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Protein

Oligo-1,6-glucosidase IMA1

Gene

IMA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Major isomaltase (alpha-1,6-glucosidase) required for isomaltose utilization. Preferentially hydrolyzes isomaltose, palatinose, and methyl-alpha-glucoside, with little activity towards isomaltotriose or longer oligosaccharides. Does not hydrolyze maltose.2 Publications

Catalytic activityi

Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei215 – 2151Nucleophile
Active sitei277 – 2771Proton donor
Sitei352 – 3521Transition state stabilizer

GO - Molecular functioni

  • oligo-1,6-glucosidase activity Source: SGD
  • sucrose alpha-glucosidase activity Source: SGD

GO - Biological processi

  • disaccharide catabolic process Source: SGD
  • maltose metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Maltose metabolism

Enzyme and pathway databases

BioCyciYEAST:YGR287C-MONOMER.
BRENDAi3.2.1.10. 984.
ReactomeiR-SCE-352230. Amino acid transport across the plasma membrane.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.
mycoCLAPiOGL13C_YEAST.

Names & Taxonomyi

Protein namesi
Recommended name:
Oligo-1,6-glucosidase IMA1 (EC:3.2.1.10)
Alternative name(s):
Alpha-glucosidase
Isomaltase 1
Gene namesi
Name:IMA1
Ordered Locus Names:YGR287C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR287C.
SGDiS000003519. IMA1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi215 – 2151D → A: Abolishes catalytic activity. 1 Publication
Mutagenesisi216 – 2161V → T: Can also hydrolyze maltose. 1 Publication
Mutagenesisi217 – 2171G → A: Does not alter substrate specificity. 1 Publication
Mutagenesisi218 – 2181S → G: Does not alter substrate specificity. 1 Publication

Chemistry

ChEMBLiCHEMBL5848.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 589588Oligo-1,6-glucosidase IMA1PRO_0000054333Add
BLAST

Proteomic databases

MaxQBiP53051.

Expressioni

Inductioni

Expression is increased in response to the addition of maltose, isomaltose, and alpha-methylglucopyranoside.1 Publication

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
RSP5P399402EBI-10464,EBI-16219

Protein-protein interaction databases

BioGridi33537. 35 interactions.
IntActiP53051. 5 interactions.
MINTiMINT-4083809.

Chemistry

BindingDBiP53051.

Structurei

Secondary structure

1
589
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi8 – 103Combined sources
Helixi14 – 174Combined sources
Beta strandi20 – 234Combined sources
Helixi25 – 273Combined sources
Beta strandi31 – 366Combined sources
Helixi39 – 446Combined sources
Helixi46 – 527Combined sources
Beta strandi55 – 595Combined sources
Turni68 – 714Combined sources
Beta strandi75 – 806Combined sources
Turni82 – 843Combined sources
Helixi87 – 9913Combined sources
Beta strandi103 – 1086Combined sources
Helixi118 – 1236Combined sources
Beta strandi127 – 1293Combined sources
Helixi132 – 1343Combined sources
Beta strandi141 – 1433Combined sources
Beta strandi160 – 1678Combined sources
Turni168 – 1714Combined sources
Beta strandi172 – 1754Combined sources
Beta strandi177 – 1793Combined sources
Helixi190 – 20011Combined sources
Helixi202 – 2065Combined sources
Beta strandi211 – 2144Combined sources
Helixi217 – 2193Combined sources
Beta strandi237 – 2393Combined sources
Helixi242 – 2454Combined sources
Helixi251 – 26515Combined sources
Beta strandi273 – 2797Combined sources
Helixi283 – 2908Combined sources
Helixi292 – 2943Combined sources
Beta strandi298 – 3025Combined sources
Helixi304 – 3074Combined sources
Beta strandi314 – 3185Combined sources
Helixi323 – 3319Combined sources
Turni332 – 3343Combined sources
Helixi335 – 3373Combined sources
Helixi356 – 3605Combined sources
Turni365 – 3673Combined sources
Helixi368 – 38013Combined sources
Beta strandi382 – 3898Combined sources
Helixi392 – 3943Combined sources
Helixi404 – 4063Combined sources
Helixi410 – 42314Combined sources
Beta strandi425 – 4273Combined sources
Helixi428 – 44013Combined sources
Helixi442 – 4454Combined sources
Turni455 – 4595Combined sources
Helixi473 – 4775Combined sources
Helixi481 – 4866Combined sources
Helixi491 – 50414Combined sources
Helixi506 – 5094Combined sources
Beta strandi513 – 5197Combined sources
Beta strandi523 – 53210Combined sources
Beta strandi535 – 5428Combined sources
Beta strandi544 – 5463Combined sources
Beta strandi559 – 5668Combined sources
Turni568 – 5703Combined sources
Beta strandi583 – 5886Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3A47X-ray1.59A1-589[»]
3A4AX-ray1.60A1-589[»]
3AJ7X-ray1.30A1-589[»]
3AXHX-ray1.80A1-589[»]
3AXIX-ray1.40A1-589[»]
ProteinModelPortaliP53051.
SMRiP53051. Positions 4-589.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53051.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Phylogenomic databases

