ID   MALX3_YEAST             Reviewed;         589 AA.
AC   P53051;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   16-JUN-2009, entry version 65.
DE   RecName: Full=Probable alpha-glucosidase FSP2;
DE            EC=3.2.1.20;
DE   AltName: Full=Maltase;
DE   AltName: Full=Flocculent-specific protein 2;
GN   Name=FSP2; OrderedLocusNames=YGR287C;
OS   Saccharomyces cerevisiae (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=4932;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Tonouchi A.;
RL   Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 96604 / S288c / FY1679;
RX   MEDLINE=97245295; PubMed=9090054;
RX   DOI=10.1002/(SICI)1097-0061(19970315)13:3<251::AID-YEA63>3.3.CO;2-I;
RA   Volckaert G., Voet M., Robben J.;
RT   "Sequence analysis of a near-subtelomeric 35.4 kb DNA segment on the
RT   right arm of chromosome VII from Saccharomyces cerevisiae carrying the
RT   MAL1 locus reveals 15 complete open reading frames, including ZUO1,
RT   BGL2 and BIO2 genes and an ABC transporter gene.";
RL   Yeast 13:251-259(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 96604 / S288c / FY1679;
RX   MEDLINE=97313265; PubMed=9169869;
RA   Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M.,
RA   Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J.,
RA   Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E.,
RA   Clemente M.L., Coblenz A., Coglievina M., Coissac E., Defoor E.,
RA   Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B.,
RA   Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L.,
RA   Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M.,
RA   Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M.,
RA   Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B.,
RA   Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W.,
RA   Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A.,
RA   Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA   Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S.,
RA   Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L.,
RA   Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S.,
RA   Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J.,
RA   Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M.,
RA   Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B.,
RA   Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J.,
RA   Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M.,
RA   van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M.,
RA   Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H.,
RA   Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M.,
RA   Zollner A., Kleine K.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL   Nature 387:81-84(1997).
RN   [4]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A.,
RA   Dephoure N., O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
CC   -!- FUNCTION: Probable alpha-glucosidase involved in maltose
CC       metabolism.
CC   -!- CATALYTIC ACTIVITY: Hydrolysis of terminal, non-reducing (1->4)-
CC       linked alpha-D-glucose residues with release of alpha-D-glucose.
CC   -!- INTERACTION:
CC       P22696:ESS1; NbExp=1; IntAct=EBI-10464, EBI-6679;
CC       P33203:PRP40; NbExp=1; IntAct=EBI-10464, EBI-701;
CC       P39940:RSP5; NbExp=1; IntAct=EBI-10464, EBI-16219;
CC       P40318:SSM4; NbExp=1; IntAct=EBI-10464, EBI-18208;
CC       Q06525:URN1; NbExp=1; IntAct=EBI-10464, EBI-35138;
CC   -!- MISCELLANEOUS: Present with 752 molecules/cell in log phase SD
CC       medium.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
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DR   EMBL; D43761; BAA07818.1; -; Genomic_DNA.
DR   EMBL; Z73072; CAA97319.1; -; Genomic_DNA.
DR   EMBL; Z73073; CAA97321.1; -; Genomic_DNA.
DR   PIR; S59370; S59370.
DR   RefSeq; NP_011803.1; -.
DR   HSSP; P21332; 1UOK.
DR   IntAct; P53051; 5.
DR   CAZy; GH13; Glycoside Hydrolase Family 13.
DR   Ensembl; YGR287C; Saccharomyces cerevisiae.
DR   GeneID; 853204; -.
DR   GenomeReviews; Y13135_GR; YGR287C.
DR   KEGG; sce:YGR287C; -.
DR   NMPDR; fig|4932.3.peg.2934; -.
DR   CYGD; YGR287c; -.
DR   SGD; S000003519; YGR287C.
DR   HOGENOM; P53051; -.
DR   OMA; P53051; WENEVTR.
DR   BRENDA; 3.2.1.20; 250.
DR   NextBio; 973383; -.
DR   ArrayExpress; P53051; -.
DR   GermOnline; YGR287C; Saccharomyces cerevisiae.
DR   GO; GO:0005739; C:mitochondrion; IDA:SGD.
DR   GO; GO:0004558; F:alpha-glucosidase activity; IEA:EC.
DR   GO; GO:0043169; F:cation binding; IEA:InterPro.
DR   GO; GO:0004574; F:oligo-1,6-glucosidase activity; IDA:SGD.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   GO; GO:0000023; P:maltose metabolic process; IC:SGD.
DR   InterPro; IPR013780; Glyco_hydro_13_b.
DR   InterPro; IPR006047; Glyco_hydro_13_cat.
DR   InterPro; IPR006589; Glyco_hydro_13_sub_cat.
DR   InterPro; IPR013781; Glyco_hydro_sg_catalytic.
DR   Gene3D; G3DSA:2.60.40.1180; Glyco_hydro_13_b; 1.
DR   Gene3D; G3DSA:3.20.20.80; Glyco_hydro_cat; 1.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   SMART; SM00642; Aamy; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Glycosidase; Hydrolase; Maltose metabolism.
FT   CHAIN         1    589       Probable alpha-glucosidase FSP2.
FT                                /FTId=PRO_0000054333.
FT   ACT_SITE    215    215       Nucleophile (By similarity).
FT   ACT_SITE    277    277       Proton donor (By similarity).
FT   ACT_SITE    352    352       By similarity.
SQ   SEQUENCE   589 AA;  68592 MW;  08869180588053B6 CRC64;
     MTISSAHPET EPKWWKEATF YQIYPASFKD SNDDGWGDMK GIASKLEYIK ELGADAIWIS
     PFYDSPQDDM GYDIANYEKV WPTYGTNEDC FALIEKTHKL GMKFITDLVI NHCSSEHEWF
     KESRSSKTNP KRDWFFWRPP KGYDAEGKPI PPNNWKSYFG GSAWTFDEKT QEFYLRLFCS
     TQPDLNWENE DCRKAIYESA VGYWLDHGVD GFRIDVGSLY SKVVGLPDAP VVDKNSTWQS
     SDPYTLNGPR IHEFHQEMNQ FIRNRVKDGR EIMTVGEMQH ASDETKRLYT SASRHELSEL
     FNFSHTDVGT SPLFRYNLVP FELKDWKIAL AELFRYINGT DCWSTIYLEN HDQPRSITRF
     GDDSPKNRVI SGKLLSVLLS ALTGTLYVYQ GQELGQINFK NWPVEKYEDV EIRNNYNAIK
     EEHGENSEEM KKFLEAIALI SRDHARTPMQ WSREEPNAGF SGPSAKPWFY LNDSFREGIN
     VEDEIKDPNS VLNFWKEALK FRKAHKDITV YGYDFEFIDL DNKKLFSFTK KYNNKTLFAA
     LNFSSDATDF KIPNDDSSFK LEFGNYPKKE VDASSRTLKP WEGRIYISE
//