General alpha-glucoside permease - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P53048 (MAL11_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 113. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
General alpha-glucoside permease

Alternative name(s):
Maltose permease MAL11
Maltose transport protein MAL11

Gene names

Name:	MAL11
Synonyms:	AGT1, MAL1T, MTP1
Ordered Locus Names:	YGR289C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	616 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	High-affinity uptake of alpha-glucosides such as maltose, turanose, isomaltose, alpha-methylglucoside, maltotriose, palatinose, trehalose, melezitose and glucose. Acts with the concomitant transport of protons into the cell (symport system). Ref.1 Ref.5 Ref.6
Subcellular location	Membrane; Multi-pass membrane protein.
Induction	By maltose and maltotriose. Repressed by glucose. Ref.1 Ref.5 Ref.6
Sequence similarities	Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. [View classification]

Ontologies

Keywords
Biological process	Maltose metabolism Sugar transport Symport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	disaccharide catabolic process Inferred from mutant phenotype PubMed 20562106. Source: SGD maltose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD plasma membrane Inferred from sequence or structural similarity PubMed 16741702 Ref.1. Source: SGD
Molecular_function	alpha-glucoside:hydrogen symporter activity Inferred from direct assay PubMed 12702465. Source: SGD maltose:hydrogen symporter activity Inferred from mutant phenotype PubMed 1999393 Ref.1. Source: SGD trehalose transmembrane transporter activity Inferred from direct assay PubMed 12702465. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 616

616

General alpha-glucoside permease

PRO_0000050425

Regions

Topological domain

1 – 115

115

Cytoplasmic Potential

Transmembrane

116 – 136

Helical; Name=1; Potential

Topological domain

137 – 150

Extracellular Potential

Transmembrane

151 – 171

Helical; Name=2; Potential

Topological domain

172 – 186

Cytoplasmic Potential

Transmembrane

187 – 207

Helical; Name=3; Potential

Topological domain

208

Extracellular Potential

Transmembrane

209 – 229

Helical; Name=4; Potential

Topological domain

230 – 242

Cytoplasmic Potential

Transmembrane

243 – 263

Helical; Name=5; Potential

Topological domain

264 – 278

Extracellular Potential

Transmembrane

279 – 299

Helical; Name=6; Potential

Topological domain

300 – 370

Cytoplasmic Potential

Transmembrane

371 – 391

Helical; Name=7; Potential

Topological domain

392 – 404

Extracellular Potential

Transmembrane

405 – 425

Helical; Name=8; Potential

Topological domain

426 – 433

Cytoplasmic Potential

Transmembrane

434 – 454

Helical; Name=9; Potential

Topological domain

455 – 466

Extracellular Potential

Transmembrane

467 – 487

Helical; Name=10; Potential

Topological domain

488 – 499

Cytoplasmic Potential

Transmembrane

500 – 520

Helical; Name=11; Potential

Topological domain

521 – 532

Extracellular Potential

Transmembrane

533 – 553

Helical; Name=12; Potential

Topological domain

554 – 616

Cytoplasmic Potential

Amino acid modifications

Glycosylation

523

N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict

396 – 398

RAG → KKQV in AAB07600. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P53048 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: EE84AD9E15F7926D
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FASTA

616

67,980

        10         20         30         40         50         60 
MKNIISLVSK KKAASKNEDK NISESSRDIV NQQEVFNTED FEEGKKDSAF ELDHLEFTTN 

        70         80         90        100        110        120 
SAQLGDSDED NENVINEMNA TDDANEANSE EKSMTLKQAL LKYPKAALWS ILVSTTLVME 

       130        140        150        160        170        180 
GYDTALLSAL YALPVFQRKF GTLNGEGSYE ITSQWQIGLN MCVLCGEMIG LQITTYMVEF 

       190        200        210        220        230        240 
MGNRYTMITA LGLLTAYIFI LYYCKSLAMI AVGQILSAIP WGCFQSLAVT YASEVCPLAL 

       250        260        270        280        290        300 
RYYMTSYSNI CWLFGQIFAS GIMKNSQENL GNSDLGYKLP FALQWIWPAP LMIGIFFAPE 

