Reviewed, UniProtKB/Swiss-Prot P53051 (MALX3_YEAST)

Names and origin

Protein names

Recommended name:
    Probable alpha-glucosidase FSP2
    EC=3.2.1.20
Alternative name(s):
    Maltase
    Flocculent-specific protein 2

Gene names

Name:	FSP2
Ordered Locus Names:	YGR287C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	589 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probable alpha-glucosidase involved in maltose metabolism.
Catalytic activity	Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.
Miscellaneous	Present with 752 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Maltose metabolism
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	maltose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from direct assay. Source: SGD
Molecular function	alpha-glucosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ESS1	P22696	1	EBI-10464,EBI-6679
PRP40	P33203	1	EBI-10464,EBI-701
RSP5	P39940	1	EBI-10464,EBI-16219
SSM4	P40318	1	EBI-10464,EBI-18208
URN1	Q06525	1	EBI-10464,EBI-35138

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 589	589	Probable alpha-glucosidase FSP2		PRO_0000054333
Sites
	Active site	215	1	Nucleophile By similarity
	Active site	277	1	Proton donor By similarity
	Active site	352	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P53051-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 08869180588053B6	FASTA	589	68,592
	10 20 30 40 50 60 MTISSAHPET EPKWWKEATF YQIYPASFKD SNDDGWGDMK GIASKLEYIK ELGADAIWIS 70 80 90 100 110 120 PFYDSPQDDM GYDIANYEKV WPTYGTNEDC FALIEKTHKL GMKFITDLVI NHCSSEHEWF 130 140 150 160 170 180 KESRSSKTNP KRDWFFWRPP KGYDAEGKPI PPNNWKSYFG GSAWTFDEKT QEFYLRLFCS 190 200 210 220 230 240 TQPDLNWENE DCRKAIYESA VGYWLDHGVD GFRIDVGSLY SKVVGLPDAP VVDKNSTWQS 250 260 270 280 290 300 SDPYTLNGPR IHEFHQEMNQ FIRNRVKDGR EIMTVGEMQH ASDETKRLYT SASRHELSEL 310 320 330 340 350 360 FNFSHTDVGT SPLFRYNLVP FELKDWKIAL AELFRYINGT DCWSTIYLEN HDQPRSITRF 370 380 390 400 410 420 GDDSPKNRVI SGKLLSVLLS ALTGTLYVYQ GQELGQINFK NWPVEKYEDV EIRNNYNAIK 430 440 450 460 470 480 EEHGENSEEM KKFLEAIALI SRDHARTPMQ WSREEPNAGF SGPSAKPWFY LNDSFREGIN 490 500 510 520 530 540 VEDEIKDPNS VLNFWKEALK FRKAHKDITV YGYDFEFIDL DNKKLFSFTK KYNNKTLFAA 550 560 570 580 LNFSSDATDF KIPNDDSSFK LEFGNYPKKE VDASSRTLKP WEGRIYISE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Tonouchi A. Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence analysis of a near-subtelomeric 35.4 kb DNA segment on the right arm of chromosome VII from Saccharomyces cerevisiae carrying the MAL1 locus reveals 15 complete open reading frames, including ZUO1, BGL2 and BIO2 genes and an ABC transporter gene." Volckaert G., Voet M., Robben J. Yeast 13:251-259(1997) [PubMed: 9090054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D43761 Genomic DNA. Translation: BAA07818.1. Z73072 Genomic DNA. Translation: CAA97319.1. Z73073 Genomic DNA. Translation: CAA97321.1.
PIR	S59370.
RefSeq	NP_011803.1.
3D structure databases
HSSP	HSSP built from PDB template 1UOK based on UniProtKB P21332.
ModBase	Search...
Protein-protein interaction databases
IntAct	P53051.
Genome annotation databases
Ensembl	YGR287C. Saccharomyces cerevisiae. [Contig view]
GeneID	853204.
GenomeReviews	Gene locus YGR287C in contig Y13135_GR.
KEGG	sce:YGR287C.
NMPDR	fig|4932.3.peg.2934.
Organism-specific databases
CYGD	YGR287c.
SGD	S000003519. YGR287C.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P53051.
Gene expression databases
ArrayExpress	P53051.
GermOnline	YGR287C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat. IPR006589. Glyco_hydro_13_sub_cat. IPR013781. Glyco_hydro_sub_cat. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. [Graphical view]
SMART	SM00642. Aamy. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	P53051.
NextBio	973383.

Entry information

Entry name

MALX3_YEAST

Accession

Primary (citable) accession number: P53051

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 25, 2008
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)