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Protein

Oligo-1,6-glucosidase IMA1

Gene

IMA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Major isomaltase (alpha-1,6-glucosidase) required for isomaltose utilization. Preferentially hydrolyzes isomaltose, palatinose, and methyl-alpha-glucoside, with little activity towards isomaltotriose or longer oligosaccharides. Does not hydrolyze maltose.2 Publications

Catalytic activityi

Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei215Nucleophile1
Active sitei277Proton donor1
Sitei352Transition state stabilizer1

GO - Molecular functioni

  • oligo-1,6-glucosidase activity Source: SGD
  • sucrose alpha-glucosidase activity Source: SGD

GO - Biological processi

  • disaccharide catabolic process Source: SGD
  • maltose metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Maltose metabolism

Enzyme and pathway databases

BioCyciYEAST:YGR287C-MONOMER.
BRENDAi3.2.1.10. 984.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.
mycoCLAPiOGL13C_YEAST.

Names & Taxonomyi

Protein namesi
Recommended name:
Oligo-1,6-glucosidase IMA1 (EC:3.2.1.10)
Alternative name(s):
Alpha-glucosidase
Isomaltase 1
Gene namesi
Name:IMA1
Ordered Locus Names:YGR287C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR287C.
SGDiS000003519. IMA1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi215D → A: Abolishes catalytic activity. 1 Publication1
Mutagenesisi216V → T: Can also hydrolyze maltose. 1 Publication1
Mutagenesisi217G → A: Does not alter substrate specificity. 1 Publication1
Mutagenesisi218S → G: Does not alter substrate specificity. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL5848.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000543332 – 589Oligo-1,6-glucosidase IMA1Add BLAST588

Proteomic databases

MaxQBiP53051.
PRIDEiP53051.

Expressioni

Inductioni

Expression is increased in response to the addition of maltose, isomaltose, and alpha-methylglucopyranoside.1 Publication

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
RSP5P399402EBI-10464,EBI-16219

Protein-protein interaction databases

BioGridi33537. 37 interactors.
IntActiP53051. 5 interactors.
MINTiMINT-4083809.

Chemistry databases

BindingDBiP53051.

Structurei

Secondary structure

1589
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 10Combined sources3
Helixi14 – 17Combined sources4
Beta strandi20 – 23Combined sources4
Helixi25 – 27Combined sources3
Beta strandi31 – 36Combined sources6
Helixi39 – 44Combined sources6
Helixi46 – 52Combined sources7
Beta strandi55 – 59Combined sources5
Turni68 – 71Combined sources4
Beta strandi75 – 80Combined sources6
Turni82 – 84Combined sources3
Helixi87 – 99Combined sources13
Beta strandi103 – 108Combined sources6
Helixi118 – 123Combined sources6
Beta strandi127 – 129Combined sources3
Helixi132 – 134Combined sources3
Beta strandi141 – 143Combined sources3
Beta strandi160 – 167Combined sources8
Turni168 – 171Combined sources4
Beta strandi172 – 175Combined sources4
Beta strandi177 – 179Combined sources3
Helixi190 – 200Combined sources11
Helixi202 – 206Combined sources5
Beta strandi211 – 214Combined sources4
Helixi217 – 219Combined sources3
Beta strandi237 – 239Combined sources3
Helixi242 – 245Combined sources4
Helixi251 – 265Combined sources15
Beta strandi273 – 279Combined sources7
Helixi283 – 290Combined sources8
Helixi292 – 294Combined sources3
Beta strandi298 – 302Combined sources5
Helixi304 – 307Combined sources4
Beta strandi314 – 318Combined sources5
Helixi323 – 331Combined sources9
Turni332 – 334Combined sources3
Helixi335 – 337Combined sources3
Helixi356 – 360Combined sources5
Turni365 – 367Combined sources3
Helixi368 – 380Combined sources13
Beta strandi382 – 389Combined sources8
Helixi392 – 394Combined sources3
Helixi404 – 406Combined sources3
Helixi410 – 423Combined sources14
Beta strandi425 – 427Combined sources3
Helixi428 – 440Combined sources13
Helixi442 – 445Combined sources4
Turni455 – 459Combined sources5
Helixi473 – 477Combined sources5
Helixi481 – 486Combined sources6
Helixi491 – 504Combined sources14
Helixi506 – 509Combined sources4
Beta strandi513 – 519Combined sources7
Beta strandi523 – 532Combined sources10
Beta strandi535 – 542Combined sources8
Beta strandi544 – 546Combined sources3
Beta strandi559 – 566Combined sources8
Turni568 – 570Combined sources3
Beta strandi583 – 588Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3A47X-ray1.59A1-589[»]
3A4AX-ray1.60A1-589[»]
3AJ7X-ray1.30A1-589[»]
3AXHX-ray1.80A1-589[»]
3AXIX-ray1.40A1-589[»]
ProteinModelPortaliP53051.
SMRiP53051.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53051.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Phylogenomic databases

