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P53065

- TAD1_YEAST

UniProt

P53065 - TAD1_YEAST

Protein

tRNA-specific adenosine deaminase 1

Gene

TAD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Deaminates adenosine-37 to inosine in tRNA-Ala.

    Catalytic activityi

    Adenine(37) in tRNA(Ala) + H2O = hypoxanthine(37) in tRNA(Ala) + NH3.1 Publication

    Cofactori

    Binds 1 inositol hexakisphosphate (IP6) per subunit.1 Publication
    Zinc.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi101 – 1011ZincPROSITE-ProRule annotation
    Active sitei103 – 1031Proton donorPROSITE-ProRule annotation
    Binding sitei108 – 1081Inositol hexakisphosphateBy similarity
    Metal bindingi157 – 1571ZincPROSITE-ProRule annotation
    Metal bindingi223 – 2231ZincPROSITE-ProRule annotation
    Binding sitei226 – 2261Inositol hexakisphosphateBy similarity
    Binding sitei232 – 2321Inositol hexakisphosphateBy similarity
    Binding sitei369 – 3691Inositol hexakisphosphateBy similarity
    Binding sitei375 – 3751Inositol hexakisphosphateBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. RNA binding Source: InterPro
    3. tRNA-specific adenosine-37 deaminase activity Source: SGD

    GO - Biological processi

    1. tRNA modification Source: SGD

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:YGL243W-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-specific adenosine deaminase 1 (EC:3.5.4.34)
    Alternative name(s):
    tRNA-specific adenosine-37 deaminase
    Gene namesi
    Name:TAD1
    Ordered Locus Names:YGL243W
    ORF Names:HRA400
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGL243w.
    SGDiS000003212. TAD1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 400400tRNA-specific adenosine deaminase 1PRO_0000171777Add
    BLAST

    Proteomic databases

    MaxQBiP53065.
    PaxDbiP53065.
    PRIDEiP53065.

    Expressioni

    Gene expression databases

    GenevestigatoriP53065.

    Interactioni

    Protein-protein interaction databases

    BioGridi32997. 9 interactions.
    MINTiMINT-4819462.
    STRINGi4932.YGL243W.

    Structurei

    3D structure databases

    ProteinModelPortaliP53065.
    SMRiP53065. Positions 56-291.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini76 – 400325A to I editasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ADAT1 family.Curated
    Contains 1 A to I editase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG292433.
    HOGENOMiHOG000248878.
    KOiK15440.
    OMAiNDEIPAR.
    OrthoDBiEOG7NW6K5.

    Family and domain databases

    InterProiIPR002466. A_deamin.
    [Graphical view]
    PfamiPF02137. A_deamin. 1 hit.
    [Graphical view]
    SMARTiSM00552. ADEAMc. 1 hit.
    [Graphical view]
    PROSITEiPS50141. A_DEAMIN_EDITASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53065-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVSCQGTRPC IVNLLTMPSE DKLGEEISTR VINEYSKLKS ACRPIIRPSG    50
    IREWTILAGV AAINRDGGAN KIEILSIATG VKALPDSELQ RSEGKILHDC 100
    HAEILALRGA NTVLLNRIQN YNPSSGDKFI QHNDEIPARF NLKENWELAL 150
    YISRLPCGDA SMSFLNDNCK NDDFIKIEDS DEFQYVDRSV KTILRGRLNF 200
    NRRNVVRTKP GRYDSNITLS KSCSDKLLMK QRSSVLNCLN YELFEKPVFL 250
    KYIVIPNLED ETKHHLEQSF HTRLPNLDNE IKFLNCLKPF YDDKLDEEDV 300
    PGLMCSVKLF MDDFSTEEAI LNGVRNGFYT KSSKPLRKHC QSQVSRFAQW 350
    ELFKKIRPEY EGISYLEFKS RQKKRSQLII AIKNILSPDG WIPTRTDDVK 400
    Length:400
    Mass (Da):45,947
    Last modified:October 1, 1996 - v1
    Checksum:i2AA9304C549026FF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ007297 Genomic DNA. Translation: CAA07438.1.
    Z49149 Genomic DNA. Translation: CAA89012.1.
    Z72765 Genomic DNA. Translation: CAA96962.1.
    BK006941 Genomic DNA. Translation: DAA07876.1.
    PIRiS53937.
    RefSeqiNP_011271.1. NM_001181109.1.

    Genome annotation databases

    EnsemblFungiiYGL243W; YGL243W; YGL243W.
    GeneIDi852608.
    KEGGisce:YGL243W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ007297 Genomic DNA. Translation: CAA07438.1 .
    Z49149 Genomic DNA. Translation: CAA89012.1 .
    Z72765 Genomic DNA. Translation: CAA96962.1 .
    BK006941 Genomic DNA. Translation: DAA07876.1 .
    PIRi S53937.
    RefSeqi NP_011271.1. NM_001181109.1.

    3D structure databases

    ProteinModelPortali P53065.
    SMRi P53065. Positions 56-291.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32997. 9 interactions.
    MINTi MINT-4819462.
    STRINGi 4932.YGL243W.

    Proteomic databases

    MaxQBi P53065.
    PaxDbi P53065.
    PRIDEi P53065.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL243W ; YGL243W ; YGL243W .
    GeneIDi 852608.
    KEGGi sce:YGL243W.

    Organism-specific databases

    CYGDi YGL243w.
    SGDi S000003212. TAD1.

    Phylogenomic databases

    eggNOGi NOG292433.
    HOGENOMi HOG000248878.
    KOi K15440.
    OMAi NDEIPAR.
    OrthoDBi EOG7NW6K5.

    Enzyme and pathway databases

    BioCyci YEAST:YGL243W-MONOMER.

    Miscellaneous databases

    NextBioi 971799.

    Gene expression databases

    Genevestigatori P53065.

    Family and domain databases

    InterProi IPR002466. A_deamin.
    [Graphical view ]
    Pfami PF02137. A_deamin. 1 hit.
    [Graphical view ]
    SMARTi SM00552. ADEAMc. 1 hit.
    [Graphical view ]
    PROSITEi PS50141. A_DEAMIN_EDITASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Tad1p, a yeast tRNA-specific adenosine deaminase, is related to the mammalian pre-mRNA editing enzymes ADAR1 and ADAR2."
      Gerber A., Grosjean H., Melcher T., Keller W.
      EMBO J. 17:4780-4789(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, CHARACTERIZATION.
      Strain: BMA41.
    2. "The sequence of an 11.1 kb DNA fragment between ADH4 and ADE5 on the left arm of chromosome VII, reveals the presence of eight open reading frames."
      Vandenbol M., Durand P., Portetelle D., Hilger F.
      Yeast 11:1519-1523(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Inositol hexakisphosphate is bound in the ADAR2 core and required for RNA editing."
      Macbeth M.R., Schubert H.L., Vandemark A.P., Lingam A.T., Hill C.P., Bass B.L.
      Science 309:1534-1539(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: COFACTOR.

    Entry informationi

    Entry nameiTAD1_YEAST
    AccessioniPrimary (citable) accession number: P53065
    Secondary accession number(s): D6VV92
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3