1. tRNA modification Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:YGL243W-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-specific adenosine deaminase 1 (EC:3.5.4.34)
Alternative name(s):
tRNA-specific adenosine-37 deaminase
Gene namesi
Name:TAD1
Ordered Locus Names:YGL243W
ORF Names:HRA400
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGL243w.
SGDiS000003212. TAD1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 400400tRNA-specific adenosine deaminase 1PRO_0000171777Add
BLAST

Proteomic databases

MaxQBiP53065.
PaxDbiP53065.
PRIDEiP53065.

Expressioni

Gene expression databases

GenevestigatoriP53065.

Interactioni

Protein-protein interaction databases

BioGridi32997. 9 interactions.
MINTiMINT-4819462.
STRINGi4932.YGL243W.

Structurei

3D structure databases

ProteinModelPortaliP53065.
SMRiP53065. Positions 56-291.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini76 – 400325A to I editasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ADAT1 family.Curated
Contains 1 A to I editase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG292433.
HOGENOMiHOG000248878.
InParanoidiP53065.
KOiK15440.
OMAiNDEIPAR.
OrthoDBiEOG7NW6K5.

Family and domain databases

InterProiIPR002466. A_deamin.
[Graphical view]
PfamiPF02137. A_deamin. 1 hit.
[Graphical view]
SMARTiSM00552. ADEAMc. 1 hit.
[Graphical view]
PROSITEiPS50141. A_DEAMIN_EDITASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53065-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVSCQGTRPC IVNLLTMPSE DKLGEEISTR VINEYSKLKS ACRPIIRPSG
60 70 80 90 100
IREWTILAGV AAINRDGGAN KIEILSIATG VKALPDSELQ RSEGKILHDC
110 120 130 140 150
HAEILALRGA NTVLLNRIQN YNPSSGDKFI QHNDEIPARF NLKENWELAL
160 170 180 190 200
YISRLPCGDA SMSFLNDNCK NDDFIKIEDS DEFQYVDRSV KTILRGRLNF
210 220 230 240 250
NRRNVVRTKP GRYDSNITLS KSCSDKLLMK QRSSVLNCLN YELFEKPVFL
260 270 280 290 300
KYIVIPNLED ETKHHLEQSF HTRLPNLDNE IKFLNCLKPF YDDKLDEEDV
310 320 330 340 350
PGLMCSVKLF MDDFSTEEAI LNGVRNGFYT KSSKPLRKHC QSQVSRFAQW
360 370 380 390 400
ELFKKIRPEY EGISYLEFKS RQKKRSQLII AIKNILSPDG WIPTRTDDVK
Length:400
Mass (Da):45,947
Last modified:October 1, 1996 - v1
Checksum:i2AA9304C549026FF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ007297 Genomic DNA. Translation: CAA07438.1.
Z49149 Genomic DNA. Translation: CAA89012.1.
Z72765 Genomic DNA. Translation: CAA96962.1.
BK006941 Genomic DNA. Translation: DAA07876.1.
PIRiS53937.
RefSeqiNP_011271.1. NM_001181109.1.

Genome annotation databases

EnsemblFungiiYGL243W; YGL243W; YGL243W.
GeneIDi852608.
KEGGisce:YGL243W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ007297 Genomic DNA. Translation: CAA07438.1 .
Z49149 Genomic DNA. Translation: CAA89012.1 .
Z72765 Genomic DNA. Translation: CAA96962.1 .
BK006941 Genomic DNA. Translation: DAA07876.1 .
PIRi S53937.
RefSeqi NP_011271.1. NM_001181109.1.

3D structure databases

ProteinModelPortali P53065.
SMRi P53065. Positions 56-291.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32997. 9 interactions.
MINTi MINT-4819462.
STRINGi 4932.YGL243W.

Proteomic databases

MaxQBi P53065.
PaxDbi P53065.
PRIDEi P53065.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGL243W ; YGL243W ; YGL243W .
GeneIDi 852608.
KEGGi sce:YGL243W.

Organism-specific databases

CYGDi YGL243w.
SGDi S000003212. TAD1.

Phylogenomic databases

eggNOGi NOG292433.
HOGENOMi HOG000248878.
InParanoidi P53065.
KOi K15440.
OMAi NDEIPAR.
OrthoDBi EOG7NW6K5.

Enzyme and pathway databases

BioCyci YEAST:YGL243W-MONOMER.

Miscellaneous databases

NextBioi 971799.

Gene expression databases

Genevestigatori P53065.

Family and domain databases

InterProi IPR002466. A_deamin.
[Graphical view ]
Pfami PF02137. A_deamin. 1 hit.
[Graphical view ]
SMARTi SM00552. ADEAMc. 1 hit.
[Graphical view ]
PROSITEi PS50141. A_DEAMIN_EDITASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Tad1p, a yeast tRNA-specific adenosine deaminase, is related to the mammalian pre-mRNA editing enzymes ADAR1 and ADAR2."
    Gerber A., Grosjean H., Melcher T., Keller W.
    EMBO J. 17:4780-4789(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, CHARACTERIZATION.
    Strain: BMA41.
  2. "The sequence of an 11.1 kb DNA fragment between ADH4 and ADE5 on the left arm of chromosome VII, reveals the presence of eight open reading frames."
    Vandenbol M., Durand P., Portetelle D., Hilger F.
    Yeast 11:1519-1523(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Inositol hexakisphosphate is bound in the ADAR2 core and required for RNA editing."
    Macbeth M.R., Schubert H.L., Vandemark A.P., Lingam A.T., Hill C.P., Bass B.L.
    Science 309:1534-1539(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: COFACTOR.

Entry informationi

Entry nameiTAD1_YEAST
AccessioniPrimary (citable) accession number: P53065
Secondary accession number(s): D6VV92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 26, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3