GeneTreeiENSGT00860000133843.
HOGENOMiHOG000220641.
InParanoidiP53051.
KOiK01182.
OMAiHLMHGTP.
OrthoDBiEOG092C12JX.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 3 hits.
PfamiPF00128. Alpha-amylase. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53051-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTISSAHPET EPKWWKEATF YQIYPASFKD SNDDGWGDMK GIASKLEYIK
60 70 80 90 100
ELGADAIWIS PFYDSPQDDM GYDIANYEKV WPTYGTNEDC FALIEKTHKL
110 120 130 140 150
GMKFITDLVI NHCSSEHEWF KESRSSKTNP KRDWFFWRPP KGYDAEGKPI
160 170 180 190 200
PPNNWKSYFG GSAWTFDEKT QEFYLRLFCS TQPDLNWENE DCRKAIYESA
210 220 230 240 250
VGYWLDHGVD GFRIDVGSLY SKVVGLPDAP VVDKNSTWQS SDPYTLNGPR
260 270 280 290 300
IHEFHQEMNQ FIRNRVKDGR EIMTVGEMQH ASDETKRLYT SASRHELSEL
310 320 330 340 350
FNFSHTDVGT SPLFRYNLVP FELKDWKIAL AELFRYINGT DCWSTIYLEN
360 370 380 390 400
HDQPRSITRF GDDSPKNRVI SGKLLSVLLS ALTGTLYVYQ GQELGQINFK
410 420 430 440 450
NWPVEKYEDV EIRNNYNAIK EEHGENSEEM KKFLEAIALI SRDHARTPMQ
460 470 480 490 500
WSREEPNAGF SGPSAKPWFY LNDSFREGIN VEDEIKDPNS VLNFWKEALK
510 520 530 540 550
FRKAHKDITV YGYDFEFIDL DNKKLFSFTK KYNNKTLFAA LNFSSDATDF
560 570 580
KIPNDDSSFK LEFGNYPKKE VDASSRTLKP WEGRIYISE
Length:589
Mass (Da):68,592
Last modified:October 1, 1996 - v1
Checksum:i08869180588053B6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti428E → D in BAD00094 (PubMed:15291818).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB109221 mRNA. Translation: BAD00094.1.
D43761 Genomic DNA. Translation: BAA07818.1.
Z73072 Genomic DNA. Translation: CAA97319.1.
Z73073 Genomic DNA. Translation: CAA97321.1.
BK006941 Genomic DNA. Translation: DAA08375.1.
PIRiS59370.
RefSeqiNP_011803.3. NM_001181416.3.

Genome annotation databases

EnsemblFungiiYGR287C; YGR287C; YGR287C.
GeneIDi853204.
KEGGisce:YGR287C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB109221 mRNA. Translation: BAD00094.1.
D43761 Genomic DNA. Translation: BAA07818.1.
Z73072 Genomic DNA. Translation: CAA97319.1.
Z73073 Genomic DNA. Translation: CAA97321.1.
BK006941 Genomic DNA. Translation: DAA08375.1.
PIRiS59370.
RefSeqiNP_011803.3. NM_001181416.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3A47X-ray1.59A1-589[»]
3A4AX-ray1.60A1-589[»]
3AJ7X-ray1.30A1-589[»]
3AXHX-ray1.80A1-589[»]
3AXIX-ray1.40A1-589[»]
ProteinModelPortaliP53051.
SMRiP53051.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33537. 37 interactors.
IntActiP53051. 5 interactors.
MINTiMINT-4083809.

Chemistry databases

BindingDBiP53051.
ChEMBLiCHEMBL5848.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.
mycoCLAPiOGL13C_YEAST.

Proteomic databases

MaxQBiP53051.
PRIDEiP53051.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR287C; YGR287C; YGR287C.
GeneIDi853204.
KEGGisce:YGR287C.

Organism-specific databases

EuPathDBiFungiDB:YGR287C.
SGDiS000003519. IMA1.

Phylogenomic databases

GeneTreeiENSGT00860000133843.
HOGENOMiHOG000220641.
InParanoidiP53051.
KOiK01182.
OMAiHLMHGTP.
OrthoDBiEOG092C12JX.

Enzyme and pathway databases

BioCyciYEAST:YGR287C-MONOMER.
BRENDAi3.2.1.10. 984.

Miscellaneous databases

EvolutionaryTraceiP53051.
PROiP53051.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 3 hits.
PfamiPF00128. Alpha-amylase. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMALX3_YEAST
AccessioniPrimary (citable) accession number: P53051
Secondary accession number(s): D6VV64, Q76CZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 752 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.