Reviewed,
UniProtKB/Swiss-Prot P53064 (RTF1_YEAST)
Last modified
December 15, 2009.
Version 80.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: RNA polymerase-associated protein RTF1 | ||||||||
| Gene names |
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| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 558 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | The PAF1 complex is a multifunctional complex. Involved in transcription initiation via genetic interactions with TATA-binding proteins. Involved in elongation. It regulates 3'-end formation of snR47 by modulating the recruitment or stable association of NRD1 and NAB3 with RNA polymerase II. Also has a role in transcription-coupled histone modification. Required for activation of RAD6 ubiquitin conjugate and the BRE1 ubiquitin ligase which ubiquitylate 'Lys-126' histone H2B. Activates the SET1 histone methyltransferase complex for methylation of 'Lys-4' of histone H3 and for methylation of 'Lys-73' of histone H3 by DOT1 and 'Lys-36' of histone H3 by SET2. Important for TATA site selection by TBP. Directly or indirectly regulates the DNA-binding properties of SPT15, the TATA box-binding protein, and the relative activities of different TATA elements. Ref.7 Ref.8 |
| Subunit structure | Component of the PAF1 complex which consists of at least CDC73, CTR9, LEO1, PAF1 and RTF1. |
| Subcellular location | |
| Miscellaneous | Present with 6510 molecules/cell in log phase SD medium. Ref.6 |
| Sequence similarities | Contains 1 Plus3 domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Transcription Transcription regulation |
| Cellular component | Nucleus |
| Molecular function | Activator |
| PTM | Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | RNA elongation from RNA polymerase II promoter Inferred from physical interaction. Source: SGD histone methylationInferred from mutant phenotype. Source: SGD histone monoubiquitinationInferred from mutant phenotype. Source: SGD negative regulation of transposition, RNA-mediatedInferred from mutant phenotype. Source: SGD regulation of transcriptionInferred from electronic annotation. Source: UniProtKB-KW transcription initiationInferred from electronic annotation. Source: InterPro |
| Cellular component | Cdc73/Paf1 complex Ref.4 Inferred from physical interaction. Source: SGD |
| Molecular function | DNA binding Inferred from electronic annotation. Source: InterPro RNA polymerase II transcription elongation factor activityInferred from physical interaction. Source: SGD |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 558 | 558 | RNA polymerase-associated protein RTF1 | PRO_0000097512 | |||||
Regions | |||||||||
| Domain | 238 – 370 | 133 | Plus3 | ||||||
| Compositional bias | 72 – 76 | 5 | Poly-Glu | ||||||
Amino acid modifications | |||||||||
| Modified residue | 17 | 1 | Phosphoserine Ref.9 | ||||||
| Modified residue | 69 | 1 | Phosphotyrosine Ref.9 | ||||||
| Modified residue | 71 | 1 | Phosphoserine Ref.9 | ||||||
| Modified residue | 477 | 1 | Phosphoserine Ref.9 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Identification of RTF1, a novel gene important for TATA site selection by TATA box-binding protein in Saccharomyces cerevisiae." Stolinski L.A., Eisenmann D.M., Arndt K.M. Mol. Cell. Biol. 17:4490-4500(1997) [PubMed: 9234706] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "The sequence of an 11.1 kb DNA fragment between ADH4 and ADE5 on the left arm of chromosome VII, reveals the presence of eight open reading frames." Vandenbol M., Durand P., Portetelle D., Hilger F. Yeast 11:1519-1523(1995) [PubMed: 8750240] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [3] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K.Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [4] | "Ctr9, Rtf1, and Leo1 are components of the Paf1/RNA polymerase II complex." Mueller C.L., Jaehning J.A. Mol. Cell. Biol. 22:1971-1980(2002) [PubMed: 11884586] [Abstract] Cited for: IDENTIFICATION IN THE PAF1 COMPLEX. |
| [5] | "Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. |
| [6] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [7] | "Separation of the Saccharomyces cerevisiae Paf1 complex from RNA polymerase II results in changes in its subnuclear localization." Porter S.E., Penheiter K.L., Jaehning J.A. Eukaryot. Cell 4:209-220(2005) [PubMed: 15643076] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION. |
| [8] | "A requirement for the Saccharomyces cerevisiae Paf1 complex in snoRNA 3' end formation." Sheldon K.E., Mauger D.M., Arndt K.M. Mol. Cell 20:225-236(2005) [PubMed: 16246725] [Abstract] Cited for: FUNCTION. |
| [9] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17; TYR-69; SER-71 AND SER-477, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| U86702 Genomic DNA. Translation: AAB47535.1. Z49149 Genomic DNA. Translation: CAA89011.1. Different initiation. Z72766 Genomic DNA. Translation: CAA96963.1. Z72767 Genomic DNA. Translation: CAA96965.1. | |
| PIR | S53936. |
| RefSeq | NP_011270.1. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-4065N. |
| IntAct | P53064. 16 interactions. |
| STRING | P53064. |
Proteomic databases | |
| PeptideAtlas | P53064. |
| PRIDE | P53064. |
Genome annotation databases | |
| Ensembl | YGL244W; YGL244W; YGL244W; Saccharomyces cerevisiae. [Genome view] |
| GeneID | 852607. |
| KEGG | sce:YGL244W. |
| NMPDR | fig|4932.3.peg.2373. |
Organism-specific databases | |
| CYGD | YGL244w. |
| SGD | S000003213. RTF1. |
Phylogenomic databases | |
| HOGENOM | HBG397816. |
| OMA | NASEMKP. |
| OrthoDB | EOG9V9W70. |
Gene expression databases | |
| ArrayExpress | P53064. |
| Genevestigator | P53064. |
| GermOnline | YGL244W. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR004343. Plus-3. IPR018144. Plus3-dom_subgr. [Graphical view] |
| Pfam | PF03126. Plus-3. 1 hit. [Graphical view] |
| SMART | SM00719. Plus3. 1 hit. [Graphical view] |
| PROSITE | PS51360. PLUS3. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 971796. |
Entry information
| Entry name | RTF1_YEAST | ||||||||
| Accession | Primary (citable) accession number: P53064 Secondary accession number(s): P89115 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome VII Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names |

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