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Protein

Alpha-1,3-mannosyltransferase MNT2

Gene

MNT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Mannosyltransferase involved in adding the 4th and 5th mannose residues of O-linked glycans.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • alpha-1,3-mannosyltransferase activity Source: SGD

GO - Biological processi

  • protein O-linked glycosylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciYEAST:G3O-30725-MONOMER.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT71. Glycosyltransferase Family 71.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,3-mannosyltransferase MNT2 (EC:2.4.1.-)
Gene namesi
Name:MNT2
Ordered Locus Names:YGL257C
ORF Names:NRD558
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL257C.
SGDiS000003226. MNT2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence analysis
Transmembranei7 – 2721Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini28 – 558531LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

  • Golgi apparatus Source: SGD
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 558558Alpha-1,3-mannosyltransferase MNT2PRO_0000080559Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi187 – 1871N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP53059.

Interactioni

Protein-protein interaction databases

BioGridi33022. 17 interactions.

Structurei

3D structure databases

ProteinModelPortaliP53059.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MNN1/MNT family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000003622.
HOGENOMiHOG000113620.
InParanoidiP53059.
KOiK10968.
OMAiWINSGEC.
OrthoDBiEOG092C553O.

Family and domain databases

Gene3Di3.90.550.10. 2 hits.
InterProiIPR022751. Alpha_mannosyltransferase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF11051. Mannosyl_trans3. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 2 hits.

Sequencei

Sequence statusi: Complete.

P53059-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRKNRLFIL VVLLGIVLVV YYSQLNSLDL VEPVQSSSSG NGGCWSYYEG
60 70 80 90 100
LTPGWLNDFY DVNQITPNPA KDVIELVTRI KIFFNCLQQV DGHNIQRLRD
110 120 130 140 150
IEKKLFPYIN FEKLETDESA FWHTTTRWNG EVYHASMLEF DPKNHQFLRS
160 170 180 190 200
KPINFDTGLS FWENWLHTVT QSGSKGIVIS ASDVQLNETI RLLKVLRFIK
210 220 230 240 250
NDYPIQIVHN ADLSQDSMKS IIKYARSLDT AEYPAQELWF LNVHSLLNPK
260 270 280 290 300
YSKKFTTYSN KWLALTFSSF EIPILMDSDT VPFVSIEKFY ELEEFQKTGV
310 320 330 340 350
LFFKDRVISD DLFESSELKI LREIVYGCIG LDLEDESKIH EQVEDPVVAQ
360 370 380 390 400
VLENMFIKKY KHHLESGLVI LHKGKHLFSM LTSIALQFSP IAEYFHGDKD
410 420 430 440 450
FFWLGELLSN NRFTFHPVDA SNIGQLGNVV SKESTGEFYQ ICSVQLSHTD
460 470 480 490 500
RDGSLLWLNG GLNICKKTSW EYDYEHRQRL NDMFQNADEL REYYASPVKL
510 520 530 540 550
EGIIIPDTSI SGWINSGECF LFNYCTLFKE GEFGKLIKFK EDEKLRLSQI

VDIWNKDI
Length:558
Mass (Da):64,853
Last modified:October 1, 1996 - v1
Checksum:i3E58ED62B4E291B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94357 Genomic DNA. Translation: CAA64130.1.
Z72779 Genomic DNA. Translation: CAA96977.1.
BK006941 Genomic DNA. Translation: DAA07862.1.
PIRiS61604.
RefSeqiNP_011257.1. NM_001181123.1.

Genome annotation databases

EnsemblFungiiYGL257C; YGL257C; YGL257C.
GeneIDi852635.
KEGGisce:YGL257C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94357 Genomic DNA. Translation: CAA64130.1.
Z72779 Genomic DNA. Translation: CAA96977.1.
BK006941 Genomic DNA. Translation: DAA07862.1.
PIRiS61604.
RefSeqiNP_011257.1. NM_001181123.1.

3D structure databases

ProteinModelPortaliP53059.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33022. 17 interactions.

Protein family/group databases

CAZyiGT71. Glycosyltransferase Family 71.

Proteomic databases

MaxQBiP53059.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL257C; YGL257C; YGL257C.
GeneIDi852635.
KEGGisce:YGL257C.

Organism-specific databases

EuPathDBiFungiDB:YGL257C.
SGDiS000003226. MNT2.

Phylogenomic databases

GeneTreeiENSGT00390000003622.
HOGENOMiHOG000113620.
InParanoidiP53059.
KOiK10968.
OMAiWINSGEC.
OrthoDBiEOG092C553O.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciYEAST:G3O-30725-MONOMER.

Miscellaneous databases

PROiP53059.

Family and domain databases

Gene3Di3.90.550.10. 2 hits.
InterProiIPR022751. Alpha_mannosyltransferase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF11051. Mannosyl_trans3. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiMNT2_YEAST
AccessioniPrimary (citable) accession number: P53059
Secondary accession number(s): D6VV78
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1170 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.