GeneTreeiENSGT00530000063127.
HOGENOMiHOG000220641.
InParanoidiP53051.
KOiK01182.
OMAiHLMHGTP.
OrthoDBiEOG092C12JX.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 3 hits.
PfamiPF00128. Alpha-amylase. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53051-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTISSAHPET EPKWWKEATF YQIYPASFKD SNDDGWGDMK GIASKLEYIK
60 70 80 90 100
ELGADAIWIS PFYDSPQDDM GYDIANYEKV WPTYGTNEDC FALIEKTHKL
110 120 130 140 150
GMKFITDLVI NHCSSEHEWF KESRSSKTNP KRDWFFWRPP KGYDAEGKPI
160 170 180 190 200
PPNNWKSYFG GSAWTFDEKT QEFYLRLFCS TQPDLNWENE DCRKAIYESA
210 220 230 240 250
VGYWLDHGVD GFRIDVGSLY SKVVGLPDAP VVDKNSTWQS SDPYTLNGPR
260 270 280 290 300
IHEFHQEMNQ FIRNRVKDGR EIMTVGEMQH ASDETKRLYT SASRHELSEL
310 320 330 340 350
FNFSHTDVGT SPLFRYNLVP FELKDWKIAL AELFRYINGT DCWSTIYLEN
360 370 380 390 400
HDQPRSITRF GDDSPKNRVI SGKLLSVLLS ALTGTLYVYQ GQELGQINFK
410 420 430 440 450
NWPVEKYEDV EIRNNYNAIK EEHGENSEEM KKFLEAIALI SRDHARTPMQ
460 470 480 490 500
WSREEPNAGF SGPSAKPWFY LNDSFREGIN VEDEIKDPNS VLNFWKEALK
510 520 530 540 550
FRKAHKDITV YGYDFEFIDL DNKKLFSFTK KYNNKTLFAA LNFSSDATDF
560 570 580
KIPNDDSSFK LEFGNYPKKE VDASSRTLKP WEGRIYISE
Length:589
Mass (Da):68,592
Last modified:October 1, 1996 - v1
Checksum:i08869180588053B6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti428 – 4281E → D in BAD00094 (PubMed:15291818).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB109221 mRNA. Translation: BAD00094.1.
D43761 Genomic DNA. Translation: BAA07818.1.
Z73072 Genomic DNA. Translation: CAA97319.1.
Z73073 Genomic DNA. Translation: CAA97321.1.
BK006941 Genomic DNA. Translation: DAA08375.1.
PIRiS59370.
RefSeqiNP_011803.3. NM_001181416.3.

Genome annotation databases

EnsemblFungiiYGR287C; YGR287C; YGR287C.
GeneIDi853204.
KEGGisce:YGR287C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB109221 mRNA. Translation: BAD00094.1.
D43761 Genomic DNA. Translation: BAA07818.1.
Z73072 Genomic DNA. Translation: CAA97319.1.
Z73073 Genomic DNA. Translation: CAA97321.1.
BK006941 Genomic DNA. Translation: DAA08375.1.
PIRiS59370.
RefSeqiNP_011803.3. NM_001181416.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3A47X-ray1.59A1-589[»]
3A4AX-ray1.60A1-589[»]
3AJ7X-ray1.30A1-589[»]
3AXHX-ray1.80A1-589[»]
3AXIX-ray1.40A1-589[»]
ProteinModelPortaliP53051.
SMRiP53051. Positions 4-589.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33537. 35 interactions.
IntActiP53051. 5 interactions.
MINTiMINT-4083809.

Chemistry

BindingDBiP53051.
ChEMBLiCHEMBL5848.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.
mycoCLAPiOGL13C_YEAST.

Proteomic databases

MaxQBiP53051.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR287C; YGR287C; YGR287C.
GeneIDi853204.
KEGGisce:YGR287C.

Organism-specific databases

EuPathDBiFungiDB:YGR287C.
SGDiS000003519. IMA1.

Phylogenomic databases

GeneTreeiENSGT00530000063127.
HOGENOMiHOG000220641.
InParanoidiP53051.
KOiK01182.
OMAiHLMHGTP.
OrthoDBiEOG092C12JX.

Enzyme and pathway databases

BioCyciYEAST:YGR287C-MONOMER.
BRENDAi3.2.1.10. 984.
ReactomeiR-SCE-352230. Amino acid transport across the plasma membrane.

Miscellaneous databases

EvolutionaryTraceiP53051.
PROiP53051.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 3 hits.
PfamiPF00128. Alpha-amylase. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMALX3_YEAST
AccessioniPrimary (citable) accession number: P53051
Secondary accession number(s): D6VV64, Q76CZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 752 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.