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P53065 (TAD1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA-specific adenosine deaminase 1

EC=3.5.4.34
Alternative name(s):
tRNA-specific adenosine-37 deaminase
Gene names
Name:TAD1
Ordered Locus Names:YGL243W
ORF Names:HRA400
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length400 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Deaminates adenosine-37 to inosine in tRNA-Ala.

Catalytic activity

Adenine37 in tRNA(Ala) + H2O = hypoxanthine37 in tRNA(Ala) + NH3. Ref.1

Cofactor

Binds 1 inositol hexakisphosphate (IP6) per subunit. Ref.5

Zinc By similarity. Ref.5

Sequence similarities

Belongs to the ADAT1 family.

Contains 1 A to I editase domain.

Ontologies

Keywords
   Biological processtRNA processing
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtRNA modification

Inferred from direct assay Ref.1. Source: SGD

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: InterPro

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

tRNA-specific adenosine-37 deaminase activity

Inferred from direct assay Ref.1. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 400400tRNA-specific adenosine deaminase 1
PRO_0000171777

Regions

Domain76 – 400325A to I editase

Sites

Active site1031Proton donor By similarity
Metal binding1011Zinc By similarity
Metal binding1571Zinc By similarity
Metal binding2231Zinc By similarity
Binding site1081Inositol hexakisphosphate By similarity
Binding site2261Inositol hexakisphosphate By similarity
Binding site2321Inositol hexakisphosphate By similarity
Binding site3691Inositol hexakisphosphate By similarity
Binding site3751Inositol hexakisphosphate By similarity

Sequences

Sequence LengthMass (Da)Tools
P53065 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 2AA9304C549026FF

FASTA40045,947
        10         20         30         40         50         60 
MVSCQGTRPC IVNLLTMPSE DKLGEEISTR VINEYSKLKS ACRPIIRPSG IREWTILAGV 

        70         80         90        100        110        120 
AAINRDGGAN KIEILSIATG VKALPDSELQ RSEGKILHDC HAEILALRGA NTVLLNRIQN 

       130        140        150        160        170        180 
YNPSSGDKFI QHNDEIPARF NLKENWELAL YISRLPCGDA SMSFLNDNCK NDDFIKIEDS 

       190        200        210        220        230        240 
DEFQYVDRSV KTILRGRLNF NRRNVVRTKP GRYDSNITLS KSCSDKLLMK QRSSVLNCLN 

       250        260        270        280        290        300 
YELFEKPVFL KYIVIPNLED ETKHHLEQSF HTRLPNLDNE IKFLNCLKPF YDDKLDEEDV 

       310        320        330        340        350        360 
PGLMCSVKLF MDDFSTEEAI LNGVRNGFYT KSSKPLRKHC QSQVSRFAQW ELFKKIRPEY 

       370        380        390        400 
EGISYLEFKS RQKKRSQLII AIKNILSPDG WIPTRTDDVK 

« Hide

References

« Hide 'large scale' references
[1]"Tad1p, a yeast tRNA-specific adenosine deaminase, is related to the mammalian pre-mRNA editing enzymes ADAR1 and ADAR2."
Gerber A., Grosjean H., Melcher T., Keller W.
EMBO J. 17:4780-4789(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, CHARACTERIZATION.
Strain: BMA41.
[2]"The sequence of an 11.1 kb DNA fragment between ADH4 and ADE5 on the left arm of chromosome VII, reveals the presence of eight open reading frames."
Vandenbol M., Durand P., Portetelle D., Hilger F.
Yeast 11:1519-1523(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Inositol hexakisphosphate is bound in the ADAR2 core and required for RNA editing."
Macbeth M.R., Schubert H.L., Vandemark A.P., Lingam A.T., Hill C.P., Bass B.L.
Science 309:1534-1539(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: COFACTOR.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ007297 Genomic DNA. Translation: CAA07438.1.
Z49149 Genomic DNA. Translation: CAA89012.1.
Z72765 Genomic DNA. Translation: CAA96962.1.
BK006941 Genomic DNA. Translation: DAA07876.1.
PIRS53937.
RefSeqNP_011271.1. NM_001181109.1.

3D structure databases

ProteinModelPortalP53065.
SMRP53065. Positions 56-291.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32997. 9 interactions.
MINTMINT-4819462.
STRING4932.YGL243W.

Proteomic databases

PaxDbP53065.
PRIDEP53065.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGL243W; YGL243W; YGL243W.
GeneID852608.
KEGGsce:YGL243W.

Organism-specific databases

CYGDYGL243w.
SGDS000003212. TAD1.

Phylogenomic databases

eggNOGNOG292433.
HOGENOMHOG000248878.
KOK15440.
OMAEPPCGDA.
OrthoDBEOG7NW6K5.

Enzyme and pathway databases

BioCycYEAST:YGL243W-MONOMER.

Gene expression databases

GenevestigatorP53065.

Family and domain databases

InterProIPR002466. A_deamin.
[Graphical view]
PfamPF02137. A_deamin. 1 hit.
[Graphical view]
SMARTSM00552. ADEAMc. 1 hit.
[Graphical view]
PROSITEPS50141. A_DEAMIN_EDITASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio971799.

Entry information

Entry nameTAD1_YEAST
AccessionPrimary (citable) accession number: P53065
Secondary accession number(s): D6VV92
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: February 19, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families