       310        320        330        340        350        360 
SPWWLVRKDR VAEARKSLSR ILSGKGAEKD IQVDLTLKQI ELTIEKERLL ASKSGSFFNC 

       370        380        390        400        410        420 
FKGVNGRRTR LACLTWVAQN SSGAVLLGYS TYFFERAGMA TDKAFTFSLI QYCLGLAGTL 

       430        440        450        460        470        480 
CSWVISGRVG RWTILTYGLA FQMVCLFIIG GMGFGSGSSA SNGAGGLLLA LSFFYNAGIG 

       490        500        510        520        530        540 
AVVYCIVAEI PSAELRTKTI VLARICYNLM AVINAILTPY MLNVSDWNWG AKTGLYWGGF 

       550        560        570        580        590        600 
TAVTLAWVII DLPETTGRTF SEINELFNQG VPARKFASTV VDPFGKGKTQ HDSLADESIS 

       610 
QSSSIKQREL NAADKC

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of AGT1 encoding a general alpha-glucoside transporter from Saccharomyces." Han E.-K., Cotty F., Sottas C., Jiang H., Michels C.A. Mol. Microbiol. 17:1093-1107(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION.
[2]	"Sequence analysis of a near-subtelomeric 35.4 kb DNA segment on the right arm of chromosome VII from Saccharomyces cerevisiae carrying the MAL1 locus reveals 15 complete open reading frames, including ZUO1, BGL2 and BIO2 genes and an ABC transporter gene." Volckaert G., Voet M., Robben J. Yeast 13:251-259(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Concurrent knock-out of at least 20 transporter genes is required to block uptake of hexoses in Saccharomyces cerevisiae." Wieczorke R., Krampe S., Weierstall T., Freidel K., Hollenberg C.P., Boles E. FEBS Lett. 464:123-128(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION.
[6]	"Characterization of the putative maltose transporters encoded by YDL247w and YJR160c." Day R.E., Higgins V.J., Rogers P.J., Dawes I.W. Yeast 19:1015-1027(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION.
[7]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L47346 Genomic DNA. Translation: AAB07600.1. Z73074 Genomic DNA. Translation: CAA97322.1. BK006941 Genomic DNA. Translation: DAA08377.1.
PIR	S64624.
RefSeq	NP_011805.3. NM_001181418.3.
3D structure databases
ProteinModelPortal	P53048.
SMR	P53048. Positions 214-567.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5567N.
IntAct	P53048. 1 interaction.
MINT	MINT-552607.
Protein family/group databases
TCDB	2.A.1.1.11. major facilitator superfamily (MFS).
Proteomic databases
PaxDb	P53048.
PeptideAtlas	P53048.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR289C; YGR289C; YGR289C.
GeneID	853207.
KEGG	sce:YGR289C.
Organism-specific databases
CYGD	YGR289c.
SGD	S000003521. MAL11.
Phylogenomic databases
eggNOG	COG0477.
GeneTree	ENSGT00650000093912.
HOGENOM	HOG000159003.
KO	K08141.
OMA	RTEIVCM.
OrthoDB	EOG454D72.
Gene expression databases
ArrayExpress	P53048.
Genevestigator	P53048.
GermOnline	YGR289C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR020846. MFS_dom. IPR016196. MFS_dom_general_subst_transpt. IPR005828. Sub_transporter. IPR003663. Sugar/inositol_transpt. [Graphical view]
Pfam	PF00083. Sugar_tr. 1 hit. [Graphical view]
SUPFAM	SSF103473. MFS_gen_substrate_transporter. 1 hit.
TIGRFAMs	TIGR00879. SP. 1 hit.
PROSITE	PS50850. MFS. 1 hit. PS00216. SUGAR_TRANSPORT_1. False negative. PS00217. SUGAR_TRANSPORT_2. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	973389.

Entry information

Entry name

MAL11_YEAST

Accession

Primary (citable) accession number: P53048
Secondary accession number(s): D6VV66

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 113 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents