P53059 (MNT2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-1,3-mannosyltransferase MNT2
EC=2.4.1.-

Gene names

Name:	MNT2
Ordered Locus Names:	YGL257C
ORF Names:	NRD558

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	558 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mannosyltransferase involved in adding the 4th and 5th mannose residues of O-linked glycans.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Potential.
Miscellaneous	Present with 1170 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the MNN1/MNT family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein O-linked glycosylation Inferred from direct assay Ref.4. Source: SGD
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-1,3-mannosyltransferase activity Inferred from direct assay Ref.4. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
THI80	P35202	1	EBI-2092438,EBI-19195

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 558	558	Alpha-1,3-mannosyltransferase MNT2		PRO_0000080559
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 27	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	28 – 558	531	Lumenal Potential
Amino acid modifications
	Glycosylation	187	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P53059 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 3E58ED62B4E291B6	FASTA	558	64,853
	10 20 30 40 50 60 MRRKNRLFIL VVLLGIVLVV YYSQLNSLDL VEPVQSSSSG NGGCWSYYEG LTPGWLNDFY 70 80 90 100 110 120 DVNQITPNPA KDVIELVTRI KIFFNCLQQV DGHNIQRLRD IEKKLFPYIN FEKLETDESA 130 140 150 160 170 180 FWHTTTRWNG EVYHASMLEF DPKNHQFLRS KPINFDTGLS FWENWLHTVT QSGSKGIVIS 190 200 210 220 230 240 ASDVQLNETI RLLKVLRFIK NDYPIQIVHN ADLSQDSMKS IIKYARSLDT AEYPAQELWF 250 260 270 280 290 300 LNVHSLLNPK YSKKFTTYSN KWLALTFSSF EIPILMDSDT VPFVSIEKFY ELEEFQKTGV 310 320 330 340 350 360 LFFKDRVISD DLFESSELKI LREIVYGCIG LDLEDESKIH EQVEDPVVAQ VLENMFIKKY 370 380 390 400 410 420 KHHLESGLVI LHKGKHLFSM LTSIALQFSP IAEYFHGDKD FFWLGELLSN NRFTFHPVDA 430 440 450 460 470 480 SNIGQLGNVV SKESTGEFYQ ICSVQLSHTD RDGSLLWLNG GLNICKKTSW EYDYEHRQRL 490 500 510 520 530 540 NDMFQNADEL REYYASPVKL EGIIIPDTSI SGWINSGECF LFNYCTLFKE GEFGKLIKFK 550 EDEKLRLSQI VDIWNKDI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of a 39,411 bp DNA fragment covering the left end of chromosome VII of Saccharomyces cerevisiae." Coissac E., Maillier E., Robineau S., Netter P. Yeast 12:1555-1562(1996) [PubMed: 8972578] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Mnt2p and Mnt3p of Saccharomyces cerevisiae are members of the Mnn1p family of alpha-1,3-mannosyltransferases responsible for adding the terminal mannose residues of O-linked oligosaccharides." Romero P.A., Lussier M., Veronneau S., Sdicu A.-M., Herscovics A., Bussey H. Glycobiology 9:1045-1051(1999) [PubMed: 10521541] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X94357 Genomic DNA. Translation: CAA64130.1. Z72779 Genomic DNA. Translation: CAA96977.1. BK006941 Genomic DNA. Translation: DAA07862.1.
PIR	S61604.
RefSeq	NP_011257.1. NM_001181123.1.
3D structure databases
ProteinModelPortal	P53059.
ModBase	Search...
Protein-protein interaction databases
IntAct	P53059. 1 interaction.
STRING	P53059.
Protein family/group databases
CAZy	GT71. Glycosyltransferase Family 71.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL257C; YGL257C; YGL257C.
GeneID	852635.
KEGG	sce:YGL257C.
NMPDR	fig|4932.3.peg.2360.
Organism-specific databases
CYGD	YGL257c.
SGD	S000003226. MNT2.
Phylogenomic databases
eggNOG	fuNOG10625.
GeneTree	EFGT00050000003823.
HOGENOM	HBG204460.
OrthoDB	EOG4PVS70.
Gene expression databases
ArrayExpress	P53059.
Genevestigator	P53059.
GermOnline	YGL257C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR022751. Alpha_mannosyltransferase. [Graphical view]
KO	K10968.
Pfam	PF11051. Mannosyl_trans3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971876.

Entry information

Entry name

MNT2_YEAST

Accession

Primary (citable) accession number: P53059
Secondary accession number(s): D6VV78

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program